pPB peptide
Based on 1 Customer Validation
pPB peptide is a cyclic peptide targeting platelet-derived growth factor receptor β (PDGFRβ). Derived from natural platelet-derived growth factor (PDGF), pPB peptide contains arginine and isoleucine as key receptor-binding residues. pPB peptide specifically binds to PDGFRβ, which is highly expressed on the surface of activated hepatic stellate cells (HSC). pPB peptide can be used in studies related to liver fibrosis, liver cirrhosis, and tumor-specific drug delivery.
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- Pureté : 99.03%
- CAS No.: 663933-94-6
- Formule: C35H60N12O13S2
- Masse moléculaire:921.05
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Stockage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
IC50 & Target
[1]|
PDGFRβ |
In Vitro
The pPB peptide is successfully conjugated to pegylated hollow polydopamine nanoparticles, with a molar grafting ratio of 1.50%[1].
The pPB peptide can be conjugated to form DSPE-PEG-pPB[2].
Modification with pPB peptide (6 weeks) does not significantly alter the particle size distribution, charge or morphology of SNALPs, and SNALPs modified with 2% pPB remain stable for at least 6 weeks[2].
The pPB peptide mediates enhanced uptake of SNALPs by hepatic stellate cells (HSCs) through specific binding to the PDGFR-β receptor on HSCs[2].
Modification with the pPB peptide enhances the gene silencing effects on HSP47 and HMGB1 in hepatic stellate cells (HSCs) by improving the uptake efficiency of SNALP[2].
The pPB peptide successfully conjugates with DMPE-PEG-Mal to form the DPP construct[3].
PPB peptide (1 μg/mL; 2 h) mediates PDGFβR-specific, species-nonspecific binding of IFNγ conjugates to mouse NIH3T3 fibroblasts, human LX2 hepatic stellate cells, and primary rat hepatic stellate cells[4].
The PPB peptide (2 h) significantly reduces TGFβ1-induced collagen expression in mouse NIH3T3 fibroblasts and human LX2 hepatic stellate cells when combined with IFNγ, overcoming the limitation of species-specific IFNγ receptors in human cells[4].
Conjugation of the PPB peptide with IFNγ mediates the inhibition of PDGF-induced proliferation of mouse NIH3T3 fibroblasts[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 663933-94-6
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Appearance Solid
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Masse moléculaire 921.05
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Formule C35H60N12O13S2
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Color White to off-white
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Sequence
Cys-Ser-Arg-Asn-Leu-Ile-Asp-Cys (disulfide bridge:Cys1-Cys8)
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Sequence Shortening
CSRNLIDC (disulfide bridge:Cys1-Cys8)
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
In Vitro:
DMSO : ≥ 50 mg/mL (54.29 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.71 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (2.71 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (289 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.0857 mL | 5.4286 mL | 10.8572 mL | 27.1429 mL |
| 5 mM | 0.2171 mL | 1.0857 mL | 2.1714 mL | 5.4286 mL | |
| 10 mM | 0.1086 mL | 0.5429 mL | 1.0857 mL | 2.7143 mL | |
| 15 mM | 0.0724 mL | 0.3619 mL | 0.7238 mL | 1.8095 mL | |
| 20 mM | 0.0543 mL | 0.2714 mL | 0.5429 mL | 1.3571 mL | |
| 25 mM | 0.0434 mL | 0.2171 mL | 0.4343 mL | 1.0857 mL | |
| 30 mM | 0.0362 mL | 0.1810 mL | 0.3619 mL | 0.9048 mL | |
| 40 mM | 0.0271 mL | 0.1357 mL | 0.2714 mL | 0.6786 mL | |
| 50 mM | 0.0217 mL | 0.1086 mL | 0.2171 mL | 0.5429 mL |
pPB peptide Related Classifications
HY-P11549 Related Classifications
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