Art. -Nr.: HY-101086

Acetylcholine iodide

(Synonyms: ACh iodide)

28 Cited Publications

Kundenbewertung

Based on 28 publication(s) in Google Scholar

Acetylcholine iodide is a muscarinic receptor modulator. Acetylcholine iodide specifically binds to muscarinic receptors, inhibits sodium absorption, and induces chloride secretion. Acetylcholine iodide changes intestinal ion transport, enhances intestinal secretory function, induces or maintains mammary gland development and lactation. Acetylcholine iodide can be used for intestinal ion transport regulation and mammary gland physiological function research.

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Reinheit: 98.59%
CAS. Nr.: 2260-50-6
Formel: C₇H₁₆INO₂
Molecular Weight:273.11

Speicherung:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Acetylcholine iodide Chemical Structure

CAS. Nr.: 2260-50-6

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (28)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Human Endogenous Metabolite

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Alle mAChR Isoform-spezifische Produkte anzeigen :

In Vitro

Acetylcholine iodide (25 mM; added to the serosal fluid of the rat proximal colonic eversion sac) increases the potential difference in the rat proximal colon^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Acetylcholine iodide (3 μg; i.v.) increases the electrical potential difference in the proximal colon of rats^[2].
Acetylcholine iodide (25 mg/kg; subcutaneous injection; twice a day; 15 days) induces mammary ductal growth in some virgin rats^[3].
Acetylcholine iodide (25 mg/kg; subcutaneous injection; twice a day; 5 days) induces lobular-acinar growth and secretion in the mammary glands of virgin rats pretreated with estrogen^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female albino rats (Carworth, weighing 175 - 200 g, age unspecified) for studying mammary growth and lactation induction; Female albino rats (Carworth, postparturient, litter removed on 4th day after parturition) for studying maintenance of mammary structure and secretion^[3].
Dosage:

25 mg/kg, 50 mg/kg (dissolved in an unspecified solvent for subcutaneous injection)
Administration:

Subcutaneous injection, twice daily for 15 days (for induction in virgin rats), twice daily for 5 days after 10 days of estradiol pretreatment (for induction in estrogen-primed virgin rats), twice daily for 10 days (for maintenance in lactating rats after litter removal)
Result:

Induced duct growth in some cases without estradiol priming, and lobule-alveolar growth and secretion with estradiol priming in virgin rats.
Retarded mammary involution and maintained lactation in lactating rats after litter removal.

Chemical Information

CAS. Nr. 2260-50-6
Appearance Solid
Molecular Weight 273.11
Formel C₇H₁₆INO₂
Color White to off-white
SMILES

O=C(OCC[N+](C)(C)C)C.[I-]
Synonyms

ACh iodide
Structure Classification
- Others
Initial Source
- Animals
A transmitter substance of nerve impulses within the central and peripheral nervous systems
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (28)

All IFCell Proliferation/Viability AssayFlow CytometryIn Vivo Efficacy Study

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Journal Impact Factor

Cancer Cell

Long-range cholinergic input promotes glioblastoma progression. [Abstract]

2025 Aug 12:S1535-6108(25)00330-7. PMID: 40829591
Cell

Sympathetic-epithelial crosstalk governs tissue-resident memory T cell immunosurveillance in the skin. [Abstract]

2026 Mar 5;189(5):1323-1340.e25. PMID: 41616781
Cell Res

Light sensing enhances thermotolerance and competitive fitness via serotonergic signaling in an eyeless organism. [Abstract]

2026 Apr;36(4):286-299. PMID: 41764323
Trends Biotechnol

Programmable embedded bioprinting for one-step manufacturing of arterial models with customized contractile and metabolic functions. [Abstract]

2025 Apr;43(4):918-945. PMID: 39779422
Redox Biol

Myeloid ACE2 protects against septic hypotension and vascular dysfunction through Ang-(1-7)-Mas-mediated macrophage polarization. [Abstract]

2024 Feb:69:103004. PMID: 38141575
MedComm (2020)

Mas-Related G Protein-Coupled Receptor Member D Sustains Hypertension. [Abstract]

