Dipropofol
Based on 1 Customer Validation
Dipropofol is a dimeric derivative of Propofol (HY-B0649) with antioxidant and antibacterial activities. Dipropofol terminates free radical chain reactions, scavenges DPPH free radicals and inhibits lipid peroxidation. Dipropofol protects cells from death induced by glutamate, Aβ(25-35) and buthionine sulfoximine, and increases the survival rate of mice exposed to oxygen. Dipropofol selectively activates tonic GABAA currents, hyperpolarizes resting membrane potential, reduces action potential firing, and alleviates the severity of seizures induced by Kainic acid. Dipropofol can be used in the research of Gram-positive bacterial infections, Alzheimer's disease, cerebral ischemia-related neuronal injury, oxygen toxicity and temporal lobe epilepsy.
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- Pureté: 99.66%
- CAS No.: 2416-95-7
- Formule: C24H34O2
- Masse moléculaire:354.53
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Stockage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Dipropofol (62.5-250 μM; 30 min) potently inhibits Fe2+- and ascorbate-induced lecithin lipid peroxidation, with an inhibition rate of 98.2% at 62.5 μM and 98.8% at 250 μM[1].
Dipropofol scavenges DPPH free radicals with an IC50 value of 80 μM[1].
Dipropofol (62.5-80 μM) exhibits in vitro antioxidant activity, with an inhibition rate of 98% against lipid peroxidation at a concentration of 62.5 μM and a DPPH IC50 of 80 μM[2].
Dipropofol (62.5-1000 nM) completely inhibits glutamate-induced cytotoxicity in N18-RE-105 cells at concentrations of 62.5 nM and above[2].
Dipropofol (1-10 μM) completely reverses the cytotoxicity induced by Aβ(25-35) (HY-P0128) in PC12 cells and primary fetal rat hippocampal cells[2].
Dipropofol exhibits potent in vitro antibacterial activity against Gram-positive bacteria (including MRSA and VRE), with MIC values of 2-4 μg/mL, while it shows no activity against Gram-negative bacteria (MIC >128 μg/mL)[2].
Dipropofol (10 μM) hyperpolarizes the resting membrane potential of layer II stellate neurons in the mouse medial entorhinal cortex (mEC) in acute brain slices, reduces action potential firing, and thereby inhibits their excitability; when the membrane potential is clamped at a constant level, this drug has no effect on the intrinsic excitability of neurons[3].
Dipropofol (0.1-100 μM) activates endogenous tonic GABAA currents in layer II stellate neurons of the mouse medial entorhinal cortex (mEC) in acute brain slices, with an EC50 of 9.3 μM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (male, 18-20 g, specific pathogen-free grade, Kainic acid-induced acute seizures model)[3]
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Dosage:50 mg/kg
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Administration:i.p.; 2 total doses: first dose 35 min pre-Kainic acid, second dose 5 min pre-Kainic acid
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Result:Slowed seizure progression significantly, with lower mean maximum seizure classes recorded at 15, 30, 45, 60, and 90 min after Kainic acid injection compared to vehicle controls.
Increased latency to first generalized (class 4) seizure to 36 min.
Reduced incidence of generalized class 4 seizures to 45.5% (5/11) compared to 92.3% (12/13) in vehicle-treated mice.
Decreased the number of mice that progressed to class 5 seizures compared to vehicle controls.
Chemical Information
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CAS No. 2416-95-7
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Appearance Solid
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Masse moléculaire 354.53
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Formule C24H34O2
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Color White to light yellow
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SMILES
OC1=C(C(C)C)C=C(C2=CC(C(C)C)=C(O)C(C(C)C)=C2)C=C1C(C)C
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvant et solubilité
DMSO : 100 mg/mL (282.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Ogata M, et al. Antioxidant activity of propofol and related monomeric and dimeric compounds. Chemical & pharmaceutical bulletin. 2005 Mar;53(3):344-6. [Content Brief]
[2]. Ogata M, et al. [Antioxidant and antibacterial activities of dimeric phenol compounds]. Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan. 2008 Aug;128(8):1149-58. [Content Brief]
[3]. Zhang J, et al. Anticonvulsant effect of dipropofol by enhancing native GABA currents in cortical neurons in mice. J Neurophysiol. 2018 Sep 1;120(3):1404-1414. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8206 mL | 14.1032 mL | 28.2064 mL | 70.5159 mL |
| 5 mM | 0.5641 mL | 2.8206 mL | 5.6413 mL | 14.1032 mL | |
| 10 mM | 0.2821 mL | 1.4103 mL | 2.8206 mL | 7.0516 mL | |
| 15 mM | 0.1880 mL | 0.9402 mL | 1.8804 mL | 4.7011 mL | |
| 20 mM | 0.1410 mL | 0.7052 mL | 1.4103 mL | 3.5258 mL | |
| 25 mM | 0.1128 mL | 0.5641 mL | 1.1283 mL | 2.8206 mL | |
| 30 mM | 0.0940 mL | 0.4701 mL | 0.9402 mL | 2.3505 mL | |
| 40 mM | 0.0705 mL | 0.3526 mL | 0.7052 mL | 1.7629 mL | |
| 50 mM | 0.0564 mL | 0.2821 mL | 0.5641 mL | 1.4103 mL | |
| 60 mM | 0.0470 mL | 0.2351 mL | 0.4701 mL | 1.1753 mL | |
| 80 mM | 0.0353 mL | 0.1763 mL | 0.3526 mL | 0.8814 mL | |
| 100 mM | 0.0282 mL | 0.1410 mL | 0.2821 mL | 0.7052 mL |
Dipropofol Related Classifications
HY-W101673 Related Classifications
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