Cat. No.: HY-B0511

Biotin

(Synonyms: Vitamin B7; Vitamin H; D-Biotin)

28 Cited Publications

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Based on 28 publication(s) in Google Scholar

Biotin, vitamin B7 and serves as a coenzyme for five carboxylases in humans, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids.

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Pureté: 99.16%
CAS No.: 58-85-5
Formule: C₁₀H₁₆N₂O₃S
Masse moléculaire:244.31

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Biotin Chemical Structure

CAS No.: 58-85-5

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (28)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Voir tous les produits spécifiques à Isoform Endogenous Metabolite :

Cellular Effect

Cell Line	Type	Value	Description	References
ES-2	IC₅₀	>100 μM Compound: Biotin	Antiproliferative activity against human ES2 cells Antiproliferative activity against human ES2 cells	[PMID: 38716896]
HEK-293T	IC₅₀	>100 μM Compound: Biotin	Antiproliferative activity against HEK293T Antiproliferative activity against HEK293T	[PMID: 38716896]
OVCAR-3	IC₅₀	>100 μM Compound: Biotin	Antiproliferative activity against human OVCAR-3 cells Antiproliferative activity against human OVCAR-3 cells	[PMID: 38716896]

In Vitro

Biotin exhibits higher affinity on breast cancer (T47D) cells over normal mammary epithelial (MCF-12A) cells, with K_ms of 9.24 μM and 53.1 μM, respecetively^[4].
Biotin (0.09-100 μM; 0-70 min) is dose-dependently uptake by T47D cells with V_max of 27.34 pmol/mg protein/min^[4].
Biotin (1-1000 nM; 24 h) exerts function in the recovery of 7β-OHC (50 µM)-induced cell death and reduces cell adhesion^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[5]

Cell Line:

Murine oligodendrocyte 158N cells
Concentration:

1, 10, 100, 1000 nM
Incubation Time:

24 hours
Result:

Showed cytoprotective effects and prevents 7β-hydroxycholesterol-induced disruption of redox status.
Improved attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration.

In Vivo

Biotin (15 mg/kg/d; p.o.; 12 d) improves nephrotoxicity in streptozotocin-induced (150 mg/kg; i.p.) diabetic mice^[6].
Biotin (0.012 mg/kg/d; p.o.; 70 d) deficiency impaires the immune function of the head kidney, spleen and skin in fish with inadequate administration dose^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Streptozotocin-induced Diabetes type 1 in male Swiss albino mice (25±2 g)^[6]
Dosage:

15 mg/kg/d
Administration:

Oral gavage; 12 days
Result:

Improved histopathological results, including distorted glomeruli, inflammatory cells, and giant macrophages, and diminished acroline reaction of oxidative damage.

Animal Model:

Grass carp (117±0.5 g)^[7]
Dosage:

0.012, 0.110, 0.214, 0.311, 0.427, and 0.518 mg/kg
Administration:

Oral gavage; 70 days
Result:

Reduced the activities of lysozyme (LZ) and acid phosphatase (ACP), decreased the contents of complement 3 (C3), C4 and immunoglobulin M (IgM).
Reduced the mRNA levels of anti-microbial substances.
Increased the mRNA levels of pro-inflammatory cytokines, tumour necrosis factor partially and reduced anti-inflammatory IL-4/13A, IL-10, IL-11 and TGF-β1 mRNA levels partially in association with target of rapamycin (TOR) signaling.

Chemical Information

CAS No. 58-85-5
Appearance Solid
Masse moléculaire 244.31
Formule C₁₀H₁₆N₂O₃S
Color White to off-white
SMILES

O=C(O)CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O
Synonyms

Vitamin B7; Vitamin H; D-Biotin
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Plants
- other families
- Microorganisms
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (28)

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Mol Cell

CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity. [Abstract]

2023 Dec 7;83(23):4370-4385.e9. PMID: 38016475
Acta Pharm Sin B

Obesity-driven oleoylcarnitine accumulation in tumor microenvironment promotes breast cancer metastasis-like phenotype. [Abstract]

2025 Apr;15(4):1974-1990. PMID: 40486851

Biotin purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Apr;15(4):1974-1990. [Abstract]

Immunoblotting was performed to detect the presence of ADCY10 following co-incubation with cell lysate and subsequent precipitation of bound protein using streptavidin magnetic beads with either Bio-OLCarn or Biotin (20 mmol/L) for 12 h at 4 °C with continuous rotation.

