Nimodipine
Based on 21 publication(s) in Google Scholar
Nimodipine (BAY-e 9736) is an orally active, well-tolerated and light-sensitive dihydropyridine calcium antagonist. Nimodipine can be used for the research of cerebrovascular disorders.
For research use only. We do not sell to patients.
- Purity: 99.86%
- CAS No.: 66085-59-4
- Formula: C21H26N2O7
- Molecular Weight:418.44
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Nimodipine
More- Adv Healthc Mater. 2022 Jun;11(11):e2102256. [Abstract]
- Proc Natl Acad Sci U S A. 2026 Mar 31;123(13):e2520119123. [Abstract]
- Phytomedicine. 2025 May:140:156623. [Abstract]
- Aging Cell. 2024 Aug 15:e14305. [Abstract]
- Antioxid Redox Signal. 2025 Sep 17. [Abstract]
- J Ethnopharmacol. 2025 Jun 13:120147. [Abstract]
- Front Pharmacol. 2021 Sep 13:12:733420. [Abstract]
- Int Immunopharmacol. 2025 Dec 8:169:115957. [Abstract]
- J Med Virol. 2025 May;97(5):e70393. [Abstract]
- J Physiol. 2022 Aug;600(16):3775-3793. [Abstract]
- Eur J Cell Biol. 2024 May 28;103(2):151427. [Abstract]
- Front Cell Neurosci. 2020 Oct 16;14:575626. [Abstract]
- ACS Chem Neurosci. 2025 Apr 28. [Abstract]
- Mol Med Rep. 2020 Dec;22(6):5135-5144. [Abstract]
- Chem Biol Drug Des. 2025 Mar;105(3):e70083. [Abstract]
- Front Neural Circuits. 2021 Apr 6:15:657445. [Abstract]
- Neuroscience. 2022 Jun 1;492:47-57. [Abstract]
- Neurosci Res. 2025 Dec 13:223:105008. [Abstract]
- J Neurophysiol. 2020 Jan 1;123(1):277-288. [Abstract]
- J Stroke Cerebrovasc Dis. 2024 Jun;33(6):107689. [Abstract]
- SSRN. 2023 Sep 7.
Customer Validation & Images
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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Histological Imaging/Staining
All Calcium Channel Isoforms
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Biological Activity
dihydropyridine calcium[1]
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Cell Line
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Type | Value | Description | References |
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| CHO | EC50 |
0.8 μM
Compound: 54
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Agonist activity at mouse TRPA1 channel expressed in CHO cells assessed as increase in intracellular calcium influx
Agonist activity at mouse TRPA1 channel expressed in CHO cells assessed as increase in intracellular calcium influx
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[PMID: 20356305] |
| HEK293 | IC50 |
0.11 μM
Compound: Nimodipine
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Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit
Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit
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[PMID: 22761000] |
| HepG2 | EC50 |
0.39 μM
Compound: 5
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Agonist activity at PXR (unknown origin) expressed in human HepG2 cells assessed as induction of CYP3A4 transactivation after 16 hrs by luciferase reporter gene assay
Agonist activity at PXR (unknown origin) expressed in human HepG2 cells assessed as induction of CYP3A4 transactivation after 16 hrs by luciferase reporter gene assay
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[PMID: 23688559] |
| LLC-MK2 | IC50 |
32.31 μM
Compound: Nimodipine
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Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi infected in rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay
Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi infected in rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay
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[PMID: 20934347] |
| LLC-MK2 | IC50 |
96.19 μM
Compound: Nimodipine
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Cytotoxicity against rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay
Cytotoxicity against rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay
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[PMID: 20934347] |
| PC-12 | EC50 |
1.29 μg/mL
Compound: Nimodipine
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Anti-anoxic activity in rat PC12 cells reduction in assessed as sodium hydrosulfite-induced cell injury
Anti-anoxic activity in rat PC12 cells reduction in assessed as sodium hydrosulfite-induced cell injury
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[PMID: 31317731] |
| PC-12 | EC50 |
97.3 μM
Compound: Nimodipine
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Neuroprotective activity against glutamate-induced neuronal cell death in rat PC12 cells assessed as increase in cell viability pretreated for 2 hrs followed by glutamate challenge measured after 24 hrs by MTT assay
Neuroprotective activity against glutamate-induced neuronal cell death in rat PC12 cells assessed as increase in cell viability pretreated for 2 hrs followed by glutamate challenge measured after 24 hrs by MTT assay
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[PMID: 29775304] |
