PRKACA - protein kinase cAMP-activated catalytic subunit alpha Gene

Also Known as CAFD1; PKACA; PPNAD4

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 5566

About PRKACA

Cytogenetic location: 19p13.12 Genomic coordinates (GRCh38): 19:14,091,688-14,117,762 (from NCBI)

This gene has 13 transcripts (splice variants), 1 gene allele, 289 orthologues, 5 paralogues and is associated with 62 phenotypes. Ubiquitous expression in heart (RPKM 62.0), adrenal (RPKM 43.9) and 25 other tissues.

Summary

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and Apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]

PRKACA Products (5)

mRNA	Protein	Name
NM_001304349.2	NP_001291278.1	cAMP-dependent protein kinase catalytic subunit alpha isoform 3
XM_047439070.1	XP_047295026.1	cAMP-dependent protein kinase catalytic subunit alpha isoform X2
NM_207518.3	NP_997401.1	cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha2
XM_017026948.2	XP_016882437.1	cAMP-dependent protein kinase catalytic subunit alpha isoform X1
NM_002730.4	NP_002721.1	cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables cAMP-dependent protein kinase activity	IDA IDA: Inferred from direct assay	11029056	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	11035810	GOA
enables protein kinase A regulatory subunit binding	IPI IPI: Inferred from physical interaction	17911601	GOA
enables protein kinase activity	IDA IDA: Inferred from direct assay	12475942	GOA
enables protein kinase binding	IPI IPI: Inferred from physical interaction	11035810	GOA
enables protein serine kinase activity	IDA IDA: Inferred from direct assay	22797923	GOA
enables protein serine/threonine kinase activity	IDA IDA: Inferred from direct assay	21085490	GOA
enables protein serine/threonine kinase activity	IMP IMP: Inferred from mutant phenotype	26537248	GOA
enables protein serine/threonine/tyrosine kinase activity	IDA IDA: Inferred from direct assay	22797923	GOA
enables ubiquitin protein ligase binding	IDA IDA: Inferred from direct assay	21423175	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway	IDA IDA: Inferred from direct assay	38503280	GOA
involved in cAMP/PKA signal transduction	IDA IDA: Inferred from direct assay	21812984	GOA
involved in cellular response to glucose stimulus	IDA IDA: Inferred from direct assay	19949837	GOA
involved in cellular response to heat	IDA IDA: Inferred from direct assay	21085490	GOA
involved in mRNA processing	IDA IDA: Inferred from direct assay	17594903	GOA
involved in negative regulation of TORC1 signaling	IDA IDA: Inferred from direct assay	31112131	GOA
involved in peptidyl-serine phosphorylation	IDA IDA: Inferred from direct assay	10805756	GOA
involved in peptidyl-serine phosphorylation	IMP IMP: Inferred from mutant phenotype	26537248	GOA
involved in positive regulation of cholesterol biosynthetic process	IDA IDA: Inferred from direct assay	38503280	GOA
involved in protein phosphorylation	IDA IDA: Inferred from direct assay	12475942	GOA
involved in regulation of bicellular tight junction assembly	IDA IDA: Inferred from direct assay	15905176	GOA
involved in regulation of osteoblast differentiation	IDA IDA: Inferred from direct assay	19949837	GOA
involved in regulation of proteasomal protein catabolic process	IDA IDA: Inferred from direct assay	17565987	GOA

Cellular Component GO Annotation	Evidence	References	Source
colocalizes with axoneme	IDA IDA: Inferred from direct assay	12475942	GOA
part of cAMP-dependent protein kinase complex	IDA IDA: Inferred from direct assay	12475942	GOA
located in centrosome	IDA IDA: Inferred from direct assay	21399614	GOA
located in cytoplasm	IDA IDA: Inferred from direct assay	21085490	GOA
located in nuclear speck	IDA IDA: Inferred from direct assay	17594903	GOA
part of nucleotide-activated protein kinase complex	IDA IDA: Inferred from direct assay	21812984	GOA
located in nucleus	IDA IDA: Inferred from direct assay	21085490	GOA
located in perinuclear region of cytoplasm	IDA IDA: Inferred from direct assay	21085490	GOA
located in plasma membrane	IDA IDA: Inferred from direct assay	15569306	GOA
located in plasma membrane raft	IDA IDA: Inferred from direct assay	17911601	GOA
located in sperm midpiece	IDA IDA: Inferred from direct assay	10906071	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

PRKACA Protein Structure

Pkinase

0
100
200
300
351 a.a.

