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Cat. No.: HY-114164D

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Rat Thrombin

8 Cited Publications

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Based on 8 publication(s) in Google Scholar

Rat Thrombin is a Na⁺-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.

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CAS No.: 9002-04-4

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Please store the product under the recommended conditions in the Certificate of Analysis.

Rat Thrombin Chemical Structure

CAS No.: 9002-04-4

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
Chemical Information
Publications (8)
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Rat Thrombin is a Na⁺-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins^[1].

In Vitro

p62 is cleaved from the agarose beads as well as the GST tag by addition of Rat Thrombin (1 Unit/100 μg protein) at room temperature for 20 h^[3].
Rat Thrombin solution: Dissolve in PBS prechilled to 4°C. Swirl gently. Store solution in small aliquots at -80°C to preserve activity.
Cleavage buffer: PBS (140 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄) pH 7.3.
Elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 9002-04-4
Appearance Solid
Color White to off-white
SMILES

[Rat Thrombin]
Livraison

Shipping with dry ice.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (8)

All Flow CytometryWBCell Imaging/StainingELISACell Migration/Invasion Assay

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Cancer Commun (Lond)

Reciprocal tumor-platelet interaction through the EPHB1-EFNB1 axis in the liver metastatic niche promotes metastatic tumor outgrowth in pancreatic ductal adenocarcinoma. [Abstract]

2025 Feb;45(2):143-166. PMID: 39648610

Rat Thrombin purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Feb;45(2):143-166. [Abstract]

Platelets were treated with Fc fragment, Thrombin (1 µg/mL; 2 h), EphB1‐Fc, Thrombin + EphB1‐Fc, mono EphB1, mono EphB1 + EphB1‐Fc, or EFNB1 Ab + EphB1‐Fc. Platelets without treatment served as controls. CD62P expression in activated platelets was tested by flow cytometry analysis. Representative flow cytometry analysis of CD62P expression is presented on the left. The right graph shows the percentage of CD62P+ platelets. Data were presented as the means ± SD, with n = 3 per group.
Allergy

Thrombin cleaves IL-33 and modulates IL-33-activated allergic lung inflammation. [Abstract]

2022 Jul;77(7):2104-2120. PMID: 34995358

Rat Thrombin purchased from MedChemExpress. Usage Cited in: Allergy. 2022 Jul;77(7):2104-2120. [Abstract]

Western blots for the HA tag on RRL-expressed IL-33 showing the Thrombin cleavage of human pro-IL-33 into the two main forms, ~18 and ~25 kDa, and the inhibition of these cleavage events by the Thrombin inhibitor Argatroban (50 μg/ml). Cleavage occurred in a time-dependent manner (15 min, 30 min, 1 h, 2 h and 4 h; 0.075 U Thrombin; 15 μL buffer) (A) and a dose-dependent manner (0.01875, 0.0375, 0.075, 0.15 and 0.3 U; RT for 1 h; 15 μL buffer) (B).

Rat Thrombin purchased from MedChemExpress. Usage Cited in: Allergy. 2022 Jul;77(7):2104-2120. [Abstract]

L-33-dependent IL-5, IL-13 and IL-4 production by ST2+ CD4 cells incubated with or without Thrombin (50 pg RRL; 0.075 U Thrombin; RT for 1 h) and with or without a neutralizing hIL-33 mAb. The bars and error bars represent the mean ± SEM; *p < .05; **p < .01; ***p < .001.
Stem Cell Res Ther

Effects of SW033291 on the myogenesis of muscle-derived stem cells and muscle regeneration. [Abstract]

2020 Feb 21;11(1):76. PMID: 32085799
Front Pharmacol

Hsp47 Inhibitor Col003 Attenuates Collagen-Induced Platelet Activation and Cerebral Ischemic-Reperfusion Injury in Rats. [Abstract]

2022 Jan 10;12:792263. PMID: 35082674
Antiviral Res

Omicsynin B4 potently blocks coronavirus infection by inhibiting host proteases cathepsin L and TMPRSS2. [Abstract]

2023 Jun:214:105606. PMID: 37076089
Mol Carcinog

Baicalein promotes KDM4E to induce BICD1 and inhibit triple-negative breast cancer progression by blocking PAR1 signaling. [Abstract]

2024 Jul;63(7):1288-1302. PMID: 38607237

Rat Thrombin purchased from MedChemExpress. Usage Cited in: Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]

Effect of knockdown of KDM4E and combined overexpression of BICD1 on the proliferative activity of Thrombin (1 pM)-activated TNBC cells detected by EdU staining (scale bar: 50 μm).

