NSAH
Based on 1 Customer Validation
NSAH is a reversible and competitive nonnucleoside ribonucleotide reductase (RR) inhibitor, with cell-free IC50 of 32 μM and cell-based IC50 of ~250 nM, respectively.
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- Pureté : 98.45%
- CAS No.: 1099592-35-4
- Formule: C18H14N2O3
- Masse moléculaire:306.32
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
225 nM
Compound: E-3a
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Growth inhibition of human HCT116 cells after 72 hrs by Hoechst 33258 dye based fluorescence assay
Growth inhibition of human HCT116 cells after 72 hrs by Hoechst 33258 dye based fluorescence assay
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[PMID: 29253340] |
| MDA-MB-231 | IC50 |
0.347 μM
Compound: E-3a
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Growth inhibition of human MDA-MB-231 cells treated with 4 pmol RRM1 siRNA after 5 days by Hoechst 33258 dye based fluorescence assay
Growth inhibition of human MDA-MB-231 cells treated with 4 pmol RRM1 siRNA after 5 days by Hoechst 33258 dye based fluorescence assay
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[PMID: 29253340] |
| MDA-MB-231 | IC50 |
1.43 μM
Compound: E-3a
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Growth inhibition of human MDA-MB-231 cells treated with 2 pmol RRM1 siRNA at 0.1 to 10 uM after 5 days by Hoechst 33258 dye based fluorescence assay
Growth inhibition of human MDA-MB-231 cells treated with 2 pmol RRM1 siRNA at 0.1 to 10 uM after 5 days by Hoechst 33258 dye based fluorescence assay
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[PMID: 29253340] |
| MDA-MB-231 | IC50 |
1.49 μM
Compound: E-3a
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Growth inhibition of human MDA-MB-231 cells after 5 days by Hoechst 33258 dye based fluorescence assay
Growth inhibition of human MDA-MB-231 cells after 5 days by Hoechst 33258 dye based fluorescence assay
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[PMID: 29253340] |
| MDA-MB-231 | IC50 |
1.94 μM
Compound: E-3a
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Growth inhibition of human MDA-MB-231 cells treated with 4 pmol scrambled siRNA after 5 days by Hoechst 33258 dye based fluorescence assay
Growth inhibition of human MDA-MB-231 cells treated with 4 pmol scrambled siRNA after 5 days by Hoechst 33258 dye based fluorescence assay
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[PMID: 29253340] |
| MDA-MB-231 | IC50 |
300 nM
Compound: E-3a
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Growth inhibition of human MDA-MB-231 cells after 72 hrs by Hoechst 33258 dye based fluorescence assay
Growth inhibition of human MDA-MB-231 cells after 72 hrs by Hoechst 33258 dye based fluorescence assay
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[PMID: 29253340] |
In Vitro
NSAH depresses dGTP and dATP levels in the dNTP pool causing S-phase arrest, providing evidence for RR inhibition in cells[1].
NSAH (0-10 μM, 2, 6, 24, or 72 h) exhibits potent anti-tumor activity in 3 cancer cell lines[1].
NSAH blocks S-phase progression well to the extent and timing of the decreases in dATP and dGTP[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MDA-231, HCT116 and Panc1 cancer cell lines.
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Concentration:0-10 μM.
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Incubation Time:2, 6, 24, or 72 h.
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Result:Resulted in IC50 values ranging from 220 to 500 nM.
Chemical Information
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CAS No. 1099592-35-4
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Appearance Solid
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Masse moléculaire 306.32
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Formule C18H14N2O3
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Color Light yellow to yellow
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SMILES
O=C(C1=CC=CC=C1O)N/N=C/C2=C3C=CC=CC3=CC=C2O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 62.5 mg/mL (204.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureté et documentation
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Fiche technique (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2646 mL | 16.3228 mL | 32.6456 mL | 81.6140 mL |
| 5 mM | 0.6529 mL | 3.2646 mL | 6.5291 mL | 16.3228 mL | |
| 10 mM | 0.3265 mL | 1.6323 mL | 3.2646 mL | 8.1614 mL | |
| 15 mM | 0.2176 mL | 1.0882 mL | 2.1764 mL | 5.4409 mL | |
| 20 mM | 0.1632 mL | 0.8161 mL | 1.6323 mL | 4.0807 mL | |
| 25 mM | 0.1306 mL | 0.6529 mL | 1.3058 mL | 3.2646 mL | |
| 30 mM | 0.1088 mL | 0.5441 mL | 1.0882 mL | 2.7205 mL | |
| 40 mM | 0.0816 mL | 0.4081 mL | 0.8161 mL | 2.0403 mL | |
| 50 mM | 0.0653 mL | 0.3265 mL | 0.6529 mL | 1.6323 mL | |
| 60 mM | 0.0544 mL | 0.2720 mL | 0.5441 mL | 1.3602 mL | |
| 80 mM | 0.0408 mL | 0.2040 mL | 0.4081 mL | 1.0202 mL | |
| 100 mM | 0.0326 mL | 0.1632 mL | 0.3265 mL | 0.8161 mL |
NSAH Related Classifications
HY-114503 Related Classifications
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