Cat. No.: HY-N4119

Neoeriocitrin

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Based on 1 Customer Validation

Neoeriocitrin is a Naringin (HY-N0153) analogue found in Drynaria Rhizome. Neoeriocitrin induces cells proliferation, differentiation, up-regulates type I collagen, osteocalcin, and key osteogenic markers, and increases ALP activity. Neoeriocitrin increases expression of Runx2, COL I, OCN and Beclin1. Neoeriocitrin inhibits phosphorylation of P38 mitogen-activated protein kinase, reduces acetylcholinesterase (AChE) activity, and increases choline acetyltransferase (ChAT) activity. Neoeriocitrin reduces apoptosis and induces autophagy. Neoeriocitrin can be used for the researches of osteoporosis and Alzheimer's disease.

For research use only. We do not sell to patients.

Purity: 99.97%
CAS No.: 13241-32-2
Formula: C₂₇H₃₂O₁₅
Molecular Weight:596.53

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Neoeriocitrin Chemical Structure

CAS No.: 13241-32-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

AChE

All Cholinesterase (ChE) Isoforms :

AChE
BChE

All p38 MAPK Isoforms :

In Vitro

Neoeriocitrin (2-20 μg/mL; 72 h) modulates MC3T3-E1 cell proliferation in a dose-dependent manner^[1].
Neoeriocitrin (2-20 μg/mL; 5 days) increases MC3T3-E1 cell ALP activity in a dose-dependent manner^[1].
Neoeriocitrin (2 μg/mL; 4-8 days) upregulates expression of osteogenic marker genes Runx2, COL I, and OCN in MC3T3-E1 cells^[1].
Neoeriocitrin (2 μg/mL; 5 days) partially rescues PD98059 (HY-12028)-induced inhibition of ALP activity in MC3T3-E1 cells^[1].
Neoeriocitrin (2 μg/mL; 4-8 days) partially rescues PD98059-induced downregulation of COL I and OCN mRNA expression in MC3T3-E1 cells^[1].
Neoeriocitrin (2.5-200 μM; 1-14 days) significantly and sustainably promotes the proliferation of human dental pulp stem cells^[2].
Neoeriocitrin (2.5-10 μM; 7-21 days) enhances the osteogenic differentiation of human dental pulp stem cells, as measured by increased osteogenic gene and protein expression, alkaline phosphatase activity, and mineralization^[2].
Neoeriocitrin (100 μM; 1 h) directly binds to Beclin1 in human dental pulp stem cells, as identified by increased thermal stability of Beclin1 in thermal proteome profiling^[2].
Neoeriocitrin (2.5-10 μM; 48 h) induces autophagy in human dental pulp stem cells, as measured by reduced P62 levels, increased LC3-II/I ratio, enhanced autophagosome formation, and increased autophagosome number detected via transmission electron microscopy^[2].
Neoeriocitrin (5 μM) requires Beclin1 as a critical mediator for its induced autophagy and osteogenic differentiation in human dental pulp stem cells, as Beclin1 knockdown attenuates and Beclin1 overexpression amplifies Neoeriocitrin's effects^[2].
Neoeriocitrin (5 μM; 3-12 h) stabilizes Beclin1 protein in human dental pulp stem cells by inhibiting ubiquitin-mediated proteasomal degradation, thereby extending Beclin1 half-life^[2].
Neoeriocitrin (6×10⁴ μM; 2 h pre-incubation, 22 h co-incubation with Aβ25-35) protects Aβ25-35 (HY-P0128)-damaged PC12 cells by reducing apoptosis, upregulating ERβ expression, inhibiting P38 protein phosphorylation, and improving cholinergic system function^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

MC3T3-E1 preosteoblast cells
Concentration:

2, 4, 8, 10, 20 μg/mL
Incubation Time:

72 h
Result:

Increased proliferation rate at 2 and 4 μg/mL.
Decreased proliferation rate at 8-10 μg/mL.

Real Time qPCR^[1]

Cell Line:

MC3T3-E1 preosteoblast cells
Concentration:

2 μg/mL
Incubation Time:

4 days (Runx2 and COL I); 8 days (OCN)
Result:

Increased Runx2, COL I and OCN mRNA expression.

Cell Proliferation Assay^[2]

Cell Line:

human dental pulp stem cells (hDPSCs)
Concentration:

.5, 5, 10,
25, 50, 100, and 200 μM
Incubation Time:

, 3, 5, 7, and 14 days
Result:

Produced a sustained, significant enhancement of hDPSCs proliferation from day 5 onwards at 2.5, 5, and 10 μM, compared to control or higher concentrations.
Stimulated proliferation in the first 3 days at concentrations below 200 μM.

