JAK2 JH2 binder-1
Based on 1 Customer Validation
JAK2 JH2 binder-1 (compound 11) is a potent and selective JAK2 JH2 binder, with a Kd of 37.1 nM. JAK2 JH2 binder-1 has the potential for various myeloproliferative neoplasms research.
For research use only. We do not sell to patients.
- Purity: 95.40%
- CAS No.: 2923309-41-3
- Formula: C29H25N7O6S
- Molecular Weight:599.62
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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JAK2 JH2 37.1 nM (Kd) |
JAK2 JH2 binder-1 (20 μM, 1-48 hours) completely inhibits STAT5 phosphorylation, effective in both V617F and wild-type JAK2 expressing cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEL cell line (V617F JAK2 mutant, erythroleukemia) and TF-1 cell line (wild-type JAK2, cytokine-dependent)
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Concentration:2 to 30 μM
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Incubation Time:1, 3, 24, 48 h
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Result:Completely inhibited STAT5 phosphorylation in HEL and TF-1 cells
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mouse xenograft model (transplanted with HEL cells in C.B-17 SCID-beige mice)[1]
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Dosage:25 mg/kg
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Administration:Intravenous injection (i.v.), 3 times per week for 3 weeks
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Result:Significant reduced tumor volume and inhibited tumor growth.
Chemical Information
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CAS No. 2923309-41-3
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Appearance Solid
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Molecular Weight 599.62
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Formula C29H25N7O6S
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Color White to off-white
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SMILES
NC1=NC(NC2=CC=C(C=C2)S(=O)(N)=O)=NN1C(NC3=CC=C(C=C3)C4=CC(C(O)=O)=C(C=C4)OCC5=CC=CC=C5)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (83.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (4.17 mM); Clear solution; Need ultrasonic and warming and heat to 80°C
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6677 mL | 8.3386 mL | 16.6772 mL | 41.6931 mL |
| 5 mM | 0.3335 mL | 1.6677 mL | 3.3354 mL | 8.3386 mL | |
| 10 mM | 0.1668 mL | 0.8339 mL | 1.6677 mL | 4.1693 mL | |
| 15 mM | 0.1112 mL | 0.5559 mL | 1.1118 mL | 2.7795 mL | |
| 20 mM | 0.0834 mL | 0.4169 mL | 0.8339 mL | 2.0847 mL | |
| 25 mM | 0.0667 mL | 0.3335 mL | 0.6671 mL | 1.6677 mL | |
| 30 mM | 0.0556 mL | 0.2780 mL | 0.5559 mL | 1.3898 mL | |
| 40 mM | 0.0417 mL | 0.2085 mL | 0.4169 mL | 1.0423 mL | |
| 50 mM | 0.0334 mL | 0.1668 mL | 0.3335 mL | 0.8339 mL | |
| 60 mM | 0.0278 mL | 0.1390 mL | 0.2780 mL | 0.6949 mL | |
| 80 mM | 0.0208 mL | 0.1042 mL | 0.2085 mL | 0.5212 mL |
JAK2 JH2 binder-1 Related Classifications
HY-146186 Related Classifications
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