Protein Preferred Names	Protein Names
cAMP-dependent protein kinase catalytic subunit alpha	PKA C-alpha protein kinase A catalytic subunit protein kinase, cAMP-dependent, alpha catalytic subunit protein kinase, cAMP-dependent, catalytic, alpha

PRKACA Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	PRKACA	P17612	PKIB	Homo sapiens	Q9C010-2	Y2H Array	32296183
Intra	PRKACA	P17612	PKIB	Homo sapiens	Q9C010-2	Y2H Prey Pooling	32296183
Intra	PRKACA	P17612	PKIB	Homo sapiens	Q9C010-2	Validated Y2H	32296183
Intra	PRKACA	P17612	APBB2	Homo sapiens	Q92870-2	Y2H Array	32814053
Intra	PRKACA	P17612	APBB2	Homo sapiens	Q92870-2	Y2H Pooling	32814053
Intra	PRKACA	P17612	APBB2	Homo sapiens	Q92870-2	Validated Y2H	32814053
Intra	PRKACA	P17612	PRKAR1B	Homo sapiens	P31321	Protein Kinase Assay	24605759
Intra	PRKACA	P17612	PRKAR1B	Homo sapiens	P31321	Validated Y2H	32296183
Intra	PRKACA	P17612	PRKAR1B	Homo sapiens	P31321	TAP	23455922
Intra	PRKACA	P17612	PRKAR1B	Homo sapiens	P31321	Y2H Array	32296183
Intra	PRKACA	P17612	PRKAR1B	Homo sapiens	P31321	Pull Down	32707033
Intra	PRKACA	P17612	PRKAR1B	Homo sapiens	P31321	Anti Tag CoIP	35271311
Intra	PRKACA	P17612	PRKAR1B	Homo sapiens	P31321	Y2H Prey Pooling	32296183
Intra	PRKACA	P17612	PRKAR2B	Homo sapiens	P31323	TAP	23455922
Intra	PRKACA	P17612	PRKAR2B	Homo sapiens	P31323	Pull Down	32707033
Intra	PRKACA	P17612	PRKAR2B	Homo sapiens	P31323	Anti Tag CoIP	35271311
Intra	PRKACA	P17612	PRKAR2B	Homo sapiens	P31323	Pull Down	17148597
Intra	PRKACA	P17612	PRKAR2B	Homo sapiens	P31323	Validated Y2H	27107012
Intra	PRKACA	P17612	PRKAR2B	Homo sapiens	P31323	Complementation	32296183
Intra	PRKACA	P17612	PRKAR2B	Homo sapiens	P31323	Validated Y2H	32296183
Intra	PRKACA	P17612	PRKAR2B	Homo sapiens	P31323	Protein Kinase Assay	24605759
Intra	PRKACA	P17612	PRKAR2B	Homo sapiens	P31323	BFG-2H	27107012
Intra	PRKACA	P17612	HSP90AA1	Homo sapiens	P07900	TAP	23455922
Intra	PRKACA	P17612	ITGAV	Homo sapiens	P06756	Validated Y2H	32814053
Intra	PRKACA	P17612	ITGAV	Homo sapiens	P06756	Y2H Array	32814053
Intra	PRKACA	P17612	ITGAV	Homo sapiens	P06756	Y2H Pooling	32814053
Intra	PRKACA	P17612	YWHAZ	Homo sapiens	P63104	Protein Kinase Assay	16376338
Intra	PRKACA	P17612	YWHAZ	Homo sapiens	P63104	Anti Tag CoIP	35271311
Intra	PRKACA	P17612	BAG2	Homo sapiens	O95816	Anti Bait CoIP	35167623
Intra	PRKACA	P17612	GSK3B	Homo sapiens	P49841	Anti Bait CoIP	11035810
