Art. -Nr.: HY-19381

WAY-151932

(Synonyms: VNA-932; WAY-VNA 932)

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WAY-151932 is a vasopressin V₂-receptor agonist with IC₅₀ of 80.3 nM and 778 nM in human-V₂ binding and V_1a binding assay.

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Reinheit: 99.87%
CAS. Nr.: 220460-92-4
Formel: C₂₃H₁₉ClN₄O
Molecular Weight:402.88

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

WAY-151932 Chemical Structure

CAS. Nr.: 220460-92-4

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

WAY-151932 is a vasopressin V₂-receptor agonist with IC₅₀ of 80.3 nM and 778 nM in human-V₂ binding and V_1a binding assay.

IC₅₀ & Target

IC50: 80.3 nM (human-V2 binding), 778 nM (human-V1a binding)^[1]

Alle Vasopressin Receptor Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	125 nM Compound: 3, WAY-VNA-932	Activity at human oxytocin receptor expressed in CHO cells by NFAT-luciferase gene reporter assay Activity at human oxytocin receptor expressed in CHO cells by NFAT-luciferase gene reporter assay	[PMID: 20104850]
CHO	EC₅₀	39.9 nM Compound: 3, WAY-VNA-932	Activity at human vasopressin V2 receptor expressed in CHO cells by NFAT-luciferase gene reporter assay Activity at human vasopressin V2 receptor expressed in CHO cells by NFAT-luciferase gene reporter assay	[PMID: 20104850]
CHO	EC₅₀	465 nM Compound: 3, WAY-VNA-932	Activity at human vasopressin V1a receptor expressed in CHO cells by NFAT-luciferase gene reporter assay Activity at human vasopressin V1a receptor expressed in CHO cells by NFAT-luciferase gene reporter assay	[PMID: 20104850]
CHO	IC₅₀	353 nM Compound: 1	Displacement of [3H]oxytocin from human oxytocin receptor expressed in CHO cells Displacement of [3H]oxytocin from human oxytocin receptor expressed in CHO cells	[PMID: 17855087]
CHO	IC₅₀	778 nM Compound: 1	Displacement of [3H]manning ligand from human vasopressin V1a receptor expressed in CHO cells Displacement of [3H]manning ligand from human vasopressin V1a receptor expressed in CHO cells	[PMID: 17855087]
CHO	IC₅₀	778 nM Compound: VNA-932	Displacement of [3H]AVP from human V1a receptor expressed in CHO cells Displacement of [3H]AVP from human V1a receptor expressed in CHO cells	[PMID: 16297621]
HEK293	EC₅₀	<1 nM Compound: 2, VAN-932	Agonist activity at human vasopressin V2 receptor expressed in HEK293 cells assessed as increase in intracellular cAMP level by CRE-luciferase reporter gene assay Agonist activity at human vasopressin V2 receptor expressed in HEK293 cells assessed as increase in intracellular cAMP level by CRE-luciferase reporter gene assay	[PMID: 19053774]

In Vitro

WAY-151932 (VNA-932) stimulates cAMP formation in LV2 cells expressing the hV₂ receptors in a dose-dependent manner with an EC₅₀ of 0.74±0.07 nM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Oral administration of WAY-151932 (VNA-932) to water-loaded conscious rats produces a dose-dependent decrease in urine volume (ED₅₀=0.14 mg/kg, 2.5% starch in water vehicle) and a corresponding increase in osmolality without altering the urine electrolyte excretion profile^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 220460-92-4
Appearance Solid
Molecular Weight 402.88
Formel C₂₃H₁₉ClN₄O
Color White to off-white
SMILES

O=C(C1=CC=C(N2N=C(C)C=C2)C=C1Cl)N3CC4=CC=CN4CC5=CC=CC=C53
Synonyms

VNA-932; WAY-VNA 932
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 200 mg/mL (496.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4821 mL

12.4106 mL

24.8213 mL

5 mM

0.4964 mL

2.4821 mL

4.9643 mL

10 mM

0.2482 mL

1.2411 mL

2.4821 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (12.41 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (12.41 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (12.41 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Molinari AJ, et al. Identification and synthesis of major metabolites of Vasopressin V2-receptor agonist WAY-151932, and antagonist, Lixivaptan. Bioorg Med Chem Lett. 2007 Nov 1;17(21):5796-800. [Content Brief]

[2]. Failli AA, et al. Pyridobenzodiazepines: a novel class of orally active, vasopressin V2 receptor selective agonists. Bioorg Med Chem Lett. 2006 Feb 15;16(4):954-9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4821 mL	12.4106 mL	24.8213 mL	62.0532 mL
	5 mM	0.4964 mL	2.4821 mL	4.9643 mL	12.4106 mL
	10 mM	0.2482 mL	1.2411 mL	2.4821 mL	6.2053 mL
	15 mM	0.1655 mL	0.8274 mL	1.6548 mL	4.1369 mL
	20 mM	0.1241 mL	0.6205 mL	1.2411 mL	3.1027 mL
	25 mM	0.0993 mL	0.4964 mL	0.9929 mL	2.4821 mL
	30 mM	0.0827 mL	0.4137 mL	0.8274 mL	2.0684 mL
	40 mM	0.0621 mL	0.3103 mL	0.6205 mL	1.5513 mL
	50 mM	0.0496 mL	0.2482 mL	0.4964 mL	1.2411 mL
	60 mM	0.0414 mL	0.2068 mL	0.4137 mL	1.0342 mL
	80 mM	0.0310 mL	0.1551 mL	0.3103 mL	0.7757 mL
	100 mM	0.0248 mL	0.1241 mL	0.2482 mL	0.6205 mL

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WAY-151932

GPCR/G Protein Compound Library

Clinical Compound Library

Endocrinology Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Clinical Compound Library Plus

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Alle Vasopressin Receptor Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

WAY-151932

GPCR/G Protein Compound Library

Clinical Compound Library

Endocrinology Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Clinical Compound Library Plus

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Alle Vasopressin Receptor Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

WAY-151932 Related Classifications

HY-19381 Related Classifications