(E)-Osmundacetone
Based on 2 publication(s) in Google Scholar
(E)-Osmundacetone is an inhibitor of the MAPK pathway. (E)-Osmundacetone inhibits the activation of the MAPK signaling pathway and restores the expression of PPARα/ACOX1. (E)-Osmundacetone abrogates abnormal cell proliferation, migration and liver metastasis induced by PTPRO silencing in colorectal cancer cells. (E)-Osmundacetone blocks OA-RD17-mediated activation of the MAPK signaling pathway, thereby reducing macrophage proliferation and migration. (E)-Osmundacetone is applicable to relevant research on colorectal cancer.
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- Pureté: 99.81%
- CAS No.: 123694-03-1
- Formule: C10H10O3
- Masse moléculaire:178.19
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) (E)-Osmundacetone
More
Activité biologique
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PPARα |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>0.28 μM
Compound: 9
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Cytotoxicity against human A549 cells after 96 hrs by MTT assay
Cytotoxicity against human A549 cells after 96 hrs by MTT assay
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[PMID: 15165144] |
| A549 | IC50 |
>10 μM
Compound: 18
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Cytotoxicity against human A549 cells after 4 days by MTT assay
Cytotoxicity against human A549 cells after 4 days by MTT assay
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[PMID: 24359303] |
| Bel-7402 | IC50 |
0.153 μM
Compound: 9
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Cytotoxicity against human Bel7402 cells after 96 hrs by MTT assay
Cytotoxicity against human Bel7402 cells after 96 hrs by MTT assay
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[PMID: 15165144] |
| Bel-7402 | IC50 |
4.7 μM
Compound: 18
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Cytotoxicity against human Bel7402 cells after 4 days by MTT assay
Cytotoxicity against human Bel7402 cells after 4 days by MTT assay
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[PMID: 24359303] |
| BGC-823 | IC50 |
0.243 μM
Compound: 9
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Cytotoxicity against human BGC823 cells after 96 hrs by MTT assay
Cytotoxicity against human BGC823 cells after 96 hrs by MTT assay
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[PMID: 15165144] |
| BMDM | IC50 |
7.8 μM
Compound: 1
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Inhibition of M-CSF/RANKL-induced osteoclast differentiation in C57BL/6 mouse bone marrow macrophage assessed as reduction in multinucleated TRAP+ cells incubated for 6 days with fresh media replacement on day 3 and measured on day 6 by TRAP staining-base
Inhibition of M-CSF/RANKL-induced osteoclast differentiation in C57BL/6 mouse bone marrow macrophage assessed as reduction in multinucleated TRAP+ cells incubated for 6 days with fresh media replacement on day 3 and measured on day 6 by TRAP staining-base
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[PMID: 31257875] |
| HCT-8 | IC50 |
0.227 μM
Compound: 9
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Cytotoxicity against human HCT8 cells after 96 hrs by MTT assay
Cytotoxicity against human HCT8 cells after 96 hrs by MTT assay
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[PMID: 15165144] |
| HT-22 | EC50 |
1.5 μM
Compound: 31
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Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay
Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay
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[PMID: 32991171] |
| KB | IC50 |
>10 μM
Compound: 18
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Cytotoxicity against human KB cells after 4 days by MTT assay
Cytotoxicity against human KB cells after 4 days by MTT assay
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[PMID: 24359303] |
| KETR3 | IC50 |
0.245 μM
Compound: 9
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Cytotoxicity against human Ketr3 cells after 96 hrs by MTT assay
Cytotoxicity against human Ketr3 cells after 96 hrs by MTT assay
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[PMID: 15165144] |
| MCF7 | IC50 |
0.141 μM
Compound: 9
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Cytotoxicity against human MCF7 cells after 96 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 96 hrs by MTT assay
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[PMID: 15165144] |
(E)-osmundacetone (40 μM; 24 h) restores the expression of PPARα/ACOX1, enhances fatty acid oxidation (FAO), and reverses the promotion of proliferation, migration and liver metastasis in PTPRO-silenced SW480 and LoVo colorectal cancer (CRC) cells mediated by PTPRO silencing[1].
(E)-Osmundacetone (10 μM) blocks the enhanced proliferation and migration of RAW264.7 macrophages induced by OA-RD17[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Keratinocytes
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Concentration:Unclear
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Incubation Time:24 h
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Result:Significantly suppressed the up-regulation of miR-632 by OA-RD17 in keratinocytes.
Chemical Information
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CAS No. 123694-03-1
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Appearance Solid
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Masse moléculaire 178.19
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Formule C10H10O3
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Color Light yellow to yellow
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SMILES
CC(/C=C/C1=CC=C(O)C(O)=C1)=O
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Structure Classification
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Initial Source
Inonotus hispidus
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Cancer Commun (Lond)
PTPRO represses colorectal cancer tumorigenesis and progression by reprogramming fatty acid metabolism. [Abstract]2022 Sep;42(9):848-867. PMID: 35904817 -
Cell Mol Biol Lett
2023 Jul 28;28(1):61. PMID: 37501100
Solvant et solubilité
DMSO : 100 mg/mL (561.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (14.03 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (282 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Dai W, et al. PTPRO represses colorectal cancer tumorigenesis and progression by reprogramming fatty acid metabolism. Cancer Commun (Lond). 2022;42(9):848-867. [Content Brief]
[2]. Li C, et al. A frog peptide provides new strategies for the intervention against skin wound healing. Cell Mol Biol Lett. 2023;28(1):61. Published 2023 Jul 28. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.6120 mL | 28.0599 mL | 56.1199 mL | 140.2997 mL |
| 5 mM | 1.1224 mL | 5.6120 mL | 11.2240 mL | 28.0599 mL | |
| 10 mM | 0.5612 mL | 2.8060 mL | 5.6120 mL | 14.0300 mL | |
| 15 mM | 0.3741 mL | 1.8707 mL | 3.7413 mL | 9.3533 mL | |
| 20 mM | 0.2806 mL | 1.4030 mL | 2.8060 mL | 7.0150 mL | |
| 25 mM | 0.2245 mL | 1.1224 mL | 2.2448 mL | 5.6120 mL | |
| 30 mM | 0.1871 mL | 0.9353 mL | 1.8707 mL | 4.6767 mL | |
| 40 mM | 0.1403 mL | 0.7015 mL | 1.4030 mL | 3.5075 mL | |
| 50 mM | 0.1122 mL | 0.5612 mL | 1.1224 mL | 2.8060 mL | |
| 60 mM | 0.0935 mL | 0.4677 mL | 0.9353 mL | 2.3383 mL | |
| 80 mM | 0.0701 mL | 0.3507 mL | 0.7015 mL | 1.7537 mL | |
| 100 mM | 0.0561 mL | 0.2806 mL | 0.5612 mL | 1.4030 mL |
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