Iscalimab (WT IgG1)
Based on 1 Customer Validation
Iscalimab (WT IgG1) (CFZ-533) is a non-depleting IGg1 monoclonal antibody targeting CD40 (KD: 0.3 nM). Iscalimab (WT IgG1) can be used for research of Graves' hyperthyroidism and autoimmune diseases.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.64%
- CAS. Nr.: 2031153-61-2
- Molecular Weight:146.08 kDa
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Beschreibung
Isotype
Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
[1]|
CD40 0.3 nM (IC50) |
In Vitro
Iscalimab (WT IgG1) (0.01-1 μg/mL, overnight) blocks rCD154-induced TNF production by primary monocyte-derived dendritic cells (moDCs), with an IC50 of 0.04 μg/mL[3].
Iscalimab (WT IgG1) (3 days) inhibits rCD154-induced proliferation of PBMCs from humans, rhesus and cynomolgus animals with IC50s of 0.02, 0.03, and 0.01 μg/mL, respectively[3].
Iscalimab (WT IgG1) bind CD40 on B cells from humans, rhesus and cynomolgus animals with EC50 values of approximately 0.2 μg/mL[3].
Iscalimab (WT IgG1) (2 μg/mL, 3 h) is internalized by B cells in a CD40-dependent manner[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
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Cell Line:PBMCs from humans, rhesus and cynomolgus animals
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Concentration:0-1 μg/mL approximately
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Incubation Time:3 days
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Result:Inhibited rCD154-induced proliferation of PBMCs with IC50s of 0.02, 0.03, and 0.01 μg/mL, respectively.
In Vivo
Iscalimab (WT IgG1) (10 mg/kg, i.v.) completely inhibits T cell-dependent antibody response in Rhesus monkeys[3].
Iscalimab (WT IgG1) (30 mg/kg, i.v.) prolongs allograft survival in kidney transplant cynomolgus[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Kidney transplant cynomolgus[4]
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Dosage:30 mg/kg
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Administration:i.v.
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Result:Prolonged allograft survival.
Well-tolerated with no evidence of thromboembolic events or CD40 pathway activation.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Human IgG1 kappa
Anwendung
ELISA, FACS, Functional assay
Verified Bioactivity
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Immobilized CD40 Protein, Human can bind Iscalimab. The EC50 for this effect is 7.761 ng/mL. -
Flow Cytometry analysis of Raji cells labelling TNFRSF5/CD40 (red) with Iscalimab (anti-TNFRSF5/CD40) (HY-P99670). Goat Anti-Human IgG (Alexa Fluor 488) (HY-P83776) at a dilution of 1/1000 was used as the secondary antibody. Blue-Human IgG1 kappa (HY-P99001). Black-Unlabelled control, cells without incubation with primary antibody.
Chemical Information
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CAS. Nr. 2031153-61-2
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Appearance Liquid
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Molecular Weight 146.08 kDa
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Color Colorless to light yellow
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SMILES
[Iscalimab (WT IgG1)]
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Synonyms
CFZ-533 (WT IgG1); OM11-62MF (WT IgG1)
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Versand
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Inhibitory Antibodies User Guide (603 KB)
Verweise
[1]. Kahaly GJ, et al. A Novel Anti-CD40 Monoclonal Antibody, Iscalimab, for Control of Graves Hyperthyroidism-A Proof-of-Concept Trial. J Clin Endocrinol Metab. 2020 Mar 1;105(3):dgz013. [Content Brief]
[2]. Flandre TD, et al. Immunosuppression Profile of CFZ533 (Iscalimab), a Non-Depleting Anti-CD40 Antibody, and the Presence of Opportunistic Infections in a Rhesus Monkey Toxicology Study. Toxicol Pathol. 2022 Jul;50(5):712-724. [Content Brief]
[3]. Ristov J, et al. Characterization of the in vitro and in vivo properties of CFZ533, a blocking and non-depleting anti-CD40 monoclonal antibody. Am J Transplant. 2018 Dec;18(12):2895-2904. [Content Brief]
[4]. Cordoba F, et al. A novel, blocking, Fc-silent anti-CD40 monoclonal antibody prolongs nonhuman primate renal allograft survival in the absence of B cell depletion. Am J Transplant. 2015 Nov;15(11):2825-36. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Iscalimab (WT IgG1) Related Classifications
HY-P99670 Related Classifications
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