2026 Mar 28;7(4):e70706. PMID: 41930327
Phytomedicine

Andrographolide ameliorates vascular injury in hypertension by suppressing EndMT via Sp1-Notch1 signaling. [Abstract]

2026 Jan 17:153:157846. PMID: 41653618
J Hazard Mater

β-ionone inhibits the grazing of Daphnia sinensis by reducing the activity of acetylcholinesterase. [Abstract]

2024 Sep 5:479:135690. PMID: 39255669
J Hazard Mater

β-cyclocitral, a novel AChE inhibitor, contributes to the defense of Microcystis aeruginosa against Daphnia grazing. [Abstract]

2024 Mar 5:465:133248. PMID: 38147752
Acta Pharmacol Sin

Huperzine A attenuates epileptic seizures via enhancing dCA1-projecting septal cholinergic transmission. [Abstract]

2025 Mar 26. PMID: 40140526

Acetylcholine iodide purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2025 Mar 26. [Abstract]

Acetylcholine (ACh, 20 μM; 250 nL, locally injected into the dorsal CA1 (dCA1)). AAV-EF1α-FLEX-taCasp3 virus was injected in the medial septum (MS) and the cannula was implanted in the dCA1 for Hup A (0.1 -1 mg/kg; i.p.) intervention. Effects of Hup A on the development of seizure stage (Left) and ADDs (Right) during kindling acquisition, in the condition of AAV-taCasp3-mediated apoptosis of the ChAT⁺ neurons in MS, n=9.
Proc Natl Acad Sci U S A

Cholinergic macrophages promote the resolution of peritoneal inflammation. [Abstract]

2024 Jul 2;121(27):e2402143121. PMID: 38923993

Acetylcholine iodide purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121. [Abstract]

The impact of ACh (Acetylcholine, 1 μM; 24 h) on the uptake of latex beads-rabbit IgG-FITC complex (green) in murine BMDMs (red; Upper) or human MDMs (red; Lower) was assessed. The data are presented as the percentage of latex beads-rabbit IgG-FITC-positive cells relative to the total cell count. (Scale bar, 100 μm.)

Acetylcholine iodide purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121. [Abstract]

BMDMs from ChAT-GFP mice were treated with Pam3, or in combination with ACh (Acetylcholine, 10 μM), for 24 h and subsequently evaluated for GFP expression.

Acetylcholine iodide purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Jul 2;121(27):e2402143121. [Abstract]

ACh (Acetylcholine, 5 mg/kg; i.p., once daily for 3 days). The number of apoptotic neutrophils was assessed by flow cytometry at day 3 post-Pam3 stimulation in Chat^fl/fl and Chat^fl/flLyz2^cre mice.
Curr Biol

Molecular pathways for learning in the single-cell Stentor coeruleus. [Abstract]

2026 May 4;36(9):2367-2381.e9. PMID: 42025171
J Ethnopharmacol

Mailuoning oral liquid ameliorates vasculitis in thromboangiitis obliterans rats via inactivating cGAS-STING-IRF3 and TLR4-MAPKs/NF-κB signaling pathways. [Abstract]

2024 Aug 22:118707. PMID: 39181282
Mol Metab

Vagal control of the brain-esophagus axis ameliorates stress-induced esophageal motility dysfunction in male mice. [Abstract]

2026 May 26:102384. PMID: 42203107
Mol Metab

Deletion of Ascl1 in pancreatic β-cells improves insulin secretion, promotes parasympathetic innervation, and attenuates dedifferentiation during metabolic stress. [Abstract]

2023 Dec:78:101811. PMID: 37769990
Cells

Single-Cell Hypertrophy Promotes Contractile Function of Cultured Human Airway Smooth Muscle Cells via Piezo1 and YAP Auto-Regulation. [Abstract]

2024 Oct 14;13(20):1697. PMID: 39451215
Int J Mol Sci

A Clozapine-Responsive GPCR-Based Gene Switch for Pharmacological Control of Gene Expression in Mammalian Cells and In Vivo. [Abstract]

2026 Apr 9;27(8):3381. PMID: 42074026
Biochim Biophys Acta Mol Basis Dis

Dietary apigenin ameliorates obesity-related hypertension through TRPV4-dependent vasorelaxation and TRPV4-independent adiponectin secretion. [Abstract]

