BD750
Based on 6 publication(s) in Google Scholar
BD750, an effective immunosuppressant and a JAK3/STAT5 inhibitor, inhibits IL-2-induced JAK3/STAT5-dependent T cell proliferation, with IC50 values of 1.5 μM and 1.1 μM in mouse and human T cells, respectively.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.02%
- No. CAS: 892686-59-8
- Fòrmula: C14H13N3OS
- Peso molecular:271.34
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Almacenamiento:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
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Publications Citing Use of MedChemExpress (MCE) BD750
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
Actividad biológica
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JAK3 |
STAT5 |
BD750 inhibits human T cell proliferation stimulated either by anti-CD3/anti-CD28 mAbs or by alloantigen in a dose-dependent manner with IC50 values of 1.1 ± 0.2 μM and 1.3 ± 0.2 μM respectively[1].
BD750 also inhibits ConA, PMA/ionomycine or alloantigen-induced mouse T cell proliferation and PHA or PMA/ionomycine-induced human T cell proliferation[1].
BD750 (5 or 20 μM) inhibits the LPS-induced JAK-STAT5 signaling in DC[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Purified immature DCs.
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Concentration:1, 5 or 20 μM.
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Incubation Time:12 h.
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Result:Had fewer small dendrites and smaller clusters than typical mDCs (5 or 20 μM).
At a higher dose significantly reduced the levels of LPS-stimulated IL-6, IL-12, TNF-α, IL-1β and IL-23 production by DCs.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female C57BL/6 mice (10 wks old, 19-21 g)[2].
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Dosage:20 μM.
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Administration:IV, treated DC on d 7, 11 and 15 post the first PTX injection (dpi 7, 11 and 15).
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Result:Significantly reduced the frequency of Th1 and Th17 cells and increased the percentage of Tregs compared with mice receiving PBS.
Chemical Information
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No. CAS 892686-59-8
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Appearance Solid
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Peso molecular 271.34
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Fòrmula C14H13N3OS
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Color Off-white to light yellow
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SMILES
OC1=C2CCCCC2=NN1C3=NC4=CC=CC=C4S3
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (6)
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Journal Impact Factor
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Most Recent
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Biomed Pharmacother
Astragalus polysaccharide enhances antitumoral effects of chimeric antigen receptor- engineered (CAR) T cells by increasing CD122+CXCR3+PD-1- memory T cells. [Abstract]2024 Sep 6:179:117401. PMID: 39243425 -
Cell Rep
Shear stress-induced Piezo1 activates CD99L2 to facilitate the initiation of blood circulation. [Abstract]2026 Mar 30;45(4):117200. PMID: 41915472 -
OncoImmunology
LFA-1 regulated by IL-2/STAT5 pathway boosts antitumor function of intratumoral CD8+ T cells for improving anti-PD-1 antibody therapy. [Abstract]2023 Dec 14;13(1):2293511. PMID: 38125721 -
Immun Ageing
Melatonin enhances NK cell function in aged mice by increasing T-bet expression via the JAK3-STAT5 signaling pathway. [Abstract]2024 Sep 5;21(1):59. PMID: 39237911 -
FASEB J
P38γ modulates the lipid metabolism in non-alcoholic fatty liver disease by regulating the JAK-STAT signaling pathway. [Abstract]2023 Jan;37(1):e22716. PMID: 36527390
BD750 purchased from MedChemExpress. Usage Cited in: FASEB J. 2023 Jan;37(1):e22716. [Abstract]
BD750 (0, 2.5, 5, 10, 20, 40 μM; 24 h) decreases AML-12 cells vitality in a concentration-dependent manner.
BD750 purchased from MedChemExpress. Usage Cited in: FASEB J. 2023 Jan;37(1):e22716. [Abstract]
BD750 (24 h) successfully inhibits the JAK–STAT signaling pathway in AML-12 cells. The relevant protein expression levels of the JAK–STAT signaling pathway are detected after transfection of NC-siRNA, P38γ-siRNA, and pEGFP-C1, pEGFP-C1-P38γ and co-cultured with BD750 for 24 h in FFA-treated AML-12 cells.
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Solvente y solubilidad
DMSO : 116.67 mg/mL (429.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.67 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (277 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Y Liu, et al. BD750, a benzothiazole derivative, inhibits T cell proliferation by affecting the JAK3/STAT5 signalling pathway. Br J Pharmacol. 2013 Feb;168(3):632-43. [Content Brief]
[2]. Yan Zhou, et al. Tolerogenic dendritic cells induced by BD750 ameliorate proinflammatory T cell responses and experimental autoimmune encephalitis in mice. Mol Med. 2017 Oct;23:204-214. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6854 mL | 18.4271 mL | 36.8541 mL | 92.1353 mL |
| 5 mM | 0.7371 mL | 3.6854 mL | 7.3708 mL | 18.4271 mL | |
| 10 mM | 0.3685 mL | 1.8427 mL | 3.6854 mL | 9.2135 mL | |
| 15 mM | 0.2457 mL | 1.2285 mL | 2.4569 mL | 6.1424 mL | |
| 20 mM | 0.1843 mL | 0.9214 mL | 1.8427 mL | 4.6068 mL | |
| 25 mM | 0.1474 mL | 0.7371 mL | 1.4742 mL | 3.6854 mL | |
| 30 mM | 0.1228 mL | 0.6142 mL | 1.2285 mL | 3.0712 mL | |
| 40 mM | 0.0921 mL | 0.4607 mL | 0.9214 mL | 2.3034 mL | |
| 50 mM | 0.0737 mL | 0.3685 mL | 0.7371 mL | 1.8427 mL | |
| 60 mM | 0.0614 mL | 0.3071 mL | 0.6142 mL | 1.5356 mL | |
| 80 mM | 0.0461 mL | 0.2303 mL | 0.4607 mL | 1.1517 mL | |
| 100 mM | 0.0369 mL | 0.1843 mL | 0.3685 mL | 0.9214 mL |
BD750 Related Classifications
HY-131140 Related Classifications
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