Butylated hydroxytoluene
Based on 6 publication(s) in Google Scholar
Butylated hydroxytoluene is an antioxidant widely used in foods and in food-related products. Butylated hydroxytoluene is a Ferroptosis inhibitor.
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- Pureza : 99.9%
- No. CAS: 128-37-0
- Fòrmula: C15H24O
- Peso molecular:220.36
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Almacenamiento:
RT, stored under nitrogen.
In solvent -80°C, 1 year , -20°C, 6 months
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Butylated hydroxytoluene
More- Nature. 2026 Jul;655(8121):240-250. [Abstract]
- Anal Chem. 2026 Mar 3;98(8):5964-5975. [Abstract]
- Colloids Surf B Biointerfaces. 2025 Oct:254:114864. [Abstract]
- Int Immunopharmacol. 2024 Sep 10:138:112518. [Abstract]
- FASEB J. 2026 May 31;40(10):e71812. [Abstract]
- Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Flow Cytometry
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Cell Imaging/Staining
Ver todos los productos específicos de isoformas Endogenous Metabolite
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Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Hepatocyte | IC50 |
1.7 μM
Compound: 26
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Tested for inhibition of ferrous salt/ascorbate induced lipidic peroxidation of membrane lipid of rat hepatocytes
Tested for inhibition of ferrous salt/ascorbate induced lipidic peroxidation of membrane lipid of rat hepatocytes
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[PMID: 15546710] |
| HL-60 | IC50 |
3.18 μM
Compound: table 1, 31(BHT)
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Induction of DNA fragmentation in human HL60 cells after 16 hrs
Induction of DNA fragmentation in human HL60 cells after 16 hrs
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[PMID: 16279782] |
| L1210 | IC50 |
91.2 μM
Compound: table 1, 31(BHT)
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Cytotoxicity against mouse L1210 cells
Cytotoxicity against mouse L1210 cells
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[PMID: 16279782] |
| Platelet | IC50 |
14.5 μM
Compound: butylated hydroxy toulene
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Antiplatelet activity against rabbit platelet assessed as inhibition of platelet-activating factor-induced platelet aggregation at 20 uM preincubated 3 mins before PAF challenge by turbidimetric method relative to control
Antiplatelet activity against rabbit platelet assessed as inhibition of platelet-activating factor-induced platelet aggregation at 20 uM preincubated 3 mins before PAF challenge by turbidimetric method relative to control
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[PMID: 9358644] |
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 128-37-0
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Appearance Solid
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Peso molecular 220.36
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Fòrmula C15H24O
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Color White to light yellow
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SMILES
OC1=C(C(C)(C)C)C=C(C)C=C1C(C)(C)C
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
RT, stored under nitrogen
In solvent -80°C 1 year -20°C 6 months
Publications (6)
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Journal Impact Factor
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Most Recent
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Nature
2026 Jul;655(8121):240-250. PMID: 42236947 -
Anal Chem
Universal Enrichment and Sequencing of DNA Modifications Using a Click-Coupled IgG-Protein A/G System. [Abstract]2026 Mar 3;98(8):5964-5975. PMID: 41693615 -
Colloids Surf B Biointerfaces
Single-aggregate morphological and biomechanical detection of atherogenic aggregated low-density lipoprotein (agLDL) by atomic force microscopy. [Abstract]2025 Oct:254:114864. PMID: 40505502 -
Int Immunopharmacol
Targeting lipid peroxidation-associated ferroptosis suppresses lung carcinoma progression by regulating cell cycle arrest. [Abstract]2024 Sep 10:138:112518. PMID: 38917528 -
FASEB J
Atf4a Regulates Mitochondrial Homeostasis Through Parkin-Mediated Mitophagy to Enhance Hypoxia Tolerance in Zebrafish (Danio rerio). [Abstract]2026 May 31;40(10):e71812. PMID: 42118049 -
Biomed Pharmacother
Gingerenone A induces ferroptosis in colorectal cancer via targeting suppression of SLC7A11 signaling pathway. [Abstract]2024 Nov:180:117529. PMID: 39393329
Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]
Viability of HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.
Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]
MDA levels in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.
Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]
4-HNE levels in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.
Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]
ROS levels in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.
Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]
Representative images of C11-BODIPY probe in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (453.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (11.35 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (453.80 mM); Clear solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
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Ferroptosis Solutions
Ferroptosis is an iron-dependent, non-apoptotic form of regulated cell death characterized by lethal lipid peroxidation and sensitivity to suppression by iron chelators or lipophilic radical-trapping antioxidants. The core pathway links cystine uptake through system Xc−, glutathione availability, GPX4-dependent detoxification of phospholipid hydroperoxides, iron-dependent oxidative reactions, and polyunsaturated-phospholipid metabolism into a cell-death program that is biochemically and morphologically distinct from apoptosis, necrosis, and autophagy. The ferroptosis pathway is experimentally linked to phenotype through chemical and genetic perturbation. Erastin induces ferroptosis by inhibiting cystine uptake through system Xc− and weakening antioxidant defenses, while GPX4 inhibition or depletion causes lipid peroxide accumulation and ferroptotic cancer-cell death. ACSL4 and oxidizable arachidonoyl- or adrenoyl-containing phosphatidylethanolamines shape ferroptosis sensitivity by con
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (623 KB)
- English - EN (623 KB)
- Français - FR (623 KB)
- Deutsch - DE (623 KB)
- Norwegian - NO (623 KB)
- Español - ES (623 KB)
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- Italian - IT (623 KB)
- Korean - KR (623 KB)
- Portuguese - PT (623 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Babich H, et al. Butylated hydroxytoluene (BHT): a review. Environ Res. 1982 Oct;29(1):1-29. [Content Brief]
[2]. Stockwell BR, et al. Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease. ell. 2017 Oct 5;171(2):273-285. [Content Brief]
[3]. Umemura T, et al. Butylhydroxytoluene (BHT) increases susceptibility of transgenic rasH2 mice to lung carcinogenesis. J Cancer Res Clin Oncol. 2001 Oct;127(10):583-90. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.5380 mL | 22.6901 mL | 45.3803 mL | 113.4507 mL |
| 5 mM | 0.9076 mL | 4.5380 mL | 9.0761 mL | 22.6901 mL | |
| 10 mM | 0.4538 mL | 2.2690 mL | 4.5380 mL | 11.3451 mL | |
| 15 mM | 0.3025 mL | 1.5127 mL | 3.0254 mL | 7.5634 mL | |
| 20 mM | 0.2269 mL | 1.1345 mL | 2.2690 mL | 5.6725 mL | |
| 25 mM | 0.1815 mL | 0.9076 mL | 1.8152 mL | 4.5380 mL | |
| 30 mM | 0.1513 mL | 0.7563 mL | 1.5127 mL | 3.7817 mL | |
| 40 mM | 0.1135 mL | 0.5673 mL | 1.1345 mL | 2.8363 mL | |
| 50 mM | 0.0908 mL | 0.4538 mL | 0.9076 mL | 2.2690 mL | |
| 60 mM | 0.0756 mL | 0.3782 mL | 0.7563 mL | 1.8908 mL | |
| 80 mM | 0.0567 mL | 0.2836 mL | 0.5673 mL | 1.4181 mL | |
| 100 mM | 0.0454 mL | 0.2269 mL | 0.4538 mL | 1.1345 mL |
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