Thapsigargin
Based on 142 publication(s) in Google Scholar
Thapsigargin, an endoplasmic reticulum (ER) stress inducer, is an inhibitor of microsomal Ca2+-ATPase. Thapsigargin efficiently inhibits coronavirus (HCoV-229E, MERS-CoV, SARS-CoV-2) replication in different cell types.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 67526-95-8
- Formula: C34H50O12
- Molecular Weight:650.75
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
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Publications Citing Use of MedChemExpress (MCE) Thapsigargin
More- Signal Transduct Target Ther. 2025 Dec 17;10(1):413. [Abstract]
- Signal Transduct Target Ther. 2024 Dec 30;9(1):367. [Abstract]
- Nature. 2026 Apr;652(8108):209-219. [Abstract]
- Nature. 2025 Oct;646(8086):913-924. [Abstract]
- Cell. 2024 Apr 25;187(9):2288-2304.e27. [Abstract]
- Cell Metab. 2025 Dec 29:S1550-4131(25)00530-3. [Abstract]
- Nat Immunol. 2024 Jan;25(1):54-65. [Abstract]
- Cancer Commun (Lond). 2025 Nov 10. [Abstract]
- Nat Neurosci. 2024 Aug;27(8):1522-1533. [Abstract]
- Nat Microbiol. 2025 Nov;10(11):2949-2965. [Abstract]
- Nat Cell Biol. 2025 Dec;27(12):2143-2151. [Abstract]
- ACS Nano. 2024 Jan 23;18(3):2162-2183. [Abstract]
- ACS Nano. 2021 Jun 22;15(6):10640-10658. [Abstract]
- Nat Commun. 2026 Apr 22;17(1):5596. [Abstract]
- Nat Commun. 2024 Aug 9;15(1):6810. [Abstract]
- Nat Commun. 2023 Nov 1;14(1):6982. [Abstract]
- Neuron. 2026 Mar 11:S0896-6273(26)00086-3. [Abstract]
- Acta Pharm Sin B. 2023 Sep;13(9):3744-3755. [Abstract]
- Autophagy. 2025 Apr;21(4):840-859. [Abstract]
- Autophagy. 2025 Jan;21(1):178-190. [Abstract]
- Adv Sci (Weinh). 2025 Sep 12:e09588. [Abstract]
- Adv Sci (Weinh). 2025 May 31:e03505. [Abstract]
- Adv Sci (Weinh). 2024 Sep 19:e2403442. [Abstract]
- Adv Sci (Weinh). 2022 Oct 10;e2203831. [Abstract]
- Adv Sci (Weinh). 2020 Oct 4;7(22):2002747. [Abstract]
- Nat Chem Biol. 2025 May 26. [Abstract]
- Carbohydr Polym. 2022 Feb 1:277:118869. [Abstract]
- Redox Biol. 2024 Feb:69:102983. [Abstract]
- Appl Phys Rev. 2026 Jan 21.
