Camylofine
Based on 1 Customer Validation
Camylofine is an antispasmodic agent with myogenic and neurogenic effects. Camylofine exhibits mild anticholinergic activity and blocks the M3 receptors on colonic smooth muscle. Camylofine dihydrochloride inhibits phosphodiesterase, elevates intracellular cAMP levels and reduces intracellular Ca2+ levels, thereby promoting smooth muscle relaxation. Camylofine can be used in studies related to antispasmodia, gastrointestinal smooth muscle and ulcerative colitis.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 98.21%
- CAS. Nr.: 54-30-8
- Formel: C19H32N2O2
- Molecular Weight:320.47
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Speicherung:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
M3 receptors |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| HEK293 | IC50 |
9.12 μM
Compound: Camylofine
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Inhibition of human OCT1 expressed in HEK293 cells assessed as decrease in uptake of ASP+ after 2 mins by fluorescence assay
Inhibition of human OCT1 expressed in HEK293 cells assessed as decrease in uptake of ASP+ after 2 mins by fluorescence assay
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[PMID: 28230985] |
In Vitro
The cumulative release rate of Camylofine dihydrochloride from Camylofine dihydrochloride-loaded Eudragit® S-100-coated chitosan microspheres reaches 72.5% in the presence of 3% w/v rat caecal content within 24 h, while it is 59.2% in the absence of rat caecal content[1].
After incubation for 24 h in a release system containing 3% w/v cecal contents from enzyme-induced rats treated for 5 days, the cumulative release rate of Camylofine dihydrochloride from Camylofine dihydrochloride-loaded Eudragit® S-100-coated chitosan microspheres reaches 95.5%[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Aqueous solution of Camylofine dihydrochloride (0.71 mg/kg; oral administration) reduces the Ca2+ concentration in colonic smooth muscle tissue to 1.989 mg/g colon in TNBS-induced colitis rats, whereas the concentration is 3.807 mg/g colon in the colitis control group[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Albino rats (either sex, 10-12 weeks old, 100-150 g, TNBS-induced ulcerative colitis)[1]
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Dosage:0.71 mg/kg
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Administration:p.o. (Camylofine dihydrochloride aqueous solution); repeated dosing; 11 days
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Result:Resulted in a colonic smooth-muscle Ca²⁺ concentration of 1.989 mg/g colon versus 3.807 mg/g colon in the colitis control group.
Chemical Information
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CAS. Nr. 54-30-8
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Appearance Liquid (Density: 0.988±0.06 g/cm3)
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Molecular Weight 320.47
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Formel C19H32N2O2
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Color Light yellow to brown
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SMILES
O=C(OCCC(C)C)C(NCCN(CC)CC)C1=CC=CC=C1
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (312.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 10 mg/mL (31.20 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.80 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (277 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.1204 mL | 15.6021 mL | 31.2042 mL | 78.0104 mL |
| 5 mM | 0.6241 mL | 3.1204 mL | 6.2408 mL | 15.6021 mL | |
| 10 mM | 0.3120 mL | 1.5602 mL | 3.1204 mL | 7.8010 mL | |
| 15 mM | 0.2080 mL | 1.0401 mL | 2.0803 mL | 5.2007 mL | |
| 20 mM | 0.1560 mL | 0.7801 mL | 1.5602 mL | 3.9005 mL | |
| 25 mM | 0.1248 mL | 0.6241 mL | 1.2482 mL | 3.1204 mL | |
| 30 mM | 0.1040 mL | 0.5201 mL | 1.0401 mL | 2.6003 mL | |
| DMSO | 40 mM | 0.0780 mL | 0.3901 mL | 0.7801 mL | 1.9503 mL |
| 50 mM | 0.0624 mL | 0.3120 mL | 0.6241 mL | 1.5602 mL | |
| 60 mM | 0.0520 mL | 0.2600 mL | 0.5201 mL | 1.3002 mL | |
| 80 mM | 0.0390 mL | 0.1950 mL | 0.3901 mL | 0.9751 mL | |
| 100 mM | 0.0312 mL | 0.1560 mL | 0.3120 mL | 0.7801 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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