iCRT3
Based on 11 publication(s) in Google Scholar
iCRT3 is an inhibitor of both Wnt and β-catenin-responsive transcription.
For research use only. We do not sell to patients.
- Purity: 98.05%
- CAS No.: 901751-47-1
- Formula: C23H26N2O2S
- Molecular Weight:394.53
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Immunology/Inflammation Compound Library
HY-L007
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Stem Cell Signaling Compound Library
HY-L017
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Wnt/Hedgehog/Notch Compound Library
HY-L020
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Anti-Cancer Compound Library
HY-L025
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Anti-Aging Compound Library
HY-L034
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Differentiation Inducing Compound Library
HY-L038
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Pyroptosis Compound Library
HY-L059
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Cytoskeleton Compound Library
HY-L060
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Neuroprotective Compound Library
HY-L070
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Anti-Breast Cancer Compound Library
HY-L074
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Anti-Liver Cancer Compound Library
HY-L101
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Anti-Colorectal Cancer Compound Library
HY-L103
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Anti-Prostate Cancer Compound Library
HY-L124
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Anti-Pulmonary Fibrosis Compound Library
HY-L125
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Cancer Stem Cells Compound Library
HY-L135
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Heterocyclic Compound Library
HY-L138
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Antihypertensive Compound Library
HY-L145
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Cell Death Library
HY-L162
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Extracellular Vesicles (EVs) Compound Library
HY-L168
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Anti-Gastric Cancer Compound Library
HY-L184
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Anti-Fibrosis Compound Library
HY-L185
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
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Anti-Rheumatic Arthritis Compound Library
HY-L210
Publications Citing Use of MedChemExpress (MCE) iCRT3
More- Nat Mater. 2026 Feb 20.
- Nat Commun. 2022 Nov 2;13(1):6552. [Abstract]
- Nat Commun. 2022 Jul 28;13(1):4364. [Abstract]
- Nat Commun. 2021 Nov 24;12(1):6831. [Abstract]
- Acta Pharm Sin B. 2023 May;13(5):2259-2268. [Abstract]
- BMC Biol. 2023 May 4;21(1):100. [Abstract]
- BMC Biol. 2020 Oct 27;18(1):151. [Abstract]
- IUBMB Life. 2023 Jul;75(7):609-623. [Abstract]
- Cancer Med. 2024 May;13(9):e7221. [Abstract]
- bioRxiv. 2025 April 10.
- Research Square Print. 2023 Feb 3.
Customer Validation & Images
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RT-PCR
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
2.4 μM
Compound: 8, iCRT3
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Inhibition of interaction of beta-catenin (unknown origin) transfected in Wnt-activated HEK293 cells/Tcf4 assessed as decrease in transactivation of canonical Wnt signaling by TOPflash luciferase reporter assay
Inhibition of interaction of beta-catenin (unknown origin) transfected in Wnt-activated HEK293 cells/Tcf4 assessed as decrease in transactivation of canonical Wnt signaling by TOPflash luciferase reporter assay
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[PMID: 25985283] |
| SW480 | IC50 |
1.1 μM
Compound: 8, iCRT3
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Inhibition of beta-catenin/Tcf4 interaction in human SW480 cells assessed as decrease in transactivation of canonical Wnt signaling by TOPflash luciferase reporter assay
Inhibition of beta-catenin/Tcf4 interaction in human SW480 cells assessed as decrease in transactivation of canonical Wnt signaling by TOPflash luciferase reporter assay
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[PMID: 25985283] |
iCRT3 is an inhibitor of both Wnt and β-catenin-responsive transcription. iCRT3 significantly decreases TOP Flash activity and reduces the level of NTSR1. The anti-apoptotic effects of Neurotensin (NTS) and Wnt3a can be largely abrogated by iCRT3[1]. Cells maintained long term with iCRT3 show enhanced expression of classic pluripotency genes compare with the DMSO control, whereas expression of differentiation markers and T-cell factor (TCF) target genes is concomitantly reduced[2]. Treatment with iCRT3 at doses of 12.5, 25, 50, and 75 μM decreases TNF-α levels by 14.7%, 18.5%, 44.9% and 61.3%, respectively. With iCRT3 treatment, IκB levels are increased in a dose-dependent manner compare to the vehicle[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 901751-47-1