2024 Aug 30:167488. PMID: 39218272
ACS Pharmacol Transl Sci

Critical Residues in the Gβ Subunit Mediate Selective Recruitment of the Gαq Heterotrimer to Activated G Protein-Coupled Receptors. [Abstract]

2025 Jun 6;8(7):2048-2060. PMID: 40672669
Mol Cell Endocrinol

Vagotomy suppresses food intake by increasing GLP-1 secretion via the M3 AChR-AMPKα pathway in mice. [Abstract]

2025 Jan 21:599:112464. PMID: 39848433
BMC Pulm Med

Opposing responses of the rat pulmonary artery and vein to phenylephrine and other agents in vitro. [Abstract]

2021 Jun 5;21(1):189. PMID: 34090386
Front Cardiovasc Med

Estrogen Protects Vasomotor Functions in Rats During Catecholamine Stress. [Abstract]

2021 Jun 16:8:679240. PMID: 34222374
Clin Exp Pharmacol Physiol

Indole-3-Propionic Acid Improves Vascular Function in High-Fat Diet-Induced Obese Mice via eNOS. [Abstract]

2025 Nov;52(11):e70081. PMID: 41057979
Turk J Med Sci

Benzyl alcohol improves Ang II-induced vascular and renal injury. [Abstract]

2025 Feb 19;55(2):509-517. PMID: 40342326
UCSF

2025.
Res Sq

2025 Nov 12.
Biomed Pharmacother

Spinosad blocks CHRNA5 mediated EGFR signaling pathway activation to inhibit lung adenocarcinoma proliferation. [Abstract]

2024 Jul 12:177:117105. PMID: 39002438

Acetylcholine iodide purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Jul 12:177:117105. [Abstract]

The addition of exogenous Acetylcholine (1-10 μM) reversed the decline in H1975 cell viability induced by Spinosad (10 μM).
bioRxiv

2023 Oct 13.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (366.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 100 mg/mL (366.15 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6615 mL

18.3076 mL

36.6153 mL

5 mM

0.7323 mL

3.6615 mL

7.3231 mL

10 mM

0.3662 mL

1.8308 mL

3.6615 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (9.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (9.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (9.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
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- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Stavinoha WB, et al. The use of microwave heating to inactivate cholinesterase in the rat brain prior to analysis for acetylcholine. J Neurochem. 1973 Feb;20(2):361-71. [Content Brief]

[2]. Browning JG, et al. The role of acetylcholine in the regulation of ion transport by rat colon mucosa. J Physiol. 1977 Nov;272(3):737-54. [Content Brief]

[3]. MEITES J, et al. Induction and maintenance of mammary growth and lactation in rats with acetylcholine or epinephrine. Proc Soc Exp Biol Med. 1959 Apr;100(4):750-4. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	3.6615 mL	18.3076 mL	36.6153 mL	91.5382 mL
	5 mM	0.7323 mL	3.6615 mL	7.3231 mL	18.3076 mL
	10 mM	0.3662 mL	1.8308 mL	3.6615 mL	9.1538 mL
	15 mM	0.2441 mL	1.2205 mL	2.4410 mL	6.1025 mL
	20 mM	0.1831 mL	0.9154 mL	1.8308 mL	4.5769 mL
	25 mM	0.1465 mL	0.7323 mL	1.4646 mL	3.6615 mL
	30 mM	0.1221 mL	0.6103 mL	1.2205 mL	3.0513 mL
	40 mM	0.0915 mL	0.4577 mL	0.9154 mL	2.2885 mL
	50 mM	0.0732 mL	0.3662 mL	0.7323 mL	1.8308 mL
	60 mM	0.0610 mL	0.3051 mL	0.6103 mL	1.5256 mL
	80 mM	0.0458 mL	0.2288 mL	0.4577 mL	1.1442 mL
	100 mM	0.0366 mL	0.1831 mL	0.3662 mL	0.9154 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Acetylcholine iodide

28 Cited Publications

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Acetylcholine iodide

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Publications Citing Use of MedChemExpress (MCE) Acetylcholine iodide

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Alle mAChR Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (28)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Acetylcholine iodide Related Classifications

HY-101086 Related Classifications