- J Hazard Mater. 2024 Mar 5:465:133038. [Abstract]
- Cancer Lett. 2021 Nov 1:520:26-37. [Abstract]
- Cell Death Dis. 2024 Dec 18;15(12):890. [Abstract]
- Cell Death Dis. 2020 Oct 13;11(10):847. [Abstract]
- Genes Dis. 2021 May 15;9(6):1689-1700. [Abstract]
- Proc Natl Acad Sci U S A. 2025 Sep 2;122(35):e2513155122. [Abstract]
- Int J Biol Macromol. 2024 Nov;281(Pt 2):136314. [Abstract]
- Int J Biol Macromol. 2022 Aug 31:215:23-35. [Abstract]
- Int J Biol Macromol. 2020 Dec 1;164:2409-2417. [Abstract]
- Acta Pharmacol Sin. 2023 Apr;44(4):811-821. [Abstract]
- Phytomedicine. 2025 Jan 28:139:156431. [Abstract]
- EMBO Mol Med. 2022 Jan 11;14(1):e14502. [Abstract]
- Free Radic Biol Med. 2025 Jun:233:148-161. [Abstract]
- Free Radic Biol Med. 2021 Dec:177:156-166. [Abstract]
- ACS Appl Mater Interfaces. 2019 Oct 16;11(41):38190-38204. [Abstract]
- Apoptosis. 2020 Dec;25(11-12):853-863. [Abstract]
- Neoplasia. 2025 Apr 18:65:101165. [Abstract]
- NPJ Breast Cancer. 2022 Jan 10;8(1):2. [Abstract]
- Biomed Pharmacother. 2023 Feb:158:114133. [Abstract]
- Cell Chem Biol. 2025 Jun 19;32(6):854-871.e6. [Abstract]
- Cell Death Discov. 2025 Apr 6;11(1):146. [Abstract]
- Cell Death Discov. 2025 Apr 10;11(1):162. [Abstract]
- Cell Rep. 2026 Mar 12;45(3):117083. [Abstract]
- Cell Rep. 2026 Jan 3;45(1):116683. [Abstract]
- Cell Rep. 2025 Nov 25;44(11):116502. [Abstract]
- Cell Rep. 2024 Jun 27;43(7):114402. [Abstract]
- Cell Rep. 2022 Nov 8;41(6):111599. [Abstract]
- Antioxidants (Basel). 025 Jan 24;14(2):137. [Abstract]
- Comput Biol Med. 2025 Oct 18;198(Pt B):111215. [Abstract]
- Ecotoxicol Environ Saf. 2024 Jan 15:270:115831. [Abstract]
- J Mol Cell Biol. 2025 Dec 5:mjaf050. [Abstract]
- J Mol Cell Biol. 2025 Jul 28;17(2):mjaf002. [Abstract]
- Cell Biol Toxicol. 2023 Jun;39(3):907-928. [Abstract]
- Bioeng Transl Med. 2025 Dec 5.
- Biochem Pharmacol. 2025 Dec;242(Pt 4):117377. [Abstract]
- Cell Prolif. 2021 Nov;54(11):e13133. [Abstract]
- J Ethnopharmacol. 2026 May 10:362:121364. [Abstract]
- CNS Neurosci Ther. 2025 Nov;31(11):e70620. [Abstract]
- Inflammation. 2025 Oct;48(5):3146-3155. [Abstract]
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- Nutrients. 2021 Dec 1;13(12):4343. [Abstract]
- Int J Mol Sci. 2025 Apr 10;26(8):3556. [Abstract]
- Chin J Nat Med. 2021 Apr;19(4):255-266. [Abstract]
- Int Immunopharmacol. 2025 Dec 11:169:116010. [Abstract]
- Int Immunopharmacol. 2025 Sep 13:165:115532. [Abstract]
- Eur J Pharmacol. 2025 May 23:177765. [Abstract]
- Int Immunopharmacol. 2024 Sep 16;142(Pt B):113116. [Abstract]
- Invest Ophthalmol Vis Sci. 2024 May 1;65(5):8. [Abstract]
- FASEB J. 2025 Nov 30;39(22):e71250. [Abstract]
- Poult Sci. 2024 Jun;103(6):103656. [Abstract]
- iScience. 2026 Jan 27;29(3):114818. [Abstract]
- iScience. 2025 Nov 30.
- Comput Struct Biotechnol J. 2023 Sep 9:21:4478-4487. [Abstract]
- Am J Physiol Heart Circ Physiol. 2020 Dec 1;319(6):H1482-H1495. [Abstract]
- Cell Calcium. 2026 Mar:134:103122. [Abstract]
- Cell Calcium. 2026 Mar:134:103117. [Abstract]
- J Neurosci. 2024 Nov 26:e0607242024. [Abstract]
- J Cell Physiol. 2023 Mar;238(3):582-596. [Abstract]
- Cell Calcium. 2022 Jun;104:102571. [Abstract]
- Fish Shellfish Immunol. 2024 Nov 9:155:110020. [Abstract]
- Biomedicines. 2024 Apr 18;12(4):902. [Abstract]
- Biomedicines. 2023 Jun 4;11(6):1637. [Abstract]
- Aquaculture. 2023 Jun 8, 739757.
