Art. -Nr.: HY-N2493

Lawsone

2 Cited Publications

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Based on 2 publication(s) in Google Scholar

Lawsone is an orally active naphthoquinone dye that can be isolated from the leaves of Lawsonia inermis. Lawsone can induce apoptosis. Lawsone has antibacterial, antitumor and antioxidant activities. Lawsone can be used in anti-tumor drug research.

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Reinheit: 99.54%
CAS. Nr.: 83-72-7
Formel: C₁₀H₆O₃
Molecular Weight:174.16

Speicherung:

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Lawsone Chemical Structure

CAS. Nr.: 83-72-7

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	58 μM Compound: 17	Growth inhibition of human A549 cells by MTT assay Growth inhibition of human A549 cells by MTT assay	[PMID: 24374273]
HeLa	IC₅₀	>100 μM Compound: 1c	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 24913712]
HeLa	IC₅₀	71.26 μM Compound: 1	Cytotoxicity against human HeLa cells Cytotoxicity against human HeLa cells	[PMID: 33766765]
Hepatocyte	CC₅₀	>500 μM Compound: Lawsone	Cytotoxicity activity against Swiss Webster mouse primary hepatocytes assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay Cytotoxicity activity against Swiss Webster mouse primary hepatocytes assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay	[PMID: 29289882]
HFF-1	IC₅₀	>50 μM Compound: 7	Cytotoxicity against HFF-1 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay Cytotoxicity against HFF-1 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay	[PMID: 34438124]
Hs 683	IC₅₀	31 μM Compound: 17	Growth inhibition of human Hs683 cells by MTT assay Growth inhibition of human Hs683 cells by MTT assay	[PMID: 24374273]
KB	IC₅₀	105.48 μM Compound: 1	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 33766765]
LoVo	IC₅₀	>100 μM Compound: 17	Growth inhibition of human LoVo cells by MTT assay Growth inhibition of human LoVo cells by MTT assay	[PMID: 24374273]
MCF7	IC₅₀	>50 μM Compound: 1	Cytotoxicity against ER-positive human MCF7 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay Cytotoxicity against ER-positive human MCF7 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay	[PMID: 31810778]
MDA-MB-231	IC₅₀	>50 μM Compound: 1	Cytotoxicity against triple-negative human MDA-MB-231 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay Cytotoxicity against triple-negative human MDA-MB-231 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay	[PMID: 31810778]
PC-3	IC₅₀	>100 μM Compound: 17	Growth inhibition of human PC3 cells by MTT assay Growth inhibition of human PC3 cells by MTT assay	[PMID: 24374273]
SAS	IC₅₀	>100 μM Compound: 1c	Cytotoxicity against human SAS cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human SAS cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 24913712]
SK-BR-3	IC₅₀	>50 μM Compound: 1	Cytotoxicity against HER2-positive human SKBR3 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay Cytotoxicity against HER2-positive human SKBR3 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay	[PMID: 31810778]
SK-MEL-28	IC₅₀	>100 μM Compound: 17	Growth inhibition of human SK-MEL-28 cells by MTT assay Growth inhibition of human SK-MEL-28 cells by MTT assay	[PMID: 24374273]
U-373MG ATCC	IC₅₀	>100 μM Compound: 17	Growth inhibition of human U373 cells by MTT assay Growth inhibition of human U373 cells by MTT assay	[PMID: 24374273]
Vero	CC₅₀	213.74 μM Compound: Lawsone	Cytotoxicity activity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay Cytotoxicity activity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay	[PMID: 29289882]

In Vitro

Lawsone (125, 250, 500 μM, 18 h) inhibits the growth of Escherichia coli in a dose-dependent manner^[3].
Lawsone (0.5, 1, 1.5, 2 mg/mL, 48 h) inhibits cell proliferation in human DLD-1 cells by reducing NF-κB activity, resulting in inhibition of the expression levels of cyclin B1 and cdk1^[4].
Lawsone (10, 20, 40, 80, 160 mg/mL, 24 h) has an anti-proliferation effect in SKOV-3 ovarian cancer cells and induces apoptosis by inhibiting Bcl-2^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[4]

Cell Line:

DLD-1, MRC-5
Concentration:

0.5, 1, 1.5, 2 mg/mL
Incubation Time:

24-96 h
Result:

Demonstrated significant suppression at concentration 2 mg/ml on DLD-1 cells and exhibited no suppression on normal fibroblast MRC-5 cells.

Western Blot Analysis^[4]

Cell Line:

DLD-1
Concentration:

0.5, 1, 1.5, 2 mg/mL
Incubation Time:

48 h
Result:

Decreased the level of both proteins cyclin B1 and cdk1.
Exhibited a dose-dependent effect on translocation of p65 of cytosol to the nucleus.

In Vivo

Lawsone (62.5, 125, 250, 500, 750 μmol/kg/day, orally) induces hemolytic reactions associated with oxidative damage of red blood cells in rats^[2].
Lawsone (200 mg/mL, orally, for 8 weeks) has a significant inhibitory effect on colon cancer in rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female rat model (10-11- week-old)^[2]
Dosage:

62.5, 125, 250, 500, 750 μmol/kg/day
Administration:

p.o.
Result:

Increased the levels of ALT, AST, GLDH, LDH, HBDH, urea and creatinine at a dose of 500 or 750 μmol/kg/day.

