SLD1121
Based on 1 Customer Validation
SLD1121 is an agonist of the Wnt/β-catenin signaling pathway that enhances Wnt signaling by targeting LRP6 (Kd: 4.52-7.49 nM). SLD1121 interacts with the intracellular domain of LRP6, stabilizes LRP6, promotes its nuclear translocation, and facilitates its binding to β-catenin, TCF4 or LEF1 in the nucleus, thereby inducing the expression of Wnt-regulated genes and stem cell-related genes. SLD1121 induces the transition of hair follicles from telogen to anagen in the mouse hair growth cycle and promotes hair growth in mice. SLD1121 is applicable to hair loss-related research.
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- Pureté : 99.98%
- CAS No.: 2759011-75-9
- Formule: C26H17N5O
- Masse moléculaire:415.45
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Stockage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
SLD1121 (0-2 μM; 24 h) activates the LRP6-mediated Wnt/β-catenin signaling pathway in HEK293T cells, with an EC50 of approximately 0.21 μM[1].
SLD1121 (1-2 μM; 24 h) activates the Wnt/β-catenin signaling pathway in HaCaT cells[1].
SLD1121 (0.25-2 μM; 4-24 h) dose- and time-dependently increases the levels of total LRP6 protein, phosphorylated LRP6 protein, as well as ABC and β-catenin in HEK293T and HaCaT cells, without altering the mRNA expression level of LRP6[1].
SLD1121 (2 μM; 24 h) directly interacts with LRP6 in HEK293T cells and increases the thermal stability of both endogenous and exogenous LRP6[1].
SLD1121 (0-2 μM; 12-24 h) promotes the nuclear translocation of LRP6 and increases the nuclear β-catenin level in HaCaT cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HaCaT cells
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Concentration:1 and 2 μM
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Incubation Time:24 h
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Result:Upregulated the mRNA expression of Wnt target genes Axin2, LEF1, Snail1, OCT4, Twist1, Sox2, and CD44.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6J (7-week-old female, depilated to induce synchronized hair follicle telogen phase)[1]
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Dosage:2%
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Administration:topical; daily; 17 days
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Result:Induced gray skin (indicating early anagen transition) on day 9.
Showed significantly higher hair coat recovery rates compared to vehicle controls on days 11, 15, and 17.
Increased regrown hair weight significantly relative to vehicle controls on day 17.
Increased anagen-phase hair follicle numbers and dermal thickness compared to vehicle-treated mice (remained in telogen) via histological H&E staining.
Upregulated protein levels of phosphorylated LRP6, total LRP6, ABC, β-catenin, cytokeratin 15, and cytokeratin 17 significantly via western blotting.
Increased expression of LRP6, β-catenin, ABC, cytokeratin 15, cytokeratin 17, and proliferative marker Ki67 in skin tissues confirmed via immunohistochemical staining.
Increased expression of Sox2 and Snail in hair follicles shown via immunofluorescence staining.
Chemical Information
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CAS No. 2759011-75-9
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Appearance Solid
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Masse moléculaire 415.45
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Formule C26H17N5O
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Color Off-white to pale purple
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SMILES
O=C(NC1=CC=CC=C1)C(C=C2)=CC=C2C3=NC4=C5C(N=CC=C5)=C6C(C=CC=N6)=C4N3
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvant et solubilité
In Vitro:
DMSO : 14.29 mg/mL (34.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.43 mg/mL (3.44 mM); Suspended solution
This protocol yields a suspended solution of ≥ 1.43 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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Cytoplasmic-Nuclear Fractionated Protein Extraction
Cytoplasmic-nuclear fractionated protein extraction separates soluble cytoplasmic proteins from nuclear-enriched proteins by mild plasma-membrane permeabilization, differential centrifugation, washing of nuclei, and extraction of nuclear proteins for downstream immunoblotting or related molecular analysis. The readout is the relative abundance of a protein in cytoplasmic and nuclear fractions, commonly assessed by western blotting together with compartment markers such as tubulin or pyruvate kinase for cytoplasm and lamin, nucleoporin, hnRNP, H2AX, or Lamin B for nuclear fractions.
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
Pureté et documentation
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Fiche technique (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4070 mL | 12.0351 mL | 24.0703 mL | 60.1757 mL |
| 5 mM | 0.4814 mL | 2.4070 mL | 4.8141 mL | 12.0351 mL | |
| 10 mM | 0.2407 mL | 1.2035 mL | 2.4070 mL | 6.0176 mL | |
| 15 mM | 0.1605 mL | 0.8023 mL | 1.6047 mL | 4.0117 mL | |
| 20 mM | 0.1204 mL | 0.6018 mL | 1.2035 mL | 3.0088 mL | |
| 25 mM | 0.0963 mL | 0.4814 mL | 0.9628 mL | 2.4070 mL | |
| 30 mM | 0.0802 mL | 0.4012 mL | 0.8023 mL | 2.0059 mL |
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