country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-N10313

Copy Link Print Information

Dehydrobruceine B

Dehydrobruceine B, a quassinoid, can be isolated from Brucea javanica. Dehydrobruceine B shows a synergistic effect with Cisplatin (HY-17394) to induce apoptosis via mitochondrial method. Dehydrobruceine B increases apoptosis-inducing factor (AIF) and Bax expression and suppresses Keap1-Nrf2.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

CAS No.: 53730-90-8
Formule: C₂₃H₂₆O₁₁
Masse moléculaire:478.45

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (309 KB)

Dehydrobruceine B Chemical Structure

CAS No.: 53730-90-8

Stock

Quantité

Amount: USD 0.00

* Veuillez sélectionner la quantité avant d'ajouter des articles.

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

^[1]

Bax

Bcl-xL

Bcl-2

Voir tous les produits spécifiques à Isoform Bcl-2 Family :

In Vitro

Dehydrobruceine B (1 μM; 48 h) and Cisplatin (3-18 μM; 48 h) combination synergizes the cytotoxic and apoptotic effects in A549 cells^[1].
Dehydrobruceine B (1 μM; 24 h) induces excessive generation of intracellular ROS in Cisplatin (3 μM, 6 μM; 24 h)-treated A549 cells^[1].
Dehydrobruceine B (1 μM; 24 h) increases depolarization of mitochondrial membrane potential (MMP) and translocation of cytochrome c in Cisplatin (3 μM, 6 μM; 24 h)-treated A549 cells^[1].
Dehydrobruceine B (1 μM; 24 h) enhances the change of anti-apoptotic and pro-apoptotic protein levels in A549 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

A549 cells
Concentration:

1 μM; with or without Cisplatin
Incubation Time:

48 hours
Result:

Upregulated the protein level of Bax, while downregulated the levels of Bcl-2 and Bcl-xL.
Enhanced caspase activation and PARP cleavage.

Apoptosis Analysis^[1]

Cell Line:

A549 cells
Concentration:

1 μM; with or without Cisplatin
Incubation Time:

48 hours
Result:

Inhibited cell viability with 9 μM and 18 μM Cisplatin, respectively.
Induced cell apoptosis with 3 μM and 6 μM Cisplatin, respectively.

Immunofluorescence^[1]

Cell Line:

A549 cells
Concentration:

1 μM; with or without 3 μM and 6 μM Cisplatin, respectively
Incubation Time:

24 hours
Result:

Resulted apoptosis-inducing factor (AIF) translocated from cytosol into nucleus dramatically in the co-treatment condition.

Related Protocols :

Chemical Information

CAS No. 53730-90-8
Masse moléculaire 478.45
Formule C₂₃H₂₆O₁₁
SMILES

COC([C@]12[C@@]3([H])[C@@]4(CO2)[C@@]([C@H]([C@@H]1O)O)([H])[C@@]5(C(C[C@@]4([H])OC([C@@H]3OC(C)=O)=O)=C(C(C(O)=C5)=O)C)C)=O
Structure Classification
- Others
Initial Source
- Microorganism
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (309 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Huang Z, et al. Dehydrobruceine B enhances the cisplatin-induced cytotoxicity through regulation of the mitochondrial apoptotic pathway in lung cancer A549 cells. Biomed Pharmacother. 2017 May;89:623-631. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

Obtenir un devis En stock

Other size

Obtenir un devis