Referencia número: HY-B0193

Prazosin

18 Cited Publications

Revisión del cliente

Based on 18 publication(s) in Google Scholar

Prazosin is an α-adrenergic receptor antagonist. Prazosin can reduce inflammation, relieve anxiety, alleviate panic, prevent memory decline, and modulate the pain-relieving effects of opioids. Prazosin can be used in the study of hypertension and Alzheimer’s disease.

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Pureza: 99.80%
No. CAS: 19216-56-9
Fòrmula: C₁₉H₂₁N₅O₄
Peso molecular:383.40

Almacenamiento:

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Prazosin Chemical Structure

No. CAS: 19216-56-9

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Actividad biológica
- Descripciòn
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (18)
Solvente y solubilidad
- In Vitro
- In Vivo
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

Ver todos los productos específicos de isoformas Adrenergic Receptor :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	>100 μM Compound: Prazosin	TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT2-expressing HEK293 cells TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT2-expressing HEK293 cells	[PMID: 12110607]
HEK293	IC₅₀	0.88 nM Compound: prazosin	Displacement of [3H]Prazosin from human recombinant alpha1D adrenergic receptor expressed in HEK293 cells after 60 mins Displacement of [3H]Prazosin from human recombinant alpha1D adrenergic receptor expressed in HEK293 cells after 60 mins	[PMID: 23403082]
HEK293	IC₅₀	1.6 μM Compound: prazosin	Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay	[PMID: 23241029]
HEK293	IC₅₀	1.84 μM Compound: Prazosin	TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT1-expressing HEK293 cells TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT1-expressing HEK293 cells	[PMID: 12110607]
HEK293	IC₅₀	12.6 μM Compound: Prazosin	TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT3-expressing HEK293 cells TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT3-expressing HEK293 cells	[PMID: 12110607]
HEK293	IC₅₀	13.6 μM Compound: prazosin	Inhibition of human OCT2-mediated ASP+ uptake expressed in HEK293 cells after 3 mins by fluorescence assay Inhibition of human OCT2-mediated ASP+ uptake expressed in HEK293 cells after 3 mins by fluorescence assay	[PMID: 23241029]
HEK293	IC₅₀	38.4 μM Compound: prazosin	Inhibition of human MATE2K-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay Inhibition of human MATE2K-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay	[PMID: 23241029]
HEK293	IC₅₀	9.9 μM Compound: prazosin	Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy	[PMID: 18788725]
LNCaP	IC₅₀	67.46 μM Compound: 1	Antiproliferative activity was determined by colorimetric MTS assay in LNCaP cells after 72 hr of incubation with compounds (1-100 uM) Antiproliferative activity was determined by colorimetric MTS assay in LNCaP cells after 72 hr of incubation with compounds (1-100 uM)	[PMID: 15633998]
PC-3	IC₅₀	11.72 μM Compound: Prazosin	Antiproliferative activity against human PC-3 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK8 assay Antiproliferative activity against human PC-3 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK8 assay	[PMID: 33160761]
PC-3	IC₅₀	11.72 mM Compound: Prazosin	Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth	[PMID: 34411892]

In Vitro

Prazosin (50 μM, 18 h) can reduce the production of amyloid beta in N2a cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

N2asw
Concentration:

50 μM
Incubation Time:

18 h
Result:

Reduced Aβ levels, induced a significant increase in APP-CTFs, and significantly reduced the production of CTFγ.

In Vivo

Prazosin (1 mg/kg, injected into the abdomen once daily, two weeks) can prevent memory deficits in mice by altering the release of inflammatory mediators and astrocytes, alleviating symptoms of Alzheimer's disease^[2].
Prazosin (0.1-2 mg/kg, 3-6 mM, administered systemically or locally, in a single dose) can promote fear extinction in mice^[3].
Prazosin (0.01-1 mg/kg, subcutaneously, in a single dose) can enhance the analgesic effects of morphine in mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

APP23 mice^[2]
Dosage:

1 mg/kg; daily; 2 weeks
Administration:

Intraperitoneal injection (i.p.)
Result:

Increased mean freezing time during acclimation, attenuated hippocampal-dependent memory decline, did not affect APP processing or Aβ aggregation in vivo, and increased the number of astrocytes and secretion of anti-inflammatory mediators in APP23 mice.

Animal Model:

C57B1/6N mouse^[3]
Dosage:

0.1-2 mg/kg; 3 and 6 mM; single dose
Administration:

systemic (0.1-2 mg/kg) or local microinjections (3 or 6 mM) into the prelimbic division of medial prefrontal cortex or basolateral amygdala
Result:

Had no effect on fear memory, but indirectly promoted fear extinction by interfering with the initial fear acquisition process.

