Cat. No.: HY-B0035

Sulfamethazine

(Synonyms: Sulfadimidine; Sulfadimerazine)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Sulfamethazine (Sulfadimidine) is a brain-penetrant sulfonamide antibiotic. Sulfamethazine inhibits bacterial growth by blocking the synthesis of folic acid. Sulfamethazine can be used for research on bacterial infections.

For research use only. We do not sell to patients.

Purity: 99.47%
CAS No.: 57-68-1
Formula: C₁₂H₁₄N₄O₂S
Molecular Weight:278.33

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Sulfamethazine Chemical Structure

CAS No.: 57-68-1

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Biological Activity
- Description
- IC₅₀ & Target
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Bacterial^[1]

All Antibiotic Isoforms :

In Vivo

Sulfamethazine (0.3 g/kg; i.m.; single dose) demonstrates restricted passage across the blood-brain barrier in adult pregnant and non-pregnant Wistar rats, with brain levels consistently reaching 30-37% of corresponding blood levels^[1].
Sulfamethazine (80 mg/kg; i.v.; single dose) treatment significantly reduced α, β and AUC_0-∞, and significantly increased t_1/2α, V_d and CIB, with an absolute bioavailability of 1.01^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Wistar rats (adult female, pregnant and non-pregnant)^[2]
Dosage:

0.3 g/kg
Administration:

i.m.; single dose
Result:

Measured blood sulfamethazine level of 17.27 mg/100 mL and brain level of 5.71 mg/100 mL (33% of blood level) at 60 minutes in gestational day 10 pregnant rats receiving carbadox.
Reached peak blood sulfamethazine level of 22.23 mg/100 mL and brain level of 8.30 mg/100 mL (37% of blood level) at 120 minutes in gestational day 10 pregnant rats receiving carbadox.
Measured blood sulfamethazine level of 15.55 mg/100 mL and brain level of 5.11 mg/100 mL (32% of blood level) at 240 minutes in gestational day 10 pregnant rats receiving carbadox.
Measured blood sulfamethazine level of 23.44 mg/100 mL and brain level of 7.35 mg/100 mL (31% of blood level) at 60 minutes in gestational day 20 pregnant rats receiving carbadox.
Reached peak blood sulfamethazine level of 31.51 mg/100 mL and brain level of 11.11 mg/100 mL (35% of blood level) at 120 minutes in gestational day 20 pregnant rats receiving carbadox.
Measured blood sulfamethazine level of 25.20 mg/100 mL and brain level of 8.30 mg/100 mL (32% of blood level) at 240 minutes in gestational day 20 pregnant rats receiving carbadox.
Measured blood sulfamethazine level of 17.24 mg/100 mL and brain level of 5.15 mg/100 mL (30% of blood level) at 60 minutes in gestational day 10 pregnant rats not receiving carbadox.
Reached peak blood sulfamethazine level of 22.75 mg/100 mL and brain level of 8.25 mg/100 mL (36% of blood level) at 120 minutes in gestational day 10 pregnant rats not receiving carbadox.
Measured blood sulfamethazine level of 15.80 mg/100 mL and brain level of 5.15 mg/100 mL (32% of blood level) at 240 minutes in gestational day 10 pregnant rats not receiving carbadox.
Measured blood sulfamethazine level of 23.78 mg/100 mL and brain level of 8.05 mg/100 mL (33% of blood level) at 60 minutes in gestational day 20 pregnant rats not receiving carbadox.
Reached peak blood sulfamethazine level of 30.72 mg/100 mL and brain level of 10.83 mg/100 mL (35% of blood level) at 120 minutes in gestational day 20 pregnant rats not receiving carbadox.
Measured blood sulfamethazine level of 25.68 mg/100 mL and brain level of 8.77 mg/100 mL (34% of blood level) at 240 minutes in gestational day 20 pregnant rats not receiving carbadox.
Observed slightly higher blood sulfamethazine levels in pregnant rats than non-pregnant counterparts, and higher levels in 20-day pregnant rats than 10-day pregnant rats.
Detected no significant difference in sulfamethazine levels in blood and brain between rats receiving carbadox and those that did not.

Chemical Information

CAS No. 57-68-1
Appearance Solid
Molecular Weight 278.33
Formula C₁₂H₁₄N₄O₂S
Color White to off-white
SMILES

O=S(C1=CC=C(N)C=C1)(NC2=NC(C)=CC(C)=N2)=O
Synonyms

Sulfadimidine; Sulfadimerazine
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (3)

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Journal Impact Factor

Chemosphere

Mass-balance-model-based evaluation of sewage treatment plant contribution to residual pharmaceuticals in environmental waters. [Abstract]

2019 Jun:225:378-387. PMID: 30884299
ACS Environ Au

Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]

2025 Aug 5;5(6):573-582. PMID: 41277996
Anal Chem

Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]

2025 Jun 3;97(21):11099-11109. PMID: 40401576

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (359.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5929 mL

17.9643 mL

35.9286 mL

5 mM

0.7186 mL

3.5929 mL

7.1857 mL

10 mM

0.3593 mL

1.7964 mL

3.5929 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (290 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. VAN Poucke LSG, et al. Pharmacokinetics and Tissue Residues of Sulfathiazole and Sulfamethazine in Pigs. J Food Prot. 1994 Sep;57(9):796-801. [Content Brief]

[2]. Sheng Y J, et al. Production of chicken yolk IgY to sulfamethazine: comparison with rabbit antiserum IgG. Food and Agricultural Immunology. 2015, 26(3):305-316.

[3]. Siddique A B, et al. Effect of Carbadox on the Passage of Sulfamethazine through the Blood-Brain Barrier in Rat[J]. Acta Veterinaria Brno, 1979, 48(1-4): 79-84.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5929 mL	17.9643 mL	35.9286 mL	89.8214 mL
	5 mM	0.7186 mL	3.5929 mL	7.1857 mL	17.9643 mL
	10 mM	0.3593 mL	1.7964 mL	3.5929 mL	8.9821 mL
	15 mM	0.2395 mL	1.1976 mL	2.3952 mL	5.9881 mL
	20 mM	0.1796 mL	0.8982 mL	1.7964 mL	4.4911 mL
	25 mM	0.1437 mL	0.7186 mL	1.4371 mL	3.5929 mL
	30 mM	0.1198 mL	0.5988 mL	1.1976 mL	2.9940 mL
	40 mM	0.0898 mL	0.4491 mL	0.8982 mL	2.2455 mL
	50 mM	0.0719 mL	0.3593 mL	0.7186 mL	1.7964 mL
	60 mM	0.0599 mL	0.2994 mL	0.5988 mL	1.4970 mL
	80 mM	0.0449 mL	0.2246 mL	0.4491 mL	1.1228 mL
	100 mM	0.0359 mL	0.1796 mL	0.3593 mL	0.8982 mL

Keywords

Sulfamethazine

3 Cited Publications

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Sulfamethazine

Anti-Infection Compound Library

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Publications Citing Use of MedChemExpress (MCE) Sulfamethazine

All Antibiotic Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Sulfamethazine

Anti-Infection Compound Library

FDA-Approved Drug Library

CNS-Penetrant Compound Library

Peptidomimetic Library

Drug Repurposing Compound Library

Antibacterial Compound Library

NMPA-Approved Drug Library

FDA Approved & Pharmacopeial Drug Library

Antibiotics Library

Human Metabolite Library

Off-patent Drug Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Animal Drug Library

Publications Citing Use of MedChemExpress (MCE) Sulfamethazine

All Antibiotic Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Sulfamethazine Related Classifications

HY-B0035 Related Classifications