Cat. No.: HY-B1776

Spermidine

20 Cited Publications

Customer Review

Based on 20 publication(s) in Google Scholar

Spermidine maintains cell membrane stability, increases antioxidant enzymes activities, improving photosystem II (PSII), and relevant gene expression. Spermidine significantly decreases the H₂O₂ and O₂^.- contents.

For research use only. We do not sell to patients.

Purity: 99.0%
CAS No.: 124-20-9
Formula: C₇H₁₉N₃
Molecular Weight:145.25

Storage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Spermidine Chemical Structure

CAS No.: 124-20-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Publications (20)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	>100 μM Compound: Spd, spermidine	Cytotoxicity against CHO cells after 48 hrs by MTT assay Cytotoxicity against CHO cells after 48 hrs by MTT assay	[PMID: 18558490]
CHO	IC₅₀	>100 μM Compound: Spd, spermidine	Cytotoxicity against polyamine transport-deficient mutant CHO cells after 48 hrs by MTT assay Cytotoxicity against polyamine transport-deficient mutant CHO cells after 48 hrs by MTT assay	[PMID: 18558490]
CHO-MG	IC₅₀	>100 μM Compound: 2, Spermidine	Inhibition of cell growth in CHO-MG cells in the presence of DFMO Inhibition of cell growth in CHO-MG cells in the presence of DFMO	[PMID: 16392808]
HeLa	IC₅₀	100 μM Compound: spermidine	Ability to inhibit 50% growth of HeLa cells Ability to inhibit 50% growth of HeLa cells	[PMID: 1875339]
HeLa	IC₅₀	1500 μM Compound: spermidine	Ability to cause aggregation of purified HeLa cell DNA Ability to cause aggregation of purified HeLa cell DNA	[PMID: 1875339]
L1210	IC₅₀	>100 μM Compound: Spd, spermidine	Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay	[PMID: 18558490]
L1210	IC₅₀	>100 μM Compound: 7	Growth inhibitory activity against L1210 cells (48h) Growth inhibitory activity against L1210 cells (48h)	[PMID: 9154970]
L1210	IC₅₀	>100 μM Compound: 7	Growth inhibitory activity against L1210 cells (96h) Growth inhibitory activity against L1210 cells (96h)	[PMID: 9154970]
RAW264.7	CC₅₀	>100 μM Compound: Spermidine	Cytotoxicity against mouse RAW264.7 cells assessed as cell growth inhibition after 72 hrs by Alamar blue dye-based assay Cytotoxicity against mouse RAW264.7 cells assessed as cell growth inhibition after 72 hrs by Alamar blue dye-based assay	[PMID: 27793448]

In Vitro

Spermidine?plays a crucial role of against abiotic stresses, such as salt, drought, heat, and salinity-alkalinity stresses in tomato, cucumber, rice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 124-20-9
Appearance <23°C Solid,>25°C Liquid
Molecular Weight 145.25
Formula C₇H₁₉N₃
Color Colorless to light yellow
SMILES

NCCCCNCCCN
Structure Classification
- Others
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (20)

All IFIn Vivo Efficacy Study

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Journal Impact Factor

Cell

Tumor-produced ammonia is metabolized by regulatory T cells to further impede anti-tumor immunity. [Abstract]

2026 Jan 22;189(2):418-434.e24. PMID: 41448182
Cell Metab

Stress triggers irritable bowel syndrome with diarrhea through a spermidine-mediated decline in type I interferon. [Abstract]

2025 Jan 7;37(1):87-103.e10. PMID: 39366386

Spermidine purchased from MedChemExpress. Usage Cited in: Cell Metab. 2025 Jan 7;37(1):87-103.e10. [Abstract]

Spermidine (40 mg/kg; i.p.; every 2 days 3 times). WT (left, n = 3) or GF (right, n = 5) mice are i.p. injected with Spermidine (40 mg/kg) every 2 days 3 times, and the AWR scores are determined on day 8.
Nat Commun

Metabolic support protects mucosa from ferroptosis in radiation-induced mucositis. [Abstract]

2025 Dec 8. PMID: 41361241

Spermidine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 8. [Abstract]

Spermidine (10 mg/kg; i.p.; starting 7 days prior to irradiation and continuing until 9 days post-irradiation). Immunofluorescence staining confirms significantly enhanced epithelial proliferation in the Spermidine-treated group by day 9 post-radiation. Quantification of PCNA+ basal cells before irradiation and for 9 days post-irradiation (n = 6 mice). Five random images per mouse were analyzed. Scale bar, 100 μm.
Nat Commun

USP17L promotes the 2-cell-like program through deubiquitination of H2AK119ub1 and ZSCAN4. [Abstract]

2025 Aug 1;16(1):7071. PMID: 40750602
J Control Release

Targeting polyamine metabolism induces oxidative/carbonyl stress to reinvigorate antitumor immunity in prostate cancer. [Abstract]