Intra	PRKACA	P17612	HTT	Homo sapiens	P42858	Y2H Array	32814053
Intra	PRKACA	P17612	HTT	Homo sapiens	P42858	Y2H Pooling	32814053
Intra	PRKACA	P17612	HTT	Homo sapiens	P42858	Validated Y2H	32814053
Intra	PRKACA	P17612	PTN	Homo sapiens	P21246	Validated Y2H	32814053
Intra	PRKACA	P17612	PTN	Homo sapiens	P21246	Y2H Array	32814053
Intra	PRKACA	P17612	PTN	Homo sapiens	P21246	Y2H Pooling	32814053
Intra	PRKACA	P17612	PRKAR1A	Homo sapiens	P10644	TAP	23455922
Intra	PRKACA	P17612	PRKAR1A	Homo sapiens	P10644	Anti Tag CoIP	24855271
Intra	PRKACA	P17612	PRKAR1A	Homo sapiens	P10644	Pull Down	32707033
Intra	PRKACA	P17612	PRKAR1A	Homo sapiens	P10644	Anti Tag CoIP	35271311
Intra	PRKACA	P17612	PRKAR1A	Homo sapiens	P10644	FRET	25477193
Intra	PRKACA	P17612	PRKAR1A	Homo sapiens	P10644	Protein Kinase Assay	24605759
Intra	PRKACA	P17612	AKIP1	Homo sapiens	Q9NQ31	Pull Down	15630084
Intra	PRKACA	P17612	LRRK2	Homo sapiens	Q5S007	Anti Bait CoIP	24464040
Intra	PRKACA	P17612	LRRK2	Homo sapiens	Q5S007	Protein Kinase Assay	24351927
Intra	PRKACA	P17612	PNP	Homo sapiens	P00491	Validated Y2H	32814053
Intra	PRKACA	P17612	PNP	Homo sapiens	P00491	Y2H Array	32814053
Intra	PRKACA	P17612	PNP	Homo sapiens	P00491	Y2H Pooling	32814053
Intra	PRKACA	P17612	DPYSL4	Homo sapiens	O14531	Y2H Pooling	32814053
Intra	PRKACA	P17612	DPYSL4	Homo sapiens	O14531	Validated Y2H	32814053
Intra	PRKACA	P17612	DPYSL4	Homo sapiens	O14531	Y2H Array	32814053
Intra	PRKACA	P17612	CDH1	Homo sapiens	P12830	Validated Y2H	32814053
Intra	PRKACA	P17612	CDH1	Homo sapiens	P12830	Y2H Array	32814053
Intra	PRKACA	P17612	CDH1	Homo sapiens	P12830	Y2H Pooling	32814053
Intra	PRKACA	P17612	ALOX5	Homo sapiens	P09917	Protein Kinase Assay	24860872
Intra	PRKACA	P17612	AVPI1	Homo sapiens	Q5T686	Y2H Prey Pooling	32296183
Intra	PRKACA	P17612	AVPI1	Homo sapiens	Q5T686	Validated Y2H	32296183
Intra	PRKACA	P17612	AVPI1	Homo sapiens	Q5T686	Y2H Array	32296183
Intra	PRKACA	P17612	ATXN1	Homo sapiens	P54253	Validated Y2H	32814053
Intra	PRKACA	P17612	ATXN1	Homo sapiens	P54253	Y2H Array	32814053
Intra	PRKACA	P17612	ATXN1	Homo sapiens	P54253	Y2H Pooling	32814053
Intra	PRKACA	P17612	ATXN3	Homo sapiens	P54252	Y2H Pooling	32814053
Intra	PRKACA	P17612	ATXN3	Homo sapiens	P54252	Validated Y2H	32814053
Intra	PRKACA	P17612	ATXN3	Homo sapiens	P54252	Y2H Array	32814053
Cross	PRKACA	P17612	Prkar2b	Rattus norvegicus	P12369	Anti Bait CoIP	24464040

