Cat. No.: HY-N0777

Isorhamnetin-3-O-glucoside

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

Isorhamnetin-3-O-glucoside is an orally active natural compound. Isorhamnetin 3-O-glucoside increases P-ERK, ERK, P-Akt (Ser473), P-PI3K, and PDX-1. Isorhamnetin 3-O-glucoside downregulates C/EBPα and inhibits lipase. Isorhamnetin 3-O-glucoside reduces lipids and inhibits obesity.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 5041-82-7
Formula: C₂₂H₂₂O₁₂
Molecular Weight:478.40

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Isorhamnetin-3-O-glucoside Chemical Structure

CAS No.: 5041-82-7

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All ERK Isoforms :

All Akt Isoforms :

All PI3K Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
BMDC	IC₅₀	>50 μM Compound: 11	Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BMDC	IC₅₀	>50 μM Compound: 11	Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BMDC	IC₅₀	>50 μM Compound: 11	Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
C2C12	EC₅₀	18.5 μM Compound: 6	Increase in glucose uptake in mouse C2C12 cells incubated for 18 hrs using [3H]-2-deoxy-D-glucose by liquid scintillation counting Increase in glucose uptake in mouse C2C12 cells incubated for 18 hrs using [3H]-2-deoxy-D-glucose by liquid scintillation counting	[PMID: 22738356]
Monocyte	IC₅₀	3.8 x 10^-6 M Compound: isorhamnetin 3-glucoside	Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs	[PMID: 8882428]

In Vitro

Isorhamnetin 3-O-glucoside (100 μM; 2 h) significantly increases insulin secretion in INS-1 rat insulin-secreting β-cells^[2].
Isorhamnetin-3-O-glucoside (10-500 μM) inhibits lipase in a concentration-dependent manner in the lipase inhibitory activity assay^[3].
Isorhamnetin-3-O-glucoside (250 μM; during differentiation (8 days in total)) significantly inhibits lipid accumulation in 3T3-L1 preadipocytes^[3].
Isorhamnetin-3-O-glucosylrhamnoside (24 h) shows an inhibitory effect on the viability of HT-29 RFP cells, with an IC₅₀ value of 53.72 μg/mL^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Isorhamnetin-3-O-glucoside (50 mg/kg; p.o.; daily; 20 days) significantly reduces body weight gain, feed efficiency, and epididymal tissue weight in db/db obesity mice, and also decreases LDL levels and slightly reduces total cholesterol levels^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 5041-82-7
Appearance Solid
Molecular Weight 478.40
Formula C₂₂H₂₂O₁₂
Color Light yellow to yellow
SMILES

O=C1C(O[C@@H]([C@@H]([C@@H](O)[C@@H]2O)O)O[C@@H]2CO)=C(C3=CC(OC)=C(O)C=C3)OC4=CC(O)=CC(O)=C14
Structure Classification
- Flavonoids
- Flavonols
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (5)

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Journal Impact Factor

Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 Oct 15:489:144992. PMID: 40466530
Ind Crops Prod

2026 May 8;246:123392.
Ind Crops Prod

2025 Dec 11;239:122458.
Food Biosci

2026 Apr 15;80:108917.

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (522.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0903 mL

10.4515 mL

20.9030 mL

5 mM

0.4181 mL

2.0903 mL

4.1806 mL

10 mM

0.2090 mL

1.0452 mL

2.0903 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Le-yue Du, et al. Analysis of the Metabolites of Isorhamnetin 3-O-Glucoside Produced by Human Intestinal Flora in Vitro by Applying Ultraperformance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry. J Agric Food Chem. 201462122489-2495.

[2]. Lee D, et al. In vitro studies to assess the α-glucosidase inhibitory activity and insulin secretion effect of isorhamnetin 3-o-glucoside and quercetin 3-o-glucoside isolated from Salicornia herbacea. Processes, 2021, 9(3): 483.

[3]. Lee J H, et al. The beneficial effect of Salicornia herbacea extract and isorhamnetin-3-O-glucoside on obesity. Processes, 2023, 11(4): 977.

[4]. Wang H, et al. Structures, Sources, Identification/Quantification Methods, Health Benefits, Bioaccessibility, and Products of Isorhamnetin Glycosides as Phytonutrients. Nutrients. 2023 Apr 18;15(8):1947. [Content Brief]

[5]. Antunes-Ricardo M, et al. Opuntia ficus-indica Extract and Isorhamnetin-3-O-Glucosyl-Rhamnoside Diminish Tumor Growth of Colon Cancer Cells Xenografted in Immune-Suppressed Mice through the Activation of Apoptosis Intrinsic Pathway. Plant Foods Hum Nutr. 2021 Dec;76(4):434-441. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0903 mL	10.4515 mL	20.9030 mL	52.2575 mL
	5 mM	0.4181 mL	2.0903 mL	4.1806 mL	10.4515 mL
	10 mM	0.2090 mL	1.0452 mL	2.0903 mL	5.2258 mL
	15 mM	0.1394 mL	0.6968 mL	1.3935 mL	3.4838 mL
	20 mM	0.1045 mL	0.5226 mL	1.0452 mL	2.6129 mL
	25 mM	0.0836 mL	0.4181 mL	0.8361 mL	2.0903 mL
	30 mM	0.0697 mL	0.3484 mL	0.6968 mL	1.7419 mL
	40 mM	0.0523 mL	0.2613 mL	0.5226 mL	1.3064 mL
	50 mM	0.0418 mL	0.2090 mL	0.4181 mL	1.0452 mL
	60 mM	0.0348 mL	0.1742 mL	0.3484 mL	0.8710 mL
	80 mM	0.0261 mL	0.1306 mL	0.2613 mL	0.6532 mL
	100 mM	0.0209 mL	0.1045 mL	0.2090 mL	0.5226 mL

Keywords

Isorhamnetin-3-O-glucoside

5 Cited Publications

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Isorhamnetin-3-O-glucoside

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Publications Citing Use of MedChemExpress (MCE) Isorhamnetin-3-O-glucoside

All ERK Isoforms

All Akt Isoforms

All PI3K Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Isorhamnetin-3-O-glucoside Related Classifications

HY-N0777 Related Classifications