2025 Sep 30;388(Pt 1):114283. PMID: 41038348
Cell Rep Med

Ribosome dysregulation and intervention in age-related infertility. [Abstract]

2025 Oct 27:102424. PMID: 41151579
Clin Cancer Res

Spermidine Secreted by Apoptotic Cells Enhances Chemotherapy Resistance by Modulating β-Catenin Activity in Osteosarcoma. [Abstract]

2025 Nov 20. PMID: 41263700
EMBO J

Maternal mRNA deadenylation and allocation via Rbm14 condensates facilitate vertebrate blastula development. [Abstract]

2023 Feb 1;42(3):e111364. PMID: 36477743
PLoS Biol

A high-throughput behavioral screening platform for measuring chemotaxis by C. elegans. [Abstract]

2024 Jun 27;22(6):e3002672. PMID: 38935621
Aging Cell

2026 Jan 20;25(2):e70352.
Aquaculture

2025 Oct 15.
J Biol Chem

Different charged biopolymers induce α-synuclein to form fibrils with distinct structures. [Abstract]

2024 Nov;300(11):107862. PMID: 39374778
PLoS Genet

A C. elegans model of copper deficiency: Dietary interventions rescue CTR1/CHCA-1 copper transporter mutant phenotype. [Abstract]

2026 Jan 23;22(1):e1012013. PMID: 41575937
Vascul Pharmacol

Spermidine ameliorates BK channel dysfunction in diabetic coronary smooth muscle cells via PKM2/STAT3/FBXO32 axis. [Abstract]

2026 Jun 16:164:107672. PMID: 42303097
Sleep Med

Cerebellar GluN2C protects against sleep deprivation-induced cognitive impairment and motor incoordination. [Abstract]

2025 Nov 8:137:108648. PMID: 41223665
Exp Eye Res

Spermidine mitigates blue-light-induced corneal injury by alleviating oxidative stress and restoring autophagy impairment. [Abstract]

2025 Mar 24:110360. PMID: 40139641
Virus Res

PEDV infection affects the expression of polyamine-related genes inhibiting viral proliferation. [Abstract]

2022 Apr 15:312:198708. PMID: 35151773
J Toxicol Sci

Spermidine alleviates sepsis-induced acute kidney injury by suppressing apoptosis and inflammation through modulation of TLR4-mediated signaling. [Abstract]

2025;50(10):555-567. PMID: 41034042
Res Sq

2025 Jul 13.
bioRxiv

An efficient behavioral screening platform classifies natural products and other chemical cues according to their chemosensory valence in C. elegans. [Abstract]

2024 Apr 3:2023.06.02.542933. PMID: 37333363

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (688.47 mM; Need ultrasonic)

DMSO : 16.67 mg/mL (114.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.8847 mL

34.4234 mL

68.8468 mL

5 mM

1.3769 mL

6.8847 mL

13.7694 mL

10 mM

0.6885 mL

3.4423 mL

6.8847 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.67 mg/mL (11.50 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 1.67 mg/mL (11.50 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.67 mg/mL (11.50 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 50 mg/mL (344.23 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
SDS (477 KB)
- English - EN (477 KB)
- Français - FR (477 KB)
- Deutsch - DE (477 KB)
- Norwegian - NO (477 KB)
- Español - ES (477 KB)
- Swedish - SV (477 KB)
- Italian - IT (477 KB)
- Korean - KR (477 KB)
- Portuguese - PT (477 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhang L, et al. The Alleviation of Heat Damage to Photosystem II and Enzymatic Antioxidants by Exogenous Spermidine in Tall Fescue. Front Plant Sci. 2017 Oct 12;8:1747. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	6.8847 mL	34.4234 mL	68.8468 mL	172.1170 mL
	5 mM	1.3769 mL	6.8847 mL	13.7694 mL	34.4234 mL
	10 mM	0.6885 mL	3.4423 mL	6.8847 mL	17.2117 mL
	15 mM	0.4590 mL	2.2949 mL	4.5898 mL	11.4745 mL
	20 mM	0.3442 mL	1.7212 mL	3.4423 mL	8.6059 mL
	25 mM	0.2754 mL	1.3769 mL	2.7539 mL	6.8847 mL
	30 mM	0.2295 mL	1.1474 mL	2.2949 mL	5.7372 mL
	40 mM	0.1721 mL	0.8606 mL	1.7212 mL	4.3029 mL
	50 mM	0.1377 mL	0.6885 mL	1.3769 mL	3.4423 mL
	60 mM	0.1147 mL	0.5737 mL	1.1474 mL	2.8686 mL
	80 mM	0.0861 mL	0.4303 mL	0.8606 mL	2.1515 mL
	100 mM	0.0688 mL	0.3442 mL	0.6885 mL	1.7212 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Spermidine

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Publications Citing Use of MedChemExpress (MCE) Spermidine

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (20)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Spermidine Related Classifications

HY-B1776 Related Classifications