FAZ-3532
Based on 9 publication(s) in Google Scholar
FAZ-3532 is a G3BP inhibitor (Kd = 0.54 μM) which binds to the NTF2L nsP3 binding pocket in G3BP1. FAZ-3532 disrupts the co-condensation of RNA, G3BP1, and caprin 1. FAZ-3532 inhibits G3BP-driven stress granule formation. FAZ-3532 can be studied as a powerful tool to probe the biology of stress granules and a promising interventions designed to modulate stress granule formation.
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- Pureté: 98.51%
- CAS No.: 3036029-72-5
- Formule: C38H56FN5O7
- Masse moléculaire:713.88
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) FAZ-3532
More- Nat Cell Biol. 2026 Jun;28(6):1204-1218. [Abstract]
- Autophagy. 2025 Aug;21(8):1744-1766. [Abstract]
- Nat Commun. 2025 Jul 1;16(1):5610. [Abstract]
- EMBO J. 2024 Dec;43(24):6410-6443. [Abstract]
- Free Radic Biol Med. 2026 Aug 1:251:440-453. [Abstract]
- Cell Oncol (Dordr). 2026 Apr 20;49(3):74. [Abstract]
- Toxicol Res. 2026 May 24.
- bioRxiv. 2026 May 13:2026.05.11.724403. [Abstract]
- bioRxiv. 2025 Aug 30:2025.08.27.672666. [Abstract]
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Activité biologique
FAZ-3532 (Compound G3Ia) (10-50 μM) potently displaces the FGDP peptide from the NTF2L domain and exerts tight binding with NTF2L domain[1].
FAZ-3532 (10 nM-100 μM) disrupts the co-condensation of molecules in the mixture of 1.5 μM of G3BP1, 1.5 μM caprin 1, and 20 ng/μM RNA, where it effectively disrupts the binding of G3BP1 with caprin 1 and reduces condensation of G3BP1 with RNA[1].
FAZ-3532 (5-50 μM, 20 min) robustly inhibits stress granule formation in a dose-dependent manner in U2OS cells stably expressing G3BP1-GFP with NaAsO2 (500 μM)-induced stress granule formulation[1].
FAZ-3532 (50 μM, 2 min) spontaneously reduces stress granule area by ~74% in U2OS cells stably expressing G3BP1-GFP with sodium arsenite (250 μM)-induced stress granule formulation[1].
FAZ-3532 (50 μM, 60 min) blocks both spontaneous and arsenite-induced stress granule formation in iPSC-derived cortical neurons exposed simultaneously to NaAsO2 (500 μM)[1].
FAZ-3532 (50 μM, 30 min) dissolves 83% of the granules in GFP-VCP A232E transfected U2OS cells [1].
FAZ-3532 (50 μM, 30 min) reduces the area of G3BP1 found in puncta of FUS R495X transfected U2OS cells compared with vehicle-treated cells, suggesting the inhibiton of G3BP1 condensation does not prevent the inclusion of mutant FUS protein into cytoplasmic puncta[1].
FAZ-3532 (50 μM, 30 min) Increases ANXA5-FITC-positive apoptotic cells when combined with Cisplatin (HY-17394)[2].
FAZ-3532 (50 μM, 72 h) reduces tumorsphere size and increases global cell death[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:U2OS cells stably expressing G3BP1-GFP with NaAsO2 (250 μM)-induced stress granule formulation
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Concentration:50 μM
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Incubation Time:5 min
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Result:Reduced eIF3η, PABPC1, and FXR1 puncta.
Indicated disassembly of stress granules beyond simple extraction of G3BP1.
Chemical Information
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CAS No. 3036029-72-5
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Appearance Solid
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Masse moléculaire 713.88
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Formule C38H56FN5O7
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Color White to off-white
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SMILES
C[C@H]([C@H](NC([C@H](C(C)(C)O)NC([C@H](CC(C)(C)C)F)=O)=O)C(N[C@H](C)C(N[C@@H](CCOC)C(N(C)CC1=CC=CC=C1)=O)=O)=O)C2=CC=CC=C2
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (9)
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Journal Impact Factor
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Most Recent
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Nat Cell Biol
2026 Jun;28(6):1204-1218. PMID: 42174134 -
Autophagy
TBK1 is a signaling hub in coordinating stress-adaptive mechanisms in head and neck cancer progression. [Abstract]2025 Aug;21(8):1744-1766. PMID: 40114316
FAZ-3532 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Aug;21(8):1744-1766. [Abstract]
FaDu cells were cultured in a 96-well hanging drop plate for 72 h to form spheroids. Subsequently, the spheroids were treated with 50 μM FAZ-3532 or vehicle control and further cultured for an additional 3 days. The viable (green) and dead (red) cells were assessed using the Live/DEAD™ Viability/Cytotoxicity Kit for Mammalian Cells. Representative results are shown. Scale bar: 100 μm.