Animal Model:

C57BL/6J mice^[4]
Dosage:

0.01, 0.1, 0.25, 0.5, 1 mg/kg; single dose
Administration:

Subcutaneous injection (s.c.)
Result:

Had no analgesic effect when used alone, but it enhanced the analgesic effect of morphine and reduced weight loss after morphine withdrawal.

Chemical Information

No. CAS 19216-56-9
Appearance Solid
Peso molecular 383.40
Fòrmula C₁₉H₂₁N₅O₄
Color White to off-white
SMILES

O=C(N1CCN(C2=NC(N)=C3C=C(OC)C(OC)=CC3=N2)CC1)C4=CC=CO4
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Publications (18)

All ELISAWBFlow CytometryCell Proliferation/Viability AssayRT-PCR

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Journal Impact Factor

Nature

The astrocytic ensemble acts as a multiday trace to stabilize memory. [Abstract]

2025 Dec;648(8092):146-156. PMID: 41094146
Cancer Discov

Myofibroblasts Induce Neuroplasticity to Promote Pancreatic Inflammation and Cancer Progression. [Abstract]

2026 Feb 9. PMID: 41661076
Cell Discov

Adenosine-independent regulation of the sleep-wake cycle by astrocyte activity. [Abstract]

2023 Feb 7;9(1):16. PMID: 36746933
Cell Rep Med

The sympathetic nervous system drives hyperinflammatory responses to Clostridioides difficile infection. [Abstract]

2024 Oct 1:101771. PMID: 39368481
J Exp Med

IL-6 translation is a therapeutic target of human cytokine release syndrome. [Abstract]

2023 Nov 6;220(11):e20230577. PMID: 37584653
J Transl Med

CDK1 serves as a therapeutic target of adrenocortical carcinoma via regulating epithelial-mesenchymal transition, G2/M phase transition, and PANoptosis. [Abstract]

2022 Oct 2;20(1):444. PMID: 36184616
J Med Chem

Development of an In Silico Prediction Model for P-glycoprotein Efflux Potential in Brain Capillary Endothelial Cells toward the Prediction of Brain Penetration. [Abstract]

2021 Mar 11;64(5):2725-2738. PMID: 33619967
Redox Rep

Norepinephrine promotes oxidative stress in vascular adventitial fibroblasts via PKC/NFκB-mediated NOX2 upregulation. [Abstract]

2025 Dec;30(1):2494314. PMID: 40269356

Prazosin purchased from MedChemExpress. Usage Cited in: Redox Rep. 2025 Dec;30(1):2494314. [Abstract]

Prazosin (10 μM) abolished NE-induced increases in NOX2 expression in VAFs of Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR).
Mol Metab

Vagal control of the brain-esophagus axis ameliorates stress-induced esophageal motility dysfunction in male mice. [Abstract]

2026 May 26:102384. PMID: 42203107
Drug Des Devel Ther

Dexmedetomidine Prolongs Lidocaine Intravenous Regional Anesthesia in Rats by Blocking the Hyperpolarization-Activated Cation Current. [Abstract]

2024 Apr 9:18:1103-1114. PMID: 38618283
Cells

Impact of Short-Term (+)-JQ1 Exposure on Mouse Aorta: Unanticipated Inhibition of Smooth Muscle Contractility. [Abstract]

2023 May 24;12(11):1461. PMID: 37296583
Am J Pathol

Hyperglycemia induces tear reduction and dry eye in diabetic mice through the norepinephrine-α1AR -mitochondrial impairment axis of lacrimal gland. [Abstract]

2023 Jul;193(7):913-926. PMID: 37088455
iScience

Stimulation of TRPV1+ peripheral somatosensory nerves suppress inflammation via the somato-autonomic reflex. [Abstract]

2025 Jan 17;28(2):111831. PMID: 39967868
Am J Physiol Renal Physiol

Anatomic and functional evidence for renal autonomic innervation in normotensive and hypertensive rats. [Abstract]

2024 Nov 1;327(5):F885-F898. PMID: 39298550
BMC Pulm Med

Opposing responses of the rat pulmonary artery and vein to phenylephrine and other agents in vitro. [Abstract]

2021 Jun 5;21(1):189. PMID: 34090386

Prazosin purchased from MedChemExpress. Usage Cited in: BMC Pulm Med. 2021 Jun 5;21(1):189. [Abstract]

Prazosin (2 mg/kg; i.p.; 60 min) abolished the inhibition of PAVA on TNF-α production in C57BL/6J male mice.
bioRxiv

2024 November 03.
Elife

2024 May 29.
Biomed Pharmacother

Prazosin inhibits the proliferation and survival of acute myeloid leukaemia cells through down-regulating TNS1. [Abstract]

2020 Apr;124:109731. PMID: 31954876

Prazosin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Apr;124:109731. [Abstract]

The expression of TNS1 in U937 and HL60 cells treated with different concentration of Prazosin is detected by Western blot analysis

Prazosin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Apr;124:109731. [Abstract]

Prazosin (5-15 μM) promoted apoptosis in HL60 and U937 cells in a dose-dependent manner under serum deprivation.