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Appearance Solid
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Molecular Weight 394.53
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Formula C23H26N2O2S
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Color White to off-white
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SMILES
O=C(NCCC1=CC=CC=C1)CSCC2=C(C)OC(C3=CC=C(CC)C=C3)=N2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (11)
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Journal Impact Factor
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Most Recent
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Nat Commun
Stromal androgen signaling acts as tumor niches to drive prostatic basal epithelial progenitor-initiated oncogenesis. [Abstract]2022 Nov 2;13(1):6552. PMID: 36323713 -
Nat Commun
Aberrant androgen action in prostatic progenitor cells induces oncogenesis and tumor development through IGF1 and Wnt axes. [Abstract]2022 Jul 28;13(1):4364. PMID: 35902588 -
Nat Commun
2021 Nov 24;12(1):6831. PMID: 34819502 -
Acta Pharm Sin B
Berberine improves central memory formation of CD8+ T cells: Implications for design of natural product-based vaccines. [Abstract]2023 May;13(5):2259-2268. PMID: 37250162 -
BMC Biol
aristaless1 has a dual role in appendage formation and wing color specification during butterfly development. [Abstract]2023 May 4;21(1):100. PMID: 37143075 -
BMC Biol
Hyperactivated Wnt-β-catenin signaling in the absence of sFRP1 and sFRP5 disrupts trophoblast differentiation through repression of Ascl2. [Abstract]2020 Oct 27;18(1):151. PMID: 33109217 -
IUBMB Life
2-Deoxy-D-glucose simultaneously targets glycolysis and Wnt/β-catenin signaling to inhibit cervical cancer progression. [Abstract]2023 Jul;75(7):609-623. PMID: 36809563
iCRT3 purchased from MedChemExpress. Usage Cited in: IUBMB Life. 2023 Jul;75(7):609-623. [Abstract]
iCRT3 (10 ug/mL; 48 h) significantly lowers mRNA levels of glycolytic genes (Fig 4), as well as reduces glucose uptake (Fig i) and lactate production (Fig j) in HeLa cells.
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Cancer Med
Long non-coding RNA NEAT1 promotes aerobic glycolysis and progression of cervical cancer through WNT/β-catenin/PDK1 axis. [Abstract]2024 May;13(9):e7221. PMID: 38733179 -
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Solvent & Solubility
DMSO : 150 mg/mL (380.20 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.34 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Cells are seeded into 96-well plates to a density of 5×103 cells per well and incubated in the culture medium with iCRT3 for an additional 48 h. Cell viability and cell apoptosis assays are carried out using a Cell Counting kit-8 and a Caspase-Glo 3/7 assay kit according to the manufacturer’s instructions, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
NOD-SCID BALB/c mice are inoculated subcutaneously in the right back with 2×106 A172 cells. The growth of the primary tumors is recorded every 4 days. iCRT3 (5 mg/kg) is diluted in PBS i.p. triweekly when tumors grow to ~200 mm3. The control mice are treated with blank PBS containing 5% (v/v) DMSO. Tumor volume is evaluated with the following formula: volume=tumor length×width2/2. The mice are sacrificed 24 days after pharmaceutical treatment. The tumors are resected and embedded in paraffin, and the Ki67 staining is analyzed by immunohistochemistry[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
[1]. Xiao H, et al. A Novel Positive Feedback Loop Between NTSR1 and Wnt/β-Catenin Contributes to Tumor Growth of Glioblastoma. Cell Physiol Biochem. 2017 Oct 24;43(5):2133-2142. [Content Brief]
[2]. Chatterjee SS, et al. Inhibition of β-catenin-TCF1 interaction delays differentiation of mouse embryonic stem cells. J Cell Biol. 2015 Oct 12;211(1):39-51. [Content Brief]
[3]. Sharma A, et al. Mitigation of sepsis-induced inflammatory responses and organ injury through targeting Wnt/β-catenin signaling. doi: 10.1038/s41598-017-08711-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5347 mL | 12.6733 mL | 25.3466 mL | 63.3665 mL |
| 5 mM | 0.5069 mL | 2.5347 mL | 5.0693 mL | 12.6733 mL | |
| 10 mM | 0.2535 mL | 1.2673 mL | 2.5347 mL | 6.3367 mL | |
| 15 mM | 0.1690 mL | 0.8449 mL | 1.6898 mL | 4.2244 mL | |
| 20 mM | 0.1267 mL | 0.6337 mL | 1.2673 mL | 3.1683 mL | |
| 25 mM | 0.1014 mL | 0.5069 mL | 1.0139 mL | 2.5347 mL | |
| 30 mM | 0.0845 mL | 0.4224 mL | 0.8449 mL | 2.1122 mL | |
| 40 mM | 0.0634 mL | 0.3168 mL | 0.6337 mL | 1.5842 mL | |
| 50 mM | 0.0507 mL | 0.2535 mL | 0.5069 mL | 1.2673 mL | |
| 60 mM | 0.0422 mL | 0.2112 mL | 0.4224 mL | 1.0561 mL | |
| 80 mM | 0.0317 mL | 0.1584 mL | 0.3168 mL | 0.7921 mL | |
| 100 mM | 0.0253 mL | 0.1267 mL | 0.2535 mL | 0.6337 mL |
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