- Virol J. 2025 Nov 17;22(1):378. [Abstract]
- Microbiol Spectr. 2022 Dec 21;10(6):e0328222. [Abstract]
- J Virol. 2022 Feb 9;96(3):e0110321. [Abstract]
- Biochim Biophys Acta Mol Cell Res. 2026 Jun;1873(5):120164. [Abstract]
- Cell Signal. 2025 Jun:130:111702. [Abstract]
- World J Stem Cells. 2025 Nov 26;17(11):112393. [Abstract]
- Heliyon. 2024 Feb 23;10(5):e26904. [Abstract]
- Vet Res. 2024 Aug 2;55(1):97. [Abstract]
- J Neuroimmune Pharmacol. 2024 Jan 12;19(1):1. [Abstract]
- RNA Biol. 2025 Dec;22(1):1-12. [Abstract]
- J Bioenerg Biomembr. 2026 Feb 11;58(1):1. [Abstract]
- J Bioenerg Biomembr. 2026 Feb 12;58(1):2. [Abstract]
- Curr Issues Mol Biol. 2026 Jan 23;48(2):125. [Abstract]
- J Bioenerg Biomembr. 2025 Jul 28. [Abstract]
- Arch Biochem Biophys. 2024 Jun:756:110009. [Abstract]
- Biol Reprod. 2023 Jul 11;109(1):53-64. [Abstract]
- Front Vet Sci. 2021 Jul 6:8:645571. [Abstract]
- Mol Biol Rep. 2023 Aug;50(8):6457-6468. [Abstract]
- Vet Microbiol. 2026 May:316:110968. [Abstract]
- Vet Microbiol. 2024 May:292:110051. [Abstract]
- Mol Biol Cell. 2024 Jan 1;35(1):ar5. [Abstract]
- Vet Microbiol. 2019 Dec;239:108485. [Abstract]
- Addict Biol. 2024 Mar;29(3):e13382. [Abstract]
- Int J Colorectal Dis. 2025 Aug 21;40(1):184. [Abstract]
- Biochem Biophys Res Commun. 2025 Nov 1:787:152776. [Abstract]
- Biomol Biomed. 2024 Dec 11;25(1):210-225. [Abstract]
- Oncol Lett. 2024 May 28;28(2):339. [Abstract]
- Diagn Microbiol Infect Dis. 2024 Aug 22;110(4):116500. [Abstract]
- Res Sq. 2026 May 24.
- bioRxiv. 2026 Mar 25.
- bioRxiv. 2026 Mar 30:2026.03.28.714855. [Abstract]
- bioRxiv. 2026 Feb 24.
- bioRxiv. 2026 Jan 13.
- bioRxiv. 2026 Jan 11:2026.01.09.698733. [Abstract]
- bioRxiv. 2026 Jan 28.
- Res Sq. 2026 Jan 9.
- The Medical College of Wisconsin. 2025.
- SSRN. 2025 Sep 30.
- bioRxiv. 2025 Aug 21:2025.08.15.670603. [Abstract]
- University of Bonn. 2025 Jul 4.
- bioRxiv. 2025 April 03.
- Res Sq. 2025 Feb 11:rs.3.rs-4138879. [Abstract]
- Drexel University. 2025.
- Research Square Preprint. 2024 Dec 04.
- Res Sq. 2024 Nov 10.
- J Tradit Chinese Medical Sci. 2024 Jun 5.
- Preprints. 2024 Jan 30.
- Research Square Preprint. 2023 Jul 28.
- Research Square Print. September 9th, 2022.
