Cat. No.: HY-113134

25-Hydroxycholesterol

Name: 25-Hydroxycholesterol
Brand: MedChemExpress
SKU: HY-113134
Price: 55.00 USD
Availability: InStock
Rating: 5 (31 reviews)

(Synonyms: 25-OHC)

31 Cited Publications

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Based on 31 publication(s) in Google Scholar

25-Hydroxycholesterol (25-OHC) is a metabolite of cholesterol that is produced and secreted by macrophages in response to Toll-like receptor (TLR) activation. 25-hydroxycholesterol is a potent (EC₅₀≈65 nM) and selective suppressor of IgA production by B cells.

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Pureté : 99.65%
CAS No.: 2140-46-7
Formule: C₂₇H₄₆O₂
Masse moléculaire:402.65

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

25-Hydroxycholesterol Chemical Structure

CAS No.: 2140-46-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (31)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

Human Endogenous Metabolite

Voir tous les produits spécifiques à Isoform Endogenous Metabolite :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO-K1	IC₅₀	0.3 μM Compound: 25-hydroxycholesterol	Inhibition of SREBP2 activation expressed in CHO-K1 cells co-transfected with pSRE-Luc plasmid assessed as inhibition of luciferase expression after 20 hrs by luciferase reporter gene assay Inhibition of SREBP2 activation expressed in CHO-K1 cells co-transfected with pSRE-Luc plasmid assessed as inhibition of luciferase expression after 20 hrs by luciferase reporter gene assay	[PMID: 21561152]
HEK293	EC₅₀	2.3 μM Compound: 11, 25-HC	Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis	[PMID: 23830695]
HEK293	EC₅₀	3.3 μM Compound: 1, 25HC	Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy	[PMID: 24928400]
HEK293	EC₅₀	>10000 nM Compound: 39	Positive allosteric modulation of recombinant human GluN1/GluN2B receptor stably expressed in HEK293 cells assessed as increase in glycine/L-glutamate-induced channel current at -70 mV holding potential by IonWork two-electrode voltage clamp method Positive allosteric modulation of recombinant human GluN1/GluN2B receptor stably expressed in HEK293 cells assessed as increase in glycine/L-glutamate-induced channel current at -70 mV holding potential by IonWork two-electrode voltage clamp method	[PMID: 31390523]
HEK293	EC₅₀	>10000 nM Compound: 39	Positive allosteric modulation of recombinant human GluN1/GluN2A receptor stably expressed in HEK293 cells assessed as increase in glycine/L-glutamate-induced channel current at -70 mV holding potential by IonWork two-electrode voltage clamp method Positive allosteric modulation of recombinant human GluN1/GluN2A receptor stably expressed in HEK293 cells assessed as increase in glycine/L-glutamate-induced channel current at -70 mV holding potential by IonWork two-electrode voltage clamp method	[PMID: 31390523]
HEK293	EC₅₀	0.63 μM Compound: 25HC	Induction of human HMGCR-dCat-ELuc degradation expressed in HEK293 cells assessed as reduction in luciferase activity after 4 hrs by luciferase reporter assay Induction of human HMGCR-dCat-ELuc degradation expressed in HEK293 cells assessed as reduction in luciferase activity after 4 hrs by luciferase reporter assay	[PMID: 31902650]
HepG2	IC₅₀	0.3 μM Compound: 25-hydroxy-cholesterol	Tested for its inhibitory activity against HMG-CoA reductase transcription in HepG2 cells Tested for its inhibitory activity against HMG-CoA reductase transcription in HepG2 cells	[PMID: 8057282]
Vero	IC₅₀	>128 μM Compound: 7	Cytotoxicity against african green monkey Vero cells by tetrazolium dye reduction assay Cytotoxicity against african green monkey Vero cells by tetrazolium dye reduction assay	[PMID: 24084159]

In Vitro

The synthesis of 25-hydroxycholesterol is catalyzed by the enzyme cholesterol 25-hydroxylase, which uses cholesterol and molecular oxygen as substrates and NADPH as a cofactor. 25-hydroxycholesterol is a potent bioactive lipid in the innate and adaptive immune systems^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .

