24-Hydroxycholesterol
Based on 3 publication(s) in Google Scholar
24-Hydroxycholesterol is a natural sterol, which serves as a positive allosteric modulator of N-Methyl-d-Aspartate (NMDA) receptorsR, and a potent activator of the transcription factors LXR.
For research use only. We do not sell to patients.
- Purity: 99.90%
- CAS No.: 30271-38-6
- Formula: C27H46O2
- Molecular Weight:402.65
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Membrane Transporter/Ion Channel Compound Library
HY-L011
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Publications Citing Use of MedChemExpress (MCE) 24-Hydroxycholesterol
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Customer Validation & Images
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IF
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WB
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IHC
All iGluR Isoforms
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Biological Activity
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NMDA Receptor |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 30271-38-6
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Appearance Solid
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Molecular Weight 402.65
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Formula C27H46O2
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Color White to off-white
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SMILES
CC(C)C(O)CC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
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Structure Classification
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Initial Source
brain
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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ACS Nano
Immune Modulatory Oxysterols Produced from Cholesterol-Containing Lipid Nanoparticles Regulate Tumor Growth. [Abstract]2026 Mar 13. PMID: 41825961 -
Cell Chem Biol
Transient hydroxycholesterol treatment restrains TCR signaling to promote long-term immunity. [Abstract]2024 May 16;31(5):920-931.e6. PMID: 38759618 -
PLoS Biol
The cholesterol 24-hydroxylase CYP46A1 promotes α-synuclein pathology in Parkinson's disease. [Abstract]2025 Feb 18;23(2):e3002974. PMID: 39964974
24-Hydroxycholesterol purchased from MedChemExpress. Usage Cited in: PLoS Biol. 2025 Feb 18;23(2):e3002974. [Abstract]
Representative immunostaining and quantification of the insoluble α-Syn inclusions (green) and pS129 (red) in the presence or absence of 24-Hydroxycholesterol (24-OHC) (30 μM) for 48 h.
24-Hydroxycholesterol purchased from MedChemExpress. Usage Cited in: PLoS Biol. 2025 Feb 18;23(2):e3002974. [Abstract]
Immunoblots of phosphorylated α-Syn (pS129) and α-Syn in the presence or absence of 24-Hydroxycholesterol (24-OHC) (30 μM) for 48 h.
24-Hydroxycholesterol purchased from MedChemExpress. Usage Cited in: PLoS Biol. 2025 Feb 18;23(2):e3002974. [Abstract]
Representative immunohistochemistry images showing ipsilateral pS129-positive LB-like accumulation after PK digestion treated with 24-Hydroxycholesterol (24-OHC) (100 μg/kg).
Solvent & Solubility
Ethanol : 60 mg/mL (149.01 mM; Need ultrasonic)
DMSO : 3.67 mg/mL (9.11 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.21 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.21 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
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- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Soncini M, et al. 24-Hydroxycholesterol participates in pancreatic neuroendocrine tumor development. Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):E6219-E6227. Epub 2016 Sep 26. [Content Brief]
[2]. Mast N, et al. Cholesterol-metabolizing enzyme cytochrome P450 46A1 as a pharmacologic target for Alzheimer's disease. Neuropharmacology. 2017 Sep 1;123:465-476. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / Ethanol | 1 mM | 2.4835 mL | 12.4177 mL | 24.8355 mL | 62.0887 mL |
| 5 mM | 0.4967 mL | 2.4835 mL | 4.9671 mL | 12.4177 mL | |
| Ethanol | 10 mM | 0.2484 mL | 1.2418 mL | 2.4835 mL | 6.2089 mL |
| 15 mM | 0.1656 mL | 0.8278 mL | 1.6557 mL | 4.1392 mL | |
| 20 mM | 0.1242 mL | 0.6209 mL | 1.2418 mL | 3.1044 mL | |
| 25 mM | 0.0993 mL | 0.4967 mL | 0.9934 mL | 2.4835 mL | |
| 30 mM | 0.0828 mL | 0.4139 mL | 0.8278 mL | 2.0696 mL | |
| 40 mM | 0.0621 mL | 0.3104 mL | 0.6209 mL | 1.5522 mL | |
| 50 mM | 0.0497 mL | 0.2484 mL | 0.4967 mL | 1.2418 mL | |
| 60 mM | 0.0414 mL | 0.2070 mL | 0.4139 mL | 1.0348 mL | |
| 80 mM | 0.0310 mL | 0.1552 mL | 0.3104 mL | 0.7761 mL | |
| 100 mM | 0.0248 mL | 0.1242 mL | 0.2484 mL | 0.6209 mL |
24-Hydroxycholesterol Related Classifications
HY-N2370 Related Classifications
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