Chlorothymol
Based on 1 Customer Validation
Chlothymol is a potent positive modulator of the GABAA receptor subunit LGC-37, anticonvulsant, and antibacterial agent. Chlothymol inhibits Pentylenetetrazol-induced c-fos expression. Chlothymol inhibits the growth of Methicillin (HY-121544)-resistant Staphylococcus aureus (MRSA) strains, including LAC, with an MIC of 32 μg/mL. Chlorothymol has protective effects against epileptic seizures in various mouse models.
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- Pureza: 99.70%
- No. CAS: 89-68-9
- Fòrmula: C10H13ClO
- Peso molecular:184.67
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Almacenamiento:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Chlorothymol (25 μM for c-fos detection; 60 min for c-fos detection) suppresses Pentylenetetrazol (PTZ)-induced c-fos expression and reduces seizure-like locomotor behavior in 2-day postfertilization (dpf) and 3-dpf zebrafish embryos/larvae[1].
Chlorothymol (150 μM; 15 min) reduces PTZ-induced head-bobbing convulsions in seizure-prone Caenorhabditis elegans unc-49(e407) mutant strain[1].
Chlorothymol inhibits the growth of Methicillin (HY-121544)-resistant Staphylococcus aureus (MRSA) strains including LAC, with a MIC of 32 μg/mL[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 89-68-9
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Appearance Solid
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Peso molecular 184.67
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Fòrmula C10H13ClO
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Color White to off-white
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SMILES
CC(C1=C(O)C=C(C)C(Cl)=C1)C
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Synonyms
Chlorthymol; 6-Chlorothymol
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvente y solubilidad
DMSO : ≥ 100 mg/mL (541.51 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.54 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Alistair Jones, et al. A multiorganism pipeline for antiseizure drug discovery: Identification of chlorothymol as a novel γ-aminobutyric acidergic anticonvulsant. Epilepsia. 2020 Oct;61(10):2106-2118. [Content Brief]
[2]. Kim BC, et al. 4-Chloro-2-Isopropyl-5-Methylphenol Exhibits Antimicrobial and Adjuvant Activity against Methicillin-Resistant Staphylococcus aureus. J Microbiol Biotechnol. 2022 Jun 28;32(6):730-739. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.4151 mL | 27.0753 mL | 54.1506 mL | 135.3766 mL |
| 5 mM | 1.0830 mL | 5.4151 mL | 10.8301 mL | 27.0753 mL | |
| 10 mM | 0.5415 mL | 2.7075 mL | 5.4151 mL | 13.5377 mL | |
| 15 mM | 0.3610 mL | 1.8050 mL | 3.6100 mL | 9.0251 mL | |
| 20 mM | 0.2708 mL | 1.3538 mL | 2.7075 mL | 6.7688 mL | |
| 25 mM | 0.2166 mL | 1.0830 mL | 2.1660 mL | 5.4151 mL | |
| 30 mM | 0.1805 mL | 0.9025 mL | 1.8050 mL | 4.5126 mL | |
| 40 mM | 0.1354 mL | 0.6769 mL | 1.3538 mL | 3.3844 mL | |
| 50 mM | 0.1083 mL | 0.5415 mL | 1.0830 mL | 2.7075 mL | |
| 60 mM | 0.0903 mL | 0.4513 mL | 0.9025 mL | 2.2563 mL | |
| 80 mM | 0.0677 mL | 0.3384 mL | 0.6769 mL | 1.6922 mL | |
| 100 mM | 0.0542 mL | 0.2708 mL | 0.5415 mL | 1.3538 mL |
Chlorothymol Related Classifications
HY-B1606 Related Classifications
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