Cat. No.: HY-12755

ML141

(Synonyms: CID-2950007)

35 Cited Publications

Customer Review

Based on 35 publication(s) in Google Scholar

ML141 (CID-2950007) is a potent, allosteric, selective and reversible non-competitive inhibitor of Cdc42 GTPase. ML141 inhibits Cdc42 wild type and Cdc42 Q61L mutant with EC₅₀s of 2.1 and 2.6 μM, respectively. ML141 shows low micromolar potency and selectivity against other members of the Rho family of GTPases (Rac1, Rab2, Rab7). ML141 do not show cytotoxicity in multiple cell lines.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 71203-35-5
Formula: C₂₂H₂₁N₃O₃S
Molecular Weight:407.49

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

ML141 Chemical Structure

CAS No.: 71203-35-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (35)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Ras Isoforms :

In Vitro

ML141 (CID-2950007) is not cytotoxic in either cell line at doses of 0.1-3 μM after treatment for 4 days. OVCA429 cells were insensitive to 10 μM compound, whereas some cytotoxicity was observed in SKOV3ip cells at this concentration after a 4-day treatment, although it did not reach statistical significance. ML141 is not cytotoxic toward Swiss 3T3 or Vero E6 cells up to 10 μM for 24 and 48 h, respectively^[1].
ML141 inhibits 3T3 fibroblast filopodia formation and inhibits ovarian cancer cell migration^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

ML141 (CID-2950007) (10 μM; intracerebroventricular injection) causes acute anxiety in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57Bl/6J mice^[3]
Dosage:

10 µM
Administration:

Intracerebroventricular injection
Result:

Increased anxiety in mice.

Chemical Information

CAS No. 71203-35-5
Appearance Solid
Molecular Weight 407.49
Formula C₂₂H₂₁N₃O₃S
Color White to off-white
SMILES

O=S(C1=CC=C(N2N=C(C3=CC=CC=C3)CC2C4=CC=C(OC)C=C4)C=C1)(N)=O
Synonyms

CID-2950007
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (35)

All IFIHCCell Imaging/StainingBio/Physico-chemical Assay

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Journal Impact Factor

Immunity

Membrane integrity changes upon viral infection activate sphingomyelinase SMPDL3B to restrict cGAS-STING signaling via cGAMP degradation. [Abstract]

2025 Nov 11;58(11):2670-2684.e10. PMID: 41175872
Sci Bull

Single particle tracking reveals SARS-CoV-2 regulating and utilizing dynamic filopodia for viral invasion. [Abstract]

2023 Oct 15;68(19):2210-2224. PMID: 37661543
Adv Funct Mater

2024 May 1.
Nat Commun

Harnessing macrophage-drug conjugates for allogeneic cell-based therapy of solid tumors via the TRAIN mechanism. [Abstract]

2025 Feb 4;16(1):1327. PMID: 39900573
Nat Commun

Salmonella effector SopB reorganizes cytoskeletal vimentin to maintain replication vacuoles for efficient infection. [Abstract]

2023 Jan 30;14(1):478. PMID: 36717589

ML141 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jan 30;14(1):478. [Abstract]

Treatment with Cdc42 inhibitor ML141 (5 µM) disassembled SCVs giving rise to increased proportion of dispersive SCV in wild-type, but not in vimentin KO cells.
Nat Commun

pTINCR microprotein promotes epithelial differentiation and suppresses tumor growth through CDC42 SUMOylation and activation. [Abstract]

2022 Nov 11;13(1):6840. PMID: 36369429
Genes Dis

2026 Jan 21.
Adv Sci (Weinh)

Low YTHDC1 Expression Upregulates FSCN1 to Promote Nuclear F-Actin Formation and Facilitate Double-strand DNA Breaks Repair in TMZ-Resistant Glioblastoma. [Abstract]

2025 Dec 27:e13632. PMID: 41454694
Cell Death Dis

Pictilisib and nutrient stress synergize to induce methuosis via PI(4,5)P2-dependent macropinocytic dysregulation in cancer cells. [Abstract]