- Oxid Med Cell Longev. 2021 Jan 4;2021:8836818. [Abstract]
Customer Validation & Images
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WB
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RT-PCR
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WB
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Cell Imaging/Staining
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Flow Cytometry
All Calcium Channel Isoforms
More
Biological Activity
Ca2+-ATPase[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CCRF S-180 | IC50 |
30 nM
Compound: 3; TPG
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Cytotoxicity against mouse CCRF S-180 cells incubated for 48 hrs by sulforhodamine B staining based ELISA
Cytotoxicity against mouse CCRF S-180 cells incubated for 48 hrs by sulforhodamine B staining based ELISA
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[PMID: 33289552] |
| CCRF-CEM | IC50 |
0.27 μM
Compound: 6
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Cytotoxicity against human CCRF-CEM cells assessed as cell viability after 48 hrs by celltiter-blue assay
Cytotoxicity against human CCRF-CEM cells assessed as cell viability after 48 hrs by celltiter-blue assay
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[PMID: 22582991] |
| EL4 | IC50 |
0.1 μM
Compound: 3; TPG
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Cytotoxicity against mouse EL4 cells incubated for 48 hrs by sulforhodamine B staining based ELISA
Cytotoxicity against mouse EL4 cells incubated for 48 hrs by sulforhodamine B staining based ELISA
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[PMID: 33289552] |
| EL4 | IC50 |
1.4 μM
Compound: thapsigargin
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Cytotoxicity against mouse EL4 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Cytotoxicity against mouse EL4 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25951057] |
| HL-60 | IC50 |
0.007 μM
Compound: 6
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Cytotoxicity against human HL60 cells assessed as cell viability after 48 hrs by celltiter-blue assay
Cytotoxicity against human HL60 cells assessed as cell viability after 48 hrs by celltiter-blue assay
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[PMID: 22582991] |
| K562 | IC50 |
0.17 μM
Compound: 6
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Cytotoxicity against human K562 cells assessed as cell viability after 48 hrs by celltiter-blue assay
Cytotoxicity against human K562 cells assessed as cell viability after 48 hrs by celltiter-blue assay
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[PMID: 22582991] |
| MCF-10A | IC50 |
2.4 μM
Compound: thapsigargin
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Cytotoxicity against human MCF10A cells assessed as growth inhibition
Cytotoxicity against human MCF10A cells assessed as growth inhibition
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[PMID: 25951057] |
| MCF7 | GI50 |
5 μM
Compound: Thapsigargin
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Cytotoxicity against human MCF7 cells after 24 hrs by CellTiter-Glo assay
Cytotoxicity against human MCF7 cells after 24 hrs by CellTiter-Glo assay
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[PMID: 30528127] |
| MCF7 | IC50 |
2.3 μM
Compound: 3; TPG
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Cytotoxicity against human MCF7 cells incubated for 48 hrs by sulforhodamine B staining based ELISA
Cytotoxicity against human MCF7 cells incubated for 48 hrs by sulforhodamine B staining based ELISA
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[PMID: 33289552] |
| MCF7 | IC50 |
2.7 μM
Compound: thapsigargin
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Cytotoxicity against human MCF7 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Cytotoxicity against human MCF7 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25951057] |
| PC-3 | IC50 |
2.4 μM
Compound: thapsigargin
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Cytotoxicity against human PC3 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Cytotoxicity against human PC3 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25951057] |
Thapsigargin (0.001- 1 μM; for 2 and 4 days) arrests cell proliferations in MH7A human rheumatoid arthritis synovial cells in a time- and dose-dependent manner[2].
Thapsigargin (0.001- 1 μM; for 2 and 4 days) induces cell apoptosis in MH7A cells in a time- and dose-dependent manner[2].
Thapsigargin (0.001- 1 μM; for 2 and 4 days) impairs mTOR activity and leads to cyclin D1 expressions in MH7A cells[2].
Thapsigargin inhibits Ca2+ entry into human neutrophil granulocytes[1].
Thapsigargin inhibits the carbachol-evoked [Ca2+]i-transients with (IC50=0.353 nM) or without (IC50=0.448 nM) a KCl-prestimulation, but an additional small component, with a much lower sensitivity (IC50=4814 nM), is observed in the absence of a KCl-prestimulation. In contrast, the KCl-evoked [Ca2+]i-transients displayed only one component with a very low sensitivity to Thapsigargin in both absence (IC50=3343 nM) and presence (IC50=6858 nM) of a carbachol-prestimulation[3].
Thapsigargin also phosphorylate p38 MAPK by Ca2+ influx through SOCE, leading to suppression of TNF-α-induced NF-κB phosphorylation[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MH7A human rheumatoid arthritis synovial cells
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Concentration:0.001, 0.1, and 1 μM
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Incubation Time:For 2 and 4 days
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Result:Arrested cell proliferations in a time- and dose-dependent manner.
