Gibberellin A4
Based on 1 Customer Validation
Gibberellin A4 is a type of bioactive gibberellin. Gibberellin A4 promotes flowering in apple trees. Gibberellin A4 also exhibits anti-NF-κB and antioxidant activities. Gibberellin A4 can be used in the research of periodontal diseases.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.65%
- CAS. Nr.: 468-44-0
- Formel: C19H24O5
- Molecular Weight:332.39
-
Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biologische Aktivität
-
Chemical Information
-
Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
-
Help & FAQs
-
HY-N7443
Gibberellin A1Plant Hormone -
HY-N1964
Gibberellic acidGrowth Regulator -
HY-125572
Gibberellin A7Plant Hormone -
HY-100560
Abscisic acidPlant Hormone -
HY-W414851
cis-ZeatinBiochemical Reagent -
HY-W011209
N6-IsopentenyladenosineCytokinin Nucleoside/Autophagy Inhibitor -
HY-N0273
BrassinolideApoptosis Inducer -
HY-W011151
trans-ZeatinribosidePlant Growth Regulator -
HY-N0160
KinetinPlant Hormone -
HY-N9418
Gibberellin A5Plant Hormone -
HY-N10568
Gibberellin A9Plant Hormone -
HY-15371
ForskolinAdenylate Cyclase Activator
Biologische Aktivität
Beschreibung
In Vitro
Gibberellin A4 (3-30 μM; 30 min) reduces LPS (HY-D1056)-induced NF-κB activation by 32% in RAW 264.7 macrophages stably transfected with an NF-κB luciferase reporter gene[1].
Gibberellin A4 (3-300 μg, applied 4.5 weeks after flowering) promotes flowering in apple trees[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS. Nr. 468-44-0
-
Appearance Solid
-
Molecular Weight 332.39
-
Formel C19H24O5
-
Color White to off-white
-
SMILES
OC([C@H]1[C@@]2([H])[C@]3(OC([C@@]2([C@H](CC3)O)C)=O)[C@@]4([H])[C@]15C[C@H](C(C5)=C)CC4)=O
-
Structure Classification
-
Initial Source
-
Versand
Room temperature in continental US; may vary elsewhere.
-
Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (300.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.52 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.52 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
-
Data Sheet (279 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
Verweise
[1]. Looney NE, et al. Promotion of flowering in apple trees with gibberellin A4 and C-3 epi-gibberellin A 4. Planta. 1985 Aug;165(2):292-4. [Content Brief]
[2]. Nani BD, et al. A Study on the Anti-NF-κB, Anti-Candida, and Antioxidant Activities of Two Natural Plant Hormones: Gibberellin A4 and A7. Pharmaceutics. 2022;14(7):1347. Published 2022 Jun 25. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0085 mL | 15.0426 mL | 30.0851 mL | 75.2128 mL |
| 5 mM | 0.6017 mL | 3.0085 mL | 6.0170 mL | 15.0426 mL | |
| 10 mM | 0.3009 mL | 1.5043 mL | 3.0085 mL | 7.5213 mL | |
| 15 mM | 0.2006 mL | 1.0028 mL | 2.0057 mL | 5.0142 mL | |
| 20 mM | 0.1504 mL | 0.7521 mL | 1.5043 mL | 3.7606 mL | |
| 25 mM | 0.1203 mL | 0.6017 mL | 1.2034 mL | 3.0085 mL | |
| 30 mM | 0.1003 mL | 0.5014 mL | 1.0028 mL | 2.5071 mL | |
| 40 mM | 0.0752 mL | 0.3761 mL | 0.7521 mL | 1.8803 mL | |
| 50 mM | 0.0602 mL | 0.3009 mL | 0.6017 mL | 1.5043 mL | |
| 60 mM | 0.0501 mL | 0.2507 mL | 0.5014 mL | 1.2535 mL | |
| 80 mM | 0.0376 mL | 0.1880 mL | 0.3761 mL | 0.9402 mL | |
| 100 mM | 0.0301 mL | 0.1504 mL | 0.3009 mL | 0.7521 mL |
Gibberellin A4 Related Classifications
HY-N8440 Related Classifications
Powered by Bioz