Cross: Cross-species interaction Intra: Intraspecies interaction

PRKACA Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P810621	Phospho-PKA alpha/beta/gamma (catalytic subunit) (Thr197) Antibody (YA9883)	WB	Human, Mouse, Rat
HY-P81127	PKA alpha+beta Antibody	WB, ICC/IF	Human, Mouse, Rat
HY-P86462	PKA C-alpha Antibody (YA6154)	WB, IHC-P, ICC/IF, IP, ELISA	Human, Mouse, Rat

Related Diseases

Diseases	Alias
Primary Pigmented Nodular Adrenocortical Disease	Ppnad Primary Pigmented Nodular Adrenal Dysplasia Pigmented Nodular Adrenocortical Disease, Primary, 2 Pigmented Nodular Adrenocortical Disease, Primary, 1
Osteoporosis	Postmenopausal Osteoporosis Osteoporosis, Postmenopausal Bone Mineral Density Quantitative Trait Locus Bmnd Osteoporosis, Involutional Osteoporosis, Susceptibility To Osteoporosis, Postmenopausal, Susceptibility Bone Mineral Density Variation Qtl, Osteoporosis OSTEOP Involutional Osteoporosis Senile Osteoporosis Osteoporosis Postmenopausal Bone Mineral Density, Quantitative Trait Locus Osteoporosis, Senile Idiopathic Osteoporosis Bone Rarefaction Nos Type 1 Osteoporosis
Carney Complex Variant	Carney Complex Carney Syndrome Carney Complex, Type 1 Lamb Syndrome Name Syndrome Myxoma-Spotty Pigmentation-Endocrine Overactivity Syndrome Carney Complex - Trismus - Pseudocamptodactyly Syndrome Carney Complex, Type 2 Car Cnc1 Carney Myxoma-Endocrine Complex Myxoma - Spotty Pigmentation - Endocrine Overactivity Myxoma, Spotty Pigmentation, And Endocrine Overactivity Lamb - Lentigines, Atrial Myxoma, Mucocutaneous Myoma, Blue Nevus Syndrome Name - Nevi, Atrial Myxoma, Skin Myxoma, Ephelides Syndrome Carney Complex-Trismus-Pseudocamptodactyly Syndrome CACOV
Cardioacrofacial Dysplasia 1	CAFD1
Lung Cancer	Lung Carcinoma Non-Small Cell Lung Carcinoma Lung Cancer, Protection Against Lung Cancer, Susceptibility To Adenocarcinoma Of Lung, Somatic Nonsmall Cell Lung Cancer Adenocarcinoma Of Lung, Response To Tyrosine Kinase Inhibitor In Lung Neoplasm Carcinoma Of Lung Lung Non-Small Cell Carcinoma Non-Small Cell Lung Cancer Nsclc Lung Neoplasms Malignant Neoplasm Of Lung Alveolar Cell Carcinoma Nonsmall Cell Lung Cancer, Somatic Nonsmall Cell Lung Cancer, Response To Tyrosine Kinase Inhibitor In Nonsmall Cell Lung Cancer, Susceptibility To Lung Cancer, Somatic Lung Cancer, Resistance To Cancer Of Lung Cancer Of Bronchus Cancer Of The Lung Lung Malignancies Lung Malignant Tumors Malignant Lung Tumor Malignant Tumor Of Lung Pulmonary Cancer Pulmonary Carcinoma Pulmonary Neoplasms Respiratory Carcinoma LNCR Adenocarcinoma Of Lung Neoplasm Of Lung Cancer Lung Carcinoma Non-Small Cell Lung Carcinoma, Non-Small-Cell Lung Lung Cancers Lung Carcinomas Cancer, Lung Cancer, Lung, Non-Small Cell Primary Malignant Neoplasm Of Lung Bronchioloalveolar Adenocarcinoma
Breast Cancer	Breast Carcinoma Breast Cancer, Familial Malignant Neoplasm Of Breast Male Breast Cancer Breast Cancer, Susceptibility To Breast Cancer, Early-Onset Malignant Tumor Of Breast Carcinoma Of Male Breast Breast Cancer, Invasive Ductal Breast Cancer, Protection Against Breast Cancer, Somatic Breast Cancer, Male Breast Cancer, Lobular, Somatic Breast Tumor Mammary Cancer Mammary Tumor Malignant Neoplasm Of Male Breast Mammary Carcinoma Male Breast Carcinoma Familial Cancer Of Breast Invasive Ductal Breast Carcinoma Breast Cancer Susceptibility Breast Cancer, Male, Susceptibility To Breast Cancer, Early-Onset, Susceptibility To Malignant Tumor Of The Breast Mammary Neoplasm Primary Breast Cancer Neoplasm Of Male Breast Carcinoma Of Breast Breast Cancer In Men Familial Breast Cancer Cancer Of Breast BC Breast Cancer Familial Breast Cancer Familial Male Breast Cancer, Familial Male Breast Male Carcinoma Breast Neoplasms Breast Neoplasms, Male Mammary Tumors Mammary Carcinomas Cancer, Breast Cancer, Breast, Susceptibility Invasive Breast Ductal Carcinoma Breast Neoplasm Susceptibility To Breast Cancer Mammary Neoplasms Animal Mammary Neoplasms Primary Malignant Neoplasm Of Breast Infiltrating Ductal Carcinoma Of Breast Infiltrating Duct Carcinoma Of Unspecified Site Infiltrating Ductular Carcinoma Of Unspecified Site Invasive Breast Carcinoma Of No Special Type Microinvasive Carcinoma Of Breast Carcinoma With Apocrine Differentiation
