Garcinone D
Based on 4 publication(s) in Google Scholar
Garcinone D is an activator of the STAT3/Cyclin D1 and Nrf2/HO-1 pathways, and an inhibitor of CDK2/CyclinE1 (IC50 for CDK2/CyclinE1 is 28.23 μM). Garcinone D promotes neural stem cell proliferation by activating STAT3 phosphorylation and Cyclin D1 expression and enhancing the Nrf2/HO-1 signaling pathway. In addition, Garcinone D blocks the tumor cell cycle by inhibiting CDK2/CyclinE1. Garcinone D can promote the proliferation of C17.2 neural stem cells and inhibit prostate and breast cancer.
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- Pureza: 99.30%
- No. CAS: 107390-08-9
- Fòrmula: C24H28O7
- Peso molecular:428.47
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Publications Citing Use of MedChemExpress (MCE) Garcinone D
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Actividad biológica
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p-STAT3 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa S3 | IC50 |
>10 μM
Compound: 13
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Cytotoxicity in human HeLaS3 cells incubated for 72 hrs by MTT assay
Cytotoxicity in human HeLaS3 cells incubated for 72 hrs by MTT assay
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[PMID: 30978023] |
| HepG2 | IC50 |
>10 μM
Compound: 13
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Cytotoxicity in human HepG2 cells incubated for 72 hrs by MTT assay
Cytotoxicity in human HepG2 cells incubated for 72 hrs by MTT assay
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[PMID: 30978023] |
| HT-29 | ED50 |
2.3 μM
Compound: 7
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Cytotoxicity against human HT-29 cells
Cytotoxicity against human HT-29 cells
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[PMID: 19839614] |
| HT-29 | IC50 |
>10 μM
Compound: 13
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Cytotoxicity in human HT-29 cells incubated for 72 hrs by MTT assay
Cytotoxicity in human HT-29 cells incubated for 72 hrs by MTT assay
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[PMID: 30978023] |
| KB | IC50 |
>10 μM
Compound: 13
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Cytotoxicity in human KB cells incubated for 72 hrs by MTT assay
Cytotoxicity in human KB cells incubated for 72 hrs by MTT assay
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[PMID: 30978023] |
| MCF7 | IC50 |
>10 μM
Compound: 13
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Cytotoxicity in human MCF7 cells incubated for 72 hrs by MTT assay
Cytotoxicity in human MCF7 cells incubated for 72 hrs by MTT assay
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[PMID: 30978023] |
Garcinone D (5-400 μM; 24 h) regulates the cell viability of C17.2 neural stem cells. And increases cell viability at 5-10 μM, while significantly reduces cell viability at 20-40 μM[1].
Garcinone D (5 μM; 24 h) promotes the transition of C17.2 neural stem cells in the G1-S phase and arrests the cell cycle in the S phase[1].
Garcinone D (3-10 μM; 6-24 h) increases the levels of p-STAT3, Cyclin D1, Nrf2 and HO-1 proteins in C17.2 neural stem cells in a concentration- and time-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:C17.2 neural stem cells
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Concentration:5 μM, 10 μM, 20 μM, 40 μM
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Incubation Time:24 h
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Result:Significantly increased cell viability at 5 and 10 μM; and significantly decreased cell viability at at 20 and 40 μM.
The percentage of BrdU-positive cells was 1.4-fold higher in the 5 μM group compared with the control, indicating enhanced cell proliferation.
The proportion of cells in the S phase increased from 39.12% to 54.55% after treatment with 5 μM, suggesting promotion of G1-S transition.
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Cell Line:C17.2 neural stem cells
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Concentration:3 μM, 5 μM, 10 μM
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Incubation Time:3 h, 6 h, 12 h, 24 h
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Result:Increased the protein levels of p-STAT3, Cyclin D1, Nrf2, and HO-1 in a dose- and time-dependent manner.
Chemical Information
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No. CAS 107390-08-9
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Appearance Solid
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Peso molecular 428.47
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Fòrmula C24H28O7
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Color Light yellow to yellow
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SMILES
CC(C)(O)CCC1=C(OC)C(O)=CC(OC2=CC(O)=C(C/C=C(C)/C)C(O)=C23)=C1C3=O
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Phytomedicine
Glycosides from Buyang Huanwu Decoction inhibit atherosclerotic inflammation via JAK/STAT signaling pathway. [Abstract]2022 Oct:105:154385. PMID: 35987015 -
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J Immunol
2025 Jun 28:vkaf125. PMID: 40580518 -
Solvente y solubilidad
DMSO : 50 mg/mL (116.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Yang X, et al. Garcinone D, a natural xanthone promotes C17.2 neural stem cell proliferation: Possible involvement of STAT3/Cyclin D1 pathway and Nrf2/HO-1 pathway. Neurosci Lett. 2016 Jul 28;626:6-12. [Content Brief]
[2]. Nauman MC, et al. Inhibition of CDK2/CyclinE1 by xanthones from the mangosteen (Garcinia mangostana): a structure-activity relationship study. Nat Prod Res. 2021 Dec;35(23):5429-5433. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3339 mL | 11.6694 mL | 23.3389 mL | 58.3471 mL |
| 5 mM | 0.4668 mL | 2.3339 mL | 4.6678 mL | 11.6694 mL | |
| 10 mM | 0.2334 mL | 1.1669 mL | 2.3339 mL | 5.8347 mL | |
| 15 mM | 0.1556 mL | 0.7780 mL | 1.5559 mL | 3.8898 mL | |
| 20 mM | 0.1167 mL | 0.5835 mL | 1.1669 mL | 2.9174 mL | |
| 25 mM | 0.0934 mL | 0.4668 mL | 0.9336 mL | 2.3339 mL | |
| 30 mM | 0.0778 mL | 0.3890 mL | 0.7780 mL | 1.9449 mL | |
| 40 mM | 0.0583 mL | 0.2917 mL | 0.5835 mL | 1.4587 mL | |
| 50 mM | 0.0467 mL | 0.2334 mL | 0.4668 mL | 1.1669 mL | |
| 60 mM | 0.0389 mL | 0.1945 mL | 0.3890 mL | 0.9725 mL | |
| 80 mM | 0.0292 mL | 0.1459 mL | 0.2917 mL | 0.7293 mL | |
| 100 mM | 0.0233 mL | 0.1167 mL | 0.2334 mL | 0.5835 mL |
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