Prazosin
Based on 18 publication(s) in Google Scholar
Prazosin is an α-adrenergic receptor antagonist. Prazosin can reduce inflammation, relieve anxiety, alleviate panic, prevent memory decline, and modulate the pain-relieving effects of opioids. Prazosin can be used in the study of hypertension and Alzheimer’s disease.
For research use only. We do not sell to patients.
- Purity: 99.80%
- CAS No.: 19216-56-9
- Formula: C19H21N5O4
- Molecular Weight:383.40
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Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Prazosin
More- Nature. 2025 Dec;648(8092):146-156. [Abstract]
- Cancer Discov. 2026 Feb 9. [Abstract]
- Cell Discov. 2023 Feb 7;9(1):16. [Abstract]
- Cell Rep Med. 2024 Oct 1:101771. [Abstract]
- J Exp Med. 2023 Nov 6;220(11):e20230577. [Abstract]
- J Transl Med. 2022 Oct 2;20(1):444. [Abstract]
- J Med Chem. 2021 Mar 11;64(5):2725-2738. [Abstract]
- Redox Rep. 2025 Dec;30(1):2494314. [Abstract]
- Mol Metab. 2026 May 26:102384. [Abstract]
- Drug Des Devel Ther. 2024 Apr 9:18:1103-1114. [Abstract]
- Cells. 2023 May 24;12(11):1461. [Abstract]
- Am J Pathol. 2023 Jul;193(7):913-926. [Abstract]
- iScience. 2025 Jan 17;28(2):111831. [Abstract]
- Am J Physiol Renal Physiol. 2024 Nov 1;327(5):F885-F898. [Abstract]
- BMC Pulm Med. 2021 Jun 5;21(1):189. [Abstract]
- bioRxiv. 2024 November 03.
- Elife. 2024 May 29.
- Biomed Pharmacother. 2020 Apr;124:109731. [Abstract]
Customer Validation & Images
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WB
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ELISA
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WB
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Flow Cytometry
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Cell Proliferation/Viability Assay
All Adrenergic Receptor Isoforms
More
Biological Activity
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Cell Line
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Type | Value | Description | References |
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| HEK293 | IC50 |
>100 μM
Compound: Prazosin
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TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT2-expressing HEK293 cells
TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT2-expressing HEK293 cells
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[PMID: 12110607] |
| HEK293 | IC50 |
0.88 nM
Compound: prazosin
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Displacement of [3H]Prazosin from human recombinant alpha1D adrenergic receptor expressed in HEK293 cells after 60 mins
Displacement of [3H]Prazosin from human recombinant alpha1D adrenergic receptor expressed in HEK293 cells after 60 mins
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[PMID: 23403082] |
| HEK293 | IC50 |
1.6 μM
Compound: prazosin
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Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay
Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay
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[PMID: 23241029] |
| HEK293 | IC50 |
1.84 μM
Compound: Prazosin
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TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT1-expressing HEK293 cells
TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT1-expressing HEK293 cells
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[PMID: 12110607] |
| HEK293 | IC50 |
12.6 μM
Compound: Prazosin
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TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT3-expressing HEK293 cells
TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT3-expressing HEK293 cells
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[PMID: 12110607] |
| HEK293 | IC50 |
13.6 μM
Compound: prazosin
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Inhibition of human OCT2-mediated ASP+ uptake expressed in HEK293 cells after 3 mins by fluorescence assay
Inhibition of human OCT2-mediated ASP+ uptake expressed in HEK293 cells after 3 mins by fluorescence assay
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[PMID: 23241029] |
| HEK293 | IC50 |
38.4 μM
Compound: prazosin
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Inhibition of human MATE2K-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay
Inhibition of human MATE2K-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay
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[PMID: 23241029] |
| HEK293 | IC50 |
9.9 μM
Compound: prazosin
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Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy
Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy
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[PMID: 18788725] |
| LNCaP | IC50 |
67.46 μM
Compound: 1
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Antiproliferative activity was determined by colorimetric MTS assay in LNCaP cells after 72 hr of incubation with compounds (1-100 uM)
Antiproliferative activity was determined by colorimetric MTS assay in LNCaP cells after 72 hr of incubation with compounds (1-100 uM)
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[PMID: 15633998] |
| PC-3 | IC50 |
11.72 μM
Compound: Prazosin
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Antiproliferative activity against human PC-3 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK8 assay
Antiproliferative activity against human PC-3 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK8 assay
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[PMID: 33160761] |
| PC-3 | IC50 |
11.72 mM
Compound: Prazosin
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Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth
Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth
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[PMID: 34411892] |
Prazosin (50 μM, 18 h) can reduce the production of amyloid beta in N2a cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:N2asw
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Concentration:50 μM
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Incubation Time:18 h
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Result:Reduced Aβ levels, induced a significant increase in APP-CTFs, and significantly reduced the production of CTFγ.