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Cell Line:MH7A human rheumatoid arthritis synovial cells
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Concentration:0.001, 0.1, and 1 μM
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Incubation Time:For 2 and 4 days
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Result:Induces cell apoptosis in a time- and dose-dependent manner.
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Cell Line:MH7A human rheumatoid arthritis synovial cells
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Concentration:0.001, 0.1, and 1 μM
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Incubation Time:For 2 and 4 days
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Result:Impairs mTOR activity and leads to cyclin D1 expressions
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Balb/c mice (20-25 g)[4]
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Dosage:0.25 ug/g, 0.5 ug/g and 1 ug/g
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Administration:Injection; 24 hours
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Result:Increased of 2 to 5-fold in chemokine and pro-inflammatory expression.
Chemical Information
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CAS No. 67526-95-8
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Appearance Solid
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Molecular Weight 650.75
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Formula C34H50O12
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Color White to off-white
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SMILES
CCCCCCCC(O[C@@H]1[C@@H](OC(/C(C)=C\C)=O)C(C)=C2[C@@]1([H])[C@@](C)(OC(C)=O)C[C@H](OC(CCC)=O)[C@@]([C@@]3(O)C)(O)[C@@]2([H])OC3=O)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (142)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Disruption of heme homeostasis by nuclear receptor Nur77 induces pyroptosis through granzyme B-dependent GSDMC cleavage. [Abstract]2025 Dec 17;10(1):413. PMID: 41407678 -
Signal Transduct Target Ther
2024 Dec 30;9(1):367. PMID: 39737965
Thapsigargin purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 30;9(1):367. [Abstract]
Tunicamycin (0.1 μM; 18 h) significantly reduces the protein abundance of IRF2BP2 in LPS-stimulated macrophages in a BiP-dependent manner.
Thapsigargin purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 30;9(1):367. [Abstract]
Tunicamycin (0.1 μM; 18 h) doesn't decrease the mRNA levels of Irf2bp2 in LPS-stimulated macrophages.
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Nature
2026 Apr;652(8108):209-219. PMID: 41781621 -
Nature
2025 Oct;646(8086):913-924. PMID: 40769205 -
Cell
Cancer SLC6A6-mediated taurine uptake transactivates immune checkpoint genes and induces exhaustion in CD8+ T cells. [Abstract]2024 Apr 25;187(9):2288-2304.e27. PMID: 38565142
Thapsigargin purchased from MedChemExpress. Usage Cited in: Cell. 2024 Apr 25;187(9):2288-2304.e27. [Abstract]
Thapsigargin (10 nM) increases p-PERK, p-eIF2α, and ATF4 expressions as well as CD8+ T cell death and dysfunction in human CD8+ T cells.
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Cell Metab
2025 Dec 29:S1550-4131(25)00530-3. PMID: 41468885 -
Nat Immunol
Oleic acid availability impacts thymocyte preprogramming and subsequent peripheral Treg cell differentiation. [Abstract]2024 Jan;25(1):54-65. PMID: 38062135 -
Cancer Commun (Lond)
Unfolded protein response kinase PERK supports survival and metastasis of circulating tumor cell clusters via SAM synthesis and H3K4me3-dependent PDGFB signaling. [Abstract]2025 Nov 10. PMID: 41212905 -
Nat Neurosci
Spatiotemporally selective astrocytic ATP dynamics encode injury information sensed by microglia following brain injury in mice. [Abstract]2024 Aug;27(8):1522-1533. PMID: 38862791 -