Chemical Information

CAS No. 2140-46-7
Appearance Solid
Masse moléculaire 402.65
Formule C₂₇H₄₆O₂
Color White to off-white
SMILES

CC(C)(O)CCC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
Synonyms

25-OHC
Structure Classification
- Steroids
Initial Source
- Endogenous metabolite
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (31)

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Journal Impact Factor

Mol Cancer

GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis. [Abstract]

2025 May 26;24(1):151. PMID: 40414849
Cell Metab

PIDDosome-SCAP crosstalk controls high-fructose-diet-dependent transition from simple steatosis to steatohepatitis. [Abstract]

2022 Oct 4;34(10):1548-1560.e6. PMID: 36041455

25-Hydroxycholesterol purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Oct 4;34(10):1548-1560.e6. [Abstract]

HEK293 cells were transfected with the indicated plasmids and supplemented with 25-Hydroxycholesterol (25-HC, 1 μg/ml) for 16 h. Indicated proteins were IB analyzed.
Nat Aging

Comparative analysis of senolytic drugs reveals mitochondrial determinants of efficacy and resistance. [Abstract]

2026 Feb;6(2):316-328. PMID: 41611832
Nat Commun

Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma. [Abstract]

2025 Jan 31;16(1):1217. PMID: 39890772

25-Hydroxycholesterol purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1217. [Abstract]

25-Hydroxycholesterol (1 µg/mL; 48 h). BMDM were incubated with B16F10-OVA VEGF-C tumor-conditioned medium (TCM), treated or not with 25-HC for 48 h, and analyzed by Q-PCR for PPAR-γ mRNA levels.

25-Hydroxycholesterol purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1217. [Abstract]

25-Hydroxycholesterol (1 µg/mL; 48 h). BMDMs were incubated with tumor-conditioned medium (TCM), treated or not with 25-HC, Troglitazone, or the combination of 25-HC and Troglitazone for 48 h. BMDM phenotype was analyzed by flow cytometry.
Mol Cell

FAM120A couples SREBP-dependent transcription and splicing of lipogenesis enzymes downstream of mTORC1. [Abstract]

2023 Aug 17;83(16):3010-3026.e8. PMID: 37595559

25-Hydroxycholesterol purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Aug 17;83(16):3010-3026.e8. [Abstract]

Co-IP analysis of LAM cell serum starved overnight (to induce SREBP cleavage) and treated with SREBP cleavage inhibitor (25-Hydroxycholesterol, 25-HC, 10 µM), or SRPK inhibitor (SRPKIN-1, 5 µM) for 4 h.
Phytomedicine

Disruption of cholesterol homeostasis triggers NLRP3-cGAS-STING axis-dependent hepatic fibrosis and honokiol intervention effects. [Abstract]

2025 Jul 25:143:156904. PMID: 40449452
Phytomedicine

Alisol B regulates AMPK/mTOR/SREBPs via directly targeting VDAC1 to alleviate hyperlipidemia. [Abstract]

2024 Jun:128:155313. PMID: 38520833
Nano Today

2025 Oct.
Dev Cell

Tumor-intrinsic FDFT1 determines coordinated macrophage anti-tumor immunity. [Abstract]

2026 Apr 29:S1534-5807(26)00150-4. PMID: 42061405
Cell Chem Biol

Transient hydroxycholesterol treatment restrains TCR signaling to promote long-term immunity. [Abstract]

2024 May 16;31(5):920-931.e6. PMID: 38759618
J Ethnopharmacol

Zexie Tang targeting FKBP38/mTOR/SREBPs pathway improves hyperlipidemia. [Abstract]

2022 May 23:290:115101. PMID: 35151834
J Agric Food Chem

Genistein Ameliorates Rifampicin-Undermined Hepatic Cholesterol Efflux via the CH25H-LXRα-ABCA Pathway. [Abstract]

2025 Dec 29. PMID: 41459645
Life Sci

Cordycepin reprogramming lipid metabolism to block metastasis and EMT via ERO1A/mTOR/SREBP1 axis in cholangiocarcinoma. [Abstract]

2023 Aug 15:327:121698. PMID: 37080351
Anal Chim Acta

2026 Jun 16;1418:345855.
Int Immunopharmacol

25-Hydroxycholesterol mitigates hepatic ischemia reperfusion injury via mediating mitophagy. [Abstract]

2021 Jul:96:107643. PMID: 33878616
Chem Biol Interact

25-Hydroxycholesterol induces oxidative stress, leading to apoptosis and ferroptosis in extravillous trophoblasts. [Abstract]

2024 Nov 1:403:111214. PMID: 39197811

25-Hydroxycholesterol purchased from MedChemExpress. Usage Cited in: Chem Biol Interact. 2024 Nov 1:403:111214. [Abstract]

25-Hydroxycholesterol (0-10 µM; 24-72 h). 25HC inhibits short-term proliferation in Swan 71 cells. The cells were exposed to the indicated concentrations of 25HC for 24, 48, and 72 h. Cell viability was measured using a WST assay.
Org Chem Front

15 Feb 2022.
Microbiol Spectr

25-Hydroxycholesterol Mediates Cholesterol Metabolism to Restrict Porcine Deltacoronavirus Infection via Suppression of Transforming Growth Factor β1. [Abstract]

2022 Dec 21;10(6):e0219822. PMID: 36314946
Microbiol Spectr

Two Cholesterol Recognition Amino Acid Consensus Motifs of GP64 with Uncleaved Signal Peptide Are Required for Bombyx mori Nucleopolyhedrovirus Infection. [Abstract]