Prazosin (0.1-2 mg/kg, 3-6 mM, administered systemically or locally, in a single dose) can promote fear extinction in mice[3].
Prazosin (0.01-1 mg/kg, subcutaneously, in a single dose) can enhance the analgesic effects of morphine in mice[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:APP23 mice[2]
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Dosage:1 mg/kg; daily; 2 weeks
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Administration:Intraperitoneal injection (i.p.)
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Result:Increased mean freezing time during acclimation, attenuated hippocampal-dependent memory decline, did not affect APP processing or Aβ aggregation in vivo, and increased the number of astrocytes and secretion of anti-inflammatory mediators in APP23 mice.
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Animal Model:C57B1/6N mouse[3]
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Dosage:0.1-2 mg/kg; 3 and 6 mM; single dose
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Administration:systemic (0.1-2 mg/kg) or local microinjections (3 or 6 mM) into the prelimbic division of medial prefrontal cortex or basolateral amygdala
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Result:Had no effect on fear memory, but indirectly promoted fear extinction by interfering with the initial fear acquisition process.
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Animal Model:C57BL/6J mice[4]
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Dosage:0.01, 0.1, 0.25, 0.5, 1 mg/kg; single dose
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Administration:Subcutaneous injection (s.c.)
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Result:Had no analgesic effect when used alone, but it enhanced the analgesic effect of morphine and reduced weight loss after morphine withdrawal.
Chemical Information
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CAS No. 19216-56-9
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Appearance Solid
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Molecular Weight 383.40
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Formula C19H21N5O4
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Color White to off-white
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SMILES
O=C(N1CCN(C2=NC(N)=C3C=C(OC)C(OC)=CC3=N2)CC1)C4=CC=CO4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (18)
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Journal Impact Factor
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Most Recent
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Nature
2025 Dec;648(8092):146-156. PMID: 41094146 -
Cancer Discov
Myofibroblasts Induce Neuroplasticity to Promote Pancreatic Inflammation and Cancer Progression. [Abstract]2026 Feb 9. PMID: 41661076 -
Cell Discov
2023 Feb 7;9(1):16. PMID: 36746933 -
Cell Rep Med
The sympathetic nervous system drives hyperinflammatory responses to Clostridioides difficile infection. [Abstract]2024 Oct 1:101771. PMID: 39368481 -
J Exp Med
2023 Nov 6;220(11):e20230577. PMID: 37584653 -
J Transl Med
CDK1 serves as a therapeutic target of adrenocortical carcinoma via regulating epithelial-mesenchymal transition, G2/M phase transition, and PANoptosis. [Abstract]2022 Oct 2;20(1):444. PMID: 36184616 -
J Med Chem
Development of an In Silico Prediction Model for P-glycoprotein Efflux Potential in Brain Capillary Endothelial Cells toward the Prediction of Brain Penetration. [Abstract]2021 Mar 11;64(5):2725-2738. PMID: 33619967 -
Redox Rep
Norepinephrine promotes oxidative stress in vascular adventitial fibroblasts via PKC/NFκB-mediated NOX2 upregulation. [Abstract]2025 Dec;30(1):2494314. PMID: 40269356
Prazosin purchased from MedChemExpress. Usage Cited in: Redox Rep. 2025 Dec;30(1):2494314. [Abstract]
Prazosin (10 μM) abolished NE-induced increases in NOX2 expression in VAFs of Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR).
