Cat. No.: HY-153089

GSK3-IN-3

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

GSK3-IN-3 is a mitophagy inducer, inducing Parkin-dependent mitophagy. GSK3-IN-3 is also a GSK-3 inhibitor with an IC₅₀ value of 3.01 μM. GSK3-IN-3 is non-ATP nor substrate competitive and is neuroprotective against 6-OHDA.

For research use only. We do not sell to patients.

Purity: 99.22%
CAS No.: 331963-27-0
Formula: C₂₄H₃₅N₃O₄
Molecular Weight:429.55

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

GSK3-IN-3 Chemical Structure

CAS No.: 331963-27-0

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

IC50: 3.01 μM (GSK-3)^[3]

All GSK-3 Isoforms :

In Vitro

GSK3-IN-3 (VP07) (25 μM; 24 h) induces mitophagy in Parkin-expressing U2OS-iMLS cells with restricted potency^[1].
GSK3-IN-3 (1.56-25 μM; 24 h) results in mitochondria fission with mitochondrial morphology change in U2OS-iMLS-Parkin cells^[1].
GSK3-IN-3 (VP0.7) (5 μM, 10 μM; ) shows neuroprotection against 6-OHDA albeit in a Parkinson's disease in vitro cellular model in SH-SY5Y cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[1]

Cell Line:

Parkin-expressing U2OS-iMLS cells
Concentration:

1.56 μM, 3.12 μM, 6.25 μM, 12.5 μM, and 25 μM;
Incubation Time:

24 hours
Result:

Induced a mitochondrial morphology change from a filament-shaped network to a more round-shaped network.

Cell Viability Assay^[2]

Cell Line:

SH-SY5Y cells
Concentration:

0.5 μM, 1 μM, 3 μM, 5 μM, and 10 μM
Incubation Time:

16 hours; with 35 μM 6-OHDA
Result:

Inhibited cell growth with an IC₅₀ value of 2.57 μM.

Chemical Information

CAS No. 331963-27-0
Appearance Solid
Molecular Weight 429.55
Formula C₂₄H₃₅N₃O₄
Color Off-white to light yellow
SMILES

O=C(C1=C(N(CC)C2=C(C1=O)C=CC=C2)O)NNC(CCCCCCCCCCC)=O
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (4)

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Cell Death Discov

Mitochondrial dysfunction-mediated metabolic remodeling of TCA cycle promotes Parkinson's disease through inhibition of H3K4me3 demethylation. [Abstract]

2025 Jul 29;11(1):351. PMID: 40730642
Antioxidants (Basel)

Isoquercitrin from Apocynum venetum L. Exerts Antiaging Effects on Yeasts via Stress Resistance Improvement and Mitophagy Induction through the Sch9/Rim15/Msn Signaling Pathway. [Abstract]

2023 Oct 31;12(11):1939. PMID: 38001792
iScience

2025 Dec 8.
J Biol Chem

Activation of Nemo-like Kinase in Diamond Blackfan Anemia suppresses early erythropoiesis by preventing mitochondrial biogenesis. [Abstract]

2024 Aug;300(8):107542. PMID: 38992436

Solvent & Solubility

In Vitro:

DMSO : 4.17 mg/mL (9.71 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3280 mL

11.6401 mL

23.2802 mL

5 mM

0.4656 mL

2.3280 mL

4.6560 mL

10 mM

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Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Maestro I, et al. Phenotypic Assay Leads to Discovery of Mitophagy Inducers with Therapeutic Potential for Parkinson's Disease. ACS Chem Neurosci. 2021 Dec 15;12(24):4512-4523. [Content Brief]

[2]. Morales-García JA, et al. Glycogen synthase kinase-3 inhibitors as potent therapeutic agents for the treatment of Parkinson disease. ACS Chem Neurosci. 2013 Feb 20;4(2):350-60. [Content Brief]

[3]. Palomo V, et al. Exploring the binding sites of glycogen synthase kinase 3. Identification and characterization of allosteric modulation cavities. J Med Chem. 2011 Dec 22;54(24):8461-70. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3280 mL	11.6401 mL	23.2802 mL	58.2004 mL
DMSO	5 mM	0.4656 mL	2.3280 mL	4.6560 mL	11.6401 mL

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GSK3-IN-3

Kinase Inhibitor Library

PI3K/Akt/mTOR Compound Library

Protein Tyrosine Kinase Compound Library

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Reprogramming Compound Library

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Glycolysis Compound Library

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Neuroprotective Compound Library

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Anti-Cancer Metabolism Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

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Antidepressant Compound Library

Cancer Stem Cells Compound Library

Metabolic Enzyme Compound Library

Serine/Threonine Kinase Inhibitor Library

Mitophagy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) GSK3-IN-3

All GSK-3 Isoforms