2021 Dec 22;9(3):e0172521. PMID: 34937190
Vet Res

The cholesterol metabolite 25-hydroxycholesterol suppresses porcine deltacoronavirus via lipophagy inhibition and mTORC1 modulation. [Abstract]

2025 Jan 31;56(1):23. PMID: 39891192
Viruses

Screening of Botanical Drugs against SARS-CoV-2 Entry Reveals Novel Therapeutic Agents to Treat COVID-19. [Abstract]

2022 Feb 8;14(2):353. PMID: 35215943
Vet Res

CRISPR/Cas9-based generation of a recombinant double-reporter pseudorabies virus and its characterization in vitro and in vivo. [Abstract]

2021 Jun 26;52(1):95. PMID: 34174954
Viruses

Broad-Spectrum Host-Based Antivirals Targeting the Interferon and Lipogenesis Pathways as Potential Treatment Options for the Pandemic Coronavirus Disease 2019 (COVID-19). [Abstract]

2020 Jun 10;12(6):628. PMID: 32532085
J Med Virol

Bergamottin, A Natural Bioactive Compound, Inhibits Dabie Bandavirus Infection In Vitro and In Vivo. [Abstract]

2025 Jul;97(7):e70495. PMID: 40673712
Arch Biochem Biophys

Selective mitochondrial damage and dysfunction in cholesterol-exposed neuronal cells: Role of mitochondrial lipid peroxidation. [Abstract]

2026 Jun:780:110790. PMID: 41825807
Life Sci Alliance

Necrotic debris and STING exert therapeutically relevant effects on tumor cholesterol homeostasis. [Abstract]

2022 Jan 4;5(3):e202101256. PMID: 34983824
Vet Microbiol

Pseudorabies virus EP0 recruits TECPR2 and CK2 to promote COPII accumulation and viral egress. [Abstract]

2026 Jun 17:320:111115. PMID: 42322707
Vet Microbiol

The activation of liver X receptors in Madin-Darby bovine kidney cells and mice restricts infection by bovine viral diarrhea virus. [Abstract]

2024 Jan:288:109948. PMID: 38113573
Biochem Biophys Res Commun

Combinatorial screening of a panel of FDA-approved drugs identifies several candidates with anti-Ebola activities. [Abstract]

2020 Feb 19;522(4):862-868. PMID: 31806372
bioRxiv

Med14 phosphorylation shapes genomic response to GLP-1 Agonist. [Abstract]

2025 Jun 23:2025.06.17.660196. PMID: 40667025
medRxiv

OSBPL11 is an African-specific locus associated with 25-hydroxyvitamin D concentrations and cardiometabolic health. [Abstract]

2025 May 31:2025.05.27.25328359. PMID: 40492082

Solvant et solubilité

In Vitro:

Ethanol : 14.29 mg/mL (35.49 mM; Need ultrasonic)

DMSO : 5 mg/mL (12.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4835 mL

12.4177 mL

24.8355 mL

5 mM

0.4967 mL

2.4835 mL

4.9671 mL

10 mM

0.2484 mL

1.2418 mL

2.4835 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 0.5 mg/mL (1.24 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 0.5 mg/mL (1.24 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (284 KB)
SDS (422 KB)
- English - EN (422 KB)
- Français - FR (422 KB)
- Deutsch - DE (422 KB)
- Norwegian - NO (422 KB)
- Español - ES (422 KB)
- Swedish - SV (422 KB)
- Italian - IT (422 KB)
- Korean - KR (422 KB)
- Portuguese - PT (422 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. McDonald JG, et al. Editorial: 25-Hydroxycholesterol: a new life in immunology. J Leukoc Biol. 2010 Dec;88(6):1071-2. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / Ethanol	1 mM	2.4835 mL	12.4177 mL	24.8355 mL	62.0887 mL
	5 mM	0.4967 mL	2.4835 mL	4.9671 mL	12.4177 mL
	10 mM	0.2484 mL	1.2418 mL	2.4835 mL	6.2089 mL
Ethanol	15 mM	0.1656 mL	0.8278 mL	1.6557 mL	4.1392 mL
	20 mM	0.1242 mL	0.6209 mL	1.2418 mL	3.1044 mL
	25 mM	0.0993 mL	0.4967 mL	0.9934 mL	2.4835 mL
	30 mM	0.0828 mL	0.4139 mL	0.8278 mL	2.0696 mL

Bioz

Keywords

25-Hydroxycholesterol

31 Cited Publications

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25-Hydroxycholesterol

Publications Citing Use of MedChemExpress (MCE) 25-Hydroxycholesterol

Voir tous les produits spécifiques à Isoform Endogenous Metabolite