Nat Microbiol
Mycobacterium tuberculosis-derived linoleic acid increases regulatory T cell function to promote bacterial survival within macrophages. [Abstract]2025 Nov;10(11):2949-2965. PMID: 41073667 -
Nat Cell Biol
DNA fragmentation factor B suppresses interferon to enable cancer persister cell regrowth. [Abstract]2025 Dec;27(12):2143-2151. PMID: 41249572 -
ACS Nano
Ligand Phase Separation-Promoted, "Squeezing-Out" Mode Explaining the Mechanism and Implications of Neutral Nanoparticles That Escaped from Lysosomes. [Abstract]2024 Jan 23;18(3):2162-2183. PMID: 38198577 -
ACS Nano
Polyethylenimine-Modified Mesoporous Silica Nanoparticles Induce a Survival Mechanism in Vascular Endothelial Cells via Microvesicle-Mediated Autophagosome Release. [Abstract]2021 Jun 22;15(6):10640-10658. PMID: 34080832 -
Nat Commun
Transcription factor 19 modulates fatty acid elongation and unfolded protein response to attenuate palmitic acid-induced hepatic dysfunction. [Abstract]2026 Apr 22;17(1):5596. PMID: 42020413 -
Nat Commun
Super enhancer acquisition drives expression of oncogenic PPP1R15B that regulates protein homeostasis in multiple myeloma. [Abstract]2024 Aug 9;15(1):6810. PMID: 39122682 -
Nat Commun
Hepatocyte FBXW7-dependent activity of nutrient-sensing nuclear receptors controls systemic energy homeostasis and NASH progression in male mice. [Abstract]2023 Nov 1;14(1):6982. PMID: 37914694 -
Neuron
Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes. [Abstract]2026 Mar 11:S0896-6273(26)00086-3. PMID: 41819100 -
Acta Pharm Sin B
Translocation of IGF-1R in endoplasmic reticulum enhances SERCA2 activity to trigger Ca2+ER perturbation in hepatocellular carcinoma. [Abstract]2023 Sep;13(9):3744-3755. PMID: 37719369 -
Autophagy
CKAP4 in hepatocellular carcinoma: competitive RETREG1/FAM134B binding, reticulophagy regulation, and cancer progression. [Abstract]2025 Apr;21(4):840-859. PMID: 39689859 -
Autophagy
TRIM21-mediated ubiquitination of SQSTM1/p62 abolishes its Ser403 phosphorylation and enhances palmitic acid cytotoxicity. [Abstract]2025 Jan;21(1):178-190. PMID: 39172027 -
Adv Sci (Weinh)
XBP1s Mediates Cross-resistance to Combination Treatment of CDK4/6 Inhibitors plus Endocrine Therapy in Breast Cancer. [Abstract]2025 Sep 12:e09588. PMID: 40940685 -
Adv Sci (Weinh)
2025 May 31:e03505. PMID: 40448612 -
Adv Sci (Weinh)
siRNA/CS-PLGA Nanoparticle System Targeting Knockdown Intestinal SOAT2 Reduced Intestinal Lipid Uptake and Alleviated Obesity. [Abstract]2024 Sep 19:e2403442. PMID: 39297413 -
Adv Sci (Weinh)
The Endoplasmic Reticulum ATP13A1 is Essential for MAVS-Mediated Antiviral Innate Immunity. [Abstract]2022 Oct 10;e2203831. PMID: 36216581 -
Adv Sci (Weinh)
Long Noncoding RNA HITTERS Protects Oral Squamous Cell Carcinoma Cells from Endoplasmic Reticulum Stress-Induced Apoptosis via Promoting MRE11-RAD50-NBS1 Complex Formation. [Abstract]2020 Oct 4;7(22):2002747. PMID: 33240783 -
Nat Chem Biol
2025 May 26. PMID: 40419770 -
Carbohydr Polym
Chitosan oligosaccharide regulates AMPK and STAT1 pathways synergistically to mediate PD-L1 expression for cancer chemoimmunotherapy. [Abstract]2022 Feb 1:277:118869. PMID: 34893274 -
Redox Biol
Shank3 ameliorates neuronal injury after cerebral ischemia/reperfusion via inhibiting oxidative stress and inflammation. [Abstract]2024 Feb:69:102983. PMID: 38064762 -
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J Hazard Mater
A regulatory module comprising G3BP1-FBXL5-IRP2 axis determines sodium arsenite-induced ferroptosis. [Abstract]2024 Mar 5:465:133038. PMID: 38118197 -
Cancer Lett
NCAPD2 inhibits autophagy by regulating Ca2+/CAMKK2/AMPK/mTORC1 pathway and PARP-1/SIRT1 axis to promote colorectal cancer. [Abstract]2021 Nov 1:520:26-37. PMID: 34229059 -
