CK-666
Based on 29 publication(s) in Google Scholar
CK-666 is a cell-permeable actin-related protein Arp2/3 complex inhibitor (IC50=12 μM). CK-666 binds to Arp2/3 complex, stabilizes the inactive state of the complex, blocking movement of the Arp2 and Arp3 subunits into the activated filament-like (short pitch) conformation.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 442633-00-3
- Formula: C18H17FN2O
- Molecular Weight:296.34
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Anti-Infection Compound Library
HY-L002
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HY-L025
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HY-L181
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) CK-666
More- Cell. 2025 Dec 11;188(25):7155-7174.e25. [Abstract]
- J Extracell Vesicles. 2026 Mar;15(3):e70248. [Abstract]
- J Extracell Vesicles. 2025 Aug;14(8):e70146. [Abstract]
- Autophagy. 2026 Jun 3:1-31. [Abstract]
- Nat Commun. 2026 May 4. [Abstract]
- Nat Commun. 2025 Dec 12;16(1):11137. [Abstract]
- Nat Commun. 2025 Aug 5;16(1):7190. [Abstract]
- Adv Sci (Weinh). 2026 Mar;13(16):e13310. [Abstract]
- Adv Sci (Weinh). 2025 Dec 27:e13632. [Abstract]
- Adv Sci (Weinh). 2025 Sep 17:e00358. [Abstract]
- Cell Death Dis. 2026 Mar 30;17(1):434. [Abstract]
- Cell Death Dis. 2020 Sep 15;11(9):757. [Abstract]
- Small. 2021 Nov 5;e2104328. [Abstract]
- Neural Regen Res. 2025 Oct 30. [Abstract]
- NPJ Parkinsons Dis. 2024 Apr 9;10(1):80. [Abstract]
- Cell Rep. 2025 Mar 25;44(4):115462. [Abstract]
- Curr Biol. 2025 Apr 7;35(7):1612-1621.e3. [Abstract]
- J Ethnopharmacol. 2021 Jan 10;264:113206. [Abstract]
- Life Sci. 2025 Mar 29:123593. [Abstract]
- Int Immunopharmacol. 2025 Aug 25:164:115401. [Abstract]
- iScience. 2021 Dec 25;25(1):103676. [Abstract]
- FASEB J. 2025 May 31;39(10):e70660. [Abstract]
- Reproduction. 2026 Jan 15;171(1):xaaf013. [Abstract]
- Transbound Emerg Dis. 2025 Nov 21:2025:5822897. [Abstract]
- Res Sq. 2026 Apr 26.
- bioRxiv. 2025 Aug 12.
- Nanyang Technological University. 2024.
- bioRxiv. 2024 October 02.
- bioRxiv. 2024 August 26.
Customer Validation & Images
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Cell Imaging/Staining
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2D/3D Cell Culture and Differentiation
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Cell Migration/Invasion Assay
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In Vivo Efficacy Study
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WB
Biological Activity
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HIV-1 |
CK-666 (100 μM) significantly reduces the number of filopodia on the cell surface of treated trabecular meshwork (TM) cells as well as reduces the length of those filopodia that are present[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 442633-00-3
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Appearance Solid
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Molecular Weight 296.34
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Formula C18H17FN2O
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Color White to off-white
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SMILES
O=C(NCCC1=C(C)NC2=C1C=CC=C2)C3=CC=CC=C3F
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (29)
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Journal Impact Factor
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Most Recent
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Cell
Innate immune and metabolic signals induce mitochondria-dependent membrane lysis via mitoxyperiosis. [Abstract]2025 Dec 11;188(25):7155-7174.e25. PMID: 41317732 -
J Extracell Vesicles
Neuronal Extracellular Vesicles Carrying APOE Downregulate Filament Actin Polymerization Signaling to Inhibit Synapse Formation in Alzheimer's Disease. [Abstract]2026 Mar;15(3):e70248. PMID: 41806350 -
J Extracell Vesicles
Exomeres From Adventitial Fibroblasts of Spontaneously Hypertensive Rats Promote Vascular Remodelling via Transferring Osteopontin. [Abstract]2025 Aug;14(8):e70146. PMID: 40767027
CK-666 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Aug;14(8):e70146. [Abstract]
CK666 (25 μM, 24.5 h) reduced the uptake of S-EMs by VSMCs.
CK-666 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Aug;14(8):e70146. [Abstract]
CK666 (25 μM, 24.5 h) inhibited the S-EMs-induced proliferation of VSMCs from normotensive Wistar-Kyoto rats and spontaneously hypertensive rats.
CK-666 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Aug;14(8):e70146. [Abstract]
CK666 (25 μM, 24.5 h) inhibited the S-EMs-induced migration of VSMCs from normotensive Wistar-Kyoto rats and spontaneously hypertensive rats.
