FAZ-3532

Name: FAZ-3532
Brand: MedChemExpress
SKU: HY-162288
Rating: 5.0 (4 reviews)

Cat. No.: HY-162288 Purity: 98.51%: Data Sheet
COA

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Handling Instructions
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FAZ-3532 is a G3BP inhibitor (K_d = 0.54 μM) which binds to the NTF2L nsP3 binding pocket in G3BP1. FAZ-3532 disrupts the co-condensation of RNA, G3BP1, and caprin 1. FAZ-3532 inhibits G3BP-driven stress granule formation. FAZ-3532 can be studied as a powerful tool to probe the biology of stress granules and a promising interventions designed to modulate stress granule formation.

For research use only. We do not sell to patients.

CAS No. : 3036029-72-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

4 Publications Citing Use of MCE FAZ-3532

Cell Imaging/Staining

In Vivo Efficacy Study

Histological Imaging/Staining

: FAZ-3532 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 1;16(1):5610. [Abstract]; Representative immunofluorescence imaging of stress granules (SG) assembly (G3BP1) in BMDMs pre-treated with FAZ-3532 (G3Ia, 20-100 μM) for 20 min followed by HDM stimulation for 30 min.

: FAZ-3532 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 1;16(1):5610. [Abstract]; Mice were administered OVA and treated with PBS, NaAsO₂ (0.015 mg/kg), or FAZ-3532 (G3Ia, 0.05 mg/kg; i.n.; once daily for 7 days). The number of sneezes and scratches in each mouse was counted 15 min after the last i.n. challenge (n = 5, biological replicates). (Dunnett's multiple comparisons test).

: FAZ-3532 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 1;16(1):5610. [Abstract]; Mice were administered OVA and treated with PBS, NaAsO₂ (0.015 mg/kg), or FAZ-3532 (G3Ia, 0.05 mg/kg; i.n.; once daily for 7 days). H&E staining of the nasal mucosa and lung tissues from AR mice. c Flow cytometry of eosinophils, neutrophils and T cells in the nasal mucosa of AR mice (n = 3, biological replicates).

: FAZ-3532 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 1;16(1):5610. [Abstract]; G3BP1 and LRP1 protein levels in PMs pre-treated with FAZ-3532 (G3Ia, 50-100 μM) for 20 min followed by HDM stimulation for 1 h were examined by Western blot.

: FAZ-3532 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Apr 1:1-23. [Abstract]; FaDu cells were cultured in a 96-well hanging drop plate for 72 h to form spheroids. Subsequently, the spheroids were treated with 50 μM FAZ-3532 or vehicle control and further cultured for an additional 3 days. The viable (green) and dead (red) cells were assessed using the Live/DEAD™ Viability/Cytotoxicity Kit for Mammalian Cells. Representative results are shown. Scale bar: 100 μm.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

FAZ-3532 (Compound G3Ia) (10-50 μM) potently displaces the FGDP peptide from the NTF2L domain and exerts tight binding with NTF2L domain^[1].
FAZ-3532 (10 nM-100 μM) disrupts the co-condensation of molecules in the mixture of 1.5 μM of G3BP1, 1.5 μM caprin 1, and 20 ng/μM RNA, where it effectively disrupts the binding of G3BP1 with caprin 1 and reduces condensation of G3BP1 with RNA^[1].
FAZ-3532 (5-50 μM, 20 min) robustly inhibits stress granule formation in a dose-dependent manner in U2OS cells stably expressing G3BP1-GFP with NaAsO₂ (500 μM)-induced stress granule formulation^[1].
FAZ-3532 (50 μM, 2 min) spontaneously reduces stress granule area by ~74% in U2OS cells stably expressing G3BP1-GFP with sodium arsenite (250 μM)-induced stress granule formulation^[1].
FAZ-3532 (50 μM, 60 min) blocks both spontaneous and arsenite-induced stress granule formation in iPSC-derived cortical neurons exposed simultaneously to NaAsO₂ (500 μM)^[1].
FAZ-3532 (50 μM, 30 min) dissolves 83% of the granules in GFP-VCP A232E transfected U2OS cells ^[1].
FAZ-3532 (50 μM, 30 min) reduces the area of G3BP1 found in puncta of FUS R495X transfected U2OS cells compared with vehicle-treated cells, suggesting the inhibiton of G3BP1 condensation does not prevent the inclusion of mutant FUS protein into cytoplasmic puncta^[1].
FAZ-3532 (50 μM, 30 min) Increases ANXA5-FITC-positive apoptotic cells when combined with Cisplatin (HY-17394)^[2].
FAZ-3532 (50 μM, 72 h) reduces tumorsphere size and increases global cell death^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[1]

