TRIM28 - tripartite motif containing 28 Gene

Also Known as KAP1; TF1B; RNF96; TIF1B; PPP1R157; TIF1beta

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 10155

About TRIM28

Cytogenetic location: 19q13.43 Genomic coordinates (GRCh38): 19:58,544,064-58,550,715 (from NCBI)

This gene has 15 transcripts (splice variants), 143 orthologues, 80 paralogues and is associated with 1 phenotype. Ubiquitous expression in ovary (RPKM 89.1), testis (RPKM 80.5) and 25 other tissues.

Summary

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

TRIM28 Products (1)

mRNA	Protein	Name
NM_005762.3	NP_005753.1	transcription intermediary factor 1-beta

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables DNA binding	IDA IDA: Inferred from direct assay	9016654	GOA
enables Krueppel-associated box domain binding	IDA IDA: Inferred from direct assay	17512541	GOA
enables SUMO transferase activity	EXP EXP: Inferred from Experiment	17298944	GOA
enables chromatin binding	IDA IDA: Inferred from direct assay	27029610	GOA
enables chromo shadow domain binding	IPI IPI: Inferred from physical interaction	15882967	GOA
enables promoter-specific chromatin binding	IDA IDA: Inferred from direct assay	24623306	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	9016654	GOA
enables transcription corepressor activity	IDA IDA: Inferred from direct assay	8769649	GOA
enables ubiquitin protein ligase activity	IMP IMP: Inferred from mutant phenotype	36354136	GOA
enables ubiquitin protein ligase binding	IDA IDA: Inferred from direct assay	18082607	GOA
enables ubiquitin-protein transferase activity	IDA IDA: Inferred from direct assay	18082607	GOA
enables zinc ion binding	IDA IDA: Inferred from direct assay	10653693	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in DNA repair	IDA IDA: Inferred from direct assay	17178852	GOA
involved in innate immune response	IDA IDA: Inferred from direct assay	18248090	GOA
involved in negative regulation of DNA-templated transcription	IDA IDA: Inferred from direct assay	9016654	GOA
involved in positive regulation of DNA methylation-dependent heterochromatin formation	IMP IMP: Inferred from mutant phenotype	24623306	GOA
involved in positive regulation of DNA repair	IDA IDA: Inferred from direct assay	17178852	GOA
involved in positive regulation of protein import into nucleus	IDA IDA: Inferred from direct assay	23665872	GOA
involved in proteasome-mediated ubiquitin-dependent protein catabolic process	IMP IMP: Inferred from mutant phenotype	36354136	GOA
involved in protein sumoylation	IDA IDA: Inferred from direct assay	18082607	GOA
involved in suppression of viral release by host	IDA IDA: Inferred from direct assay	18248090	GOA

Cellular Component GO Annotation	Evidence	References	Source
located in nucleus	IDA IDA: Inferred from direct assay	8769649	GOA
part of protein-containing complex	IDA IDA: Inferred from direct assay	17512541	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

TRIM28 Protein Structure

zf-RING_5

zf-B_box

PHD

0
200
400
600
835 a.a.

Protein Preferred Names	Protein Names
transcription intermediary factor 1-beta	E3 SUMO-protein ligase TRIM28

