Cat. No.: HY-Y0172

Butylated hydroxytoluene

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Butylated hydroxytoluene is an antioxidant widely used in foods and in food-related products. Butylated hydroxytoluene is a Ferroptosis inhibitor.

For research use only. We do not sell to patients.

Purity: 99.74%
CAS No.: 128-37-0
Formula: C₁₅H₂₄O
Molecular Weight:220.36

Storage:

RT, stored under nitrogen.

In solvent -80°C, 1 year , -20°C, 6 months

Butylated hydroxytoluene Chemical Structure

CAS No.: 128-37-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vivo
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Butylated hydroxytoluene is an antioxidant widely used in foods and in food-related products^[1]. Butylated hydroxytoluene is a Ferroptosis inhibitor^[2].

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Hepatocyte	IC₅₀	1.7 μM Compound: 26	Tested for inhibition of ferrous salt/ascorbate induced lipidic peroxidation of membrane lipid of rat hepatocytes Tested for inhibition of ferrous salt/ascorbate induced lipidic peroxidation of membrane lipid of rat hepatocytes	[PMID: 15546710]
HL-60	IC₅₀	3.18 μM Compound: table 1, 31(BHT)	Induction of DNA fragmentation in human HL60 cells after 16 hrs Induction of DNA fragmentation in human HL60 cells after 16 hrs	[PMID: 16279782]
L1210	IC₅₀	91.2 μM Compound: table 1, 31(BHT)	Cytotoxicity against mouse L1210 cells Cytotoxicity against mouse L1210 cells	[PMID: 16279782]
Platelet	IC₅₀	14.5 μM Compound: butylated hydroxy toulene	Antiplatelet activity against rabbit platelet assessed as inhibition of platelet-activating factor-induced platelet aggregation at 20 uM preincubated 3 mins before PAF challenge by turbidimetric method relative to control Antiplatelet activity against rabbit platelet assessed as inhibition of platelet-activating factor-induced platelet aggregation at 20 uM preincubated 3 mins before PAF challenge by turbidimetric method relative to control	[PMID: 9358644]

In Vivo

Butylhydroxytoluene (BHT) is well-known as a potent promoter of carcinogen-induced lung tumors in mice. Butylated hydroxytoluene (orally; 400 mg/kg; weekly) administration increases the susceptibility of 7-week-old rasH2 mice to lung carcinogens^[3]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 128-37-0
Appearance Solid
Molecular Weight 220.36
Formula C₁₅H₂₄O
Color White to light yellow
SMILES

OC1=C(C(C)(C)C)C=C(C)C=C1C(C)(C)C
Structure Classification
- Phenols
- Monophenols
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, stored under nitrogen

In solvent	-80°C	1 year
	-20°C	6 months

Publications (6)

All Cell Proliferation/Viability AssayBio/Physico-chemical AssayFlow CytometryCell Imaging/Staining

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Journal Impact Factor

Nature

Spermine is an endogenous iron chelator that inhibits ferroptosis. [Abstract]

2026 Jul;655(8121):240-250. PMID: 42236947
Biomed Pharmacother

Gingerenone A induces ferroptosis in colorectal cancer via targeting suppression of SLC7A11 signaling pathway. [Abstract]

2024 Nov:180:117529. PMID: 39393329

Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]

Viability of HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]

MDA levels in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]

4-HNE levels in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]

ROS levels in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.

Butylated hydroxytoluene purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Nov:180:117529. [Abstract]

Representative images of C11-BODIPY probe in HCT-15 cells treated with GA with or without Fer-1 and Butylated hydroxytoluene (BHT) for 24 h.
Anal Chem

Universal Enrichment and Sequencing of DNA Modifications Using a Click-Coupled IgG-Protein A/G System. [Abstract]

2026 Mar 3;98(8):5964-5975. PMID: 41693615
Colloids Surf B Biointerfaces

Single-aggregate morphological and biomechanical detection of atherogenic aggregated low-density lipoprotein (agLDL) by atomic force microscopy. [Abstract]

2025 Jun 7:254:114864. PMID: 40505502
Int Immunopharmacol

Targeting lipid peroxidation-associated ferroptosis suppresses lung carcinoma progression by regulating cell cycle arrest. [Abstract]

2024 Jun 24:138:112518. PMID: 38917528
FASEB J

Atf4a Regulates Mitochondrial Homeostasis Through Parkin-Mediated Mitophagy to Enhance Hypoxia Tolerance in Zebrafish (Danio rerio). [Abstract]

2026 May 31;40(10):e71812. PMID: 42118049

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (453.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.5380 mL

22.6901 mL

45.3803 mL

5 mM

0.9076 mL

4.5380 mL

9.0761 mL

10 mM

0.4538 mL

2.2690 mL

4.5380 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (11.35 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (11.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (11.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (453.80 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (623 KB)
- English - EN (623 KB)
- Français - FR (623 KB)
- Deutsch - DE (623 KB)
- Norwegian - NO (623 KB)
- Español - ES (623 KB)
- Swedish - SV (623 KB)
- Italian - IT (623 KB)
- Korean - KR (623 KB)
- Portuguese - PT (623 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Babich H, et al. Butylated hydroxytoluene (BHT): a review. Environ Res. 1982 Oct;29(1):1-29. [Content Brief]

[2]. Stockwell BR, et al. Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease. ell. 2017 Oct 5;171(2):273-285. [Content Brief]

[3]. Umemura T, et al. Butylhydroxytoluene (BHT) increases susceptibility of transgenic rasH2 mice to lung carcinogenesis. J Cancer Res Clin Oncol. 2001 Oct;127(10):583-90. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.5380 mL	22.6901 mL	45.3803 mL	113.4507 mL
	5 mM	0.9076 mL	4.5380 mL	9.0761 mL	22.6901 mL
	10 mM	0.4538 mL	2.2690 mL	4.5380 mL	11.3451 mL
	15 mM	0.3025 mL	1.5127 mL	3.0254 mL	7.5634 mL
	20 mM	0.2269 mL	1.1345 mL	2.2690 mL	5.6725 mL
	25 mM	0.1815 mL	0.9076 mL	1.8152 mL	4.5380 mL
	30 mM	0.1513 mL	0.7563 mL	1.5127 mL	3.7817 mL
	40 mM	0.1135 mL	0.5673 mL	1.1345 mL	2.8363 mL
	50 mM	0.0908 mL	0.4538 mL	0.9076 mL	2.2690 mL
	60 mM	0.0756 mL	0.3782 mL	0.7563 mL	1.8908 mL
	80 mM	0.0567 mL	0.2836 mL	0.5673 mL	1.4181 mL
	100 mM	0.0454 mL	0.2269 mL	0.4538 mL	1.1345 mL

Keywords

Butylated hydroxytoluene

6 Cited Publications

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Butylated hydroxytoluene

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Publications Citing Use of MedChemExpress (MCE) Butylated hydroxytoluene

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Butylated hydroxytoluene Related Classifications

HY-Y0172 Related Classifications