Butylated hydroxytoluene

Name: Butylated hydroxytoluene
Brand: MedChemExpress
SKU: HY-Y0172
Rating: 5.0 (4 reviews)

Cat. No.: HY-Y0172 Purity: 99.74%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Butylated hydroxytoluene is an antioxidant widely used in foods and in food-related products. Butylated hydroxytoluene is a Ferroptosis inhibitor.

For research use only. We do not sell to patients.

CAS No. : 128-37-0

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Butylated hydroxytoluene:

Butylated hydroxytoluene-d₂₁ In-stock
Butylated hydroxytoluene-d₃ In-stock
Butylated hydroxytoluene-d₂₄ Get quote
Butylated hydroxytoluene (Standard) Get quote

4 Publications Citing Use of MCE Butylated hydroxytoluene

Cell Proliferation/Viability Assay

Bio/Physico-chemical Assay

Flow Cytometry

Cell Imaging/Staining

: Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]; Viability of HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

: Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]; MDA levels in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

: Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]; 4-HNE levels in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

: Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]; ROS levels in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

: Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]; Representative images of C11-BODIPY probe in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Butylated hydroxytoluene is an antioxidant widely used in foods and in food-related products^[1]. Butylated hydroxytoluene is a Ferroptosis inhibitor^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	3.18 μM Compound: table 1, 31(BHT)	Induction of DNA fragmentation in human HL60 cells after 16 hrs Induction of DNA fragmentation in human HL60 cells after 16 hrs	[PMID: 16279782]
Hepatocyte	IC₅₀	1.7 μM Compound: 26	Tested for inhibition of ferrous salt/ascorbate induced lipidic peroxidation of membrane lipid of rat hepatocytes Tested for inhibition of ferrous salt/ascorbate induced lipidic peroxidation of membrane lipid of rat hepatocytes	[PMID: 15546710]
L1210	IC₅₀	91.2 μM Compound: table 1, 31(BHT)	Cytotoxicity against mouse L1210 cells Cytotoxicity against mouse L1210 cells	[PMID: 16279782]
Platelet	IC₅₀	14.5 μM Compound: butylated hydroxy toulene	Antiplatelet activity against rabbit platelet assessed as inhibition of platelet-activating factor-induced platelet aggregation at 20 uM preincubated 3 mins before PAF challenge by turbidimetric method relative to control Antiplatelet activity against rabbit platelet assessed as inhibition of platelet-activating factor-induced platelet aggregation at 20 uM preincubated 3 mins before PAF challenge by turbidimetric method relative to control	[PMID: 9358644]

In Vivo

Butylhydroxytoluene (BHT) is well-known as a potent promoter of carcinogen-induced lung tumors in mice. Butylated hydroxytoluene (orally; 400 mg/kg; weekly) administration increases the susceptibility of 7-week-old rasH2 mice to lung carcinogens^[3]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

220.36

Formula

C₁₅H₂₄O

CAS No.

128-37-0

Appearance

Solid

Color

White to light yellow

SMILES

OC1=C(C(C)(C)C)C=C(C)C=C1C(C)(C)C

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, stored under nitrogen

In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (453.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.5380 mL	22.6901 mL	45.3803 mL
5 mM	0.9076 mL	4.5380 mL	9.0761 mL
10 mM	0.4538 mL	2.2690 mL	4.5380 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (11.35 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (453.80 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (274 KB) SDS (623 KB)

COA (249 KB) HNMR (109 KB) GC (195 KB)

Handling Instructions (2659 KB)

References

[1]. Babich H, et al. Butylated hydroxytoluene (BHT): a review. Environ Res. 1982 Oct;29(1):1-29. [Content Brief]

[2]. Stockwell BR, et al. Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease. ell. 2017 Oct 5;171(2):273-285. [Content Brief]

[3]. Umemura T, et al. Butylhydroxytoluene (BHT) increases susceptibility of transgenic rasH2 mice to lung carcinogenesis. J Cancer Res Clin Oncol. 2001 Oct;127(10):583-90. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.5380 mL	22.6901 mL	45.3803 mL	113.4507 mL
	5 mM	0.9076 mL	4.5380 mL	9.0761 mL	22.6901 mL
	10 mM	0.4538 mL	2.2690 mL	4.5380 mL	11.3451 mL
	15 mM	0.3025 mL	1.5127 mL	3.0254 mL	7.5634 mL
	20 mM	0.2269 mL	1.1345 mL	2.2690 mL	5.6725 mL
	25 mM	0.1815 mL	0.9076 mL	1.8152 mL	4.5380 mL
	30 mM	0.1513 mL	0.7563 mL	1.5127 mL	3.7817 mL
	40 mM	0.1135 mL	0.5673 mL	1.1345 mL	2.8363 mL
	50 mM	0.0908 mL	0.4538 mL	0.9076 mL	2.2690 mL
	60 mM	0.0756 mL	0.3782 mL	0.7563 mL	1.8908 mL
	80 mM	0.0567 mL	0.2836 mL	0.5673 mL	1.4181 mL
	100 mM	0.0454 mL	0.2269 mL	0.4538 mL	1.1345 mL