2026 Jun 16. PMID: 42303628
Cell Death Dis

cGAS-STING/HMGB1-mediated senescence induced by LRRK2 accelerates cartilage degeneration in osteoarthritis. [Abstract]

2026 Mar 25;17(1):377. PMID: 41881967
Angiogenesis

MY-1 promotes angiogenesis in the ischemic hindlimbs by regulating the stability of CDC42 via PSMD14. [Abstract]

2025 Aug 20;28(4):44. PMID: 40833626

ML141 purchased from MedChemExpress. Usage Cited in: Angiogenesis. 2025 Aug 20;28(4):44. [Abstract]

In the MY-1 group, cells at the wound edge extended filopodia in the direction of migration, while in the presence of ML141, filopodia formation was suppressed, and MY-1 could not reverse the inhibitory effect of ML141 (10 μM).

ML141 purchased from MedChemExpress. Usage Cited in: Angiogenesis. 2025 Aug 20;28(4):44. [Abstract]

Treatment with ML141 reduced the primary tube length, node count, network formation in the tube formation assay and the number of migrated cells in the Transwell assay. Similarly, MY-1 could not reverse the inhibitory effect of ML141(10 μM).
Br J Cancer

PAK and PI3K pathway activation confers resistance to KRASG12C inhibitor sotorasib. [Abstract]

2023 Jan;128(1):148-159. PMID: 36319849
Stem Cell Res Ther

Exosomes derived from stem cells from apical papilla promote craniofacial soft tissue regeneration by enhancing Cdc42-mediated vascularization. [Abstract]

2021 Jan 22;12(1):76. PMID: 33482924
Cell Rep

Context-dependent inhibitory roles of RhoA in 3D invasive cell migration within the extracellular matrix. [Abstract]

2025 Dec 6;44(12):116649. PMID: 41359424
Cell Rep

ENKD1 modulates innate immune responses through enhanced geranylgeranyl pyrophosphate synthase activity. [Abstract]

2025 Mar 5;44(3):115397. PMID: 40048432
Int J Oncol

LIS1 interacts with CLIP170 to promote tumor growth and metastasis via the Cdc42 signaling pathway in salivary gland adenoid cystic carcinoma. [Abstract]

2022 Oct;61(4):129. PMID: 36102310
Hum Reprod

CDC42 deficiency leads to endometrial stromal cell senescence in recurrent implantation failure. [Abstract]

2024 Dec 1;39(12):2768-2784. PMID: 39487595

ML141 purchased from MedChemExpress. Usage Cited in: Hum Reprod. 2024 Dec 1;39(12):2768-2784. [Abstract]

ML141 (10 μM)-treated primary EnSCs remained spindle-shaped fibroblast-like cells after decidualization stimulation, without an obvious change in cell size

ML141 purchased from MedChemExpress. Usage Cited in: Hum Reprod. 2024 Dec 1;39(12):2768-2784. [Abstract]

ML141 (10μM) treatment hindered the upregulation of sST2 without affecting the secretion of CLU.
Cell Biosci

Hypofucosylation of Unc5b regulated by Fut8 enhances macrophage emigration and prevents atherosclerosis. [Abstract]

2023 Jan 20;13(1):13. PMID: 36670464
Int Endod J

A Wet-and-Dry Research Model: Unveiling Biomechanics in Odontoblast Polarization. [Abstract]

2026 Jun 2. PMID: 42227345
Glia

Celsr2-mediated morphological polarization and functional phenotype of reactive astrocytes in neural repair. [Abstract]

2023 Aug;71(8):1985-2004. PMID: 37186402
Sci Rep

TRPV4 activates the Cdc42/N-wasp pathway to promote glioblastoma invasion by altering cellular protrusions. [Abstract]

2020 Aug 25;10(1):14151. PMID: 32843668
Comput Struct Biotechnol J

Dependence of SARS-CoV-2 infection on cholesterol-rich lipid raft and endosomal acidification. [Abstract]

