Calcifediol
Based on 14 publication(s) in Google Scholar
Calcifediol (25-hydroxy Vitamin D3) is an effective VDR ligand and VD supplement. Calcifediol is a prohormone of the vitamin D endocrine system (VDES) and is hydroxylated in the liver to produce the active form, calcitriol. Calcifediol can rapidly increase serum VD levels.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.94%
- No. CAS: 19356-17-3
- Fòrmula: C27H44O2
- Peso molecular:400.64
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Almacenamiento:Powder -20°C, 3 years ; In solvent -80°C, 1 year , -20°C, 6 months
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Calcifediol
More- Nat Chem Biol. 2022 Nov;18(11):1196-1203. [Abstract]
- Proc Natl Acad Sci U S A. 2022 Apr 12;119(15):e2117004119. [Abstract]
- Free Radic Biol Med. 2026 Aug 16:252:664-676. [Abstract]
- Ther Adv Endocrinol Metab. 2020 Oct 20:11:2042018820958295. [Abstract]
- Anal Chim Acta. 2026 Jun 16;1418:345855.
- Int J Mol Sci. 2025 Mar 25;26(7):2985. [Abstract]
- Int Immunopharmacol. 2023 Sep:122:110558. [Abstract]
- Int J Mol Sci. 2017 Dec 19;18(12):2764. [Abstract]
- J Clin Endocrinol Metab. 2026 Jan 5:dgaf708. [Abstract]
- Front Pharmacol. 2020 Mar 31:11:200. [Abstract]
- Sci Rep. 2022 Feb 22;12(1):3014. [Abstract]
- Virol Sin. 2024 Oct;39(5):802-811. [Abstract]
- J Sep Sci. 2025 Apr;48(4):e70130. [Abstract]
- Biochem Biophys Res Commun. 2026 Jul 2:820:153865. [Abstract]
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Ver todos los productos específicos de isoformas Endogenous Metabolite
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Actividad biológica
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Human Endogenous Metabolite |
Calcifediol in either liposomes or ethanolic solution has no effect on the release of the proinflammatory cytokine KC from Pseudomonas-infected murine epithelial cells. Treatment of infected, human bronchial 16-HBE cells with Calcifediol liposomes results in a significant reduction in bacterial survival[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 19356-17-3
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Appearance Solid
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Peso molecular 400.64
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Fòrmula C27H44O2
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Color White to off-white
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SMILES
C=C(CC1)/C(C[C@H]1O)=C\C=C2[C@@]3([H])[C@@](CCC\2)(C)[C@@H]([C@H](C)CCCC(C)(C)O)CC3
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Synonyms
25-Hydroxy Vitamin D3
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years In solvent -80°C 1 year -20°C 6 months
Publications (14)
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Journal Impact Factor
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Most Recent
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Nat Chem Biol
2022 Nov;18(11):1196-1203. PMID: 35982227 -
Proc Natl Acad Sci U S A
2022 Apr 12;119(15):e2117004119. PMID: 35394864 -
Free Radic Biol Med
Vitamin D receptor/sirtuin 3 pathway mediates the cardioprotective effects of aerobic exercise in diabetic mice. [Abstract]2026 Aug 16:252:664-676. PMID: 42061479 -
Ther Adv Endocrinol Metab
The protective effect of iodide intake adjustment and 1,25(OH)2D3 supplementation in rat offspring following excess iodide intake. [Abstract]2020 Oct 20:11:2042018820958295. PMID: 35154633 -
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Int J Mol Sci
DKS26 Alleviates Ischemia-Reperfusion Injury-Induced Acute Kidney Injury by Stabilizing Vitamin D Receptors to Inhibit the Inflammatory Pathway of NF-κB P65. [Abstract]2025 Mar 25;26(7):2985. PMID: 40243616
Calcifediol purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 25;26(7):2985. [Abstract]
Western blot detection of p-P65Ser311, P65, TNFα, MCP-1, KIM1, and NGAL expression in cells after administration of the VDR agonist Calcifediol and/or DKS26-treated mRETCs; n = 3. Calcifediol: 300 nM, DKS26: 100 μg/mL.
Calcifediol purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 25;26(7):2985. [Abstract]
Effects of Calcifediol or DKS26 on the nuclear translocation of p-P65Ser311 (red) observed by laser confocal microscopy.
Calcifediol purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 25;26(7):2985. [Abstract]
Expression of p-IKKβ, IKKβ, p-IKBα, and IKBα after treatment with the VDR agonist calcifediol or DKS26 in mRTECs; n = 3. Calcifediol: 300 nM, DKS26: 100 μg/mL.
