Art. -Nr.: HY-N6686

Virginiamycin M1

(Synonyms: Pristinamycin IIA; Ostreogrycin A)

2 Cited Publications

Kundenbewertung

Based on 2 publication(s) in Google Scholar

Virginiamycin M1 (Pristinamycin IIA; Ostreogrycin A), produced by?Streptomyces virginiae, is an polyunsaturated macrocyclic lactone antibiotic and acts as a component of Virginiamycin (HY-112665). Virginiamycin M1 alone is against Staphylococcus aureus with a MIC of 0.25 μg/mL.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.13%
CAS. Nr.: 21411-53-0
Formel: C₂₈H₃₅N₃O₇
Molecular Weight:525.59

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Virginiamycin M1 Chemical Structure

CAS. Nr.: 21411-53-0

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Virginiamycin M1 (Pristinamycin IIA; Ostreogrycin A), produced by?Streptomyces virginiae, is an polyunsaturated macrocyclic lactone antibiotic and acts as a component of Virginiamycin (HY-112665)^[1]. Virginiamycin M1 alone is against Staphylococcus aureus with a MIC of 0.25 μg/mL.

IC₅₀ & Target

Macrolide

Alle Antibiotic Isoform-spezifische Produkte anzeigen :

In Vitro

Virginiamycin is an animal-specific antibiotic containing a lactone ring. Virginiamycin is not a single molecule but contains 70-80% of the M1 component (M1 factor) and 20-30% of the S1 component (S1 factor). Virginiamycin M1 is a macrolide and Virginiamycin S1 is a cyclic polypeptide. The minimum inhibitory concentration (MIC) of the individual Virginiamycin M1 component against Staphylococcus aureus is 0.25 μg/mL, and the Virginiamycin S1 component is 4 μg/mL, whereas the combination of the two is 0.125 μg/mL, which improves the bactericidal effect 1~30 times^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 21411-53-0
Appearance Solid
Molecular Weight 525.59
Formel C₂₈H₃₅N₃O₇
Color White to yellow
SMILES

O=C1N2C(C(O[C@@H]([C@@H](/C=C/C(NC/C=C\C(C)=C\[C@H](CC(CC3=NC1=CO3)=O)O)=O)C)C(C)C)=O)=CCC2
Synonyms

Pristinamycin IIA; Ostreogrycin A
Structure Classification
- Antibiotics
- Macrolide Antibiotics
Initial Source
- Microorganisms
Streptomyces pristinaespiralis
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (2)

All

Sort by:

Journal Impact Factor

Microb Biotechnol

Attenuator LRR - a regulatory tool for modulating gene expression in Gram-positive bacteria. [Abstract]

2021 Nov;14(6):2538-2551. PMID: 33720523
SSRN

2026 Mar 3.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (190.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9026 mL

9.5131 mL

19.0262 mL

5 mM

0.3805 mL

1.9026 mL

3.8052 mL

10 mM

0.1903 mL

0.9513 mL

1.9026 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (9.51 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (9.51 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (9.51 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. N Suzuki, et al. Purification and characterization of virginiamycin M1 reductase from Streptomyces virginiae. Antimicrob Agents Chemother. 1998 Nov;42(11):2985-8. [Content Brief]

[2]. Xiaoxin Xu, et al. Gold nanoparticle-based lateral flow strips for rapid and sensitive detection of Virginiamycin M1. Pages 764-777 | Received 18 Mar 2020, Accepted 27 Apr 2020,

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9026 mL	9.5131 mL	19.0262 mL	47.5656 mL
	5 mM	0.3805 mL	1.9026 mL	3.8052 mL	9.5131 mL
	10 mM	0.1903 mL	0.9513 mL	1.9026 mL	4.7566 mL
	15 mM	0.1268 mL	0.6342 mL	1.2684 mL	3.1710 mL
	20 mM	0.0951 mL	0.4757 mL	0.9513 mL	2.3783 mL
	25 mM	0.0761 mL	0.3805 mL	0.7610 mL	1.9026 mL
	30 mM	0.0634 mL	0.3171 mL	0.6342 mL	1.5855 mL
	40 mM	0.0476 mL	0.2378 mL	0.4757 mL	1.1891 mL
	50 mM	0.0381 mL	0.1903 mL	0.3805 mL	0.9513 mL
	60 mM	0.0317 mL	0.1586 mL	0.3171 mL	0.7928 mL
	80 mM	0.0238 mL	0.1189 mL	0.2378 mL	0.5946 mL
	100 mM	0.0190 mL	0.0951 mL	0.1903 mL	0.4757 mL

Keywords

Virginiamycin M1

2 Cited Publications

100 mg Angebot einholen

Subscribe to the

MedChemExpress

Newsletter

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

Virginiamycin M1

Anti-Infection Compound Library

Natural Product Library

Covalent Screening Library

Macrocyclic Compound Library

Antibacterial Compound Library

Antibiotics Library

Microbial Metabolite Library

Human Metabolite Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Metabolite Library

Natural Product Diversity Scaffold Library

Fungal-Sourced Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Virginiamycin M1

Alle Antibiotic Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Virginiamycin M1

Anti-Infection Compound Library

Natural Product Library

Covalent Screening Library

Macrocyclic Compound Library

Antibacterial Compound Library

Antibiotics Library

Microbial Metabolite Library

Human Metabolite Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Metabolite Library

Natural Product Diversity Scaffold Library

Fungal-Sourced Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Virginiamycin M1

Alle Antibiotic Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Virginiamycin M1 Related Classifications

HY-N6686 Related Classifications