DNMT2-IN-2
Based on 1 Customer Validation
DNMT2-IN-2 is a selective DNA methyltransferase 2 (DNMT2) inhibitor with a KD value of 3.04 μM. DNMT2-IN-2 targets to a cryptic allosteric binding site of DNMT2. DNMT2-IN-2 reduces m5C levels in MOLM-13 tRNA and synergizes with Doxorubicin (HY-15142A) to impair cell viability. DNMT2-IN-2 can be used for cancer research, such as cervical cancer and leukemia.
For research use only. We do not sell to patients.
- Purity: 99.13%
- Formula: C26H31ClN4O4
- Molecular Weight:499.00
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All DNA Methyltransferase Isoforms
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Biological Activity
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DNMT2 3.04 μM (Kd) |
DNMT2-IN-2 (Compound 16) (50 μM, 72 h) significantly reduces levels of targeted tRNA modifications (m5C and m5Cm) in MOLM-13 cells[1].
DNMT2-IN-2 (50 μM, 16 h) shows thermal stabilization of DNMT2 in MOLM-13 cells[1].
DNMT2-IN-2 (1-100 μM, 48 h) shows low toxicity in HCT 116, MOLM13, HEK293, MOLM-13 and Huh7 cells[1].
DNMT2-IN-2 (50 μM, 6 days) makes cells more susceptible to Doxorubicin (HY-15142A) in MOLM-13, Huh-7 and MDA-MB-231 cells[1].
DNMT2-IN-2 (50 μM, 45 mins) does not display NonO nor eIF4E from binding to immobilized DNMT2[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MOLM-13 cells
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Concentration:50 μM
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Incubation Time:
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Result:Showed thermal stabilization of DNMT2.
Chemical Information
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Appearance Solid
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Molecular Weight 499.00
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Formula C26H31ClN4O4
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Color White to off-white
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SMILES
NC(CNC(C[C@H](NC(C1(CC1)CNC(C2=CC(CC)=CC=C2)=O)=O)CC3=CC=C(C=C3)Cl)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (200.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.0040 mL | 10.0200 mL | 20.0401 mL | 50.1002 mL |
| 5 mM | 0.4008 mL | 2.0040 mL | 4.0080 mL | 10.0200 mL | |
| 10 mM | 0.2004 mL | 1.0020 mL | 2.0040 mL | 5.0100 mL | |
| 15 mM | 0.1336 mL | 0.6680 mL | 1.3360 mL | 3.3400 mL | |
| 20 mM | 0.1002 mL | 0.5010 mL | 1.0020 mL | 2.5050 mL | |
| 25 mM | 0.0802 mL | 0.4008 mL | 0.8016 mL | 2.0040 mL | |
| 30 mM | 0.0668 mL | 0.3340 mL | 0.6680 mL | 1.6700 mL | |
| 40 mM | 0.0501 mL | 0.2505 mL | 0.5010 mL | 1.2525 mL | |
| 50 mM | 0.0401 mL | 0.2004 mL | 0.4008 mL | 1.0020 mL | |
| 60 mM | 0.0334 mL | 0.1670 mL | 0.3340 mL | 0.8350 mL | |
| 80 mM | 0.0251 mL | 0.1253 mL | 0.2505 mL | 0.6263 mL | |
| 100 mM | 0.0200 mL | 0.1002 mL | 0.2004 mL | 0.5010 mL |
DNMT2-IN-2 Related Classifications
HY-175594 Related Classifications
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