Cat. No.: HY-N1372A

Fangchinoline

11 Cited Publications

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Based on 11 publication(s) in Google Scholar

Fangchinoline is isolated from Stephania tetrandra with extensive biological activities, such as enhancing immunity, anti-inflammatory sterilization and anti-atherosclerosis. Fangchinoline, a novel HIV-1 inhibitor, inhibits HIV-1 replication by impairing gp160 proteolytic processing. Fangchinoline targets Focal adhesion kinase (FAK) and suppresses FAK-mediated signaling pathway in tumor cells which highly expressed FAK. Fangchinoline induces apoptosis and adaptive autophagy in bladder cancer.

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Pureté: 99.90%
CAS No.: 436-77-1
Formule: C₃₇H₄₀N₂O₆
Masse moléculaire:608.72

Stockage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Fangchinoline Chemical Structure

CAS No.: 436-77-1

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (11)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Fangchinoline is isolated from Stephania tetrandra with extensive biological activities, such as enhancing immunity, anti-inflammatory sterilization and anti-atherosclerosis. Fangchinoline, a novel HIV-1 inhibitor, inhibits HIV-1 replication by impairing gp160 proteolytic processing^[1]. Fangchinoline targets Focal adhesion kinase (FAK) and suppresses FAK-mediated signaling pathway in tumor cells which highly expressed FAK^[2]. Fangchinoline induces apoptosis and adaptive autophagy in bladder cancer^[3].

IC₅₀ & Target

HIV-1

Voir tous les produits spécifiques à Isoform HIV :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>10 μM Compound: 14	Cytotoxicity against human A549 cells after 48 hrs by MTS assay Cytotoxicity against human A549 cells after 48 hrs by MTS assay	[PMID: 23621840]
ECa-109 cell line	IC₅₀	>10 μM Compound: 14	Cytotoxicity against human ECA109 cells after 48 hrs by MTT assay Cytotoxicity against human ECA109 cells after 48 hrs by MTT assay	[PMID: 23621840]
HEK-293T	CC₅₀	>1 μM Compound: 2; fan	Cytotoxicity against HEK293T cells assessed as reduction in cell viability by CellTiter-Glo assay Cytotoxicity against HEK293T cells assessed as reduction in cell viability by CellTiter-Glo assay	[PMID: 37043739]
HEL	IC₅₀	22.71 μM Compound: Fangchinoline	Cytotoxicity against human HEL cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HEL cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784186]
HepG2	IC₅₀	9.08 μM Compound: Fangchinoline	Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 28057423]
HL-60	IC₅₀	>10 μM Compound: 14	Cytotoxicity against human HL60 cells after 48 hrs by MTS assay Cytotoxicity against human HL60 cells after 48 hrs by MTS assay	[PMID: 23621840]
K562	IC₅₀	5.94 μM Compound: Fangchinoline	Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784186]
KB	ED₅₀	9210 nM Compound: 20	Cytotoxicity against human KB cells after 72 hrs by SRB assay Cytotoxicity against human KB cells after 72 hrs by SRB assay	[PMID: 9917283]
MCF7	IC₅₀	10.1 μM Compound: Fangchinoline	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 28057423]
MCF7	IC₅₀	17.41 μM Compound: Fangchinoline	Cytotoxicity against human MCF-7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MCF-7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784186]
MCF7	IC₅₀	5 μM Compound: 14	Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay	[PMID: 23621840]
MDA-MB-231	IC₅₀	58.61 μM Compound: Fangchinoline	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784186]
SMMC-7721	IC₅₀	>10 μM Compound: 14	Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay	[PMID: 23621840]
SW480	IC₅₀	>10 μM Compound: 14	Cytotoxicity against human SW480 cells after 48 hrs by MTS assay Cytotoxicity against human SW480 cells after 48 hrs by MTS assay	[PMID: 23621840]
U-937	IC₅₀	>50 μM Compound: 15, NSC 77036	Cytotoxicity against human U937 cells after 24 hrs by alamar blue assay Cytotoxicity against human U937 cells after 24 hrs by alamar blue assay	[PMID: 22766217]

In Vitro

Fangchinoline (2.5-40 μM; 24-96 hours) inhibits both T24 and 5637 cells in dose-dependent manner, the IC₅₀ values of Fangchinoline in T24 cells are 19.0 μM (24 h), 12.0 μM (48 h) and 7.57 μM (72 h), and 11.9 μM (24 h), 9.92 μM (48 h) and 7.13 μM (72 h) in 5637 cells^[1].
Fangchinoline (5 μM; 24 hours) induces a significant increase in the LC3-II/LC3-I ratio and a decrease in p62 in both T24 and 5637 cells, and causes a significant increase in the cleavage of caspase-3^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

T24 and 5637 cells
Concentration:

2.5 µM; 5 µM; 10 µM; 20 µM; 30 µM; 40 µM
Incubation Time:

24 hours; 48 hours; 96 hours
Result:

Inhibited both T24 and 5637 cells proliferation.

Western Blot Analysis^[3]

Cell Line:

T24 and 5637 cells
Concentration:

5 µM
Incubation Time:

24 hours
Result:

Incresed LC3-II/LC3-I ratio and the cleavage of caspase-3.

