Cat. No.: HY-N2195

Nootkatone

(Synonyms: (+)-Nootkatone)

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Nootkatone, a neuroprotective agent from Vitis vinifera, has antioxidant and anti-inflammatory effects. Nootkatone improves cognitive impairment in lipopolysaccharide-induced mouse model of Alzheimer's disease.

For research use only. We do not sell to patients.

Purity: 99.81%
CAS No.: 4674-50-4
Formula: C₁₅H₂₂O
Molecular Weight:218.33

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Nootkatone Chemical Structure

CAS No.: 4674-50-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Nootkatone, a neuroprotective agent from Vitis vinifera, has antioxidant and anti-inflammatory effects^[1]. Nootkatone improves cognitive impairment in lipopolysaccharide-induced mouse model of Alzheimer's disease^[2].

All TNF Receptor Isoforms :

All Interleukin Related Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Macrophage	IC₅₀	34 μM Compound: 4	Inhibition of LPS-induced NO production in ddY mouse macrophages assessed as nitrate accumulation after 20 hrs by Griess reagent method Inhibition of LPS-induced NO production in ddY mouse macrophages assessed as nitrate accumulation after 20 hrs by Griess reagent method	[PMID: 12398545]

In Vivo

Nootkatone (10 mg/kg) group shows good performance in behavior experiments including Y-maze test and Morris water maze test. The results of histopathological examination and immunohistochemical analysis showed that LPS induced degeneration of neurons and activation of microglia particularly in hippocampus and Nootkatone (10 mg/kg) reversed these changes. Enzyme linked immunosorbent assay and western blot analysis also demonstrated that the model group had increased expression of IL-1β, IL-6, TNF-α, NLRP3 and NF-κB p65, especially in hippocampus relative to sham-operated group, and Nootkatone (10 mg/kg) decreased the high expression of these inflammatory cytokines^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 4674-50-4
Appearance Solid
Molecular Weight 218.33
Formula C₁₅H₂₂O
Color White to yellow
SMILES

O=C1C=C2CC[C@@H](C(C)=C)C[C@@]2(C)[C@H](C)C1
Synonyms

(+)-Nootkatone
Structure Classification
- Terpenoids
- Sesquiterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (4)

All Cell Proliferation/Viability AssayIFCell Migration/Invasion AssayFlow CytometryWB

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Journal Impact Factor

Mol Med

Nootkatone inhibits the progression of glioblastoma by activating the ATF4-CHOP-CHAC1 pathway. [Abstract]

2025 Jan 16;31(1):13. PMID: 39819316

Nootkatone purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Jan 16;31(1):13. [Abstract]

The cell viability of C6 cells and U251 cells treated with gradient concentrations (0 µM, 100 µM, 200 µM, 300 µM) of Nootkatone detected by CCK8 assays.

Nootkatone purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Jan 16;31(1):13. [Abstract]

Representative images of PH3 staining (red) in C6 cells after being treated with DMSO or 200 µM Nootkatone for 48 h.

Nootkatone purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Jan 16;31(1):13. [Abstract]

Representative images of C6 cells and U251 cells treated with 200 µM Nootkatone (NKT) in wound healing assays.

Nootkatone purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Jan 16;31(1):13. [Abstract]

Quantitative analysis in C6 cells and U251 cells after Nootkatone (NKT) (200 μM) treatment for 48 h.

Nootkatone purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Jan 16;31(1):13. [Abstract]

The expression of ATF4, CHOP, CHAC1 in U251 cells was detected after being treated with 200 µM Nootkatone (NKT) for 48 h by western blot.
Environ Pollut

Enantioselective metabolic mechanism and metabolism pathway of tetraconazole in human liver microsomes: In vitro and in silico study. [Abstract]

2026 May 1:396:127849. PMID: 41720237
PLoS Biol

A high-throughput behavioral screening platform for measuring chemotaxis by C. elegans. [Abstract]

2024 Jun 27;22(6):e3002672. PMID: 38935621
bioRxiv

An efficient behavioral screening platform classifies natural products and other chemical cues according to their chemosensory valence in C. elegans. [Abstract]

2024 Apr 3:2023.06.02.542933. PMID: 37333363

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (458.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.5802 mL

22.9011 mL

45.8022 mL

5 mM

0.9160 mL

4.5802 mL

9.1604 mL

10 mM

0.4580 mL

2.2901 mL

4.5802 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (11.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (11.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (11.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wang Y, et al. Nootkatone, a neuroprotective agent from Alpiniae Oxyphyllae Fructus, improves cognitive impairment in lipopolysaccharide-induced mouse model of Alzheimer's disease. Int Immunopharmacol. 2018 Sep;62:77-85. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.5802 mL	22.9011 mL	45.8022 mL	114.5056 mL
	5 mM	0.9160 mL	4.5802 mL	9.1604 mL	22.9011 mL
	10 mM	0.4580 mL	2.2901 mL	4.5802 mL	11.4506 mL
	15 mM	0.3053 mL	1.5267 mL	3.0535 mL	7.6337 mL
	20 mM	0.2290 mL	1.1451 mL	2.2901 mL	5.7253 mL
	25 mM	0.1832 mL	0.9160 mL	1.8321 mL	4.5802 mL
	30 mM	0.1527 mL	0.7634 mL	1.5267 mL	3.8169 mL
	40 mM	0.1145 mL	0.5725 mL	1.1451 mL	2.8626 mL
	50 mM	0.0916 mL	0.4580 mL	0.9160 mL	2.2901 mL
	60 mM	0.0763 mL	0.3817 mL	0.7634 mL	1.9084 mL
	80 mM	0.0573 mL	0.2863 mL	0.5725 mL	1.4313 mL
	100 mM	0.0458 mL	0.2290 mL	0.4580 mL	1.1451 mL

Keywords

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Nootkatone

Antioxidant Compound Library

Differentiation Inducing Compound Library

Medicine Food Homology Compound Library

Terpenoids Library

Pyroptosis Compound Library

Traditional Chinese Medicine Active Compound Library

Anti-Alzheimer's Disease Compound Library

Neuroprotective Compound Library

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Membrane Receptor-targeted Compound Library

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Radioprotector Library

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MCE Bioactive Compound Library

Natural Product Library Plus

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Miao Ethnicity Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

Flavor Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

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Mass Spectrometry Human Metabolite Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Flavors and Fragrances Compound Library

Natural Product Diversity Scaffold Library

Tonifying Traditional Chinese Medicine Monomer Compound Library

Mass Spectrometry Natural Product Library

Natural Product and Natural Product-Like Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Natural Product Library

Anti-Cancer Compound Library

Publications Citing Use of MedChemExpress (MCE) Nootkatone

All TNF Receptor Isoforms

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Nootkatone Related Classifications

HY-N2195 Related Classifications