CREBtide
Based on 1 Customer Validation
CREBtide is a CREB (cAMP response element binding protein)-like peptide. CREBtide, a synthetic 13 amino acid peptide, has been reported as a PKA and PKC substrate.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 98.42%
- No. CAS: 149155-45-3
- Fòrmula: C73H129N29O19
- Peso molecular:1716.99
-
Almacenamiento:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Pureza y Documentación
- Referencias
-
Help & FAQs
-
HY-P0248
KemptidePKA Substrate -
HY-P0222
PKI(5-24)PKA Inhibitor -
HY-P1292
PKG inhibitor peptidePKG Inhibitor -
HY-P1191
JIP-1(153-163)c-JNK Inhibitor -
HY-P1561
PKG SubstratePKG Substrate -
HY-P1597
MalantidePKA Substrate -
HY-P1909
Casein Kinase Substrates 3Casein Kinase Substrate -
HY-P3745
H1-7 (histone H1 phosphorylation site), PKA SubstratePKA Substrate -
HY-P3786
H-Arg-Gly-Tyr-Ala-Leu-Gly-OHProtein Kinase Inhibitor -
HY-P3929
PKI (14-24)amidePKA Inhibitor
Actividad biológica
Descripciòn
IC50 & Target
PKA[1]
In Vitro
delta-CREB is a spliced variant of cAMP response element binding protein (CREB). CREBtide (KRREILSRRPSYR), a synthetic peptide based on the phosphorylation sequence in delta-CREB. delta-CREB and CREBtide are tested as substrates of cAMP-dependent protein kinase (cAK). The apparent Km of CREBtide phosphorylation by cAK is 3.9 μM, which is 10-fold lower than that of Kemptide (Km=39 μM), the synthetic peptide substrate most often employed for cAK measurement. The Vmax values are 12.4 mumol/(min.mg) for CREBtide and 9.8 mumol/(min.mg) for Kemptide. The apparent Km of CREBtide phosphorylation by cGMP-dependent protein kinase (cGK) is 2.9 μM and the Vmax value is 3.2 mumol/(min.mg). Both delta-CREB and CREBtide are phosphorylated at a much slower rate by cGK as compared with cAK, implying that the high cAK/cGK specificity exhibits by delta-CREB is retained by the peptide[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
-
No. CAS 149155-45-3
-
Appearance Solid
-
Peso molecular 1716.99
-
Fòrmula C73H129N29O19
-
Color White to off-white
-
Sequence
Lys-Arg-Arg-Glu-Ile-Leu-Ser-Arg-Arg-Pro-Ser-Tyr-Arg
-
Sequence Shortening
KRREILSRRPSYR
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
In Vitro:
H2O : 100 mg/mL (58.24 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (29.12 mM); Clear solution; Need ultrasonic
Pureza y Documentación
-
Ficha de datos (275 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
[1]. Wu J, et al. A microPLC-based approach for determining kinase-substrate specificity. Assay Drug Dev Technol. 2007 Aug;5(4):559-66. [Content Brief]
[2]. Colbran JL, et al. cAMP-dependent protein kinase, but not the cGMP-dependent enzyme, rapidly phosphorylates delta-CREB, and a synthetic delta-CREB peptide. Biochem Cell Biol. 1992 Oct-Nov;70(10-11):1277-82. [Content Brief]
[3]. Harum KH, et al. Cognitive impairment in Coffin-Lowry syndrome correlates with reduced RSK2 activation. Neurology. 2001 Jan 23;56(2):207-14. [Content Brief]
[4]. Peng J, et al. Protein kinase C-α signals P115RhoGEF phosphorylation and RhoA activation in TNF-α-induced mouse brain microvascular endothelial cell barrier dysfunction. J Neuroinflammation. 2011 Apr 8;8:28. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.5824 mL | 2.9121 mL | 5.8241 mL | 14.5604 mL |
| 5 mM | 0.1165 mL | 0.5824 mL | 1.1648 mL | 2.9121 mL | |
| 10 mM | 0.0582 mL | 0.2912 mL | 0.5824 mL | 1.4560 mL | |
| 15 mM | 0.0388 mL | 0.1941 mL | 0.3883 mL | 0.9707 mL | |
| 20 mM | 0.0291 mL | 0.1456 mL | 0.2912 mL | 0.7280 mL | |
| 25 mM | 0.0233 mL | 0.1165 mL | 0.2330 mL | 0.5824 mL | |
| 30 mM | 0.0194 mL | 0.0971 mL | 0.1941 mL | 0.4853 mL | |
| 40 mM | 0.0146 mL | 0.0728 mL | 0.1456 mL | 0.3640 mL | |
| 50 mM | 0.0116 mL | 0.0582 mL | 0.1165 mL | 0.2912 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
CREBtide Related Classifications
HY-P1595 Related Classifications
Powered by Bioz