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Mol Metab
Vagal control of the brain-esophagus axis ameliorates stress-induced esophageal motility dysfunction in male mice. [Abstract]2026 May 26:102384. PMID: 42203107 -
Drug Des Devel Ther
Dexmedetomidine Prolongs Lidocaine Intravenous Regional Anesthesia in Rats by Blocking the Hyperpolarization-Activated Cation Current. [Abstract]2024 Apr 9:18:1103-1114. PMID: 38618283 -
Cells
Impact of Short-Term (+)-JQ1 Exposure on Mouse Aorta: Unanticipated Inhibition of Smooth Muscle Contractility. [Abstract]2023 May 24;12(11):1461. PMID: 37296583 -
Am J Pathol
Hyperglycemia induces tear reduction and dry eye in diabetic mice through the norepinephrine-α1AR -mitochondrial impairment axis of lacrimal gland. [Abstract]2023 Jul;193(7):913-926. PMID: 37088455 -
iScience
Stimulation of TRPV1+ peripheral somatosensory nerves suppress inflammation via the somato-autonomic reflex. [Abstract]2025 Jan 17;28(2):111831. PMID: 39967868 -
Am J Physiol Renal Physiol
Anatomic and functional evidence for renal autonomic innervation in normotensive and hypertensive rats. [Abstract]2024 Nov 1;327(5):F885-F898. PMID: 39298550 -
BMC Pulm Med
Opposing responses of the rat pulmonary artery and vein to phenylephrine and other agents in vitro. [Abstract]2021 Jun 5;21(1):189. PMID: 34090386
Prazosin purchased from MedChemExpress. Usage Cited in: BMC Pulm Med. 2021 Jun 5;21(1):189. [Abstract]
Prazosin (2 mg/kg; i.p.; 60 min) abolished the inhibition of PAVA on TNF-α production in C57BL/6J male mice.
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Biomed Pharmacother
Prazosin inhibits the proliferation and survival of acute myeloid leukaemia cells through down-regulating TNS1. [Abstract]2020 Apr;124:109731. PMID: 31954876
Prazosin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Apr;124:109731. [Abstract]
The expression of TNS1 in U937 and HL60 cells treated with different concentration of Prazosin is detected by Western blot analysis
Prazosin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Apr;124:109731. [Abstract]
Prazosin (5-15 μM) promoted apoptosis in HL60 and U937 cells in a dose-dependent manner under serum deprivation.
Prazosin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Apr;124:109731. [Abstract]
Cell proliferation was measured by the CCK-8 assay in U937 and HL60 cells treated with Prazosin (5-15 μM).
Prazosin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Apr;124:109731. [Abstract]
Prazosin (15 μM; 24 h) down-regulated the expression of all candidate genes except ABCD1 in U937 cells, with TNS1 being the most significantly down-regulated.
Solvent & Solubility
DMSO : 41.67 mg/mL (108.69 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (13.04 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Day HE, et al. Distribution of alpha 1a-, alpha 1b- and alpha 1d-adrenergic receptor mRNA in the rat brain and spinal cord. J Chem Neuroanat. 1997 Jul;13(2):115-39. [Content Brief]
[2]. Loukia Katsouri, et al. Prazosin, an α(1)-adrenoceptor antagonist, prevents memory deterioration in the APP23 transgenic mouse model of Alzheimer's disease. Neurobiol Aging. 2013 Apr;34(4):1105-15. [Content Brief]
[3]. Elizabeth K Lucas, et al. Prazosin during fear conditioning facilitates subsequent extinction in male C57Bl/6N mice. Psychopharmacology (Berl). 2019 Jan;236(1):273-279. [Content Brief]
[4]. Umit Kazim Ozdoğan, et al. Influence of prazosin and clonidine on morphine analgesia, tolerance and withdrawal in mice. Eur J Pharmacol. 2003 Jan 24;460(2-3):127-34. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6082 mL | 13.0412 mL | 26.0824 mL | 65.2061 mL |
| 5 mM | 0.5216 mL | 2.6082 mL | 5.2165 mL | 13.0412 mL | |
| 10 mM | 0.2608 mL | 1.3041 mL | 2.6082 mL | 6.5206 mL | |
| 15 mM | 0.1739 mL | 0.8694 mL | 1.7388 mL | 4.3471 mL | |
| 20 mM | 0.1304 mL | 0.6521 mL | 1.3041 mL | 3.2603 mL | |
| 25 mM | 0.1043 mL | 0.5216 mL | 1.0433 mL | 2.6082 mL | |
| 30 mM | 0.0869 mL | 0.4347 mL | 0.8694 mL | 2.1735 mL | |
| 40 mM | 0.0652 mL | 0.3260 mL | 0.6521 mL | 1.6302 mL | |
| 50 mM | 0.0522 mL | 0.2608 mL | 0.5216 mL | 1.3041 mL | |
| 60 mM | 0.0435 mL | 0.2174 mL | 0.4347 mL | 1.0868 mL | |
| 80 mM | 0.0326 mL | 0.1630 mL | 0.3260 mL | 0.8151 mL | |
| 100 mM | 0.0261 mL | 0.1304 mL | 0.2608 mL | 0.6521 mL |
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