Cell Line:	U2OS cells stably expressing G3BP1-GFP with NaAsO_{2 (250 μM)-induced stress granule formulation}
Concentration:	50 μM
Incubation Time:	5 min
Result:	Reduced eIF3η, PABPC1, and FXR1 puncta. Indicated disassembly of stress granules beyond simple extraction of G3BP1.

Molecular Weight

713.88

Formula

C₃₈H₅₆FN₅O₇

CAS No.

3036029-72-5

Appearance

Solid

Color

White to off-white

SMILES

C[C@H]([C@H](NC([C@H](C(C)(C)O)NC([C@H](CC(C)(C)C)F)=O)=O)C(N[C@H](C)C(N[C@@H](CCOC)C(N(C)CC1=CC=CC=C1)=O)=O)=O)C2=CC=CC=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 116.67 mg/mL (163.43 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4008 mL	7.0040 mL	14.0080 mL
5 mM	0.2802 mL	1.4008 mL	2.8016 mL
10 mM	0.1401 mL	0.7004 mL	1.4008 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.66%

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Data Sheet (274 KB) SDS (252 KB)

COA (248 KB) LCMS (103 KB)

Handling Instructions (2659 KB)

References

[1]. Brian D Freibaum, et al. Identification of small molecule inhibitors of G3BP-driven stress granule formation. J Cell Biol. 2024 Mar 4;223(3):e202308083. [Content Brief]

[2]. Kim, H. J., et al., (2025). TBK1 is a signaling hub in coordinating stress-adaptive mechanisms in head and neck cancer progression. Autophagy, 1–23. Advance online publication. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4008 mL	7.0040 mL	14.0080 mL	35.0199 mL
	5 mM	0.2802 mL	1.4008 mL	2.8016 mL	7.0040 mL
	10 mM	0.1401 mL	0.7004 mL	1.4008 mL	3.5020 mL
	15 mM	0.0934 mL	0.4669 mL	0.9339 mL	2.3347 mL
	20 mM	0.0700 mL	0.3502 mL	0.7004 mL	1.7510 mL
	25 mM	0.0560 mL	0.2802 mL	0.5603 mL	1.4008 mL
	30 mM	0.0467 mL	0.2335 mL	0.4669 mL	1.1673 mL
	40 mM	0.0350 mL	0.1751 mL	0.3502 mL	0.8755 mL
	50 mM	0.0280 mL	0.1401 mL	0.2802 mL	0.7004 mL
	60 mM	0.0233 mL	0.1167 mL	0.2335 mL	0.5837 mL
	80 mM	0.0175 mL	0.0875 mL	0.1751 mL	0.4377 mL
	100 mM	0.0140 mL	0.0700 mL	0.1401 mL	0.3502 mL

FAZ-3532 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FAZ-35323036029-72-5FAZ3532FAZ 3532DNA/RNA SynthesisG3BP inhibitorNTF2Lcaprin 1RNAco-condensationInhibitorinhibitorinhibit

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FAZ-3532

Customer Review

FAZ-3532 Related Antibodies

4 Publications Citing Use of MCE FAZ-3532

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•Related Screening Libraries:

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View All DNA/RNA Synthesis Isoform Specific Products:

Biological Activity

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References

Customer Review

FAZ-3532 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

FAZ-3532 Related Classifications

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