| SH-SY5Y | IC50 |
14 μM
Compound: Nimodipine
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Inhibition of K+-induced voltage gated calcium channel opening in human SH-SY5Y cells assessed as decrease in Ca2+ level after 10 mins by Fluo-4 dye-based fluorescence assay
Inhibition of K+-induced voltage gated calcium channel opening in human SH-SY5Y cells assessed as decrease in Ca2+ level after 10 mins by Fluo-4 dye-based fluorescence assay
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[PMID: 30530190] |
| Ventricular myocyte | IC50 |
0.3 μM
Compound: Nimodipine
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Inhibition of L-type calcium channel measured using whole-cell patch clamp in guinea pig ventricular myocytes
Inhibition of L-type calcium channel measured using whole-cell patch clamp in guinea pig ventricular myocytes
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[PMID: 22761000] |
Nimodipine (1.5~150 μg/ml; 15 minutes; B16a and W256 cells) results in a dose-dependent inhibition of B16a and W256 tumor-cell-induced platelet aggregation. Nimodipine is also inhibitory in a homologous system[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Nimodipine(0.1~80 mg/kg; p.o.) results in a significant dose-dependent inhibition of spontaneous metastasis[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:New Zealand white rabbits[2]
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Dosage:0.2 µg/µl
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Administration:Intrathecal administration
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Result:Prevented subarachnoid hemorrhage-associated cerebral vasospasm by prophylactic continuous intrathecal administration.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 66085-59-4
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Appearance Solid
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Molecular Weight 418.44
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Formula C21H26N2O7
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Color Light yellow to yellow
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SMILES
O=C(C1=C(C)NC(C)=C(C(OC(C)C)=O)C1C2=CC=CC([N+]([O-])=O)=C2)OCCOC
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Synonyms
BAY-e 9736
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (21)
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Journal Impact Factor
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Most Recent
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Adv Healthc Mater
A Novel Targeted Nanoparticle for Traumatic Brain Injury Treatment: Combined Effect of ROS Depletion and Calcium Overload Inhibition. [Abstract]2022 Jun;11(11):e2102256. PMID: 35118827 -
Proc Natl Acad Sci U S A
Endogenous ATP-powered nanomotors directing neural stem cell differentiation for Parkinson's disease treatment. [Abstract]2026 Mar 31;123(13):e2520119123. PMID: 41880568 -
Phytomedicine
Shenqi granules enhance recovery from cerebral ischemia-reperfusion injury by modulating tryptophan and tyrosine metabolism and activating NFE2L2/NRF2. [Abstract]2025 May:140:156623. PMID: 40068292 -
Aging Cell
Upregulation of the Cav1.3 channel in inner hair cells by interleukin 6-dependent inflammaging contributes to age-related hearing loss. [Abstract]2024 Aug 15:e14305. PMID: 39148148
Nimodipine purchased from MedChemExpress. Usage Cited in: Aging Cell. 2024 Aug 15:e14305. [Abstract]
Timeline for ABR testing and drug treatment. Nimodipine was administered subcutaneously to 7-month-old WT mice at a dose of 5 mg/kg or 10 mg/kg every other day for 60 days.
Nimodipine purchased from MedChemExpress. Usage Cited in: Aging Cell. 2024 Aug 15:e14305. [Abstract]
Timeline for ABR testing and drug treatment. Nimodipine was administered subcutaneously to 7-month-old WT mice at a dose of 5 mg/kg or 10 mg/kg every other day for 60 days. Representative ABR waveforms in response to click stimuli (90-30 dB) in mice.
Nimodipine purchased from MedChemExpress. Usage Cited in: Aging Cell. 2024 Aug 15:e14305. [Abstract]
Timeline for ABR testing and drug treatment. Nimodipine was administered subcutaneously to 7-month-old WT mice at a dose of 5 mg/kg or 10 mg/kg every other day for 60 days. ABR threshold to click stimuli in the AHL, solvent, and Nimodipine (5, 10 mg/kg) treatment groups.
Nimodipine purchased from MedChemExpress. Usage Cited in: Aging Cell. 2024 Aug 15:e14305. [Abstract]
Timeline for ABR testing and drug treatment. Nimodipine was administered subcutaneously to 7-month-old WT mice at a dose of 5 mg/kg or 10 mg/kg every other day for 60 days. ABR threshold to pure-tone in the AHL, solvent, and Nimodipine (5, 10 mg/kg) treatment groups.
Nimodipine purchased from MedChemExpress. Usage Cited in: Aging Cell. 2024 Aug 15:e14305. [Abstract]
Immunofluorescence of IHCs and ribbon synapses in the apex, middle, and base of cochlea from WT 9 M mice, solvent-treated, and Nimodipine (5, 10 mg/kg)-treated mice.