2021:19:1933-1943. PMID: 33850607
J Virol

In vivo investigation of PEDV transmission via nasal infection: mechanisms of CD4+ T-cell-mediated intestinal infection. [Abstract]

2025 Apr 15;99(4):e0176124. PMID: 40094365
PLoS Genet

Juvenile hormone promotes paracellular transport of yolk proteins via remodeling zonula adherens at tricellular junctions in the follicular epithelium. [Abstract]

2022 Jun 27;18(6):e1010292. PMID: 35759519
Cell Mol Bioeng

Light Activates Cdc42-Mediated Needle-Shaped Filopodia Formation via the Integration of Small GTPases. [Abstract]

2022 Oct 6;15(6):599-609. PMID: 36531863
Vet Microbiol

Epidermal growth factor receptor (EGFR) promotes uptake of bovine parainfluenza virus type 3 into MDBK cells. [Abstract]

2022 Aug:271:109488. PMID: 35691094
Virology

The actin cytoskeleton is important for pseudorabies virus infection. [Abstract]

2024 Dec:600:110233. PMID: 39255726
Biochem Biophys Res Commun

Cdc42-mediated supracellular cytoskeleton induced cancer cell migration under low shear stress. [Abstract]

2019 Oct 29;519(1):134-140. PMID: 31477271
Oncol Lett

EGFR and ERK activation resists flavonoid quercetin-induced anticancer activities in human cervical cancer cells in vitro. [Abstract]

2021 Nov;22(5):754. PMID: 34539858
Res Sq

2026 Apr 26.
bioRxiv

2025 January 14.
bioRxiv

2024 October 12.
medRxiv

2023 Nov 11.
bioRxiv

2023 Oct 16.
Indian J Pharm Sci

2021 Jun.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (245.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4540 mL

12.2702 mL

24.5405 mL

5 mM

0.4908 mL

2.4540 mL

4.9081 mL

10 mM

0.2454 mL

1.2270 mL

2.4540 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.08 mg/mL (5.10 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (5.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hong L, et al. Characterization of a Cdc42 protein inhibitor and its use as a molecular probe. J Biol Chem. 2013 Mar 22;288(12):8531-43. [Content Brief]

[2]. Surviladze Z, et al. A Potent and Selective Inhibitor of Cdc42 GTPase. Probe Reports from the NIH Molecular Libraries Program [Content Brief]

[3]. Hanin G, et al. Competing targets of microRNA-608 affect anxiety and hypertension. Hum Mol Genet. 2014 Sep 1;23(17):4569-80. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4540 mL	12.2702 mL	24.5405 mL	61.3512 mL
	5 mM	0.4908 mL	2.4540 mL	4.9081 mL	12.2702 mL
	10 mM	0.2454 mL	1.2270 mL	2.4540 mL	6.1351 mL
	15 mM	0.1636 mL	0.8180 mL	1.6360 mL	4.0901 mL
	20 mM	0.1227 mL	0.6135 mL	1.2270 mL	3.0676 mL
	25 mM	0.0982 mL	0.4908 mL	0.9816 mL	2.4540 mL
	30 mM	0.0818 mL	0.4090 mL	0.8180 mL	2.0450 mL
	40 mM	0.0614 mL	0.3068 mL	0.6135 mL	1.5338 mL
	50 mM	0.0491 mL	0.2454 mL	0.4908 mL	1.2270 mL
	60 mM	0.0409 mL	0.2045 mL	0.4090 mL	1.0225 mL
	80 mM	0.0307 mL	0.1534 mL	0.3068 mL	0.7669 mL
	100 mM	0.0245 mL	0.1227 mL	0.2454 mL	0.6135 mL

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ML141

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Cell Death Library

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Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Lactylation Compound Library

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GPCR/G Protein Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

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Peptidomimetic Library

Publications Citing Use of MedChemExpress (MCE) ML141

All Ras Isoforms

Biological Activity

Chemical Information

Publications (35)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

ML141 Related Classifications

HY-12755 Related Classifications