SMBA1
Based on 1 Customer Validation
SMBA1 is a potent Bax agonist with a Ki value of 43.3 nM. SMBA1 enhances the Bax expression. SMBA1 shows anti-tumor activity. SMBA1 has the potential for the research of lung cancer.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.5800%
- No. CAS: 906440-37-7
- Fòrmula: C20H13NO3
- Peso molecular:315.32
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
IC50 & Target
[1]|
Bax 43.3 nM (Ki) |
In Vitro
SMBA1 induces conformational changes in Bax by blocking S184 phosphorylation, facilitating Bax insertion into mitochondrial membranes and forming Bax oligomers shows Bax agonist activity[1].
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SMBA1 (0, 0.1, 1, 5, 10 µM; 24 h) enhances the Bax expression in a dose-dependent manner in A549 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549 cells
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Concentration:0, 0.1, 1, 5, 10 µM
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Incubation Time:24 h
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Result:Increased the Bax expression in a dose-dependent manner
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:6-week-old male Nu/Nu mice (A549 lung cancer xenografts)[1]
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Dosage:2, 10, 40, 60 mg/kg
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Administration:I.p.; once daily for 10 days
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Result:Supressed the tumor volume and increased levels of active caspase 3.
Chemical Information
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No. CAS 906440-37-7
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Appearance Solid
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Peso molecular 315.32
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Fòrmula C20H13NO3
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Color Light yellow to yellow
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SMILES
OC1=CC=CC=C1/C=C2C3=C(C4=C\2C=CC=C4)C=CC([N+]([O-])=O)=C3
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (317.14 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.93 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1714 mL | 15.8569 mL | 31.7138 mL | 79.2845 mL |
| 5 mM | 0.6343 mL | 3.1714 mL | 6.3428 mL | 15.8569 mL | |
| 10 mM | 0.3171 mL | 1.5857 mL | 3.1714 mL | 7.9285 mL | |
| 15 mM | 0.2114 mL | 1.0571 mL | 2.1143 mL | 5.2856 mL | |
| 20 mM | 0.1586 mL | 0.7928 mL | 1.5857 mL | 3.9642 mL | |
| 25 mM | 0.1269 mL | 0.6343 mL | 1.2686 mL | 3.1714 mL | |
| 30 mM | 0.1057 mL | 0.5286 mL | 1.0571 mL | 2.6428 mL | |
| 40 mM | 0.0793 mL | 0.3964 mL | 0.7928 mL | 1.9821 mL | |
| 50 mM | 0.0634 mL | 0.3171 mL | 0.6343 mL | 1.5857 mL | |
| 60 mM | 0.0529 mL | 0.2643 mL | 0.5286 mL | 1.3214 mL | |
| 80 mM | 0.0396 mL | 0.1982 mL | 0.3964 mL | 0.9911 mL | |
| 100 mM | 0.0317 mL | 0.1586 mL | 0.3171 mL | 0.7928 mL |
SMBA1 Related Classifications
HY-101996 Related Classifications
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