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Nat Commun
Stress granule assembly impairs macrophage efferocytosis to aggravate allergic rhinitis in mice. [Abstract]2025 Jul 1;16(1):5610. PMID: 40595582
FAZ-3532 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 1;16(1):5610. [Abstract]
Representative immunofluorescence imaging of stress granules (SG) assembly (G3BP1) in BMDMs pre-treated with FAZ-3532 (G3Ia, 20-100 μM) for 20 min followed by HDM stimulation for 30 min.
FAZ-3532 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 1;16(1):5610. [Abstract]
Mice were administered OVA and treated with PBS, NaAsO2 (0.015 mg/kg), or FAZ-3532 (G3Ia, 0.05 mg/kg; i.n.; once daily for 7 days). The number of sneezes and scratches in each mouse was counted 15 min after the last i.n. challenge (n = 5, biological replicates). (Dunnett's multiple comparisons test).
FAZ-3532 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 1;16(1):5610. [Abstract]
Mice were administered OVA and treated with PBS, NaAsO2 (0.015 mg/kg), or FAZ-3532 (G3Ia, 0.05 mg/kg; i.n.; once daily for 7 days). H&E staining of the nasal mucosa and lung tissues from AR mice. c Flow cytometry of eosinophils, neutrophils and T cells in the nasal mucosa of AR mice (n = 3, biological replicates).
FAZ-3532 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 1;16(1):5610. [Abstract]
G3BP1 and LRP1 protein levels in PMs pre-treated with FAZ-3532 (G3Ia, 50-100 μM) for 20 min followed by HDM stimulation for 1 h were examined by Western blot.
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EMBO J
2024 Dec;43(24):6410-6443. PMID: 39533058 -
Free Radic Biol Med
Advanced oxidation protein products exacerbate osteoarthritis progression by disrupting stress granules assembly via endoplasmic reticulum stress. [Abstract]2026 Aug 1:251:440-453. PMID: 42009145 -
Cell Oncol (Dordr)
Stiffness-related stress granules promote the metastasis of early-stage oral squamous cell carcinoma via anoikis resistance. [Abstract]2026 Apr 20;49(3):74. PMID: 42008177 -
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bioRxiv
Naked antisense oligonucleotides remain endolysosomally sequestered despite induced membrane damage. [Abstract]2026 May 13:2026.05.11.724403. PMID: 42182263 -
bioRxiv
Processing bodies promote lysosomal quality control and cell survival during recovery from lysosomal damage. [Abstract]2025 Aug 30:2025.08.27.672666. PMID: 40909692
Solvant et solubilité
DMSO : 116.67 mg/mL (163.43 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Brian D Freibaum, et al. Identification of small molecule inhibitors of G3BP-driven stress granule formation. J Cell Biol. 2024 Mar 4;223(3):e202308083. [Content Brief]
[2]. Kim, H. J., et al., (2025). TBK1 is a signaling hub in coordinating stress-adaptive mechanisms in head and neck cancer progression. Autophagy, 1–23. Advance online publication. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4008 mL | 7.0040 mL | 14.0080 mL | 35.0199 mL |
| 5 mM | 0.2802 mL | 1.4008 mL | 2.8016 mL | 7.0040 mL | |
| 10 mM | 0.1401 mL | 0.7004 mL | 1.4008 mL | 3.5020 mL | |
| 15 mM | 0.0934 mL | 0.4669 mL | 0.9339 mL | 2.3347 mL | |
| 20 mM | 0.0700 mL | 0.3502 mL | 0.7004 mL | 1.7510 mL | |
| 25 mM | 0.0560 mL | 0.2802 mL | 0.5603 mL | 1.4008 mL | |
| 30 mM | 0.0467 mL | 0.2335 mL | 0.4669 mL | 1.1673 mL | |
| 40 mM | 0.0350 mL | 0.1751 mL | 0.3502 mL | 0.8755 mL | |
| 50 mM | 0.0280 mL | 0.1401 mL | 0.2802 mL | 0.7004 mL | |
| 60 mM | 0.0233 mL | 0.1167 mL | 0.2335 mL | 0.5837 mL | |
| 80 mM | 0.0175 mL | 0.0875 mL | 0.1751 mL | 0.4377 mL | |
| 100 mM | 0.0140 mL | 0.0700 mL | 0.1401 mL | 0.3502 mL |
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