TRIM28 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	TRIM28	Q13263	MAGEA6	Homo sapiens	P43360	Pull Down	20864041
Intra	TRIM28	Q13263	ZNF10	Homo sapiens	P21506	Anti Tag CoIP	35271311
Intra	TRIM28	Q13263	ZFP90	Homo sapiens	Q8TF47-3	Anti Tag CoIP	23543754
Intra	TRIM28	Q13263	ZNF84	Homo sapiens	P51523	Anti Tag CoIP	33961781
Intra	TRIM28	Q13263	NCOR1	Homo sapiens	O75376	Chromatography	11013263
Intra	TRIM28	Q13263	NCOR1	Homo sapiens	O75376	IF	11013263
Intra	TRIM28	Q13263	ZNF350	Homo sapiens	Q9GZX5	Anti Tag CoIP	22975076
Intra	TRIM28	Q13263	ZNF649	Homo sapiens	Q9BS31	Anti Tag CoIP	33961781
Intra	TRIM28	Q13263	ZNF649	Homo sapiens	Q9BS31	Anti Tag CoIP	35271311
Intra	TRIM28	Q13263	CHD3	Homo sapiens	Q12873	Y2H Pooling	16169070
Intra	TRIM28	Q13263	CHD3	Homo sapiens	Q12873	Anti Tag CoIP	21642969
Intra	TRIM28	Q13263	MAGEA2	Homo sapiens	P43356	Pull Down	20864041
Intra	TRIM28	Q13263	MAGEA2	Homo sapiens	P43356	TAP	20864041
Intra	TRIM28	Q13263	MAGEA2	Homo sapiens	P43356	Ubiquitinase Assay	20864041
Intra	TRIM28	Q13263	MAGEA2	Homo sapiens	P43356	Anti Tag CoIP	20864041
Intra	TRIM28	Q13263	MAGEA3	Homo sapiens	P43357	Pull Down	20864041
Intra	TRIM28	Q13263	MAGEC2	Homo sapiens	Q9UBF1	Anti Bait CoIP	20864041
Intra	TRIM28	Q13263	MAGEC2	Homo sapiens	Q9UBF1	GMS	20864041
Intra	TRIM28	Q13263	MAGEC2	Homo sapiens	Q9UBF1	Anti Bait CoIP	21277283
Intra	TRIM28	Q13263	MAGEC2	Homo sapiens	Q9UBF1	IF	20864041
Intra	TRIM28	Q13263	MAGEC2	Homo sapiens	Q9UBF1	Pull Down	20864041
Intra	TRIM28	Q13263	MAGEC2	Homo sapiens	Q9UBF1	Ubiquitinase Assay	20864041
Intra	TRIM28	Q13263	MAGEC2	Homo sapiens	Q9UBF1	Anti Tag CoIP	20864041
Intra	TRIM28	Q13263	ZNF136	Homo sapiens	P52737	Anti Tag CoIP	33961781
Intra	TRIM28	Q13263	ZNF136	Homo sapiens	P52737	Anti Tag CoIP	31403225
Cross	TRIM28	Q13263	Cbx1	Mus musculus	P83917	Pull Down	10330177
Intra	TRIM28	Q13263	CBX3	Homo sapiens	Q13185	Pull Down	10330177
Intra	TRIM28	Q13263	CBX3	Homo sapiens	Q13185	Anti Tag CoIP	33961781
Intra	TRIM28	Q13263	CBX3	Homo sapiens	Q13185	Anti Tag CoIP	35271311
Intra	TRIM28	Q13263	CBX5	Homo sapiens	P45973	Pull Down	10330177
Intra	TRIM28	Q13263	CBX5	Homo sapiens	P45973	Anti Tag CoIP	33961781
Intra	TRIM28	Q13263	CBX5	Homo sapiens	P45973	Y2H	20562864
Intra	TRIM28	Q13263	CBX5	Homo sapiens	P45973	Anti Tag CoIP	35271311
Intra	TRIM28	Q13263	ZNF765	Homo sapiens	Q7L2R6	Anti Tag CoIP	33961781

Cross: Cross-species interaction Intra: Intraspecies interaction

TRIM28 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P80730	KAP1 Antibody (YA3523)	WB, IHC-F, IHC-P, ICC/IF	Human, Mouse, Rat
HY-P80730A	KAP1 Antibody (YA3523)(PBS only)	WB, IHC-F, IHC-P, ICC/IF	Human, Mouse, Rat
HY-P810547	Phospho-KAP1 (Ser824) Antibody (YA9816)	WB, IHC-P, IP, Dot Blot, ICC/IF	Human, Mouse, Rat
HY-P85505	KAP1 Antibody (YA5197)	WB, IHC-P, IF-Tissue	Human, Mouse, Rat
HY-P86531	KAP1 Antibody (YA6223)	WB, IHC-P, ICC/IF, IP, ELISA	Human, Mouse, Rat