Chemical Information

CAS No. 436-77-1
Appearance Solid
Masse moléculaire 608.72
Formule C₃₇H₄₀N₂O₆
Color White to off-white
SMILES

OC1=C(OC2=CC([C@]3([H])CC(C=C4)=CC=C4O5)=C(CCN3C)C=C2OC)C([C@](N6C)([H])CC7=CC=C(OC)C5=C7)=C(CC6)C=C1OC
Structure Classification
- Alkaloids
- Isoquinoline Alkaloids
- Phenols
- Monophenols
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (11)

All Cell Proliferation/Viability AssayFlow CytometryIFWBCell Migration/Invasion Assay

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Journal Impact Factor

J Adv Res

Thioredoxin-interacting protein-activated intracellular potassium deprivation mediates the anti-tumour effect of a novel histone acetylation inhibitor HL23, a fangchinoline derivative, in human hepatocellular carcinoma. [Abstract]

2023 Sep:51:181-196. PMID: 36351536
Cancer Lett

Fangchinoline inhibits non-small cell lung cancer metastasis by reversing epithelial-mesenchymal transition and suppressing the cytosolic ROS-related Akt-mTOR signaling pathway. [Abstract]

2022 Sep 1:543:215783. PMID: 35700820

Fangchinoline purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2022 Sep 1:543:215783. [Abstract]

CCK-8 examination of the viability of Fangchinoline (Fan)-treated NSCLC cells.

Fangchinoline purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2022 Sep 1:543:215783. [Abstract]

NSCLC cells were stained with CFDA-SE and treated with Fangchinoline (Fan) (1.25, 2.5, 5 μM) for 24 h. The mean fluorescence intensity was detected by flow cytometry in the FITC channel.

Fangchinoline purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2022 Sep 1:543:215783. [Abstract]

Cell viability was determined by the EdU assay treated with Fangchinoline (Fan) (1.25, 2.5, 5 μM).

Fangchinoline purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2022 Sep 1:543:215783. [Abstract]

EMT-related biomarker detection revealed that Fangchinoline (Fan) (1.25, 2.5, 5 μM) significantly inhibited EMT dose-dependent.

Fangchinoline purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2022 Sep 1:543:215783. [Abstract]

The effect of Fangchinoline (Fan) (1.25, 2.5, 5 μM, 24, 48 h) on cell migration and invasion was assessed with the wound healing assay.
Int J Biol Sci

Fangchinoline Activates cGAS-STING to Promote Antitumor Immunity without Pathological Inflammation. [Abstract]

2026 May 18;22(10):5444-5458. PMID: 42212320
Phytomedicine

Fangchinoline suppresses nasopharyngeal carcinoma progression by inhibiting SQLE to regulate the PI3K/AKT pathway dysregulation. [Abstract]

2025 May:140:156484. PMID: 40090046
Virol J

Bisbenzylisoquinoline alkaloids inhibit influenza virus replication by disrupting endosomal acidification. [Abstract]

2025 Jun 4;22(1):181. PMID: 40468427
Toxicol Appl Pharmacol

Fangchinoline alleviates the progression of osteoarthritis through the nuclear factor kappa B signaling pathway. [Abstract]

2025 Jan 31:117241. PMID: 39894170
Front Oncol

Fangchinoline Inhibits Human Esophageal Cancer by Transactivating ATF4 to Trigger Both Noxa-Dependent Intrinsic and DR5-Dependent Extrinsic Apoptosis. [Abstract]

2021 Jun 14:11:666549. PMID: 34195076
Integr Cancer Ther

Unveiling the Anticancer Potential and Molecular Mechanisms of Fangchinoline Against Cholangiocarcinoma Using FTIR Microspectroscopy, In Vitro and In Silico Approaches. [Abstract]

2025 Jan-Dec:24:15347354251396513. PMID: 41261767
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Biomed Pharmacother

Fangchinoline suppresses melanoma metastasis by inducing senescence of circulating tumor cells. [Abstract]

2025 Nov 14:193:118770. PMID: 41242135
SSRN

2023 Feb 14.

Solvant et solubilité

In Vitro:

DMSO : 50 mg/mL (82.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6428 mL

8.2140 mL

16.4279 mL

5 mM

0.3286 mL

1.6428 mL

3.2856 mL

10 mM

0.1643 mL

0.8214 mL

1.6428 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (3.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (3.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (285 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Wan Z, et al. Fangchinoline inhibits human immunodeficiency virus type 1 replication by interfering with gp160 proteolytic processing. PLoS One. 2012;7(6):e39225. [Content Brief]

[2]. Guo B, et al. Fangchinoline as a kinase inhibitor targets FAK and suppresses FAK-mediated signaling pathway in A549. J Drug Target. 2015 Apr;23(3):266-74. [Content Brief]

[3]. Fan B, et al. Fangchinoline Induces Apoptosis, Autophagy and Energetic Impairment in Bladder Cancer. Cell Physiol Biochem. 2017;43(3):1003-1011. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6428 mL	8.2140 mL	16.4279 mL	41.0698 mL
	5 mM	0.3286 mL	1.6428 mL	3.2856 mL	8.2140 mL
	10 mM	0.1643 mL	0.8214 mL	1.6428 mL	4.1070 mL
	15 mM	0.1095 mL	0.5476 mL	1.0952 mL	2.7380 mL
	20 mM	0.0821 mL	0.4107 mL	0.8214 mL	2.0535 mL
	25 mM	0.0657 mL	0.3286 mL	0.6571 mL	1.6428 mL
	30 mM	0.0548 mL	0.2738 mL	0.5476 mL	1.3690 mL
	40 mM	0.0411 mL	0.2053 mL	0.4107 mL	1.0267 mL
	50 mM	0.0329 mL	0.1643 mL	0.3286 mL	0.8214 mL
	60 mM	0.0274 mL	0.1369 mL	0.2738 mL	0.6845 mL
	80 mM	0.0205 mL	0.1027 mL	0.2053 mL	0.5134 mL

Keywords

Fangchinoline

11 Cited Publications

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Fangchinoline

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Publications Citing Use of MedChemExpress (MCE) Fangchinoline

Voir tous les produits spécifiques à Isoform HIV

Activité biologique

Chemical Information

Publications (11)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Fangchinoline Related Classifications

HY-N1372A Related Classifications