5-Hydroxyoxindole
Based on 1 Customer Validation
5-Hydroxyoxindole is an endogenous compound and a Uric acid (HY-B2130) analog. 5-Hydroxyoxindole selectively inhibits rat MAO-A. 5-Hydroxyoxindole scavenges DPPH radicals, inhibits lipid peroxidation, suppresses hydrogen peroxide-induced intracellular oxidative stress, and reduces ERK activity. 5-Hydroxyoxindole can be used in research on leukemia and neuroblastoma.
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- Pureté : 95.09%
- CAS No.: 3416-18-0
- Formule: C8H7NO2
- Masse moléculaire:149.15
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
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Human Endogenous Metabolite |
MAO-A |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| N1E-115 | EC50 |
21.2 μM
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EC50 for inhibition of N1E-115 cell proliferation assessed by [3H]thymidine incorporation after 20 hrs exposure and 4 hrs labeling.
EC50 for inhibition of N1E-115 cell proliferation assessed by [3H]thymidine incorporation after 20 hrs exposure and 4 hrs labeling.
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11006132 |
| BALB/3T3 | EC50 |
15.0 μM
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EC50 for inhibition of BALB/c3T3 cell proliferation assessed by [3H]thymidine incorporation after 20 hrs exposure and 4 hrs labeling.
EC50 for inhibition of BALB/c3T3 cell proliferation assessed by [3H]thymidine incorporation after 20 hrs exposure and 4 hrs labeling.
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11006132 |
| PC-12 | EC50 |
54.7 μM
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EC50 for inhibition of PC12 cell proliferation assessed by [3H]thymidine incorporation after 20 hrs exposure and 4 hrs labeling.
EC50 for inhibition of PC12 cell proliferation assessed by [3H]thymidine incorporation after 20 hrs exposure and 4 hrs labeling.
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11006132 |
| HL-60 | EC50 |
58.2 μM
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EC50 for inhibition of HL60 cell proliferation assessed by [3H]thymidine incorporation after 20 hrs exposure and 4 hrs labeling.
EC50 for inhibition of HL60 cell proliferation assessed by [3H]thymidine incorporation after 20 hrs exposure and 4 hrs labeling.
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11006132 |
| NIH3T3 | EC50 |
34.8 μM
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EC50 for inhibition of NIH 3T3 cell proliferation assessed by [3H]thymidine incorporation after 20 hrs exposure and 4 hrs labeling.
EC50 for inhibition of NIH 3T3 cell proliferation assessed by [3H]thymidine incorporation after 20 hrs exposure and 4 hrs labeling.
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11006132 |
In Vitro
5-Hydroxyoxindole (20 min) inhibits lipid peroxidation in rat liver microsome/tert-butyl hydroperoxide assays[4].
5-Hydroxyoxindole (100 μM; 60 min) shows no significant iron-induced pro-oxidant effect in the cell-free formaldehyde generation assay[4].
5-hydroxyoxindole inhibits tert-butyl hydroperoxide-induced lipid peroxidation in rat liver microsomes, with an IC50 of 300 μM[5].
5-Hydroxyoxindole (0-100 µM; 5 min) inhibits ERK activity in N1E-115 lysates, reaching 20% inhibition at 60 µM[3].
5-Hydroxyoxindole (0-100 µM; 5 min) strongly reduces ERK2 phosphorylation in N1E-115 cells at 10 µM, whereas ERK1 phosphorylation is only minimally affected[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:N1E-115, BALB/c3T3, BBC, PC12, HL60, and NIH 3T3 cells
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Concentration:Concentration-response for EC50 determination
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Incubation Time:20 h (exposure); 4 h (thymidine incorporation)
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Result:EC50 values were 21.2 µM in N1E-115, 15.0 µM in BALB/c3T3, and 19.4 µM in BBC cells.
Additional EC50 values were 54.7 µM in PC12, 58.2 µM in HL60, and 34.8 µM in NIH 3T3 cells.
At 0.1 mM, cell proliferation was reduced by 80% compared with controls.
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Cell Line:N1E-115 cells
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Concentration:0, 10, 30, 60, and 100 µM
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Incubation Time:5 min (pre-exposure); 5 min (FCS stimulation)
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Result:At 10 µM, ERK2 phosphorylation was strongly reduced.
In contrast, ERK1 phosphorylation was little affected.
Chemical Information
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CAS No. 3416-18-0
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Appearance Solid
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Masse moléculaire 149.15
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Formule C8H7NO2
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Color Light brown to brown
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SMILES
O=C1NC2=C(C=C(O)C=C2)C1
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (670.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Pureté et documentation
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Fiche technique (279 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.7047 mL | 33.5233 mL | 67.0466 mL | 167.6165 mL |
| 5 mM | 1.3409 mL | 6.7047 mL | 13.4093 mL | 33.5233 mL | |
| 10 mM | 0.6705 mL | 3.3523 mL | 6.7047 mL | 16.7617 mL | |
| 15 mM | 0.4470 mL | 2.2349 mL | 4.4698 mL | 11.1744 mL | |
| 20 mM | 0.3352 mL | 1.6762 mL | 3.3523 mL | 8.3808 mL | |
| 25 mM | 0.2682 mL | 1.3409 mL | 2.6819 mL | 6.7047 mL | |
| 30 mM | 0.2235 mL | 1.1174 mL | 2.2349 mL | 5.5872 mL | |
| 40 mM | 0.1676 mL | 0.8381 mL | 1.6762 mL | 4.1904 mL | |
| 50 mM | 0.1341 mL | 0.6705 mL | 1.3409 mL | 3.3523 mL | |
| 60 mM | 0.1117 mL | 0.5587 mL | 1.1174 mL | 2.7936 mL | |
| 80 mM | 0.0838 mL | 0.4190 mL | 0.8381 mL | 2.0952 mL | |
| 100 mM | 0.0670 mL | 0.3352 mL | 0.6705 mL | 1.6762 mL |
5-Hydroxyoxindole Related Classifications
HY-W001542 Related Classifications
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Keywords
- 5-Hydroxyoxindole
- 3416-18-0
- Free Radical Scavengers
- Drug Derivative
- Endogenous Metabolite
- Monoamine Oxidase
- ERK
- Drug derivative
- MAO
- Extracellular signal regulated kinases
- DPPH radical scavenging
- ERK activity
- ERK2 phosphorylation
- apoptosis
- intracellular oxidative stress
- leukemia
- lipid peroxidation
- monoamine oxidase A
- monoamine oxidase B
- neuroblastoma
- Inhibitor
- inhibitor
- inhibit