Related Diseases

Diseases	Alias
Wilms Tumor 1	Nephroblastoma Wilms Tumor WT1 Wilms' Tumor Bilateral Wilms Tumor Wilms Tumor, Type 1 Wilms Tumor, Somatic Adult Nephroblastoma Wt1 Disorder Renal Embryonic Tumor Adult Kidney Wilms Tumor Childhood Kidney Wilms Tumor Nonanaplastic Kidney Wilms Tumor
Wilms Tumor 5	Wilms Tumor WT5 Wilms Tumor Susceptibility-5 Wilms Tumor And Radial Bilateral Aplasia Nephroblastoma Wilms' Tumor Wilms Tumor, Susceptibility To Wtsl Bilateral Radial Aplasia With Wilms Tumor Embryonal Adenosarcoma Embryonal Nephroma Kidney Wilms Tumor Kidney, Adenomyosarcoma, Embryonal Kidney, Carcinosarcoma, Embryonal Kidney, Embryoma Kidney, Embryonal Mixed Tumor Nephroma Renal Adenosarcoma Renal Cancer, Wilms Renal Wilms Tumor Tumor, Wilms Hereditary Susceptibility To Wilms Tumor 5
Hyperoxaluria, Primary, Type I	Primary Hyperoxaluria Type 1 HP1 Glycolic Aciduria Alanine-Glyoxylate Aminotransferase Deficiency Hepatic Agt Deficiency Oxalosis I Primary Hyperoxaluria, Type I Serine:Pyruvate Aminotransferase Deficiency Hyperoxaluria, Primary, Type 1 Peroxisomal Alanine-Glyoxylate Aminotransferase Deficiency Peroxisomal Alanine Glyoxylate Aminotransferase Deficiency Serine Pyruvate Aminotransferase Deficiency Peroxisomal Alanine:Glyoxylate Aminotransferase Deficiency Oxalosis 1 Hyperoxaluria Primary 1 Hyperoxaluria Primary Type I Ph1 Primary Hyperoxaluria Type I Oxalosis Type 1 2-Oxoglutarate Glyoxylate Carboligase Deficiency
Primary Hyperoxaluria	Hyperoxaluria Hyperoxaluria, Primary Oxalosis Primary Oxalosis Congenital Oxaluria D-Glycerate Dehydrogenase Deficiency Glyceric Aciduria Glycolic Aciduria Hepatic Agt Deficiency Oxaluria, Primary Peroxisomal Alanine:Glyoxylate Aminotransferase Deficiency Primary Oxaluria Hyperoxaluria Primary Primary Hyperoxaluria Type 2 Primary Hyperoxaluria, Type I
Hereditary Wilms' Tumor	Hereditary Wilms Tumor Hereditary Wilms Tumour Hereditary Wilms' Tumour Wt1
Colorectal Cancer	Colon Cancer Colorectal Carcinoma Colon Carcinoma Colorectal Cancer, Susceptibility To Carcinoma Of Colon CRC Colorectal Cancer With Chromosomal Instability, Somatic Colon Cancer, Somatic Colon Cancer, Susceptibility To Colonic Neoplasms Colorectal Neoplasms Colorectal Cancer, Somatic Colon Cancer, Advanced, Somatic Colonic Carcinoma Colorectal Carcinomas Colon Cancers Colorectal Cancers Cancer, Colorectal, Somatic Cancer, Colon Cancer, Colorectal, Susceptibility To Colorectal Neoplasm Colonic Neoplasm Malignant Tumor Of Colon
Burkitt Lymphoma	Burkitt'S Lymphoma BL Burkitt Lymphoma, Somatic Burkitt Lymphoma/Leukaemia Burkitt'S Tumor Burkitt'S Tumor Or Lymphoma Malignant Lymphoma, Burkitt'S Type Small Non-Cleaved Cell Lymphoma, Burkitt'S Type Small Non-Cleaved Cell Lymphoma Burkitt Tumor Burkitts Lymphoma Lymphoma, Small Noncleaved-Cell Burkitt Tumour Diffuse Small Noncleaved Malignant Burkitt Lymphoma Malignant Burkitt Lymphoma “Burkitt-Like” Lymphoma Undifferentiated Burkitt Lymphoma Small Noncleaved Cell Burkitt Lymphoma
Transient Neonatal Diabetes Mellitus	Diabetes Mellitus, Transient Neonatal Tndm Chromosome 6-Associated Transient Diabetes Mellitus Dmtn Diabetes Mellitus, 6q24-Related Transient Neonatal Tndm1 Neonatal Diabetes Mellitus, Transient Tndm -[Transient Neonatal Diabetes Mellitus]
Autism Spectrum Disorder	Asd Autism Spectrum Disorders Autistic Continuum Pervasive Developmental Disorder Pervasive Development Disorder Autistic Behavior Autistic Disorder Autistic Autistic Disorder Of Childhood Onset Infantile Autism Childhood Autism Kanner Syndrome Pervasive Developmental Delay Nos Pervasive Developmental Disorder, Not Otherwise Specified
Autism	Autistic Disorder Autism Susceptibility 1 Childhood Autism Autistic Disorder Of Childhood Onset Infantile Autism Kanner'S Syndrome Autistic

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (7)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-169087	MJBIA9836		99.67%	Unkown
HY-RS15033	TRIM28 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-169086	KL-465		/	Unkown
HY-175240	PROTAC BRD4 Degrader-38	Degrader	/	Unkown
HY-181157	T/E PPI-IN-1	Inhibitor	/	Unkown
HY-RS18012	Trim28 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS24485	Trim28 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Rattus norvegicus	TRIM28	RGD	RGD:620289
Macaca mulatta	TRIM28	VGNC	VGNC:79033
Bos taurus	TRIM28	VGNC	VGNC:36324
Felis catus	TRIM28	VGNC	VGNC:82332
Mus musculus	TRIM28	MGD	MGI:109274
Canis familiaris	TRIM28	VGNC	VGNC:47811
Others	TRIM28	NCBI