Biotin purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Apr;15(4):1974-1990. [Abstract]

Fluorescence intensity represents the colocalization of ADCY10 and Bio-OLCarn or Biotin (20 mmol/L) in HCC1806 cells overnight at 4 ℃; green: ADCY10, red: Bio-OLCarn.
Autophagy

Regorafenib enhances anti-PDCD1/PD-1 therapeutic efficacy in colorectal cancer by promoting SQSTM1/p62-mediated CD274/PD-L1 degradation. [Abstract]

2026 Feb 17:1-18. PMID: 41655134
Adv Sci (Weinh)

Hepatocyte BDNF Acts as a Novel Immune Checkpoint to Restrain TLR4-Mediated Acute Hepatitis. [Abstract]

2026 Mar 25:e21164. PMID: 41881032
J Clin Invest

Targeting ubiquitin-independent proteasome with small molecule increases susceptibility in pan-KRAS-mutant cancer models. [Abstract]

2025 Mar 17;135(6):e185278. PMID: 40091835

Biotin purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2025 Mar 17;135(6):e185278. [Abstract]

Biotin pull-down assay. SW620 cell protein lysates were incubated with biotin, biotin-RLY01 (B-RLY01; 10 μM), B-RLY01 (10 μM) + 100 μM RLY01 (10× molar excess), B-RLY01 (10 μM) + 200 μM RLY01 (20× molar excess) at room temperature for 3 hours by rotation.
Theranostics

Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription. [Abstract]

2020 May 22;10(15):6839-6853. PMID: 32550907

Biotin purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 May 22;10(15):6839-6853. [Abstract]

Immunoblot with anti-NCOR2, anti-ACACA, anti-FASN, anti-CAD, and anti-α-Tubulin antibodies of protein precipitated by streptavidin beads from MDA-MB-231 cell lysates in the presence of B-Emodin or Biotin (10 µM) for 5 h at 4 ℃ with rotation.
J Exp Clin Cancer Res

Dual CDK and MEK Inhibition potentiates CD8+ T cell-mediated antitumor immunity by inducing pyroptotic cell death in high-mutational head and neck cancer. [Abstract]

2025 Nov 6;44(1):300. PMID: 41194215
Sci Adv

2025 Dec 19;11(51).
Cell Discov

Suppressing protein damage response to overcome multidrug resistance in cancer therapy. [Abstract]

2025 Sep 30;11(1):80. PMID: 41027888

Biotin purchased from MedChemExpress. Usage Cited in: Cell Discov. 2025 Sep 30;11(1):80. [Abstract]

Biotin (50 μM, 3 h). MDA-MB-231 cells were treated with indicated drugs for 3 h, and biotin was pulled down. Interacting proteins were stained with Coomassie brilliant blue and K48 polyUB was detected by western blotting
MedComm (2020)

Caffeic acid, but not ferulic acid, inhibits macrophage pyroptosis by directly blocking gasdermin D activation. [Abstract]

2023 Apr 20;4(3):e255. PMID: 37090118
Pharmacol Res

Brusatol has therapeutic efficacy in non-small cell lung cancer by targeting Skp1 to inhibit cancer growth and metastasis. [Abstract]

2022 Feb:176:106059. PMID: 34998973
Int J Biol Sci

Tryptophan-Restricted Intermittent Diet Alleviates Estrogen Deficiency-Induced Osteoporosis via Regulating Coupling Effects of "Gut-Bone" Axis. [Abstract]

2026 Apr 23;22(9):4784-4805. PMID: 42157925
Cancer Biol Med

An isoflavone derivative potently inhibits the angiogenesis and progression of triple-negative breast cancer by targeting the MTA2/SerRS/VEGFA pathway. [Abstract]

2020 Aug 15;17(3):693-706. PMID: 32944400
ACS Environ Au

Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]

2025 Aug 5;5(6):573-582. PMID: 41277996
Cell Rep

CRISPR screening identifies PRMT1 as a key pro-ferroptotic gene via a two-layer regulatory mechanism. [Abstract]

2024 Aug 22;43(9):114662. PMID: 39178116
Cell Rep

PRRC2B modulates oligodendrocyte progenitor cell development and myelination by stabilizing Sox2 mRNA. [Abstract]

2024 Mar 18;43(3):113930. PMID: 38507412
Anal Chem

Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]

2025 Jun 3;97(21):11099-11109. PMID: 40401576
Drug Deliv Transl Res

Leveraging quantum chemical properties in transfer learning for predicting blood-brain barrier permeability of drugs. [Abstract]

2025 Oct 29. PMID: 41160380
Commun Biol

Pantothenic acid ameliorates hepatic fibrosis by targeting IGFBP6 to regulate the TGF-β/SMADs pathway. [Abstract]

2025 Jul 29;8(1):1127. PMID: 40730679
Int J Mol Sci

Isorhamnetin Regulates Programmed Death Ligand-1 Expression by Suppressing the EGFR-STAT3 Signaling Pathway in Canine Mammary Tumors. [Abstract]