Cell Differentiation Assay^[2]

Cell Line:

human dental pulp stem cells (hDPSCs)
Concentration:

2.5,
5, and 10 μM
Incubation Time:

7 days (ALP staining, gene/protein expression); 21 days (alizarin red staining)
Result:

Most significantly upregulated osteogenesis-related mRNA (Collagen I, ALP, OPN, Runx2) and protein (Collagen I, ALP, OPN, Runx2) expression.
Showed the largest positive area percentage in ALP staining at 7 days with 5 μM.
Showed the highest mineralization in alizarin red staining at 21 days with 5 μM.

Cell Autophagy Assay^[2]

Cell Line:

human dental pulp stem cells (hDPSCs)
Concentration:

2.5,
5, and 10 μM
Incubation Time:

48 h
Result:

Significantly reduced P62 protein levels and increased the LC3-II/I ratio at 2.5 and 5 μM.
Showed a diminished, non-significant effect on P62 at 10 μM.
Increased the number of yellow (RFP⁺GFP⁺) puncta (autophagosomes) and the number of autophagosomes visible via TEM at 5 μM, compared to control.

In Vivo

Neoeriocitrin (P3 hDPSCs cultured with Neoeriocitrin for 5 days and then combined with Bio-Oss bone grafting material at a seeding density of 1 × 10⁷ cells) significantly enhances new bone formation in rat critical-sized calvarial defects, with increased autophagy marker expression (Beclin1, LC3) and reduced P62 in regenerating bone tissue^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 13241-32-2
Appearance Solid
Molecular Weight 596.53
Formula C₂₇H₃₂O₁₅
Color White to off-white
SMILES

O=C1C2=C(O)C=C(O[C@H]3[C@@H]([C@H]([C@H](O)[C@@H](CO)O3)O)O[C@@]4([H])[C@@H]([C@@H]([C@@H](O)[C@H](C)O4)O)O)C=C2O[C@H](C5=CC(O)=C(O)C=C5)C1
Structure Classification
- Flavonoids
- Flavonones
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (167.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6764 mL

8.3818 mL

16.7636 mL

5 mM

0.3353 mL

1.6764 mL

3.3527 mL

10 mM

0.1676 mL

0.8382 mL

1.6764 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Li L, et al. Comparison of neoeriocitrin and naringin on proliferation and osteogenic differentiation in MC3T3-E1. Phytomedicine. 2011;18(11):985-989. [Content Brief]

[2]. Wu Y, et al. Neoeriocitrin Targeting Beclin1 Deubiquitination and Autophagy in Osteogenic Differentiation of Human Dental Pulp Stem Cells. Adv Sci (Weinh). 2025;12(43):e04378. [Content Brief]

[3]. NISHIURA M, et al. Flavonoids in Citrus and related genera Part II. Isolation and identification of isonaringin and neoeriocitrin from Citrus[J]. Agricultural and Biological Chemistry, 1971, 35(11): 1683-1690.

[4]. van der Meer JW, et al. Abstract!. Neth J Med. 2002;60(11):418. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6764 mL	8.3818 mL	16.7636 mL	41.9090 mL
	5 mM	0.3353 mL	1.6764 mL	3.3527 mL	8.3818 mL
	10 mM	0.1676 mL	0.8382 mL	1.6764 mL	4.1909 mL
	15 mM	0.1118 mL	0.5588 mL	1.1176 mL	2.7939 mL
	20 mM	0.0838 mL	0.4191 mL	0.8382 mL	2.0955 mL
	25 mM	0.0671 mL	0.3353 mL	0.6705 mL	1.6764 mL
	30 mM	0.0559 mL	0.2794 mL	0.5588 mL	1.3970 mL
	40 mM	0.0419 mL	0.2095 mL	0.4191 mL	1.0477 mL
	50 mM	0.0335 mL	0.1676 mL	0.3353 mL	0.8382 mL
	60 mM	0.0279 mL	0.1397 mL	0.2794 mL	0.6985 mL
	80 mM	0.0210 mL	0.1048 mL	0.2095 mL	0.5239 mL
	100 mM	0.0168 mL	0.0838 mL	0.1676 mL	0.4191 mL

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Neoeriocitrin

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All Cholinesterase (ChE) Isoforms

All p38 MAPK Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Neoeriocitrin Related Classifications

HY-N4119 Related Classifications