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Autophagy
WNT2B impairs endosomal trafficking via WASHC5 to inhibit autophagy: a novel non-secretory WNT pathway. [Abstract]2026 Jun 3:1-31. PMID: 42233622 -
Nat Commun
AAK1-mediated phosphorylation of PDLIM5 and Talin1 promotes focal adhesion disassembly to accelerate cell migration. [Abstract]2026 May 4. PMID: 42082516 -
Nat Commun
G-CSF/NAMPT signaling drives neutrophil dysfunction and enhances bacterial infection susceptibility in cancer patients. [Abstract]2025 Dec 12;16(1):11137. PMID: 41387444 -
Nat Commun
Promoting implant osseointegration via the osteoblast-selective β-amino acid polymer strategy. [Abstract]2025 Aug 5;16(1):7190. PMID: 40764473 -
Adv Sci (Weinh)
2026 Mar;13(16):e13310. PMID: 41589654 -
Adv Sci (Weinh)
Low YTHDC1 Expression Upregulates FSCN1 to Promote Nuclear F-Actin Formation and Facilitate Double-strand DNA Breaks Repair in TMZ-Resistant Glioblastoma. [Abstract]2025 Dec 27:e13632. PMID: 41454694 -
Adv Sci (Weinh)
Microfilament-Myosin II Regulates the Differentiation of Multinucleated Cysts into Oocytes and Influences Oocyte Developmental Potential in Mice. [Abstract]2025 Sep 17:e00358. PMID: 40959902
CK-666 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 17:e00358. [Abstract]
CK666 (2.5 μg/mL, 4 days) reduced F-actin polymerization in E15.5 fetal mice.
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Cell Death Dis
UBC9-mediated SUMOylation of CORO1C drives lung adenocarcinoma progression via Arp2/3-dependent cytoskeletal remodeling. [Abstract]2026 Mar 30;17(1):434. PMID: 41912501 -
Cell Death Dis
EZH2 reduction is an essential mechanoresponse for the maintenance of super-enhancer polarization against compressive stress in human periodontal ligament stem cells. [Abstract]2020 Sep 15;11(9):757. PMID: 32934212 -
Small
Topographic Cues Guiding Cell Polarization via Distinct Cellular Mechanosensing Pathways. [Abstract]2021 Nov 5;e2104328. PMID: 34738726 -
Neural Regen Res
2025 Oct 30. PMID: 41169225 -
NPJ Parkinsons Dis
2024 Apr 9;10(1):80. PMID: 38594264
CK-666 purchased from MedChemExpress. Usage Cited in: NPJ Parkinsons Dis. 2024 Apr 9;10(1):80. [Abstract]
CK666 (100 μM, 24 h) reduced the F-actin/G-actin ratio in doxycycline-treated iPSC-derived neurons.
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Cell Rep
The actin and microtubule network regulator WHAMM is identified as a key kidney disease risk gene. [Abstract]2025 Mar 25;44(4):115462. PMID: 40138314 -
Curr Biol
2025 Apr 7;35(7):1612-1621.e3. PMID: 40049172 -
J Ethnopharmacol
Berberine inhibits intestinal epithelial barrier dysfunction in colon caused by peritoneal dialysis fluid by improving cell migration. [Abstract]2021 Jan 10;264:113206. PMID: 32750460 -
Life Sci
Activation of mannose receptor C type 1 in macrophages improves renal fibrosis through mediating fibronectin endocytosis. [Abstract]2025 Mar 29:123593. PMID: 40164332 -
Int Immunopharmacol
CD9 downregulation activates EGFR/ERK/WAVE2 pathway to remodel F-actin and promote proliferation and migration of cholesteatoma epithelial cells. [Abstract]2025 Aug 25:164:115401. PMID: 40858050 -
iScience
2021 Dec 25;25(1):103676. PMID: 35036867 -
FASEB J
Focal Adhesion Kinase Orchestrates GLUT4 Translocation and Glucose Uptake via Cytoskeletal Turnover in Primary Adipocytes. [Abstract]2025 May 31;39(10):e70660. PMID: 40396386 -
Reproduction
2026 Jan 15;171(1):xaaf013. PMID: 41575147 -
Transbound Emerg Dis
2025 Nov 21:2025:5822897. PMID: 41323147 -
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Solvent & Solubility
DMSO : 125 mg/mL (421.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 12.5 mg/mL (42.18 mM); Clear solution
This protocol yields a clear solution of ≥ 12.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (125.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 12.5 mg/mL (42.18 mM); Clear solution
This protocol yields a clear solution of ≥ 12.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (125.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Hetrick B, et al. Small molecules CK-666 and CK-869 inhibit actin-related protein 2/3 complex by blocking an activating conformational change. Chem Biol. 2013 May 23;20(5):701-12. [Content Brief]
[2]. Brieuc P Perot, et al. Dendritic Cell Maturation Regulates TSPAN7 Function in HIV-1 Transfer to CD4+ T Lymphocytes. Front Cell Infect Microbiol. 2020 Feb 28;10:70. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3745 mL | 16.8725 mL | 33.7450 mL | 84.3626 mL |
| 5 mM | 0.6749 mL | 3.3745 mL | 6.7490 mL | 16.8725 mL | |
| 10 mM | 0.3375 mL | 1.6873 mL | 3.3745 mL | 8.4363 mL | |
| 15 mM | 0.2250 mL | 1.1248 mL | 2.2497 mL | 5.6242 mL | |
| 20 mM | 0.1687 mL | 0.8436 mL | 1.6873 mL | 4.2181 mL | |
| 25 mM | 0.1350 mL | 0.6749 mL | 1.3498 mL | 3.3745 mL | |
| 30 mM | 0.1125 mL | 0.5624 mL | 1.1248 mL | 2.8121 mL | |
| 40 mM | 0.0844 mL | 0.4218 mL | 0.8436 mL | 2.1091 mL | |
| 50 mM | 0.0675 mL | 0.3375 mL | 0.6749 mL | 1.6873 mL | |
| 60 mM | 0.0562 mL | 0.2812 mL | 0.5624 mL | 1.4060 mL | |
| 80 mM | 0.0422 mL | 0.2109 mL | 0.4218 mL | 1.0545 mL | |
| 100 mM | 0.0337 mL | 0.1687 mL | 0.3375 mL | 0.8436 mL |
CK-666 Related Classifications
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