Cell Death Dis
NIR-715 photodynamic therapy induces immunogenic cancer cell death by enhancing the endoplasmic reticulum stress response. [Abstract]2024 Dec 18;15(12):890. PMID: 39695072 -
Cell Death Dis
PERK controls bone homeostasis through the regulation of osteoclast differentiation and function. [Abstract]2020 Oct 13;11(10):847. PMID: 33051453 -
Genes Dis
Intranuclear cardiac troponin I plays a functional role in regulating Atp2a2 expression in cardiomyocytes. [Abstract]2021 May 15;9(6):1689-1700. PMID: 36157491 -
Proc Natl Acad Sci U S A
2025 Sep 2;122(35):e2513155122. PMID: 40880539 -
Int J Biol Macromol
GCRV-encoded circRNA circ_20 forms a ternary complex with BIP and PERK to delay virus replication by inhibiting the PERK-eIF2α pathway. [Abstract]2024 Nov;281(Pt 2):136314. PMID: 39370064 -
Int J Biol Macromol
Investigation of the internalization and transport mechanism of Codonopsis Radix polysaccharide both in mice and Caco-2 cells. [Abstract]2022 Aug 31:215:23-35. PMID: 35718143 -
Int J Biol Macromol
Trop2 inhibition of P16 expression and the cell cycle promotes intracellular calcium release in OSCC. [Abstract]2020 Dec 1;164:2409-2417. PMID: 32768481 -
Acta Pharmacol Sin
TRPV4 channel is involved in HSV-2 infection in human vaginal epithelial cells through triggering Ca2+ oscillation. [Abstract]2023 Apr;44(4):811-821. PMID: 36151392 -
Phytomedicine
Palmatine activation of TFEB enhances autophagy and alleviates endoplasmic reticulum stress in intervertebral disc degeneration. [Abstract]2025 Jan 28:139:156431. PMID: 39933468 -
EMBO Mol Med
Carfilzomib modulates tumor microenvironment to potentiate immune checkpoint therapy for cancer. [Abstract]2022 Jan 11;14(1):e14502. PMID: 34898004 -
Free Radic Biol Med
Autophagy and Endoplasmic Reticulum Stress-Related Protein Homeostasis Links Palmitic Acid to Hepatic Lipotoxicity in Zebrafish (Danio rerio), Counteracted by Linoleic Acid. [Abstract]2025 Jun:233:148-161. PMID: 40089081 -
Free Radic Biol Med
High-fat diet aggravates colitis-associated carcinogenesis by evading ferroptosis in the ER stress-mediated pathway. [Abstract]2021 Dec:177:156-166. PMID: 34688836 -
ACS Appl Mater Interfaces
Effect of PEGylated Magnetic PLGA-PEI Nanoparticles on Primary Hippocampal Neurons: Reduced Nanoneurotoxicity and Enhanced Transfection Efficiency with Magnetofection. [Abstract]2019 Oct 16;11(41):38190-38204. PMID: 31550131 -
Apoptosis
Sphingosylphosphorylcholine alleviates hypoxia-caused apoptosis in cardiac myofibroblasts via CaM/p38/STAT3 pathway. [Abstract]2020 Dec;25(11-12):853-863. PMID: 33068199 -
Neoplasia
2025 Apr 18:65:101165. PMID: 40252311 -
NPJ Breast Cancer
Endoplasmic reticulum stress inhibits AR expression via the PERK/eIF2α/ATF4 pathway in luminal androgen receptor triple-negative breast cancer and prostate cancer. [Abstract]2022 Jan 10;8(1):2. PMID: 35013318 -
Biomed Pharmacother
Jianpi-Qingchang decoction alleviates ulcerative colitis by modulating endoplasmic reticulum stress-related autophagy in intestinal epithelial cells. [Abstract]2023 Feb:158:114133. PMID: 36521243 -
Cell Chem Biol
Mechanism by which the molecular glue-like verteporfin induces IRE1α dimerization and activation to synergize with AKT inhibition in breast cancer. [Abstract]2025 Jun 19;32(6):854-871.e6. PMID: 40472846 -
Cell Death Discov
Neuronal CDK5RAP3 deficiency leads to encephalo-dysplasia via upregulation of N-glycosylases and glycogen deposition. [Abstract]2025 Apr 6;11(1):146. PMID: 40188151 -
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Thapsigargin purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Oct;48(5):3146-3155. [Abstract]
The melanocytes were treated with TM (3 μM), thapsigargin (1 μM), or DTT (1 mM) for 24 h to induce ER stress.