Ovarian Cancer	Ovarian Carcinoma Ovarian Neoplasm Malignant Tumour Of Ovary Epithelial Ovarian Cancer Neoplasm Of Ovary Ovarian Neoplasms Ovarian Cancers Malignant Neoplasm Of Ovary Primary Malignant Neoplasm Of Ovary Ovarian Cancer, Somatic Malignant Ovarian Tumor Ovary Neoplasm Primary Ovarian Cancer Tumor Of The Ovary Cancer Of The Ovary Malignant Neoplasm Of The Ovary Malignant Tumor Of The Ovary Ovarian Malignant Tumor OC Ovarian Carcinomas Cancer, Ovarian Cancer Of Ovary Ovary Cancer Ca Ovary
Brachydactyly
Acth-Independent Cushing Syndrome	Adrenal Cushing Syndrome Adrenocorticotropic Hormone-Independent Cushing Syndrome Corticotropin-Independent Cushing Syndrome
Fibrolamellar Carcinoma	Fibrolamellar Hepatocellular Carcinoma Fhcc Fibrolamellar Hepatocarcinoma Hepatocellular Carcinoma, Fibrolamellar Oncocytic Hepatocellular Tumor Eosinophilic Glassy Cell Hepatoma Eosinophilic Hepatocellular Carcinoma With Lamellar Fibrosis Fl-Hcc Fibrolamellar Oncocytic Hepatoma Hepatocellular Carcinoma With Increased Stromal Fibrosis Polygonal Cell Hepatocellular Carcinoma With Fibrous Stroma
Atrioventricular Septal Defect	AVSD Atrioventricular Canal Defect Avcd Endocardial Cushion Defect Ecd Avc Defect Atrioventricular Septal Defect, Susceptibility To, 1 Atrioventricular Septal Defect 1 Endocardial Cushion Defects Septal Defect, Atrioventricular Atrioventricular Defect With Atrial Shunting Only Incomplete Atrioventricular Septal Defect With Isolated Atrial Component Incomplete Atrioventricular Canal Defect With Isolated Atrial Component Primum Atrial Septal Defect Partial Atrioventricular Canal Defect With Isolated Atrial Component Partial Atrioventricular Septal Defect, Ostium Primum Type Ostium Primum Atrial Septal Defect Partial Atrioventricular Canal Defect Partial Atrioventricular Septal Defect Atrial Septum Primum Defect Atrioventricular Canal Defect With Isolated Ventricular Component Atrioventricular Canal Defect With Isolated Ventricular Communication Atrioventricular Septal Defect With Isolated Ventricular Component Atrioventricular Septal Defect With Atrial Shunting And Restrictive Ventricular Shunting Intermediate Atrioventricular Canal Defect With Atrial And Ventricular Components And Separate Atrioventricular Valve Transitional Atrioventricular Septal Defect With Atrial And Ventricular Components And Separate Atrioventricular Valves Atrioventricular Canal Defect Associated With A Restrictive Ventricular Septal Defect Intermediate Atrioventricular Canal Defect Intermediate Atrioventricular Septal Defect With Atrial And Ventricular Components And Separate Atrioventricular Valvar Orifices Intermediate Atrioventricular Septal Defect Transitional Atrioventricular Canal Defect Transitional Atrioventricular Septal Defect Complete Atrioventricular Canal With Atrial And Ventricular Components Complete Atrioventricular Canal Defect Complete Atrioventricular Septal Defect
Pigmented Nodular Adrenocortical Disease, Primary, 4	PPNAD4 Chromosome 19p13 Duplication Syndrome Cushing Syndrome, Adrenal, Due To Ppnad4 Primary Pigmented Nodular Adrenocortical Disease 4 Adrenal Cushing Syndrome Due To Ppnad4 Adrenocortical Disease, Nodular, Pigmented, Primary, Type 4

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (10)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-153967	BLU0588	Inhibitor	97.63%	Unkown
HY-150076	BLU2864	Inhibitor	99.50%	Unkown
HY-150072	DS89002333	Inhibitor	99.85%	Unkown
HY-RS11116	PRKCA Human Pre-designed siRNA Set A		/	Unkown
HY-150072B	(R)-DS89002333	Control	98.74%	Unkown
HY-RS11104	PRKACA Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-150072A	(2S,4R)-DS89002333	Control	/	Unkown
HY-150073	DS01080522	Inhibitor	/	Unkown
HY-RS11105	Prkaca Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS11106	Prkaca Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Canis familiaris	PRKACA	VGNC	VGNC:59441
Felis catus	PRKACA	VGNC	VGNC:69048
Mus musculus	PRKACA	MGD	MGI:97592
Macaca mulatta	PRKACA	VGNC	VGNC:76403
Bos taurus	PRKACA	VGNC	VGNC:50250
Rattus norvegicus	PRKACA	RGD	RGD:3389
Others	PRKACA	NCBI