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Art. -Nr.: HY-N10313

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Dehydrobruceine B

Dehydrobruceine B, a quassinoid, can be isolated from Brucea javanica. Dehydrobruceine B shows a synergistic effect with Cisplatin (HY-17394) to induce apoptosis via mitochondrial method. Dehydrobruceine B increases apoptosis-inducing factor (AIF) and Bax expression and suppresses Keap1-Nrf2.

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CAS. Nr.: 53730-90-8
Formel: C₂₃H₂₆O₁₁
Molecular Weight:478.45

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (309 KB)

Dehydrobruceine B Chemical Structure

CAS. Nr.: 53730-90-8

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

^[1]

Bax

Bcl-xL

Bcl-2

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen :

In Vitro

Dehydrobruceine B (1 μM; 48 h) and Cisplatin (3-18 μM; 48 h) combination synergizes the cytotoxic and apoptotic effects in A549 cells^[1].
Dehydrobruceine B (1 μM; 24 h) induces excessive generation of intracellular ROS in Cisplatin (3 μM, 6 μM; 24 h)-treated A549 cells^[1].
Dehydrobruceine B (1 μM; 24 h) increases depolarization of mitochondrial membrane potential (MMP) and translocation of cytochrome c in Cisplatin (3 μM, 6 μM; 24 h)-treated A549 cells^[1].
Dehydrobruceine B (1 μM; 24 h) enhances the change of anti-apoptotic and pro-apoptotic protein levels in A549 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

A549 cells
Concentration:

1 μM; with or without Cisplatin
Incubation Time:

48 hours
Result:

Upregulated the protein level of Bax, while downregulated the levels of Bcl-2 and Bcl-xL.
Enhanced caspase activation and PARP cleavage.

Apoptosis Analysis^[1]

Cell Line:

A549 cells
Concentration:

1 μM; with or without Cisplatin
Incubation Time:

48 hours
Result:

Inhibited cell viability with 9 μM and 18 μM Cisplatin, respectively.
Induced cell apoptosis with 3 μM and 6 μM Cisplatin, respectively.

Immunofluorescence^[1]

Cell Line:

A549 cells
Concentration:

1 μM; with or without 3 μM and 6 μM Cisplatin, respectively
Incubation Time:

24 hours
Result:

Resulted apoptosis-inducing factor (AIF) translocated from cytosol into nucleus dramatically in the co-treatment condition.

Chemical Information

CAS. Nr. 53730-90-8
Molecular Weight 478.45
Formel C₂₃H₂₆O₁₁
SMILES

COC([C@]12[C@@]3([H])[C@@]4(CO2)[C@@]([C@H]([C@@H]1O)O)([H])[C@@]5(C(C[C@@]4([H])OC([C@@H]3OC(C)=O)=O)=C(C(C(O)=C5)=O)C)C)=O
Structure Classification
- Others
Initial Source
- Microorganisms
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (309 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Huang Z, et al. Dehydrobruceine B enhances the cisplatin-induced cytotoxicity through regulation of the mitochondrial apoptotic pathway in lung cancer A549 cells. Biomed Pharmacother. 2017 May;89:623-631. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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