Animal Model:

Rat model of colon cancer^[4]
Dosage:

200 mg/mL
Administration:

p.o.
Result:

Reduced the tumor diameters and decreased multiplicity of these adenomas.
Decreased the PCNA staining index

Chemical Information

CAS. Nr. 83-72-7
Appearance Solid
Molecular Weight 174.16
Formel C₁₀H₆O₃
Color Light yellow to yellow
SMILES

O=C1C(O)=CC(C2=C1C=CC=C2)=O
Structure Classification
- Quinones
- Naphthalene Quinones
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Publications (2)

All Bio/Physico-chemical AssayCell Proliferation/Viability Assay

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Journal Impact Factor

Int J Biol Macromol

Identification of Vitamin K3 and its analogues as covalent inhibitors of SARS-CoV-2 3CLpro. [Abstract]

2021 Jul 31:183:182-192. PMID: 33901557
EMBO Mol Med

Modulating CCTG repeat expansion toxicity in DM2 Drosophila model through TDP1 inhibition. [Abstract]

2025 Mar 25. PMID: 40133672

Lawsone purchased from MedChemExpress. Usage Cited in: EMBO Mol Med. 2025 Mar 25. [Abstract]

Dose-response analysis of Lawsone and SPI-112. The activity of TDP1 was treated with purified TDP1 enzyme 3.125, 6.25, 12.5, 25, 50, 100, 200 μM. Data are mean ± SD from three biological replicates. IC50 values were calculated using log(inhibitor) compared with normalized response (variable slope), using the program Prism, and are given in graphs.

Lawsone purchased from MedChemExpress. Usage Cited in: EMBO Mol Med. 2025 Mar 25. [Abstract]

Quantification of γ-H2AvD staining intensity in nuclear of body-wall muscles at third instar larvae of Wild-type. Quantification at least five animals of each sample/experiment, five experiments. Data are mean ± SD. Two-tailed, unpaired t test. ***P = 0.0007 CPT versus CPT + Lawsone, ***P = 0.0003 CPT versus CPT + SPI-112.

Lawsone purchased from MedChemExpress. Usage Cited in: EMBO Mol Med. 2025 Mar 25. [Abstract]

Dose-survival analysis of Lawsone and SPI-112. The HEK293 cells were treated with purified TDP1 enzyme 0, 0.001, 0.01, 0.1, 1, 10, 100, 1000 μM for 72 h. Data are mean ± SD from three biological replicates. IC₅₀ values were calculated using log(inhibitor) compared with normalized survival rate (variable slope), using the program Prism, and are given in graphs.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (574.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.7420 mL

28.7101 mL

57.4201 mL

5 mM

1.1484 mL

5.7420 mL

11.4840 mL

10 mM

0.5742 mL

2.8710 mL

5.7420 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (14.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (14.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Tripathi RD, et al. A fungitoxic principle from the leaves of lawsonia inermis lam. Experientia. 1978 Jan 15;34(1):51-2. [Content Brief]

[2]. Munday R, et al. Haemolytic activity and nephrotoxicity of 2-hydroxy-1,4-naphthoquinone in rats. J Appl Toxicol. 1991 Apr;11(2):85-90. [Content Brief]

[3]. Sauriasari R, et al. Cytotoxicity of lawsone and cytoprotective activity of antioxidants in catalase mutant Escherichia coli. Toxicology. 2007 Jun 3;235(1-2):103-11. [Content Brief]

[4]. Wang SB, et al. Lawsone suppresses azoxymethane mediated colon cancer in rats and reduces proliferation of DLD-1 cells via NF-κB pathway. Biomed Pharmacother. 2017 May;89:152-161. [Content Brief]

[5]. Li P, et al. Lawsone inhibits cell growth and improves the efficacy of cisplatin in SKOV-3 ovarian cancer cell lines. African Journal of Traditional, Complementary and Alternative Medicines, 2017, 14(5): 8-17.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.7420 mL	28.7101 mL	57.4201 mL	143.5503 mL
	5 mM	1.1484 mL	5.7420 mL	11.4840 mL	28.7101 mL
	10 mM	0.5742 mL	2.8710 mL	5.7420 mL	14.3550 mL
	15 mM	0.3828 mL	1.9140 mL	3.8280 mL	9.5700 mL
	20 mM	0.2871 mL	1.4355 mL	2.8710 mL	7.1775 mL
	25 mM	0.2297 mL	1.1484 mL	2.2968 mL	5.7420 mL
	30 mM	0.1914 mL	0.9570 mL	1.9140 mL	4.7850 mL
	40 mM	0.1436 mL	0.7178 mL	1.4355 mL	3.5888 mL
	50 mM	0.1148 mL	0.5742 mL	1.1484 mL	2.8710 mL
	60 mM	0.0957 mL	0.4785 mL	0.9570 mL	2.3925 mL
	80 mM	0.0718 mL	0.3589 mL	0.7178 mL	1.7944 mL
	100 mM	0.0574 mL	0.2871 mL	0.5742 mL	1.4355 mL

Keywords

Lawsone

2 Cited Publications

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Publications Citing Use of MedChemExpress (MCE) Lawsone

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Lawsone Related Classifications

HY-N2493 Related Classifications