2024 Jan 4;25(1):670. PMID: 38203840
Cell Rep Methods

Graft-seq precisely maps RNA modifications via site-specific chemical grafting strategy. [Abstract]

2025 Jul 21;5(7):101103. PMID: 40645188
Mol Nutr Food Res

Salvianolic Acid A Protects Against Angiotensin II-Induced Hypertensive Kidney Disease by Targeting TLR4 and Inhibiting TLR4/Myd88/PI3K/AKT/NF-κB Pathway. [Abstract]

2026 Jan;70(1):e70371. PMID: 41543314
ACS Chem Biol

Selective Degradation of GSPT1 by Cereblon Modulators Identified via a Focused Combinatorial Library. [Abstract]

2020 Oct 16;15(10):2722-2730. PMID: 32865967
PLoS One

Proteomic screening of TMEM43 binding partners identifies VDAC leading to mitochondrial dysfunction. [Abstract]

2025 Dec 18;20(12):e0339129. PMID: 41411330
Cell Biochem Biophys

Amentoflavone Induces Ferroptosis to Alleviate Proliferation, Migration, Invasion and Inflammation in Rheumatoid Arthritis Fibroblast-like Synoviocytes by Inhibiting PIN1. [Abstract]

2025 Mar;83(1):1299-1312. PMID: 39354278
Res Sq

2026 Apr 22.
Res Sq

2024 Nov 26.
bioRxiv

2024 July 19.

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (409.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.0932 mL

20.4658 mL

40.9316 mL

5 mM

0.8186 mL

4.0932 mL

8.1863 mL

10 mM

0.4093 mL

2.0466 mL

4.0932 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 1.96 mg/mL (8.02 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (281 KB)
SDS (396 KB)
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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
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MS (2659 KB)
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IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Zempleni, et al. Biotin biochemistry and human requirements. J Nutr Biochem, 1999. 10(3): p. 128-38. [Content Brief]

[2]. Mock, D.M.. Biotin status: which are valid indicators and how do we know? J Nutr, 1999. 129(2S Suppl): p. 498S-503S. [Content Brief]

[3]. Zempleni J, et al. Biotin. Biofactors. 2009 Jan-Feb;35(1):36-46. [Content Brief]

[4]. Vadlapudi AD, et al. Biotin uptake by T47D breast cancer cells: functional and molecular evidence of sodium-dependent multivitamin transporter (SMVT). Int J Pharm. 2013 Jan 30;441(1-2):535-43. [Content Brief]

[5]. Sghaier R, et al. Biotin attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration and 7β-hydroxycholesterol-induced cell death in 158N murine oligodendrocytes. Free Radic Res. 2019 May;53(5):535-561. [Content Brief]

[6]. Aldahmash BA, et al. Biotin amelioration of nephrotoxicity in streptozotocin-induced diabetic mice. Saudi J Biol Sci. 2015 Sep;22(5):564-9. [Content Brief]

[7]. He P, et al. Dietary biotin deficiency decreased growth performance and impaired the immune function of the head kidney, spleen and skin in on-growing grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2020 Feb;97:216-234. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.0932 mL	20.4658 mL	40.9316 mL	102.3290 mL
	5 mM	0.8186 mL	4.0932 mL	8.1863 mL	20.4658 mL
	10 mM	0.4093 mL	2.0466 mL	4.0932 mL	10.2329 mL
	15 mM	0.2729 mL	1.3644 mL	2.7288 mL	6.8219 mL
	20 mM	0.2047 mL	1.0233 mL	2.0466 mL	5.1165 mL
	25 mM	0.1637 mL	0.8186 mL	1.6373 mL	4.0932 mL
	30 mM	0.1364 mL	0.6822 mL	1.3644 mL	3.4110 mL
	40 mM	0.1023 mL	0.5116 mL	1.0233 mL	2.5582 mL
	50 mM	0.0819 mL	0.4093 mL	0.8186 mL	2.0466 mL
	60 mM	0.0682 mL	0.3411 mL	0.6822 mL	1.7055 mL
	80 mM	0.0512 mL	0.2558 mL	0.5116 mL	1.2791 mL
	100 mM	0.0409 mL	0.2047 mL	0.4093 mL	1.0233 mL

Keywords

Biotin

28 Cited Publications

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Biotin

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Publications Citing Use of MedChemExpress (MCE) Biotin

Voir tous les produits spécifiques à Isoform Endogenous Metabolite

Activité biologique

Chemical Information

Publications (28)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Biotin Related Classifications

HY-B0511 Related Classifications