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Int Immunopharmacol
Vitamin D attenuates DNCB-induced atopic dermatitis-like skin lesions by inhibiting immune response and restoring skin barrier function. [Abstract]2023 Sep:122:110558. PMID: 37393836 -
Int J Mol Sci
Vitamin D and Its Analogues Decrease Amyloid-β (Aβ) Formation and Increase Aβ-Degradation. [Abstract]2017 Dec 19;18(12):2764. PMID: 29257109
Calcifediol purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2017 Dec 19;18(12):2764. [Abstract]
Effect of vitamin D and analogues on Interleukin-1β level. Interleukin-1β (IL-1β) level was determined by enzyme-linked immunosorbent assay (ELISA) technique (n ≥ 3). IL-1β was analyzed in SH-SY5Y wt cells incubated with 100 nM Calcifediol or analogues compared to cells treated with the solvent control.
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J Clin Endocrinol Metab
Leveraging menstrual effluent (ME) to investigate vitamin D and human decidualization, a potential link with fertility. [Abstract]2026 Jan 5:dgaf708. PMID: 41492807
Calcifediol purchased from MedChemExpress. Usage Cited in: J Clin Endocrinol Metab. 2026 Jan 5:dgaf708. [Abstract]
Effects of cholecalciferol and calcifediol treatment on ME-eSC decidualization. ME-eSCs were pre-treated with vehicle (Veh) vs. cholecalciferol at Veh vs. Calcifediol at 0.1 μM 4 hrs prior to decidualization (stimulated with cAMP+MPA).
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Front Pharmacol
Calcipotriol Inhibits NLRP3 Signal Through YAP1 Activation to Alleviate Cholestatic Liver Injury and Fibrosis. [Abstract]2020 Mar 31:11:200. PMID: 32296329 -
Sci Rep
Quantitative NMR (qNMR) spectroscopy based investigation of the absolute content, stability and isomerization of 25-hydroxyvitamin D2/D3 and 24(R),25-dihydroxyvitamin D2 in solution phase. [Abstract]2022 Feb 22;12(1):3014. PMID: 35194108 -
Virol Sin
Antiviral activity of vitamin D derivatives against severe fever with thrombocytopenia syndrome virus in vitro and in vivo. [Abstract]2024 Oct;39(5):802-811. PMID: 39168248 -
J Sep Sci
Rapid Determination of 25-Hydroxyvitamin D3 and 24,25-Dihydroxyvitamin D3 in Human Urine Based on Polystyrene/Graphene Oxide Packed-Fibers Solid-Phase Extraction Coupled With High-Performance Liquid Chromatography and Atmospheric Pressure Chemical Ionization Tandem Mass Spectrometry. [Abstract]2025 Apr;48(4):e70130. PMID: 40219819 -
Biochem Biophys Res Commun
MASH associated lipotoxicity perturbs the expression of hepatic vitamin D bioactivating gene CYP2R1 in humans and mice. [Abstract]2026 Jul 2:820:153865. PMID: 42061004
Solvente y solubilidad
DMSO : ≥ 100 mg/mL (249.60 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.24 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.19 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Castoldi A, et al. Calcifediol-loaded liposomes for local treatment of pulmonary bacterial infections. Eur J Pharm Biopharm. 2016 Nov 22. [Content Brief]
[2]. Wei Zheng, et al. Vitamin D-induced vitamin D receptor expression induces tamoxifen sensitivity in MCF-7 stem cells via suppression of Wnt/β-catenin signaling. Biosci Rep. 2018 Dec 7;38(6):BSR20180595. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4960 mL | 12.4800 mL | 24.9601 mL | 62.4002 mL |
| 5 mM | 0.4992 mL | 2.4960 mL | 4.9920 mL | 12.4800 mL | |
| 10 mM | 0.2496 mL | 1.2480 mL | 2.4960 mL | 6.2400 mL | |
| 15 mM | 0.1664 mL | 0.8320 mL | 1.6640 mL | 4.1600 mL | |
| 20 mM | 0.1248 mL | 0.6240 mL | 1.2480 mL | 3.1200 mL | |
| 25 mM | 0.0998 mL | 0.4992 mL | 0.9984 mL | 2.4960 mL | |
| 30 mM | 0.0832 mL | 0.4160 mL | 0.8320 mL | 2.0800 mL | |
| 40 mM | 0.0624 mL | 0.3120 mL | 0.6240 mL | 1.5600 mL | |
| 50 mM | 0.0499 mL | 0.2496 mL | 0.4992 mL | 1.2480 mL | |
| 60 mM | 0.0416 mL | 0.2080 mL | 0.4160 mL | 1.0400 mL | |
| 80 mM | 0.0312 mL | 0.1560 mL | 0.3120 mL | 0.7800 mL | |
| 100 mM | 0.0250 mL | 0.1248 mL | 0.2496 mL | 0.6240 mL |
Calcifediol Related Classifications
HY-32351 Related Classifications
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