RhoNox-4
Based on 1 Customer Validation
RhoNox-4 is a Fe2+-selective fluorescence-activatable probe and an inhibitor of ferroptosis. RhoNox-4 shows no obvious cytotoxicity even at concentrations up to 50 μM. RhoNox-4 can selectively oxidize labile Fe2+ to Fe3+, depleting the iron pool required for lipid peroxidation and ferroptosis. RhoNox-4 inhibits ferroptosis induced by ferroptosis inducers; its activity depends on localization to the endoplasmic reticulum/Golgi apparatus, and it does not affect apoptosis or necrosis. RhoNox-4 can be used for imaging labile Fe2+ in living cells, and also enables high-throughput screening of labile iron homeostasis regulators based on 96-well plates. RhoNox-4 allows visualization of cytoplasmic Fe2+ levels in root tips of *Arabidopsis* (Ex/Em = 530-560/573-647 nm).
For research use only. We do not sell to patients.
- Purity: 98.65%
- CAS No.: 2711762-70-6
- Formula: C38H44N4O8
- Molecular Weight:684.78
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
RhoNox-4 (2 μM; up to 3000 seconds) shows rapid, highly sensitive fluorescence turn-on response to Fe2+ in cell-free buffer, with >100-fold fluorescence enhancement within 15 minutes[1].
RhoNox-4 (1 μM) is a cell-permeable Fe2+-selective fluorescent probe that produces high-contrast fluorescence signals in Fe2+-treated HepG2 cells[1].
RhoNox-4 supports high-throughput screening for compounds modulating subcellular labile iron levels, identifying lomofungin as a novel ferritin-downregulating labile iron upregulator[1].
RhoNox-4 (3.2-12.8 μM) inhibits Erastin (HY-15763)-induced ferroptosis in HT1080 cells, maintaining ~90% cell viability at 3.2 μM and ~80% cell viability at 12.8 μM[1].
RhoNox-4 (0.2-12.8 μM; 12 h) potently inhibits Erastin-induced ferroptosis in HT1080 cells with an IC50 of approximately 3.5 μM[2].
RhoNox-4 (0.2-12.8 μM; 12 h) robustly inhibits RSL3 (HY-100218A)-induced ferroptosis in HT1080 cells, reducing cell death by more than 50%[2].
RhoNox-4 (12.8 μM; 12 h) inhibits FIN56 (HY-103087)- and FINO2 (HY-129457)-induced ferroptosis in HT1080 cells, providing effective cell viability rescue[2].
RhoNox-4 (12.8 μM; 12 h, 24 h) does not protect HT1080 cells from necrosis or apoptosis, demonstrating selective inhibition of ferroptosis[2].
RhoNox-4 (1.0 h, 6 h) detects elevated labile Fe2+ levels in erastin-treated HT1080 cells[2].
RhoNox-4 (2 μM; 1 h) acts as a highly selective, turn-on fluorescent probe for Fe2+ in cell-free conditions, with an off/on ratio of 160 and an apparent reaction rate of 1.0 × 10-3 s-1[3].
RhoNox-4 (1 μM; 30 min) efficiently detects labile Fe2+ in live HepG2 cells, with highest sensitivity in the endoplasmic reticulum and mitochondria, and shows negligible cytotoxicity up to 50 μM[3].
RhoNox-4 (5 µM; 3 h incubation) labels cytosolic Fe2+ in Arabidopsis thaliana root tips, revealing that Split +Fe root tips have higher Fe2+ content than Cont. +Fe root tips after 24 h of split-root culture treatment[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT1080 human fibrosarcoma cells
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Concentration:0.2-12.8 μM (with 10 μM erastin)
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Incubation Time:12 h
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Result:Potently inhibited erastin-induced ferroptosis, with an IC50 value of 3.5 μM.
Significantly increased HT1080 cell viability compared to erastin-only treatment, achieving near-full rescue at higher concentrations.
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Cell Line:HT1080 human fibrosarcoma cells
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Concentration:0.2-12.8 μM (with 10 μM RSL3)
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Incubation Time:12 h
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Result:Demonstrated robust inhibitory activity against RSL3-induced ferroptosis, reducing cell death by more than 50%.
Significantly increased HT1080 cell viability compared to RSL3-only treatment.
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Cell Line:HT1080 human fibrosarcoma cells
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Concentration:12.8 μM (with lethal concentrations of FIN56 or FINO2)
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Incubation Time:12 h
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Result:Exhibited comparable inhibitory activity against FIN56- and FINO2-induced ferroptosis.
Effectively rescued HT1080 cell viability.
Chemical Information
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CAS No. 2711762-70-6
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Appearance Solid
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Molecular Weight 684.78
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Formula C38H44N4O8
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Color Off-white to pink
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SMILES
CC(C)(OC(N1CC[N+](C2=CC3=C(C4(C5=CC=C(C=C5O3)N6CCN(C(OC(C)(C)C)=O)CC6)OC(C7=C4C=CC=C7)=O)C=C2)(CC1)[O-])=O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Hirayama T, et al. -oxide-based and trioxolane-based fluorescent probes for monitoring labile iron and labile heme: an update. Journal of clinical biochemistry and nutrition. 2026 Jan;78(1):6-13. [Content Brief]
[2]. Kawai K, et al. Inhibition of ferroptosis by -oxide-based fluorescent probes selective oxidation of ferrous ions. Chemical science. 2025 Jun 25;16(25):11240-11245. [Content Brief]
[3]. Hirayama T, et al. High-Throughput Screening for the Discovery of Iron Homeostasis Modulators Using an Extremely Sensitive Fluorescent Probe. ACS sensors. 2020 Sep 25;5(9):2950-2958. [Content Brief]
[4]. Tabata R, et al. Systemic Regulation of Iron Acquisition by Arabidopsis in Environments with Heterogeneous Iron Distributions. Plant & cell physiology. 2022 Jun 15;63(6):842-854. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
RhoNox-4 Related Classifications
HY-D3116 Related Classifications
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