JW74
Based on 6 publication(s) in Google Scholar
JW74 antagonizes LiCl-induced activation of the canonical Wnt signaling with an IC50 of 420 nM.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.05%
- No. CAS: 863405-60-1
- Fòrmula: C24H20N6O2S
- Peso molecular:456.52
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) JW74
More- Regen Biomater. 2024 Aug 23:11:rbae106. [Abstract]
- J Cell Mol Med. 2020 Apr;24(8):4439-4451. [Abstract]
- Sci Rep. 2026 Feb 2;16(1):6940. [Abstract]
- Sci Rep. 2018 Sep 24;8(1):14268. [Abstract]
- Front Biosci (Landmark Ed). 2025 Jun 24;30(6):39259. [Abstract]
- J Tradit Complement Med. 2024 Mar 7;14(5):568-580. [Abstract]
Actividad biológica
IC50: 420 nM (Wnt)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
0.548 μM
Compound: 3, JW74
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Inhibition of mouse Wnt3A signaling in human HEK293 cells after 1 day by super top flash luciferase reporter gene assay
Inhibition of mouse Wnt3A signaling in human HEK293 cells after 1 day by super top flash luciferase reporter gene assay
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[PMID: 22260203] |
| HEK293 | IC50 |
1.01 μM
Compound: 1, JW74
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Inhibition of TNKS1/2 in HEK293 cells assessed as inhibition of Wnt/beta-casein pathway after 24 hrs by luciferase reporter gene assay
Inhibition of TNKS1/2 in HEK293 cells assessed as inhibition of Wnt/beta-casein pathway after 24 hrs by luciferase reporter gene assay
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[PMID: 23473363] |
| SW480 | EC50 |
0.566 μM
Compound: 3, JW74
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Stabilization of Axin2 in human SW480 cells after 24 hrs by sandwich ELISA
Stabilization of Axin2 in human SW480 cells after 24 hrs by sandwich ELISA
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[PMID: 22260203] |
JW74 shows a reduction of canonical Wnt signaling in the ST-Luc assay with an IC50 of 790 nM[1]. The effect of tankyrase inhibition on cellular viability is tested by performing an MTS assay. The cellular viability of U2OS cells treated for 72 h treatment with 10 μM JW74 is reduced to 80%, relative to DMSO-treated cells. Flow cytometry is also performed to determine the expression marker Ki-67 in U2OS following 48 h treatment with DMSO or 10 uM JW74. Ki-67 expression is reduced from 97.5% in DMSO-treated cells to 86.7% in JW74-treated cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 863405-60-1
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Appearance Solid
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Peso molecular 456.52
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Fòrmula C24H20N6O2S
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Color White to off-white
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SMILES
CC1=CC=C(C2=NOC(CSC3=NN=C(C4=CC=NC=C4)N3C5=CC=C(OC)C=C5)=N2)C=C1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (6)
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Journal Impact Factor
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Most Recent
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Regen Biomater
Self-assembled peptide hydrogel loaded with functional peptide Dentonin accelerates vascularized bone tissue regeneration in critical-size bone defects. [Abstract]2024 Aug 23:11:rbae106. PMID: 39263324 -
J Cell Mol Med
Osthole stimulates bone formation, drives vascularization and retards adipogenesis to alleviate alcohol-induced osteonecrosis of the femoral head. [Abstract]2020 Apr;24(8):4439-4451. PMID: 32135036 -
Sci Rep
PARP-1 couples β-catenin/TCF4 signaling to epithelial-mesenchymal transition in endometriosis. [Abstract]2026 Feb 2;16(1):6940. PMID: 41629483 -
Sci Rep
2018 Sep 24;8(1):14268. PMID: 30250042 -
Front Biosci (Landmark Ed)
Norcholic Acid Promotes M1 Macrophage Polarization in Acute Pancreatitis by Activating the Wnt/β-Catenin Pathway. [Abstract]2025 Jun 24;30(6):39259. PMID: 40613297 -
J Tradit Complement Med
Jintiange capsule ameliorates glucocorticoid-induced osteonecrosis of the femoral head in rats by regulating the activity and differentiation of BMSCs. [Abstract]2024 Mar 7;14(5):568-580. PMID: 39262662
Solvente y solubilidad
DMSO : ≥ 50 mg/mL (109.52 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (6.02 mM); Clear solution
This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
The cell lines U2OS, SaOS-2, and KPD are cultured in RPMI-1640. Two to three thousand cells attached overnight in 96-well plates are treated with culturing medium containing 0.1% DMSO (control) or JW74 (10-0.1 μM). Proliferation rates based on cell confluence are determined by live cell imaging. Cellular viability is also determined by MTS assay. Expression of the proliferation marker Ki-67 is performed by staining cells with PE-mouse anti-human Ki-67 and by analyzing the expression by flow cytometry[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[1]
40 female C.B-Igh-1b/IcrTac-Prkdcscid mice are injected subcutaneously (s.c.) at the right posterior flank with 107 SW480 cells diluted in 100 μL PBS. Injections are initiated when tumor formation is visible in 50 % of the animals (7 days). Mice are randomized and divided into three treatment groups: JW74 150 mg/kg, JW74 300 mg/kg and vehicle control, 1 % Tween 80. Daily intra peritoneal (i.p.) injections (200 μL) with two day injection intermissions after every fifth injection day are performed until the experiment end (29 days). At the termination day, 24 hours after the last injection, blood is collected after cardiac puncture and tumors are dissected and weighed. The compound concentration in tumors and blood are determined using on-line and off-line Solid Phase Extraction-Capillary Liquid Chromatography (SPE-CapLC) instrumentation coupled to a Time of Flight (TOF) mass spectrometer. A Zorbax SB C18 5 μm 150×0.3 mm column is used for separation, and a Knauer K-2600 UV detector is used as a complimentary detector.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (283 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Waaler J, et al. Novel synthetic antagonists of canonical Wnt signaling inhibit colorectal cancer cell growth. Cancer Res. 2011 Jan 1;71(1):197-205. [Content Brief]
[2]. Stratford EW, et al. The tankyrase-specific inhibitor JW74 affects cell cycle progression and induces apoptosis and differentiation in osteosarcoma cell lines. Cancer Med. 2014 Feb;3(1):36-46. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1905 mL | 10.9524 mL | 21.9048 mL | 54.7621 mL |
| 5 mM | 0.4381 mL | 2.1905 mL | 4.3810 mL | 10.9524 mL | |
| 10 mM | 0.2190 mL | 1.0952 mL | 2.1905 mL | 5.4762 mL | |
| 15 mM | 0.1460 mL | 0.7302 mL | 1.4603 mL | 3.6508 mL | |
| 20 mM | 0.1095 mL | 0.5476 mL | 1.0952 mL | 2.7381 mL | |
| 25 mM | 0.0876 mL | 0.4381 mL | 0.8762 mL | 2.1905 mL | |
| 30 mM | 0.0730 mL | 0.3651 mL | 0.7302 mL | 1.8254 mL | |
| 40 mM | 0.0548 mL | 0.2738 mL | 0.5476 mL | 1.3691 mL | |
| 50 mM | 0.0438 mL | 0.2190 mL | 0.4381 mL | 1.0952 mL | |
| 60 mM | 0.0365 mL | 0.1825 mL | 0.3651 mL | 0.9127 mL | |
| 80 mM | 0.0274 mL | 0.1369 mL | 0.2738 mL | 0.6845 mL | |
| 100 mM | 0.0219 mL | 0.1095 mL | 0.2190 mL | 0.5476 mL |
JW74 Related Classifications
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