Thapsigargin purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Oct;48(5):3146-3155. [Abstract]
The melanocytes were treated with TM (3 μM), thapsigargin (1 μM), or DTT (1 mM) for 24 h to induce ER stress.
Thapsigargin purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Oct;48(5):3146-3155. [Abstract]
The melanocytes were treated with TM (3 μM), thapsigargin (1 μM), or DTT (1 mM) for 24 h to induce ER stress. ER stress induced an increase in the apoptosis of melanocytes.
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Thapsigargin purchased from MedChemExpress. Usage Cited in: Microbiol Spectr. 2022 Dec 21;10(6):e0328222. [Abstract]
Both PK-15 and 3D4/21cells are transfected with MGF110-7L-expressing plasmids with an increasing dose or empty vector and treated with Thapsigargin (TG) as positive controls. TG significant increases the levels of phosphorylated eIF2a (P-eIF2a) and ATF4 protein in the cells.
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Solvent & Solubility
DMSO : 50 mg/mL (76.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% Saline
Solubility: 5 mg/mL (7.68 mM); Suspended solution; Need ultrasonic
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.20 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (558 KB)
- English - EN (558 KB)
- Français - FR (558 KB)
- Deutsch - DE (558 KB)
- Norwegian - NO (558 KB)
- Español - ES (558 KB)
- Swedish - SV (558 KB)
- Italian - IT (558 KB)
- Portuguese - PT (558 KB)
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Handling Instructions (2659 KB)
References
[1]. Geiszt M, et al. Thapsigargin inhibits Ca2+ entry into human neutrophil granulocytes. Biochem J. 1995 Jan 15;305 ( Pt 2):525-8. [Content Brief]
[2]. Wang H, et al. Effects of thapsigargin on the proliferation and survival of human rheumatoid arthritis synovialcells. ScientificWorldJournal. 2014 Feb 9;2014:605416. [Content Brief]
[3]. Garavito-Aguilar ZV, et al. Differential thapsigargin-sensitivities and interaction of Ca2+ stores in human SH-SY5Y neuroblastoma cells. Brain Res. 2004 Jun 18;1011(2):177-86. [Content Brief]
[4]. Abdullahi A, et al. Modeling Acute ER Stress in Vivo and in Vitro. Shock. 2017 Apr;47(4):506-513. [Content Brief]
[6]. Junsuke Uwada, et al. Store-operated calcium entry (SOCE) contributes to phosphorylation of p38 MAPK and suppression of TNF-α signalling in the intestinal epithelial cells. Cell Signal. 2019 Nov;63:109358. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5367 mL | 7.6834 mL | 15.3669 mL | 38.4172 mL |
| 5 mM | 0.3073 mL | 1.5367 mL | 3.0734 mL | 7.6834 mL | |
| 10 mM | 0.1537 mL | 0.7683 mL | 1.5367 mL | 3.8417 mL | |
| 15 mM | 0.1024 mL | 0.5122 mL | 1.0245 mL | 2.5611 mL | |
| 20 mM | 0.0768 mL | 0.3842 mL | 0.7683 mL | 1.9209 mL | |
| 25 mM | 0.0615 mL | 0.3073 mL | 0.6147 mL | 1.5367 mL | |
| 30 mM | 0.0512 mL | 0.2561 mL | 0.5122 mL | 1.2806 mL | |
| 40 mM | 0.0384 mL | 0.1921 mL | 0.3842 mL | 0.9604 mL | |
| 50 mM | 0.0307 mL | 0.1537 mL | 0.3073 mL | 0.7683 mL | |
| 60 mM | 0.0256 mL | 0.1281 mL | 0.2561 mL | 0.6403 mL |
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