Rat Thrombin purchased from MedChemExpress. Usage Cited in: Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]

Effect of knockdown of KDM4E and combined overexpression of BICD1 on the invasive activity of Thrombin (1 pM)-activated TNBC cells detected by Transwell assay (scale bar: 50 μm). *p < 0.05 versus the sh-scramble group, #p < 0.05 versus the sh-KDM4E + oe-NC group, &p < 0.05 versus the blank group.
University of Utah

2026.
bioRxiv

Protease-Activated Receptor 1 as an Endogenous Model of Peptidergic Gαq-Gα12-Biased G Protein Signaling. [Abstract]

2026 Mar 5:2026.03.03.709348. PMID: 41847045

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (263 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Golderman V, et al. Thrombin and the Protease-Activated Receptor-1 in Organophosphate-Induced Status Epilepticus. J Mol Neurosci. 2018 Dec 4. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Rat Thrombin

8 Cited Publications

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Activité biologique
- Description
- In Vitro
Chemical Information
Publications (8)
Pureté et documentation
Références

1/16
1/16

Rat Thrombin purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Feb;45(2):143-166. [Abstract]

Customer Validation
Platelets were treated with Fc fragment, Thrombin (1 µg/mL; 2 h), EphB1‐Fc, Thrombin + EphB1‐Fc, mono EphB1, mono EphB1 + EphB1‐Fc, or EFNB1 Ab + EphB1‐Fc. Platelets without treatment served as controls. CD62P expression in activated platelets was tested by flow cytometry analysis. Representative flow cytometry analysis of CD62P expression is presented on the left. The right graph shows the percentage of CD62P+ platelets. Data were presented as the means ± SD, with n = 3 per group.
1/16

Rat Thrombin purchased from MedChemExpress. Usage Cited in: Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]

Customer Validation
Effect of knockdown of KDM4E and combined overexpression of BICD1 on the proliferative activity of Thrombin (1 pM)-activated TNBC cells detected by EdU staining (scale bar: 50 μm).
1/16

Rat Thrombin purchased from MedChemExpress. Usage Cited in: Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]

Customer Validation
Effect of knockdown of KDM4E and combined overexpression of BICD1 on the invasive activity of Thrombin (1 pM)-activated TNBC cells detected by Transwell assay (scale bar: 50 μm). *p < 0.05 versus the sh-scramble group, #p < 0.05 versus the sh-KDM4E + oe-NC group, &p < 0.05 versus the blank group.
1/16

Rat Thrombin purchased from MedChemExpress. Usage Cited in: Allergy. 2022 Jul;77(7):2104-2120. [Abstract]

Customer Validation
Western blots for the HA tag on RRL-expressed IL-33 showing the Thrombin cleavage of human pro-IL-33 into the two main forms, ~18 and ~25 kDa, and the inhibition of these cleavage events by the Thrombin inhibitor Argatroban (50 μg/ml). Cleavage occurred in a time-dependent manner (15 min, 30 min, 1 h, 2 h and 4 h; 0.075 U Thrombin; 15 μL buffer) (A) and a dose-dependent manner (0.01875, 0.0375, 0.075, 0.15 and 0.3 U; RT for 1 h; 15 μL buffer) (B).
1/16

Rat Thrombin purchased from MedChemExpress. Usage Cited in: Allergy. 2022 Jul;77(7):2104-2120. [Abstract]

Customer Validation
L-33-dependent IL-5, IL-13 and IL-4 production by ST2+ CD4 cells incubated with or without Thrombin (50 pg RRL; 0.075 U Thrombin; RT for 1 h) and with or without a neutralizing hIL-33 mAb. The bars and error bars represent the mean ± SEM; *p < .05; **p < .01; ***p < .001.

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Rat Thrombin

Publications Citing Use of MedChemExpress (MCE) Rat Thrombin

Activité biologique

Chemical Information

Publications (8)

Pureté et documentation

Références

Calculators

Help & FAQs

Rat Thrombin Related Classifications

HY-114164D Related Classifications