Prazosin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Apr;124:109731. [Abstract]

Cell proliferation was measured by the CCK-8 assay in U937 and HL60 cells treated with Prazosin (5-15 μM).

Prazosin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Apr;124:109731. [Abstract]

Prazosin (15 μM; 24 h) down-regulated the expression of all candidate genes except ABCD1 in U937 cells, with TNS1 being the most significantly down-regulated.

Solvente y solubilidad

In Vitro:

DMSO : 41.67 mg/mL (108.69 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6082 mL

13.0412 mL

26.0824 mL

5 mM

0.5216 mL

2.6082 mL

5.2165 mL

10 mM

0.2608 mL

1.3041 mL

2.6082 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (13.04 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (280 KB)
SDS (393 KB)
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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Day HE, et al. Distribution of alpha 1a-, alpha 1b- and alpha 1d-adrenergic receptor mRNA in the rat brain and spinal cord. J Chem Neuroanat. 1997 Jul;13(2):115-39. [Content Brief]

[2]. Loukia Katsouri, et al. Prazosin, an α(1)-adrenoceptor antagonist, prevents memory deterioration in the APP23 transgenic mouse model of Alzheimer's disease. Neurobiol Aging. 2013 Apr;34(4):1105-15. [Content Brief]

[3]. Elizabeth K Lucas, et al. Prazosin during fear conditioning facilitates subsequent extinction in male C57Bl/6N mice. Psychopharmacology (Berl). 2019 Jan;236(1):273-279. [Content Brief]

[4]. Umit Kazim Ozdoğan, et al. Influence of prazosin and clonidine on morphine analgesia, tolerance and withdrawal in mice. Eur J Pharmacol. 2003 Jan 24;460(2-3):127-34. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6082 mL	13.0412 mL	26.0824 mL	65.2061 mL
	5 mM	0.5216 mL	2.6082 mL	5.2165 mL	13.0412 mL
	10 mM	0.2608 mL	1.3041 mL	2.6082 mL	6.5206 mL
	15 mM	0.1739 mL	0.8694 mL	1.7388 mL	4.3471 mL
	20 mM	0.1304 mL	0.6521 mL	1.3041 mL	3.2603 mL
	25 mM	0.1043 mL	0.5216 mL	1.0433 mL	2.6082 mL
	30 mM	0.0869 mL	0.4347 mL	0.8694 mL	2.1735 mL
	40 mM	0.0652 mL	0.3260 mL	0.6521 mL	1.6302 mL
	50 mM	0.0522 mL	0.2608 mL	0.5216 mL	1.3041 mL
	60 mM	0.0435 mL	0.2174 mL	0.4347 mL	1.0868 mL
	80 mM	0.0326 mL	0.1630 mL	0.3260 mL	0.8151 mL
	100 mM	0.0261 mL	0.1304 mL	0.2608 mL	0.6521 mL

Keywords

Prazosin

18 Cited Publications

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Prazosin

Anti-Infection Compound Library

GPCR/G Protein Compound Library

Neuronal Signaling Compound Library

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Neurodegenerative Disease-related Compound Library

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Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Chinese Baijiu Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) Prazosin

Ver todos los productos específicos de isoformas Adrenergic Receptor

Actividad biológica

Chemical Information

Publications (18)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

Prazosin

Anti-Infection Compound Library

GPCR/G Protein Compound Library

Neuronal Signaling Compound Library

FDA-Approved Drug Library

Drug Repurposing Compound Library

Anti-Cardiovascular Disease Compound Library

NMPA-Approved Drug Library

Neurotransmitter Receptor Compound Library

FDA Approved & Pharmacopeial Drug Library

Anti-Alzheimer's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Antidepressant Compound Library

Human Metabolite Library

Off-patent Drug Library

Antihypertensive Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Chinese Baijiu Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) Prazosin

Ver todos los productos específicos de isoformas Adrenergic Receptor

Actividad biológica

Chemical Information

Publications (18)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

Prazosin Related Classifications

HY-B0193 Related Classifications