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Cat. No.: HY-P991584

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HuGAL-FR21

Name: HuGAL-FR21
Brand: MedChemExpress
SKU: HY-P991584
Price: 480.00 USD
Availability: InStock
Rating: 5 (1 reviews)

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HuGAL-FR21 is a humanized antiFGFR2IIIb IgG1 monoclonal antibody. HuGAL-FR21 can block the binding of FGF2, FGF7, and FGF10 to FGFR2IIIb and inhibit FGF-induced phosphorylation of FGFR2IIIb. HuGAL-FR21 can downregulate the expression of FGFR2 in SNU-16 cells. HuGAL-FR21 shows the significant anti-tumor activity in athymic nude mice bearing gastric cancer xenograft models. HuGAL-FR21 can be used for research on cancer such as gastric cancer.

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Pureté: 97.80%
Masse moléculaire:143.79 kDa

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

HuGAL-FR21 Chemical Structure

Size

Prix

Stock

Quantité

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vitro
- In Vivo
- Gene ID
- Accession
- Conjugated
- Reconsititution
- Format
- Application
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Isotype

Human IgG1 kappa

Recommend Isotype Controls

Human IgG1 kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

^[1]

FGFR2

In Vitro

HuGAL-FR21 (0.001-1 μg/mL) can specifically bind to FGFR2IIIb (EC₅₀ approximately 20 pM) and its mutant S252W in SNU-16 cells^[1].
HuGAL-FR21 (0.01-100 μg/mL) effectively blocks the binding of FGFR2IIIb (IC₅₀ approximately 1.5-3 μg/mL) to FGF2, FGF7, and FGF10^[1].
HuGAL-FR21 (10 μg/mL, 30 min) effectively blocks FGF7 induced phosphorylation and significantly inhibits FGF2 induced phosphorylation in SNU-16 cells^[1].
HuGAL-FR21 (10 μg/mL, 2-24 h) downregulates FGFR2 protein levels in SNU-16 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

SNU-16 cells
Concentration:

10 μg/mL
Incubation Time:

2, 8 and 48 h
Result:

Reduced the level of FGFR2 protein by approximately 50%.

In Vivo

HuGAL-FR21 (0.5-5 mg/kg; i.p.; twice weekly; for 25-30 days) effectively inhibits the growth of tumors and has no obvious toxicity in athymic nude mice bearing SNU-16 xenografts or OCUM-2M xenografts^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

5×10⁶ SNU-16 or OCUM-2M cells injected female athymic nude mice (5-6 weeks)^[1]
Dosage:

0.5, 1, 2.5 and 5 mg/kg in SNU-16 model, 1 and 2.5 mg/kg in OCUM-2M model
Administration:

Intraperitoneal injection (i.p.); twice weekly for 25 days in SNU-16 model and 30 days in OCUM-2M model
Result:

Significantly inhibited the growth of SNU-16 and OCUM-2M tumors.
Did not show any other obvious toxicity.

Gene ID

2263 [NCBI]

Accession

P21802

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Format

Product Image

Application

ELISA, FACS, Functional assay

Chemical Information

Appearance Liquid
Masse moléculaire 143.79 kDa
Color Colorless to light yellow
SMILES

N/A
Livraison

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (260 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Inhibitory Antibodies User Guide (603 KB)

Références

[1]. Zhao WM, et al. Monoclonal antibodies to fibroblast growth factor receptor 2 effectively inhibit growth of gastric tumor xenografts. Clin Cancer Res. 2010 Dec 1;16(23):5750-8. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Keywords

MOQ

Minimum order quantity

100 mg

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