DJK-5

DJK-5 (10 μg/mL; 3-4 min) + 8.5% EDTA + 6% NaOCl irrigation protocol potently kills mono-species Enterococcus faecalis and multi-species oral biofilms in human dentinal tubules (51-60.5% efficacy), delays biofilm recovery to 16 weeks, and demonstrates superior killing efficacy during re-irrigation of recovered biofilms compared to non-peptide protocols^[1].
DJK-5 (18-24 h) exhibits planktonic antimicrobial activity against P. aeruginosa LESB58 (MIC 50 μg/mL in MHB, 100 μg/mL in DFG) and S. aureus USA300 LAC (MIC 25 μg/mL in MHB, 6.25 μg/mL in DFG), and shows synergistic activity with Colistin (HY-113678) against P. aeruginosa in MHB (FICI 0.5) and additive activity with Colistin against S. aureus in DFG (FICI 0.75)^[2].
DJK-5 (50 μg/mL; 24 h after 72 h biofilm growth) alone has no activity against mature monomicrobial P. aeruginosa LESB58 or S. aureus USA300 LAC biofilms in DFG, but when combined with 125 μg/mL Colistin, it reduces S. aureus USA300 LAC monomicrobial biofilm CFU by 0.5-log (5-fold)^[2].
DJK-5 (50 μg/mL; 24 h after 72 h biofilm growth) alone reduces S. aureus USA300 LAC CFU by 1.6-log (40-fold) in P. aeruginosa LESB58/S. aureus USA300 LAC co-biofilms in DFG, and when combined with either 125 μg/mL or 6.25 μg/mL Colistin, it exhibits synergistic activity, reducing P. aeruginosa CFU by up to ~3.3-log (2,140-fold) and S. aureus CFU by up to ~3.0-log (1,050-fold) compared to no treatment^[2].
DJK-5 (50 μg/mL; 24 h after 1 h SHX pre-incubation) activity against P. aeruginosa LESB58/S. aureus USA300 LAC co-biofilms in DFG is abolished by induction of the stringent stress response with 1 mM SHX, and the synergistic activity of DJK-5 combined with 125 μg/mL Colistin is eliminated^[2].
DJK-5 (50 μg/mL; 24 h after 72 h biofilm growth) alone reduces S. aureus USA300 LAC CFU in P. aeruginosa LESB58/S. aureus USA300 LAC co-biofilms in DFG, and when combined with 10 μg/mL Daptomycin (HY-B0108), it reduces P. aeruginosa CFU by ~1.5-log (30-fold) and exhibits synergistic activity against S. aureus, reducing CFU by 1.9-log (80-fold) compared to individual treatments^[2].
DJK-5 (40 μg/mL; 3 h after 72 h biofilm growth) induces morphological changes in P. aeruginosa LESB58 (shortened rods) and S. aureus USA300 LAC (wrinkled, reduced-area cells) in monomicrobial and co-biofilms in DFG, and when combined with Colistin, it increases membrane damage and cellular debris formation in both species^[2].
DJK-5 (50 μg/mL; 1 h after 72 h biofilm growth) alone increases membrane leakage from S. aureus USA300 LAC::pJB185sarAP1 by 25-fold in P. aeruginosa LESB58/S. aureus co-biofilms in DFG, and when combined with 125 μg/mL Colistin, it increases membrane leakage by 35-fold^[2].
DJK-5 (50 μg/mL; 6 h) potently inhibits P. aeruginosa PAO1 association and biofilm formation on plastic and 3-D A549 lung epithelial cell aggregates in serum-free medium, with stronger activity on plastic, and exerts modest inhibitory activity on 3-D A549 cell-associated bacteria in medium containing 10% FBS; combination with Tobramycin (HY-B0441) enhances its efficacy in both media conditions^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.