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Antioxid Redox Signal
Nimodipine Blocks Histone-Induced Calcium Overload to Protect Neurons after Traumatic Brain Injury. [Abstract]2025 Sep 17. PMID: 40960921 -
J Ethnopharmacol
Harpagide from radix scrophulariae attenuated ischemic stroke injury through calcium homeostasis regulation between mitochondria-associated membranes via the IP3R1/GRP75/VDAC1 complex. [Abstract]2025 Jun 13:120147. PMID: 40517916 -
Front Pharmacol
2021 Sep 13:12:733420. PMID: 34594224 -
Int Immunopharmacol
Shikonin improves cerebral ischemia-reperfusion injury by regulating astrocyte polarization through ERK1/2-SP1-SLC7A10. [Abstract]2025 Dec 8:169:115957. PMID: 41365200 -
J Med Virol
Influenza A Virus Induces Autophagosome by Inhibiting LTCC/Calpain 2/LC3A Signaling to Promote Viral Replication. [Abstract]2025 May;97(5):e70393. PMID: 40358023 -
J Physiol
Chronic exercise increases excitability of lamina X neurons through enhancement of persistent inward currents and dendritic development in mice. [Abstract]2022 Aug;600(16):3775-3793. PMID: 35848453 -
Eur J Cell Biol
The mechanical mechanism of angiotensin II induced activation of hepatic stellate cells promoting portal hypertension. [Abstract]2024 May 28;103(2):151427. PMID: 38820882 -
Front Cell Neurosci
Three-Week Treadmill Exercise Enhances Persistent Inward Currents, Facilitates Dendritic Plasticity, and Upregulates the Excitability of Dorsal Raphe Serotonin Neurons in ePet-EYFP Mice. [Abstract]2020 Oct 16;14:575626. PMID: 33177992 -
ACS Chem Neurosci
Poliumoside Exhibits Neuroprotective Effects against Cerebral Ischemia-Reperfusion Injury by Relieving Microglia-Mediated Neuronal Damage and Astrocytic Activation. [Abstract]2025 Apr 28. PMID: 40295176 -
Mol Med Rep
Eupafolin alleviates cerebral ischemia/reperfusion injury in rats via blocking the TLR4/NF‑κB signaling pathway. [Abstract]2020 Dec;22(6):5135-5144. PMID: 33173992 -
Chem Biol Drug Des
Forsythiaside A Ameliorates Oxidative Damage Caused by Cerebral Ischemia Through the Nrf2/HO-1 Signaling Pathway. [Abstract]2025 Mar;105(3):e70083. PMID: 40035314 -
Front Neural Circuits
Serotonergic Modulation of Persistent Inward Currents in Serotonergic Neurons of Medulla in ePet-EYFP Mice. [Abstract]2021 Apr 6:15:657445. PMID: 33889077 -
Neuroscience
TGF-β1 Protects Trauma-injured Murine Cortical Neurons by Upregulating L-type Calcium Channel Cav1.2 via the p38 Pathway. [Abstract]2022 Jun 1;492:47-57. PMID: 35460836 -
Neurosci Res
Inhibition of calcium-sensing receptors improves intestinal motility in obese mice through enteric neurons and vagal center. [Abstract]2025 Dec 13:223:105008. PMID: 41397659 -
J Neurophysiol
Sequential activation of multiple persistent inward currents induces staircase currents in serotonergic neurons of medulla in ePet-EYFP mice. [Abstract]2020 Jan 1;123(1):277-288. PMID: 31721638 -
J Stroke Cerebrovasc Dis
Shikonin attenuates cerebral ischemia/reperfusion injury via inhibiting NOD2/RIP2/NF-κB-mediated microglia polarization and neuroinflammation. [Abstract]2024 Jun;33(6):107689. PMID: 38527567 -
Solvent & Solubility
DMSO : 100 mg/mL (238.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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-
-
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Langley, M.S., et al. Nimodipine. Drugs 37, 669–699 (1989).
[2]. Marbacher S, et al. Prevention of delayed cerebral vasospasm by continuous intrathecal infusion of glyceroltrinitrate and nimodipine in the rabbit model in vivo. Intensive Care Med. 2008;34(5):932-938. [Content Brief]
[3]. Honn KV, et al. Inhibition of tumor cell-platelet interactions and tumor metastasis by the calcium channel blocker, nimodipine. Clin Exp Metastasis. 1984;2(1):61-72. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3898 mL | 11.9491 mL | 23.8983 mL | 59.7457 mL |
| 5 mM | 0.4780 mL | 2.3898 mL | 4.7797 mL | 11.9491 mL | |
| 10 mM | 0.2390 mL | 1.1949 mL | 2.3898 mL | 5.9746 mL | |
| 15 mM | 0.1593 mL | 0.7966 mL | 1.5932 mL | 3.9830 mL | |
| 20 mM | 0.1195 mL | 0.5975 mL | 1.1949 mL | 2.9873 mL | |
| 25 mM | 0.0956 mL | 0.4780 mL | 0.9559 mL | 2.3898 mL | |
| 30 mM | 0.0797 mL | 0.3983 mL | 0.7966 mL | 1.9915 mL | |
| 40 mM | 0.0597 mL | 0.2987 mL | 0.5975 mL | 1.4936 mL | |
| 50 mM | 0.0478 mL | 0.2390 mL | 0.4780 mL | 1.1949 mL | |
| 60 mM | 0.0398 mL | 0.1992 mL | 0.3983 mL | 0.9958 mL | |
| 80 mM | 0.0299 mL | 0.1494 mL | 0.2987 mL | 0.7468 mL | |
| 100 mM | 0.0239 mL | 0.1195 mL | 0.2390 mL | 0.5975 mL |
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